Click here to switch to the map view.

The map label for this gene is ispH [H]

Identifier: 83309863

GI number: 83309863

Start: 820738

End: 821694

Strand: Reverse

Name: ispH [H]

Synonym: amb0764

Alternate gene names: 83309863

Gene position: 821694-820738 (Counterclockwise)

Preceding gene: 83309872

Following gene: 83309862

Centisome position: 16.54

GC content: 68.76

Gene sequence:

>957_bases
GTGAGCACAAGCGTTACCAAGAGGCCCCTGACCCTGGTTCTGGCCAGCCCGCGCGGATTTTGCGCCGGCGTCGATCGCGC
CATCCACATCGTCGAGCGGGCTCTCGAGAAATACGGCGCTCCGGTCTATGTCCGCCACGAGATCGTCCACAACCGGTTCG
TGGTGGAATCCCTGGCCCAGAAGGGCGCGGTGTTCGTCGAGGAGCTGAACGAGGTCCCCGACGAGGCCGCCGTGGTGTTC
TCCGCCCATGGCGTGCCCAAGACGGTGCCAGCCGAGGCCGACCGCCGGGGCCTGACCTCGCTGGACGCCACCTGTCCGCT
GGTGACCAAGGTTCACCGCGAGGCCGAGCTGCACCACGCCATGGGCCGCCAGATCATCATGATCGGCCATGCCGGCCACC
CCGAGGTGATCGGCACCATCGGCCAGGTTCCCGAAGGGACCGTGCTGCTGGTCGGCACGCCGGAGCAGGCCGAAACCGTC
GCCCCCAGGGACCCGTCCATGCTGGCCTACATCACCCAGACCACGCTGTCGGTGGACGACGCGGCGGCGGTGATCGACGT
GCTGCAGCGCCGCTTCCCCGACATGGTCGGCCCCAAGCGCGAGGACATCTGCTACGCCACCACCAACCGCCAGGCCGCGG
TGAAAACCATCGCCCCCCATTGCGAGGCGCTGATGGTCATCGGCTCGCCCAATTCCTCCAACTCGTCGCGTCTGGTGGAA
GTGGCGGCGCGGGCGGGCTGCGCCCGCTCGGTACTGGTCCAGCGCGCCGCCGAGGTGGATTGGAGCCTGCTGGACGGCGT
CGACCGCCTCGGCATCACCGCCGGCGCCTCGGCCCCGGAGATCCTGGTGGAGGAAGTGGTGGCGGCGGCGCGGGAACGCT
TCGACGTCACCATCGAGGAAATCCGCGTCACCACCGAAACGGTCACCTTCAAGCTGCCCCAGGCCCTGTCGGAATAG

Upstream 100 bases:

>100_bases
AGCCACATGATCTGATAAAGGTGCCCCTGACTGTAGTGAAGCCTGGGCGCCGATACAAGCCGCCAACCCCGTACCCCCCG
CCATTCCGGAGAGCGACACC

Downstream 100 bases:

>100_bases
GCCCCGGCAGGGTTTGTCATCCCGAGCGCATGCGAGGGATCTCCGTCTGGCGCGACGATACCGATCCTGAAAGGCCGCGT
CAGAACGAGATCCCTCCTCC

Product: penicillin tolerance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSTSVTKRPLTLVLASPRGFCAGVDRAIHIVERALEKYGAPVYVRHEIVHNRFVVESLAQKGAVFVEELNEVPDEAAVVF
SAHGVPKTVPAEADRRGLTSLDATCPLVTKVHREAELHHAMGRQIIMIGHAGHPEVIGTIGQVPEGTVLLVGTPEQAETV
APRDPSMLAYITQTTLSVDDAAAVIDVLQRRFPDMVGPKREDICYATTNRQAAVKTIAPHCEALMVIGSPNSSNSSRLVE
VAARAGCARSVLVQRAAEVDWSLLDGVDRLGITAGASAPEILVEEVVAAARERFDVTIEEIRVTTETVTFKLPQALSE

Sequences:

>Translated_318_residues
MSTSVTKRPLTLVLASPRGFCAGVDRAIHIVERALEKYGAPVYVRHEIVHNRFVVESLAQKGAVFVEELNEVPDEAAVVF
SAHGVPKTVPAEADRRGLTSLDATCPLVTKVHREAELHHAMGRQIIMIGHAGHPEVIGTIGQVPEGTVLLVGTPEQAETV
APRDPSMLAYITQTTLSVDDAAAVIDVLQRRFPDMVGPKREDICYATTNRQAAVKTIAPHCEALMVIGSPNSSNSSRLVE
VAARAGCARSVLVQRAAEVDWSLLDGVDRLGITAGASAPEILVEEVVAAARERFDVTIEEIRVTTETVTFKLPQALSE
>Mature_317_residues
STSVTKRPLTLVLASPRGFCAGVDRAIHIVERALEKYGAPVYVRHEIVHNRFVVESLAQKGAVFVEELNEVPDEAAVVFS
AHGVPKTVPAEADRRGLTSLDATCPLVTKVHREAELHHAMGRQIIMIGHAGHPEVIGTIGQVPEGTVLLVGTPEQAETVA
PRDPSMLAYITQTTLSVDDAAAVIDVLQRRFPDMVGPKREDICYATTNRQAAVKTIAPHCEALMVIGSPNSSNSSRLVEV
AARAGCARSVLVQRAAEVDWSLLDGVDRLGITAGASAPEILVEEVVAAARERFDVTIEEIRVTTETVTFKLPQALSE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=51.7799352750809, Blast_Score=322, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34186; Mature: 34054

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVTKRPLTLVLASPRGFCAGVDRAIHIVERALEKYGAPVYVRHEIVHNRFVVESLAQ
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
KGAVFVEELNEVPDEAAVVFSAHGVPKTVPAEADRRGLTSLDATCPLVTKVHREAELHHA
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MGRQIIMIGHAGHPEVIGTIGQVPEGTVLLVGTPEQAETVAPRDPSMLAYITQTTLSVDD
CCCEEEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCHH
AAAVIDVLQRRFPDMVGPKREDICYATTNRQAAVKTIAPHCEALMVIGSPNSSNSSRLVE
HHHHHHHHHHHCCHHCCCCCCCEEEEECCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHH
VAARAGCARSVLVQRAAEVDWSLLDGVDRLGITAGASAPEILVEEVVAAARERFDVTIEE
HHHHHCHHHHHHHHHHHHCCHHHHCCHHHCCEECCCCCHHHHHHHHHHHHHHHCCCCHHH
IRVTTETVTFKLPQALSE
EEEEHHHEEEECCHHHCC
>Mature Secondary Structure 
STSVTKRPLTLVLASPRGFCAGVDRAIHIVERALEKYGAPVYVRHEIVHNRFVVESLAQ
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
KGAVFVEELNEVPDEAAVVFSAHGVPKTVPAEADRRGLTSLDATCPLVTKVHREAELHHA
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MGRQIIMIGHAGHPEVIGTIGQVPEGTVLLVGTPEQAETVAPRDPSMLAYITQTTLSVDD
CCCEEEEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCHH
AAAVIDVLQRRFPDMVGPKREDICYATTNRQAAVKTIAPHCEALMVIGSPNSSNSSRLVE
HHHHHHHHHHHCCHHCCCCCCCEEEEECCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHH
VAARAGCARSVLVQRAAEVDWSLLDGVDRLGITAGASAPEILVEEVVAAARERFDVTIEE
HHHHHCHHHHHHHHHHHHCCHHHHCCHHHCCEECCCCCHHHHHHHHHHHHHHHCCCCHHH
IRVTTETVTFKLPQALSE
EEEEHHHEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA