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The map label for this gene is tktA [H]

Identifier: 83310480

GI number: 83310480

Start: 1497528

End: 1499501

Strand: Reverse

Name: tktA [H]

Synonym: amb1381

Alternate gene names: 83310480

Gene position: 1499501-1497528 (Counterclockwise)

Preceding gene: 83310481

Following gene: 83310479

Centisome position: 30.19

GC content: 67.83

Gene sequence:

>1974_bases
ATGACCACCCACGCCGCAATGGCCAATGCCATCCGCTTTTTGACCATGGACGCCATCGAGAAGGCCAAATCCGGCCATCC
GGGAATGCCCATGGGCATGGCCGATGTGGCCACCGTGCTGTTCACGCGCTTTCTCAAATACGACGCCAAGGCCCCCAAAT
GGGCCGACCGCGACCGCTTCATCCTGTCGGCCGGGCACGGCTCCATGCTGCTCTACGCGCTGGGCTACCTGACCGGCTTC
GAGGATCTGTCCGATATCGAGCAGATCAAGAACTTCCGCCAGATGGGCTACCGCACTGCCGGCCACCCGGAATTCGGCCA
TGTGGCCATCGCCGAGACCACCACCGGCCCGCTGGGCCAGGGCGTCGCCAACGCCGTGGGCTTCGCCCTGGCCGAGAAGA
TGGCCGCCGCCCGCCACGGCAAGGACGTGGTCGACCACTACACCTATGTCATGGCCGGTGACGGCTGCCTGATGGAGGGC
ATCAGCCAGGAAGCCATCTCCCTGGCCGGGGTGTGGAACCTGTCCAAGCTGATCCTGCTGTGGGACGACAACCACATCTG
CATCGACGGCGACACTGCCATTTCCACCTCGGACGACCAGCTGGCCCGCTTCCAGGCGTCCAACTGGGATGTGTGCCGCG
TCGACGGCCATGACCCCGAGGCCATCGCCACCGCCATCGCCGCGGCGCAGAAGTCCGGCCAGCCCAGCCTGATCGCCTGC
CGCACCACCATCGGCAAGGGCGCTCCCACCAAGGCGGGCAGCGAGAAGGTCCACGGCGCCCCGCTGGGCGGCGACGAGAT
CGCCGCGGCGCGCGCCGCCGCCGGCTGGAGCGCCGGTCCCTTCGAGATTCCCGCCGATGTGCTGGACGCCTGGCGCGCCG
CCGGCACCAAGGGCGCCAGCGCCCGCGCCGAGTGGGAGAAGCGCTTCGCCGCCTATGCGGGCAAGGACGAGTTCCTGCGC
ACCAATGCCGGCACCCTGCCCCAGGGCTGGAAGCAGGCGGTGTCGGCCTTCAAGCAGAAGGTTTCGGCCGAGCAGCCCAA
ATGGGCCACCCGCAAGGCCGGCCAGGAAGTGCTCGAAGCCCTGACCCCCGTCATCCCCGAGATGATCGGCGGCTCGGCCG
ACCTCACCCACTCGAACCTGACCATCACCAAGGCCACCAAGCCGGTTTCGGCCTCGGACTGCTCGGGCCGCTACATCCAC
TACGGCGTGCGCGAGCACGGCATGGCCTCGATCATGAACGGCCTGTCGCTGCATGGGGGCTTCATCCCCTATGGCGGCAC
CTTCCTGATCTTCGCCAACTATCTGTGGCCGGCGCTGCGCATGAGCGCCATGATGGACCAGCGGGTGCTCTACGTGCTGA
CCCACGATTCCATCGGCCTGGGCGAGGACGGCCCCACCCACCAGCCCATCGAGACCGTCGCCGCCCTGCGCGCCACCCCC
AATGTGCTGGTGTTCCGCCCCGCCGATCCGGTGGAGACCATGGAGGCCTACGAGGCCGCCCTGGACAATGCCAAGGGGCC
GTCGGTCTTCGCCCTGTCGCGCCAGAACCTGCCGACGCTGCGCACCACCCACACCGACGAGAACCTCACCGCCAAGGGCG
CCTATGTGCTGGCCGAGGCCGAGGGAAAGAAGCGTCAGGTCACCCTGATCGCCACCGGCTCGGAAGTCAGCCTGGCCATG
GAGGCGCGCAAGCTGCTGGCCGACAAGGGTATCGCGGCGGCCGTGGTCTCCATGCCCTGCTGGGAACTGTTCGAACGCCA
GCCGAAGGAGTATCGTAACTCCGTGTTGGGTGAAGGCACCGTGCGGGTCGCCGTCGAGGCGCTGGGCACCTTCGGGTGGG
AGCGCTGGGTCGGCGAAAACGGCGCCGTCATCGGCATGAACGGCTTTGGAGCCTCGGCACCCGCCGAGAAACTCTACGAG
CATTTCGGCATTACCGCCCAAGCGGTGGCCGACGCGGCCCAGGCTCGCCTCTAA

Upstream 100 bases:

>100_bases
CCAGACCCTGTTGATCGGAGGGGCCGCCGGGCCTCGCCAGATGTTGACCTCGTGGCACCGCCGCGCCTGACTTGAACCCT
TCACCCTTGAGGTTTGCTCG

Downstream 100 bases:

>100_bases
GACGACCACGGCGGCAAAACGCCAGGAAGGAGATGAAGTAGATGTCCCTTGTTTCGATGCGCCAGCTCCTCGACCACGCT
GCCGAGAATAGCTACGGCAT

Product: transketolase

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MTTHAAMANAIRFLTMDAIEKAKSGHPGMPMGMADVATVLFTRFLKYDAKAPKWADRDRFILSAGHGSMLLYALGYLTGF
EDLSDIEQIKNFRQMGYRTAGHPEFGHVAIAETTTGPLGQGVANAVGFALAEKMAAARHGKDVVDHYTYVMAGDGCLMEG
ISQEAISLAGVWNLSKLILLWDDNHICIDGDTAISTSDDQLARFQASNWDVCRVDGHDPEAIATAIAAAQKSGQPSLIAC
RTTIGKGAPTKAGSEKVHGAPLGGDEIAAARAAAGWSAGPFEIPADVLDAWRAAGTKGASARAEWEKRFAAYAGKDEFLR
TNAGTLPQGWKQAVSAFKQKVSAEQPKWATRKAGQEVLEALTPVIPEMIGGSADLTHSNLTITKATKPVSASDCSGRYIH
YGVREHGMASIMNGLSLHGGFIPYGGTFLIFANYLWPALRMSAMMDQRVLYVLTHDSIGLGEDGPTHQPIETVAALRATP
NVLVFRPADPVETMEAYEAALDNAKGPSVFALSRQNLPTLRTTHTDENLTAKGAYVLAEAEGKKRQVTLIATGSEVSLAM
EARKLLADKGIAAAVVSMPCWELFERQPKEYRNSVLGEGTVRVAVEALGTFGWERWVGENGAVIGMNGFGASAPAEKLYE
HFGITAQAVADAAQARL

Sequences:

>Translated_657_residues
MTTHAAMANAIRFLTMDAIEKAKSGHPGMPMGMADVATVLFTRFLKYDAKAPKWADRDRFILSAGHGSMLLYALGYLTGF
EDLSDIEQIKNFRQMGYRTAGHPEFGHVAIAETTTGPLGQGVANAVGFALAEKMAAARHGKDVVDHYTYVMAGDGCLMEG
ISQEAISLAGVWNLSKLILLWDDNHICIDGDTAISTSDDQLARFQASNWDVCRVDGHDPEAIATAIAAAQKSGQPSLIAC
RTTIGKGAPTKAGSEKVHGAPLGGDEIAAARAAAGWSAGPFEIPADVLDAWRAAGTKGASARAEWEKRFAAYAGKDEFLR
TNAGTLPQGWKQAVSAFKQKVSAEQPKWATRKAGQEVLEALTPVIPEMIGGSADLTHSNLTITKATKPVSASDCSGRYIH
YGVREHGMASIMNGLSLHGGFIPYGGTFLIFANYLWPALRMSAMMDQRVLYVLTHDSIGLGEDGPTHQPIETVAALRATP
NVLVFRPADPVETMEAYEAALDNAKGPSVFALSRQNLPTLRTTHTDENLTAKGAYVLAEAEGKKRQVTLIATGSEVSLAM
EARKLLADKGIAAAVVSMPCWELFERQPKEYRNSVLGEGTVRVAVEALGTFGWERWVGENGAVIGMNGFGASAPAEKLYE
HFGITAQAVADAAQARL
>Mature_656_residues
TTHAAMANAIRFLTMDAIEKAKSGHPGMPMGMADVATVLFTRFLKYDAKAPKWADRDRFILSAGHGSMLLYALGYLTGFE
DLSDIEQIKNFRQMGYRTAGHPEFGHVAIAETTTGPLGQGVANAVGFALAEKMAAARHGKDVVDHYTYVMAGDGCLMEGI
SQEAISLAGVWNLSKLILLWDDNHICIDGDTAISTSDDQLARFQASNWDVCRVDGHDPEAIATAIAAAQKSGQPSLIACR
TTIGKGAPTKAGSEKVHGAPLGGDEIAAARAAAGWSAGPFEIPADVLDAWRAAGTKGASARAEWEKRFAAYAGKDEFLRT
NAGTLPQGWKQAVSAFKQKVSAEQPKWATRKAGQEVLEALTPVIPEMIGGSADLTHSNLTITKATKPVSASDCSGRYIHY
GVREHGMASIMNGLSLHGGFIPYGGTFLIFANYLWPALRMSAMMDQRVLYVLTHDSIGLGEDGPTHQPIETVAALRATPN
VLVFRPADPVETMEAYEAALDNAKGPSVFALSRQNLPTLRTTHTDENLTAKGAYVLAEAEGKKRQVTLIATGSEVSLAME
ARKLLADKGIAAAVVSMPCWELFERQPKEYRNSVLGEGTVRVAVEALGTFGWERWVGENGAVIGMNGFGASAPAEKLYEH
FGITAQAVADAAQARL

Specific function: Unknown

COG id: COG0021

COG function: function code G; Transketolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI205277463, Length=667, Percent_Identity=26.0869565217391, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI4507521, Length=667, Percent_Identity=26.0869565217391, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI133778974, Length=582, Percent_Identity=26.9759450171821, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI225637459, Length=669, Percent_Identity=21.52466367713, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI225637461, Length=641, Percent_Identity=21.216848673947, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI225637463, Length=638, Percent_Identity=21.3166144200627, Blast_Score=89, Evalue=2e-17,
Organism=Escherichia coli, GI48994911, Length=668, Percent_Identity=50.8982035928144, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1788808, Length=662, Percent_Identity=50.4531722054381, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17539652, Length=571, Percent_Identity=26.0945709281961, Blast_Score=152, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6325331, Length=668, Percent_Identity=46.1077844311377, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6319593, Length=669, Percent_Identity=42.8998505231689, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24666278, Length=259, Percent_Identity=33.2046332046332, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45551847, Length=259, Percent_Identity=32.4324324324324, Blast_Score=122, Evalue=9e-28,
Organism=Drosophila melanogaster, GI45550715, Length=259, Percent_Identity=32.4324324324324, Blast_Score=122, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24645119, Length=228, Percent_Identity=32.8947368421053, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005478
- InterPro:   IPR020826
- InterPro:   IPR005476
- InterPro:   IPR005474 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 70181; Mature: 70050

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00801 TRANSKETOLASE_1 ; PS00802 TRANSKETOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHAAMANAIRFLTMDAIEKAKSGHPGMPMGMADVATVLFTRFLKYDAKAPKWADRDRF
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
ILSAGHGSMLLYALGYLTGFEDLSDIEQIKNFRQMGYRTAGHPEFGHVAIAETTTGPLGQ
EEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCH
GVANAVGFALAEKMAAARHGKDVVDHYTYVMAGDGCLMEGISQEAISLAGVWNLSKLILL
HHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCHHHHCCHHHHHHHHEEECCEEEEEE
WDDNHICIDGDTAISTSDDQLARFQASNWDVCRVDGHDPEAIATAIAAAQKSGQPSLIAC
ECCCEEEECCCCCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEE
RTTIGKGAPTKAGSEKVHGAPLGGDEIAAARAAAGWSAGPFEIPADVLDAWRAAGTKGAS
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCC
ARAEWEKRFAAYAGKDEFLRTNAGTLPQGWKQAVSAFKQKVSAEQPKWATRKAGQEVLEA
HHHHHHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LTPVIPEMIGGSADLTHSNLTITKATKPVSASDCSGRYIHYGVREHGMASIMNGLSLHGG
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECHHHCCHHHHHHCHHHCCC
FIPYGGTFLIFANYLWPALRMSAMMDQRVLYVLTHDSIGLGEDGPTHQPIETVAALRATP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCC
NVLVFRPADPVETMEAYEAALDNAKGPSVFALSRQNLPTLRTTHTDENLTAKGAYVLAEA
CEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECCCCCCCEECCEEEEEEC
EGKKRQVTLIATGSEVSLAMEARKLLADKGIAAAVVSMPCWELFERQPKEYRNSVLGEGT
CCCCEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
VRVAVEALGTFGWERWVGENGAVIGMNGFGASAPAEKLYEHFGITAQAVADAAQARL
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTHAAMANAIRFLTMDAIEKAKSGHPGMPMGMADVATVLFTRFLKYDAKAPKWADRDRF
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
ILSAGHGSMLLYALGYLTGFEDLSDIEQIKNFRQMGYRTAGHPEFGHVAIAETTTGPLGQ
EEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCH
GVANAVGFALAEKMAAARHGKDVVDHYTYVMAGDGCLMEGISQEAISLAGVWNLSKLILL
HHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCHHHHCCHHHHHHHHEEECCEEEEEE
WDDNHICIDGDTAISTSDDQLARFQASNWDVCRVDGHDPEAIATAIAAAQKSGQPSLIAC
ECCCEEEECCCCCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEE
RTTIGKGAPTKAGSEKVHGAPLGGDEIAAARAAAGWSAGPFEIPADVLDAWRAAGTKGAS
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCC
ARAEWEKRFAAYAGKDEFLRTNAGTLPQGWKQAVSAFKQKVSAEQPKWATRKAGQEVLEA
HHHHHHHHHHHHCCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LTPVIPEMIGGSADLTHSNLTITKATKPVSASDCSGRYIHYGVREHGMASIMNGLSLHGG
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECHHHCCHHHHHHCHHHCCC
FIPYGGTFLIFANYLWPALRMSAMMDQRVLYVLTHDSIGLGEDGPTHQPIETVAALRATP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCC
NVLVFRPADPVETMEAYEAALDNAKGPSVFALSRQNLPTLRTTHTDENLTAKGAYVLAEA
CEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECCCCCCCEECCEEEEEEC
EGKKRQVTLIATGSEVSLAMEARKLLADKGIAAAVVSMPCWELFERQPKEYRNSVLGEGT
CCCCEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
VRVAVEALGTFGWERWVGENGAVIGMNGFGASAPAEKLYEHFGITAQAVADAAQARL
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8635754; 9256491 [H]