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The map label for this gene is braC [H]

Identifier: 83310651

GI number: 83310651

Start: 1682205

End: 1683359

Strand: Reverse

Name: braC [H]

Synonym: amb1552

Alternate gene names: 83310651

Gene position: 1683359-1682205 (Counterclockwise)

Preceding gene: 83310653

Following gene: 83310650

Centisome position: 33.89

GC content: 65.71

Gene sequence:

>1155_bases
ATGAATTGGAAACGTACGGCCGGGGCTGCCCTGGCGGTGATGTCGGGTCTTGGCTACGCGGCCTCCGCTCTGGCGGCCGA
CCCCATCAAGATCGGCATGTTCCTGTCCGTCACCGGACAGATGTCGCCCATGGGCGACCCCCAGAAGCGCACCTTCGACT
ACATGATCGAGAAGATCAACGCCCAGGGCGGCGTGCTGGGCCGCAAGGTGGAAGCCGTCATCTATGACGACGGGTCCGAG
CCGGAAAAGGCCGCCACCTTCGTCAAGCGCCTGATCGACAACGACAAGGTCGACCTCATCATCGGCGGGTCGGGCACGCC
CACCTCCATGGCGGTGCTGGGTCTGATCGAACGCGCCGAGGTGCCCTATCTGTCCCTGGGCGGCGGCACCGCCATCGGCG
ACCCGGTGCGCAAGTGGACCTTCAAGTTCCCGCAATCGGACCGGCTGGCCGCCGAGAAGGTTCTGGTGGACCTGAAGAAG
CGCGGCCTGACCAAGATCGCCCTGCTGTCCGAGAATGTCGGCTTCGGCAAGTCCGGTCACGACCAGACCGTCAAGCTGGC
CCCCCAGCACGGCATCGAGATCCTGATCGACGAGGTCTACTCGCCCAAGGATCCCGACGTCACCCCGCAGCTGACCAAGA
TCCGCGGCACTGCGGGCGTCCAGGCCCTGTTCATCTTCGGAACCGGCACCGGCCCGGCCGTGGTGACCAAGAATCTGCGG
CAGATGGGCCTGAACCTGCCCACCTATCAGTCCCACGGCGTGGCCTCCAAGGAGTTCCTGCGTCTGGTCGGCACCGCCGC
CGACGGCATGCGCCTGCCGGCGGGTGCCCAGGCGGTGGCCGAACAGCTACCGGCCAACGATCCGCAGAAGGCGGTGACCA
TGGCCTTCAAGAAGGAATACGAGGAGAAGAACAAGCCGCTGGAAGCCGGCCCCCTGCCCGGTCACGGCCTTGACGCCCTG
ATGATGGCCATGGACGCCTTCAAGCGGGCCGGCACCACCGACAAGGCCAAGGTCCGCGACGCCATCGAGCAGACCAAGGG
CTTCGTCGCCACCACCGGCATCTTCACCTATTCCCCCACCGACCACATGGGCCTGGGCCTCGACGCCTTCCACATGGTCG
ACATCAAGAACGGCGATTGGGTCATCGCCGACTGA

Upstream 100 bases:

>100_bases
GCACTTTTTGGGACTTCGCTTTTAAGCAATTCGGTACTACAATACCGACAAAATAAGAAGTGATAAGCCTGGATAAATCA
TACGAAGTGGGAGGAACTTC

Downstream 100 bases:

>100_bases
TCTTCCGCGTGAAACAGGCGGGTCGCCCACGACGGCGGCCCGCCACCTTCAGGATTTGACCCCATGAATGCTGCCGACGA
AATTCTCGGCCCCAGCCTCG

Product: ABC-type branched-chain amino acid transport systems, periplasmic component

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNWKRTAGAALAVMSGLGYAASALAADPIKIGMFLSVTGQMSPMGDPQKRTFDYMIEKINAQGGVLGRKVEAVIYDDGSE
PEKAATFVKRLIDNDKVDLIIGGSGTPTSMAVLGLIERAEVPYLSLGGGTAIGDPVRKWTFKFPQSDRLAAEKVLVDLKK
RGLTKIALLSENVGFGKSGHDQTVKLAPQHGIEILIDEVYSPKDPDVTPQLTKIRGTAGVQALFIFGTGTGPAVVTKNLR
QMGLNLPTYQSHGVASKEFLRLVGTAADGMRLPAGAQAVAEQLPANDPQKAVTMAFKKEYEEKNKPLEAGPLPGHGLDAL
MMAMDAFKRAGTTDKAKVRDAIEQTKGFVATTGIFTYSPTDHMGLGLDAFHMVDIKNGDWVIAD

Sequences:

>Translated_384_residues
MNWKRTAGAALAVMSGLGYAASALAADPIKIGMFLSVTGQMSPMGDPQKRTFDYMIEKINAQGGVLGRKVEAVIYDDGSE
PEKAATFVKRLIDNDKVDLIIGGSGTPTSMAVLGLIERAEVPYLSLGGGTAIGDPVRKWTFKFPQSDRLAAEKVLVDLKK
RGLTKIALLSENVGFGKSGHDQTVKLAPQHGIEILIDEVYSPKDPDVTPQLTKIRGTAGVQALFIFGTGTGPAVVTKNLR
QMGLNLPTYQSHGVASKEFLRLVGTAADGMRLPAGAQAVAEQLPANDPQKAVTMAFKKEYEEKNKPLEAGPLPGHGLDAL
MMAMDAFKRAGTTDKAKVRDAIEQTKGFVATTGIFTYSPTDHMGLGLDAFHMVDIKNGDWVIAD
>Mature_384_residues
MNWKRTAGAALAVMSGLGYAASALAADPIKIGMFLSVTGQMSPMGDPQKRTFDYMIEKINAQGGVLGRKVEAVIYDDGSE
PEKAATFVKRLIDNDKVDLIIGGSGTPTSMAVLGLIERAEVPYLSLGGGTAIGDPVRKWTFKFPQSDRLAAEKVLVDLKK
RGLTKIALLSENVGFGKSGHDQTVKLAPQHGIEILIDEVYSPKDPDVTPQLTKIRGTAGVQALFIFGTGTGPAVVTKNLR
QMGLNLPTYQSHGVASKEFLRLVGTAADGMRLPAGAQAVAEQLPANDPQKAVTMAFKKEYEEKNKPLEAGPLPGHGLDAL
MMAMDAFKRAGTTDKAKVRDAIEQTKGFVATTGIFTYSPTDHMGLGLDAFHMVDIKNGDWVIAD

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40988; Mature: 40988

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKRTAGAALAVMSGLGYAASALAADPIKIGMFLSVTGQMSPMGDPQKRTFDYMIEKIN
CCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
AQGGVLGRKVEAVIYDDGSEPEKAATFVKRLIDNDKVDLIIGGSGTPTSMAVLGLIERAE
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCC
VPYLSLGGGTAIGDPVRKWTFKFPQSDRLAAEKVLVDLKKRGLTKIALLSENVGFGKSGH
CCEEECCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
DQTVKLAPQHGIEILIDEVYSPKDPDVTPQLTKIRGTAGVQALFIFGTGTGPAVVTKNLR
CCEEEECCCCCCEEEEEHHCCCCCCCCCCHHHEECCCCCEEEEEEEECCCCHHHHHHHHH
QMGLNLPTYQSHGVASKEFLRLVGTAADGMRLPAGAQAVAEQLPANDPQKAVTMAFKKEY
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
EEKNKPLEAGPLPGHGLDALMMAMDAFKRAGTTDKAKVRDAIEQTKGFVATTGIFTYSPT
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEEEEECCC
DHMGLGLDAFHMVDIKNGDWVIAD
CCCCCCCCEEEEEEECCCCEEEEC
>Mature Secondary Structure
MNWKRTAGAALAVMSGLGYAASALAADPIKIGMFLSVTGQMSPMGDPQKRTFDYMIEKIN
CCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
AQGGVLGRKVEAVIYDDGSEPEKAATFVKRLIDNDKVDLIIGGSGTPTSMAVLGLIERAE
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCC
VPYLSLGGGTAIGDPVRKWTFKFPQSDRLAAEKVLVDLKKRGLTKIALLSENVGFGKSGH
CCEEECCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
DQTVKLAPQHGIEILIDEVYSPKDPDVTPQLTKIRGTAGVQALFIFGTGTGPAVVTKNLR
CCEEEECCCCCCEEEEEHHCCCCCCCCCCHHHEECCCCCEEEEEEEECCCCHHHHHHHHH
QMGLNLPTYQSHGVASKEFLRLVGTAADGMRLPAGAQAVAEQLPANDPQKAVTMAFKKEY
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
EEKNKPLEAGPLPGHGLDALMMAMDAFKRAGTTDKAKVRDAIEQTKGFVATTGIFTYSPT
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEEEEECCC
DHMGLGLDAFHMVDIKNGDWVIAD
CCCCCCCCEEEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]