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The map label for this gene is hpcG [H]

Identifier: 83309901

GI number: 83309901

Start: 860107

End: 860913

Strand: Reverse

Name: hpcG [H]

Synonym: amb0802

Alternate gene names: 83309901

Gene position: 860913-860107 (Counterclockwise)

Preceding gene: 83309902

Following gene: 83309900

Centisome position: 17.33

GC content: 67.16

Gene sequence:

>807_bases
ATGGCTCTCACGCTCGATCAGATCAATGCCGCCGCCGCCCGCCTGCATCGGGCCGAGCAGACCCGACACCAGTGCGGCCA
GATTTCGCTGGACCACCCCGAGATGGGCATGGCCGACGCCTACGCGGTGCAAAAGGCTTGGGTCGAGACCAAGATCAATG
AGGGCCGCAAGGTGGTGGGCCACAAGATCGGCCTGACCTCGCGGGCCATGCAGCAATCGTCGCAGATCGACGAGCCCGAT
TACGGCACCCTGCTCGACGACATGGTGTTCGGCGAAGGCACCGACATTCCCATGGCGCGCTTCATCGTCCCCATGGTCGA
GGTGGAACTGGCCTTCATCCTGAAAGACCGGCTGGAAGGCCCCGACGTCACCCTGACCCAGGTGCTGTCGGCCACCGATT
ACGTCATTCCGGCGCTGGAGATCATCGACGCCCGCTGCCACCGCCTCGACCCCGAGACCAAGCGGCCGCGCAAGGTGTTC
GACACCATCTCCGACAACGCCGCCAATGCCGGCATCGTCATGGGCGGCCGGCCGGTCAGGCCCATGGACGTGGACCTGCG
CTGGGTGGCGGCCATCATGTACAGGAACGGGGTGATCGAGGAAACCGGCGTCGCCGCCGGGGTGCTGAACCATCCGGCCC
ACGGCATTTCCTGGCTGGCCCGCAAGTTCGCCCCCCACAGCATCGCGTTGGAGCCGGGGCAGGTGGTGCTGGCCGGCTCC
TTCACTCGCCCGGTGCCGGCGCGGGCCGGCGACACCTTCCATGTGGATTACGGCCCGCTGGGCGCCATCGCCTGCCGGTT
CGTGTAG

Upstream 100 bases:

>100_bases
CAGCGGCTATTTCGCGCTCTCCATGGAGCTCTACGAGTTCGACGAGGGCGGCACCTTCAAGAAGAACAACGTCCACGCCC
GTTTCAGGAAGGCTTGACCC

Downstream 100 bases:

>100_bases
GGCTATCTGACCTGTCATCCCGAGGCGCAAGCCGAGGGATCTCATTCTGGCAGGTCTTTTCATAAACCGGCACCATCGTG
CCAAGCGGAGATCCTTCGCT

Product: 2-keto-4-pentenoate hydratase

Products: NA

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MALTLDQINAAAARLHRAEQTRHQCGQISLDHPEMGMADAYAVQKAWVETKINEGRKVVGHKIGLTSRAMQQSSQIDEPD
YGTLLDDMVFGEGTDIPMARFIVPMVEVELAFILKDRLEGPDVTLTQVLSATDYVIPALEIIDARCHRLDPETKRPRKVF
DTISDNAANAGIVMGGRPVRPMDVDLRWVAAIMYRNGVIEETGVAAGVLNHPAHGISWLARKFAPHSIALEPGQVVLAGS
FTRPVPARAGDTFHVDYGPLGAIACRFV

Sequences:

>Translated_268_residues
MALTLDQINAAAARLHRAEQTRHQCGQISLDHPEMGMADAYAVQKAWVETKINEGRKVVGHKIGLTSRAMQQSSQIDEPD
YGTLLDDMVFGEGTDIPMARFIVPMVEVELAFILKDRLEGPDVTLTQVLSATDYVIPALEIIDARCHRLDPETKRPRKVF
DTISDNAANAGIVMGGRPVRPMDVDLRWVAAIMYRNGVIEETGVAAGVLNHPAHGISWLARKFAPHSIALEPGQVVLAGS
FTRPVPARAGDTFHVDYGPLGAIACRFV
>Mature_267_residues
ALTLDQINAAAARLHRAEQTRHQCGQISLDHPEMGMADAYAVQKAWVETKINEGRKVVGHKIGLTSRAMQQSSQIDEPDY
GTLLDDMVFGEGTDIPMARFIVPMVEVELAFILKDRLEGPDVTLTQVLSATDYVIPALEIIDARCHRLDPETKRPRKVFD
TISDNAANAGIVMGGRPVRPMDVDLRWVAAIMYRNGVIEETGVAAGVLNHPAHGISWLARKFAPHSIALEPGQVVLAGSF
TRPVPARAGDTFHVDYGPLGAIACRFV

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=268, Percent_Identity=34.3283582089552, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.-

Molecular weight: Translated: 29284; Mature: 29153

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTLDQINAAAARLHRAEQTRHQCGQISLDHPEMGMADAYAVQKAWVETKINEGRKVVG
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
HKIGLTSRAMQQSSQIDEPDYGTLLDDMVFGEGTDIPMARFIVPMVEVELAFILKDRLEG
HHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PDVTLTQVLSATDYVIPALEIIDARCHRLDPETKRPRKVFDTISDNAANAGIVMGGRPVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCC
PMDVDLRWVAAIMYRNGVIEETGVAAGVLNHPAHGISWLARKFAPHSIALEPGQVVLAGS
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEECCCCEEEECC
FTRPVPARAGDTFHVDYGPLGAIACRFV
CCCCCCCCCCCEEEECCCCHHHHHEECC
>Mature Secondary Structure 
ALTLDQINAAAARLHRAEQTRHQCGQISLDHPEMGMADAYAVQKAWVETKINEGRKVVG
EEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
HKIGLTSRAMQQSSQIDEPDYGTLLDDMVFGEGTDIPMARFIVPMVEVELAFILKDRLEG
HHCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PDVTLTQVLSATDYVIPALEIIDARCHRLDPETKRPRKVFDTISDNAANAGIVMGGRPVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCC
PMDVDLRWVAAIMYRNGVIEETGVAAGVLNHPAHGISWLARKFAPHSIALEPGQVVLAGS
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCEEEECCCCEEEECC
FTRPVPARAGDTFHVDYGPLGAIACRFV
CCCCCCCCCCCEEEECCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]