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The map label for this gene is hpcH [H]

Identifier: 83309900

GI number: 83309900

Start: 859171

End: 859974

Strand: Reverse

Name: hpcH [H]

Synonym: amb0801

Alternate gene names: 83309900

Gene position: 859974-859171 (Counterclockwise)

Preceding gene: 83309901

Following gene: 83309899

Centisome position: 17.31

GC content: 69.65

Gene sequence:

>804_bases
ATGCCCACACCCGCCAATCCCTTCAAGCGCGCCCTGGCCGAGGGCCGCCCCCAGATCGGCCTGTGGGTTGGCCTTGCCAA
TCCCTATACGGCGGAAATCTGCGCCGGCGCCGGCTTCGACTGGCTGCTGATTGATGGCGAGCACGCCCCCAACGACGTGC
CCGGCCTGCTGGCCCAGTTGCAGGCGGTGGCGCCCTATCCCAGCCACCCCATCGTGCGTCCGGTGATCGGCGACACTGCG
CTGATCAAGCAATTGCTCGATATCGGCGCCCAGACCCTGCTGGTCCCCATGATCGATACGCCGGAACAGGCCGCCGCCAC
GGTGGCGGCCATGCATTACCCCCCCAGGGGCGTGCGCGGCGTGGGCGCCGCCCTGGCGCGGGCCTCGCGCTGGGGACGCA
TCGGCGATTACCTCAAGACCGCGTCGGACGAACTTTGCCTGCTGCTGCAGATCGAGACGGTCACGGGACTGCGCAACCTG
GAGGCCATCGCCACCACCGACGGCGTCGACGGCATCTTCATCGGTCCCGCCGATCTGGCCGCCTCGCTGGGGCATCTGGG
GGCACCATCCCACCCCGAGGTGCAGGCCGAGATCGAGAAGGCCCTGGCGATGCTGAAGCGTCTGGGCGTGCCCAGCGGCA
TCCTGGCCGCCGACGAGACCCTGGCCCGCAAATTTCTGGCGGCGGGGGCGACCTTCGTGGCGGTGGGCATCGACACCATG
GTGCTGAGCCGCGCCCTGTCGGCCCTGGCCGCCACCTACAAGGACGTGGCGCCGCCCGCCCAGACGGATCGTGCCGGCTA
TTAA

Upstream 100 bases:

>100_bases
CGAGGGATCTCATTCTGGCAGGTCTTTTCATAAACCGGCACCATCGTGCCAAGCGGAGATCCTTCGCTACGCTCAGGATG
ACATCAAGGAGAGCCCGCCC

Downstream 100 bases:

>100_bases
GGCTTCAAGCGACATGTCATCCCGACGACCAACGGGAGGAGGGATCTCATCCTGGCACGACGTTTCGGAATTGGCGCCCC
TGCTCCAAGCGGAGATCCCT

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPTPANPFKRALAEGRPQIGLWVGLANPYTAEICAGAGFDWLLIDGEHAPNDVPGLLAQLQAVAPYPSHPIVRPVIGDTA
LIKQLLDIGAQTLLVPMIDTPEQAAATVAAMHYPPRGVRGVGAALARASRWGRIGDYLKTASDELCLLLQIETVTGLRNL
EAIATTDGVDGIFIGPADLAASLGHLGAPSHPEVQAEIEKALAMLKRLGVPSGILAADETLARKFLAAGATFVAVGIDTM
VLSRALSALAATYKDVAPPAQTDRAGY

Sequences:

>Translated_267_residues
MPTPANPFKRALAEGRPQIGLWVGLANPYTAEICAGAGFDWLLIDGEHAPNDVPGLLAQLQAVAPYPSHPIVRPVIGDTA
LIKQLLDIGAQTLLVPMIDTPEQAAATVAAMHYPPRGVRGVGAALARASRWGRIGDYLKTASDELCLLLQIETVTGLRNL
EAIATTDGVDGIFIGPADLAASLGHLGAPSHPEVQAEIEKALAMLKRLGVPSGILAADETLARKFLAAGATFVAVGIDTM
VLSRALSALAATYKDVAPPAQTDRAGY
>Mature_266_residues
PTPANPFKRALAEGRPQIGLWVGLANPYTAEICAGAGFDWLLIDGEHAPNDVPGLLAQLQAVAPYPSHPIVRPVIGDTAL
IKQLLDIGAQTLLVPMIDTPEQAAATVAAMHYPPRGVRGVGAALARASRWGRIGDYLKTASDELCLLLQIETVTGLRNLE
AIATTDGVDGIFIGPADLAASLGHLGAPSHPEVQAEIEKALAMLKRLGVPSGILAADETLARKFLAAGATFVAVGIDTMV
LSRALSALAATYKDVAPPAQTDRAGY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=250, Percent_Identity=55.6, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=48.1927710843374, Blast_Score=238, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27803; Mature: 27672

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPANPFKRALAEGRPQIGLWVGLANPYTAEICAGAGFDWLLIDGEHAPNDVPGLLAQL
CCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
QAVAPYPSHPIVRPVIGDTALIKQLLDIGAQTLLVPMIDTPEQAAATVAAMHYPPRGVRG
HHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHH
VGAALARASRWGRIGDYLKTASDELCLLLQIETVTGLRNLEAIATTDGVDGIFIGPADLA
HHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEHHHHCHHHHHHHEECCCCCEEEECHHHHH
ASLGHLGAPSHPEVQAEIEKALAMLKRLGVPSGILAADETLARKFLAAGATFVAVGIDTM
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHH
VLSRALSALAATYKDVAPPAQTDRAGY
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PTPANPFKRALAEGRPQIGLWVGLANPYTAEICAGAGFDWLLIDGEHAPNDVPGLLAQL
CCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
QAVAPYPSHPIVRPVIGDTALIKQLLDIGAQTLLVPMIDTPEQAAATVAAMHYPPRGVRG
HHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHH
VGAALARASRWGRIGDYLKTASDELCLLLQIETVTGLRNLEAIATTDGVDGIFIGPADLA
HHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEHHHHCHHHHHHHEECCCCCEEEECHHHHH
ASLGHLGAPSHPEVQAEIEKALAMLKRLGVPSGILAADETLARKFLAAGATFVAVGIDTM
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHH
VLSRALSALAATYKDVAPPAQTDRAGY
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA