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The map label for this gene is hcl [H]

Identifier: 83310650

GI number: 83310650

Start: 1680639

End: 1682141

Strand: Reverse

Name: hcl [H]

Synonym: amb1551

Alternate gene names: 83310650

Gene position: 1682141-1680639 (Counterclockwise)

Preceding gene: 83310651

Following gene: 83310649

Centisome position: 33.87

GC content: 68.8

Gene sequence:

>1503_bases
ATGAATGCTGCCGACGAAATTCTCGGCCCCAGCCTCGGCTCGGGTCGCGGGGAGAGCCTTGCCATCATCTGCGGCGAGCA
GACCCTGACCTACGACCAGTTGAACCGGCTGGCTTGCCGTTTCGGCAACGCCATGCTGGCCGCCGGCGTCCAGCGCCAGC
AGCCCGTCCTGCTGCTGCTCGACGATGGCCCCGAACTGGTGGCCGCCTATCTGGGCGCCATGAAGGCCGGGTTGGTGGCG
GTGGCTCTCAATACCCGCCTGTCGCCCAAGGACCTGTCCCATGCGCTGGGCGACAGCGGCGCGCCGCTGCTGCTGGCCGA
GACCGCCCTGAAGGATCTGGCCGCCGAATCCCTGGCCCTGGCCCACGCCTCGGCCCGCATGGTAACCACCGATGAACTGG
ACGCCTTCCTGGAAGGCGCGTCCGACCAGTTGGTCTCGGCCGACATGGGGCCGGAGGACATGGCCCTGTGGATGTACACG
TCCGGCACCACCGGCCAGCCCAAGGGCGCCGTGCATGTCCATGGCTCCATTCCCTTGGGCGAGCGCCATGTGCGGGAGAA
TCTCGGCCTTTTGCCCGGCGACCGCATCTTTTCCACCTCCAAGCTGTTCTTCGCCTATCCCCTGGGCCATTGCCTGATCG
GGGCATTGCGCTGCGGCGGCACCCTGGTGCTGCATCGCGGCTGGCCCGATGCGACGGCCGCCGCCGAGGTCATCGCCCGC
ACCCGGCCCAAGCTGGTCCTGTCGGTCCCCTCGCTCTACCGCATCATGCTGAAGGACGGCGTGGGGTCGTCCCCAGCCTT
CCGCGAGGTCCGCACCTGGGTCTCGGCGGGCGAGAACCTGCCGGCCGATCTGTGCCGCCGCTGGATGGAGGAAACCGGCG
GCCTGATGCTCGAAGGCATCGGCGCCACCGAGGCCCTGTTCCTATTCATCGCCAGCACGCCGACGGCCATGAAGCCCGGC
GCCTGCGGCCGTCCCCTGCCTTGGGCCGAGGCACAGTTGCGCTCGCCATCGGGCGAAGTCATCGCCGCGCCCGATACCCC
CGGCGATTTGTGGGTGCGCATGGATTCCCTGTTCCGCCGCTACCACAACCGCCCCGACGTCACCCAGCGGGTGCTGAAGG
ACGGCTGGTGGAAGACCGGCGACGTCTTCAGCTTCGATGCCGAGGGCTGGTGGAGCCCCCAGGGGCGCTCCGACGACATG
ATCAAGGTCTCCGGCCAGTGGGTCAGCCCGTCCGAGGTGGAGGAAGCCGCCCTGATGGTGCCCGGCGTCGCCGATGCCGT
GGCGGTGGGCATTCCCAACGAGGACGGGCTGGTGCGTCTGGTGCTCTACGCCGTGGCCGAGGCGGGCGAGCACGAGCCCC
TCCTGGAAACCCGCATCGTCGAGACCCTGCGCTCCAAGCTGGCCATCTACAAATGCCCGCGCAATGTCCGCTTCCTGGAA
ACCATCCCCCGCACCGCCACCGGCAAGGTGCAGCGGTTCAAGCTGAGAGAGGCGGGGGCCTGA

Upstream 100 bases:

>100_bases
CGACATCAAGAACGGCGATTGGGTCATCGCCGACTGATCTTCCGCGTGAAACAGGCGGGTCGCCCACGACGGCGGCCCGC
CACCTTCAGGATTTGACCCC

Downstream 100 bases:

>100_bases
GACGATACGGGGCTCCCGCCCCGTAACCCCGGCCGGAGGCGATGCCTCCGGACCTCTCTTTGATTTATGGGTATAAAGGG
GGTCCGGGGCATCGCCCCGG

Product: Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase

Products: NA

Alternate protein names: 3-hydroxybenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNAADEILGPSLGSGRGESLAIICGEQTLTYDQLNRLACRFGNAMLAAGVQRQQPVLLLLDDGPELVAAYLGAMKAGLVA
VALNTRLSPKDLSHALGDSGAPLLLAETALKDLAAESLALAHASARMVTTDELDAFLEGASDQLVSADMGPEDMALWMYT
SGTTGQPKGAVHVHGSIPLGERHVRENLGLLPGDRIFSTSKLFFAYPLGHCLIGALRCGGTLVLHRGWPDATAAAEVIAR
TRPKLVLSVPSLYRIMLKDGVGSSPAFREVRTWVSAGENLPADLCRRWMEETGGLMLEGIGATEALFLFIASTPTAMKPG
ACGRPLPWAEAQLRSPSGEVIAAPDTPGDLWVRMDSLFRRYHNRPDVTQRVLKDGWWKTGDVFSFDAEGWWSPQGRSDDM
IKVSGQWVSPSEVEEAALMVPGVADAVAVGIPNEDGLVRLVLYAVAEAGEHEPLLETRIVETLRSKLAIYKCPRNVRFLE
TIPRTATGKVQRFKLREAGA

Sequences:

>Translated_500_residues
MNAADEILGPSLGSGRGESLAIICGEQTLTYDQLNRLACRFGNAMLAAGVQRQQPVLLLLDDGPELVAAYLGAMKAGLVA
VALNTRLSPKDLSHALGDSGAPLLLAETALKDLAAESLALAHASARMVTTDELDAFLEGASDQLVSADMGPEDMALWMYT
SGTTGQPKGAVHVHGSIPLGERHVRENLGLLPGDRIFSTSKLFFAYPLGHCLIGALRCGGTLVLHRGWPDATAAAEVIAR
TRPKLVLSVPSLYRIMLKDGVGSSPAFREVRTWVSAGENLPADLCRRWMEETGGLMLEGIGATEALFLFIASTPTAMKPG
ACGRPLPWAEAQLRSPSGEVIAAPDTPGDLWVRMDSLFRRYHNRPDVTQRVLKDGWWKTGDVFSFDAEGWWSPQGRSDDM
IKVSGQWVSPSEVEEAALMVPGVADAVAVGIPNEDGLVRLVLYAVAEAGEHEPLLETRIVETLRSKLAIYKCPRNVRFLE
TIPRTATGKVQRFKLREAGA
>Mature_500_residues
MNAADEILGPSLGSGRGESLAIICGEQTLTYDQLNRLACRFGNAMLAAGVQRQQPVLLLLDDGPELVAAYLGAMKAGLVA
VALNTRLSPKDLSHALGDSGAPLLLAETALKDLAAESLALAHASARMVTTDELDAFLEGASDQLVSADMGPEDMALWMYT
SGTTGQPKGAVHVHGSIPLGERHVRENLGLLPGDRIFSTSKLFFAYPLGHCLIGALRCGGTLVLHRGWPDATAAAEVIAR
TRPKLVLSVPSLYRIMLKDGVGSSPAFREVRTWVSAGENLPADLCRRWMEETGGLMLEGIGATEALFLFIASTPTAMKPG
ACGRPLPWAEAQLRSPSGEVIAAPDTPGDLWVRMDSLFRRYHNRPDVTQRVLKDGWWKTGDVFSFDAEGWWSPQGRSDDM
IKVSGQWVSPSEVEEAALMVPGVADAVAVGIPNEDGLVRLVLYAVAEAGEHEPLLETRIVETLRSKLAIYKCPRNVRFLE
TIPRTATGKVQRFKLREAGA

Specific function: Catalyzes the ligation of 3-hydroxybenzoate or 4- hydroxybenzoate and CoA at the expense of ATP. The enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4- chlorobenzoate.

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=495, Percent_Identity=27.2727272727273, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI58082049, Length=469, Percent_Identity=25.7995735607676, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI157311624, Length=469, Percent_Identity=25.5863539445629, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI157311622, Length=469, Percent_Identity=25.5863539445629, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI122937307, Length=492, Percent_Identity=25.2032520325203, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI115511026, Length=499, Percent_Identity=26.8537074148297, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI38505220, Length=499, Percent_Identity=25.4509018036072, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI156151445, Length=523, Percent_Identity=25.2390057361377, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI187761345, Length=518, Percent_Identity=25.2895752895753, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI187761343, Length=518, Percent_Identity=25.2895752895753, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28416953, Length=514, Percent_Identity=24.5136186770428, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI42544134, Length=334, Percent_Identity=25.4491017964072, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI8923896, Length=554, Percent_Identity=24.0072202166065, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI227499619, Length=521, Percent_Identity=24.7600767754319, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI38524616, Length=520, Percent_Identity=26.7307692307692, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI13375727, Length=569, Percent_Identity=23.9015817223199, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI253970489, Length=393, Percent_Identity=24.6819338422392, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI13236579, Length=492, Percent_Identity=26.4227642276423, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI195947378, Length=368, Percent_Identity=21.4673913043478, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788107, Length=522, Percent_Identity=27.2030651340996, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1790505, Length=532, Percent_Identity=24.812030075188, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI145693145, Length=490, Percent_Identity=25.9183673469388, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=24.6601941747573, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789201, Length=396, Percent_Identity=27.2727272727273, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786810, Length=517, Percent_Identity=25.9187620889749, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1786801, Length=388, Percent_Identity=26.8041237113402, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=26.3565891472868, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=23, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71996755, Length=493, Percent_Identity=24.5436105476673, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=520, Percent_Identity=25.7692307692308, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=520, Percent_Identity=25.7692307692308, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=520, Percent_Identity=25.7692307692308, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=472, Percent_Identity=24.5762711864407, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=515, Percent_Identity=23.495145631068, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=392, Percent_Identity=25.2551020408163, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115534440, Length=523, Percent_Identity=22.7533460803059, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=366, Percent_Identity=25.4098360655738, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=377, Percent_Identity=23.6074270557029, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=560, Percent_Identity=22.5, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=560, Percent_Identity=22.5, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=524, Percent_Identity=24.4274809160305, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323182, Length=531, Percent_Identity=23.5404896421846, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=522, Percent_Identity=22.6053639846743, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320852, Length=201, Percent_Identity=29.3532338308458, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24581924, Length=469, Percent_Identity=25.1599147121535, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45550496, Length=535, Percent_Identity=25.2336448598131, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45552801, Length=535, Percent_Identity=25.2336448598131, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI62472339, Length=538, Percent_Identity=23.7918215613383, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667955, Length=539, Percent_Identity=24.30426716141, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355181, Length=481, Percent_Identity=20.997920997921, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18859661, Length=510, Percent_Identity=25.4901960784314, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364798, Length=528, Percent_Identity=23.8636363636364, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583465, Length=529, Percent_Identity=24.1965973534972, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583463, Length=529, Percent_Identity=24.1965973534972, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24656500, Length=367, Percent_Identity=26.158038147139, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355225, Length=563, Percent_Identity=22.7353463587922, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922652, Length=497, Percent_Identity=21.7303822937626, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24667959, Length=503, Percent_Identity=23.8568588469185, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=354, Percent_Identity=24.2937853107345, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.27 [H]

Molecular weight: Translated: 53795; Mature: 53795

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAADEILGPSLGSGRGESLAIICGEQTLTYDQLNRLACRFGNAMLAAGVQRQQPVLLLL
CCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEE
DDGPELVAAYLGAMKAGLVAVALNTRLSPKDLSHALGDSGAPLLLAETALKDLAAESLAL
CCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
AHASARMVTTDELDAFLEGASDQLVSADMGPEDMALWMYTSGTTGQPKGAVHVHGSIPLG
HHHCCCEEEHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCC
ERHVRENLGLLPGDRIFSTSKLFFAYPLGHCLIGALRCGGTLVLHRGWPDATAAAEVIAR
HHHHHHHCCCCCCCCEECCCCEEEEHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
TRPKLVLSVPSLYRIMLKDGVGSSPAFREVRTWVSAGENLPADLCRRWMEETGGLMLEGI
CCCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEC
GATEALFLFIASTPTAMKPGACGRPLPWAEAQLRSPSGEVIAAPDTPGDLWVRMDSLFRR
CCHHEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHH
YHNRPDVTQRVLKDGWWKTGDVFSFDAEGWWSPQGRSDDMIKVSGQWVSPSEVEEAALMV
HCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHEEC
PGVADAVAVGIPNEDGLVRLVLYAVAEAGEHEPLLETRIVETLRSKLAIYKCPRNVRFLE
CCCHHHEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHH
TIPRTATGKVQRFKLREAGA
HCCCCCCCCHHEEEECCCCC
>Mature Secondary Structure
MNAADEILGPSLGSGRGESLAIICGEQTLTYDQLNRLACRFGNAMLAAGVQRQQPVLLLL
CCCHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEE
DDGPELVAAYLGAMKAGLVAVALNTRLSPKDLSHALGDSGAPLLLAETALKDLAAESLAL
CCCHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHH
AHASARMVTTDELDAFLEGASDQLVSADMGPEDMALWMYTSGTTGQPKGAVHVHGSIPLG
HHHCCCEEEHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCC
ERHVRENLGLLPGDRIFSTSKLFFAYPLGHCLIGALRCGGTLVLHRGWPDATAAAEVIAR
HHHHHHHCCCCCCCCEECCCCEEEEHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
TRPKLVLSVPSLYRIMLKDGVGSSPAFREVRTWVSAGENLPADLCRRWMEETGGLMLEGI
CCCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEC
GATEALFLFIASTPTAMKPGACGRPLPWAEAQLRSPSGEVIAAPDTPGDLWVRMDSLFRR
CCHHEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHH
YHNRPDVTQRVLKDGWWKTGDVFSFDAEGWWSPQGRSDDMIKVSGQWVSPSEVEEAALMV
HCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHEEC
PGVADAVAVGIPNEDGLVRLVLYAVAEAGEHEPLLETRIVETLRSKLAIYKCPRNVRFLE
CCCHHHEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHH
TIPRTATGKVQRFKLREAGA
HCCCCCCCCHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11208796 [H]