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The map label for this gene is fba [H]

Identifier: 83310479

GI number: 83310479

Start: 1496422

End: 1497486

Strand: Reverse

Name: fba [H]

Synonym: amb1380

Alternate gene names: 83310479

Gene position: 1497486-1496422 (Counterclockwise)

Preceding gene: 83310480

Following gene: 83310478

Centisome position: 30.15

GC content: 64.98

Gene sequence:

>1065_bases
ATGTCCCTTGTTTCGATGCGCCAGCTCCTCGACCACGCTGCCGAGAATAGCTACGGCATTCCCGCCTTCAACGTGAACAA
CATGGAGCAGGTCAAGGCGATCATGGAGGCGGCGTCCGCCTGCGACGCCCCGGTCATCCTCCAGGCCTCGGCCGGCGCCC
GCAAATACGCCGGCGAGCCCTTCCTGCGCCACCTGATCCTGGCCGCCATCGAGGCCTATCCCCAGATTCCGGTCTGCATG
CACCAGGACCACGGCACCAGCCCGGCCATCAATGTGCGCGCCATCCAGTCCGGCTTCTCGTCGGTGATGATGGACGGCTC
GCTCCGGGAAGACGGCAAGACCCCGTCCGATTGGGATTACAACGTGGCGGTCACCAAGCAGGTGGTCGACATCGCCCATG
CCTGCGGCGTCTCGGTCGAGGGTGAGCTGGGCTGTCTCGGCTCGCTGGAAACCGGCACCGCCGGCAAGGAAGACGGCGTC
GGCGCCGAGGGCAAGCTGGACCATTCCCAGCTGCTGACCGACCCTGACGAGGCCGCCGAATTCGTCAAGCTGACCCAGGT
GGATGCCCTGGCCATCGCCATCGGCACCAGCCACGGCGCCTACAAGTTCACCCGTCCGCCGACGGGCGAAGTGCTGGCCA
TCAAGCGCATCAAGGAAATCCACGCCCGCATCCCCAACACCCATCTGGTGATGCACGGCTCCTCCTCGGTGCCTCAGGAC
CTGCTGAAGATCGTCAACGATTACGGCGGCGCCCTGGGCGAGACCTATGGCGTGCCGGTCGGGGAAATCGTCGAAGGCAT
CAAGCACGGCGTGCGCAAGATCAACATCGACACCGACCTGCGTCTGGCCGTCACCGGCTGCATCCGCAAGTACTTCCACG
ACAACCCCAAGAAGTTCGACCCCCGCGACTACCTGAAGCCCTCCATCGCCGCCATGGTCGAGGTCTGCAAGAGCCGCTAC
GACGCCTTCGGCACCTCCGGCCAGGCCTCGAAGATCAAGGTGGTCGGCCTGGAAGACATGAGCGCGCTGTATGCCAAGGG
CGCCCTGGCTCCCAAGGTGAACTGA

Upstream 100 bases:

>100_bases
ACGAGCATTTCGGCATTACCGCCCAAGCGGTGGCCGACGCGGCCCAGGCTCGCCTCTAAGACGACCACGGCGGCAAAACG
CCAGGAAGGAGATGAAGTAG

Downstream 100 bases:

>100_bases
GAAGCTTCTATCATTTCGTCACCCCCGGGCCACCGGGTCTGGCCGAAAGGCCAGCCCGGCGGTGAACTTCACGCCCCGGG
GGTCCCATGGATGGCCGGGT

Product: fructose-1,6-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSLVSMRQLLDHAAENSYGIPAFNVNNMEQVKAIMEAASACDAPVILQASAGARKYAGEPFLRHLILAAIEAYPQIPVCM
HQDHGTSPAINVRAIQSGFSSVMMDGSLREDGKTPSDWDYNVAVTKQVVDIAHACGVSVEGELGCLGSLETGTAGKEDGV
GAEGKLDHSQLLTDPDEAAEFVKLTQVDALAIAIGTSHGAYKFTRPPTGEVLAIKRIKEIHARIPNTHLVMHGSSSVPQD
LLKIVNDYGGALGETYGVPVGEIVEGIKHGVRKINIDTDLRLAVTGCIRKYFHDNPKKFDPRDYLKPSIAAMVEVCKSRY
DAFGTSGQASKIKVVGLEDMSALYAKGALAPKVN

Sequences:

>Translated_354_residues
MSLVSMRQLLDHAAENSYGIPAFNVNNMEQVKAIMEAASACDAPVILQASAGARKYAGEPFLRHLILAAIEAYPQIPVCM
HQDHGTSPAINVRAIQSGFSSVMMDGSLREDGKTPSDWDYNVAVTKQVVDIAHACGVSVEGELGCLGSLETGTAGKEDGV
GAEGKLDHSQLLTDPDEAAEFVKLTQVDALAIAIGTSHGAYKFTRPPTGEVLAIKRIKEIHARIPNTHLVMHGSSSVPQD
LLKIVNDYGGALGETYGVPVGEIVEGIKHGVRKINIDTDLRLAVTGCIRKYFHDNPKKFDPRDYLKPSIAAMVEVCKSRY
DAFGTSGQASKIKVVGLEDMSALYAKGALAPKVN
>Mature_353_residues
SLVSMRQLLDHAAENSYGIPAFNVNNMEQVKAIMEAASACDAPVILQASAGARKYAGEPFLRHLILAAIEAYPQIPVCMH
QDHGTSPAINVRAIQSGFSSVMMDGSLREDGKTPSDWDYNVAVTKQVVDIAHACGVSVEGELGCLGSLETGTAGKEDGVG
AEGKLDHSQLLTDPDEAAEFVKLTQVDALAIAIGTSHGAYKFTRPPTGEVLAIKRIKEIHARIPNTHLVMHGSSSVPQDL
LKIVNDYGGALGETYGVPVGEIVEGIKHGVRKINIDTDLRLAVTGCIRKYFHDNPKKFDPRDYLKPSIAAMVEVCKSRYD
AFGTSGQASKIKVVGLEDMSALYAKGALAPKVN

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=330, Percent_Identity=34.2424242424242, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI87082041, Length=329, Percent_Identity=33.1306990881459, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1788072, Length=330, Percent_Identity=29.6969696969697, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789293, Length=362, Percent_Identity=25.9668508287293, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006412
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 37854; Mature: 37722

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00602 ALDOLASE_CLASS_II_1 ; PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVSMRQLLDHAAENSYGIPAFNVNNMEQVKAIMEAASACDAPVILQASAGARKYAGEP
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCH
FLRHLILAAIEAYPQIPVCMHQDHGTSPAINVRAIQSGFSSVMMDGSLREDGKTPSDWDY
HHHHHHHHHHHHCCCCCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NVAVTKQVVDIAHACGVSVEGELGCLGSLETGTAGKEDGVGAEGKLDHSQLLTDPDEAAE
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
FVKLTQVDALAIAIGTSHGAYKFTRPPTGEVLAIKRIKEIHARIPNTHLVMHGSSSVPQD
HHHHHHHHEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
LLKIVNDYGGALGETYGVPVGEIVEGIKHGVRKINIDTDLRLAVTGCIRKYFHDNPKKFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCC
PRDYLKPSIAAMVEVCKSRYDAFGTSGQASKIKVVGLEDMSALYAKGALAPKVN
CHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLVSMRQLLDHAAENSYGIPAFNVNNMEQVKAIMEAASACDAPVILQASAGARKYAGEP
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCH
FLRHLILAAIEAYPQIPVCMHQDHGTSPAINVRAIQSGFSSVMMDGSLREDGKTPSDWDY
HHHHHHHHHHHHCCCCCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NVAVTKQVVDIAHACGVSVEGELGCLGSLETGTAGKEDGVGAEGKLDHSQLLTDPDEAAE
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
FVKLTQVDALAIAIGTSHGAYKFTRPPTGEVLAIKRIKEIHARIPNTHLVMHGSSSVPQD
HHHHHHHHEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHH
LLKIVNDYGGALGETYGVPVGEIVEGIKHGVRKINIDTDLRLAVTGCIRKYFHDNPKKFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCC
PRDYLKPSIAAMVEVCKSRYDAFGTSGQASKIKVVGLEDMSALYAKGALAPKVN
CHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]