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The map label for this gene is caiD [H]

Identifier: 83309809

GI number: 83309809

Start: 769361

End: 770164

Strand: Reverse

Name: caiD [H]

Synonym: amb0710

Alternate gene names: 83309809

Gene position: 770164-769361 (Counterclockwise)

Preceding gene: 83309812

Following gene: 83309808

Centisome position: 15.51

GC content: 69.9

Gene sequence:

>804_bases
TTGTCCCGGGGAGGGGAAAGCATGACCGACCATATCCATGTCAAGATCAGCGGCGCCGTCGCCACCGTGACGCTGCGCCG
TCCCGAGTTGCACAACGCCCTGGACGAGCAGATGGTGGGCAACCTCGCCCAGACCTTCCAGAAGCTGTCCGTGGCCGAGG
CGGTGCGCGTGGTGGTGATCGAGGGCCAGGGCCCCAGCTTCTGCGCCGGCGGCGACATCGGCTGGACGCGGGCCATGCTG
GACCAGGACGCGGAAGAGGTCAGCCGCAGCGCCATGCAGATGGCGGTGATGCTGGACGCCATCGACCGCTGCGCCAAGCC
GGTGATCGCCAGGGTCCAGGGCGCCGCCCTGGGGATGGGCGCCGGCATCGTCTCGGTGGCCGACATGGTGGTGGCCGCCG
ACGATTCCAGCTTCTCCCTGCCCGAGGTGCGGGCCGGCTTCCCACCTACCCTGATCATGCCCTATCTGGCCGCCGCCATG
GGCACCCGGGCCATGCGCCGCTACGTGCTGTCGGGCGAGCGCTTCGACGCCCGCGAGGCGCTGCGCCTGGGCCTGATCCA
TGCGGTGGTGGCCGGCGACAAACTGGACTCCGCCCGCGACCTGATGGTCGAGTCCTGCCTCAAGGGCGCCCCCAAGGCTC
AAGGAGCCGCCAAGGACATGCTGCGGGTGGTCGACGACAGCCCCGCCGGACCCGACCTCATGCGCTACACCGTGGCGCAG
TTCGTCGACGCCCGGGCCGGTGCCGAATGCCGCGAGGGCGTGCTGGCCCTGACCGAAAAGAGAAAGCCGAACTGGAGCGT
CTAG

Upstream 100 bases:

>100_bases
TCAAAGCCGCCCCTGGCAACAGGAAATGGAACGCGTCCACCACAAGCCTGCGCTGCTTGCATCTTGGTTGATCTGGCGAT
GCCGTGGTTTACTGGGGCAA

Downstream 100 bases:

>100_bases
GGAAACCGGTCATTCCGACGACCATCGGAAGGAGGAATCTCCGTCTGCTCCGCCGTTTCCTAACCGGACATGCCGGGCCA
GGACGAGATCCCTCGCCTTC

Product: enoyl-CoA hydratase/carnithine racemase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSRGGESMTDHIHVKISGAVATVTLRRPELHNALDEQMVGNLAQTFQKLSVAEAVRVVVIEGQGPSFCAGGDIGWTRAML
DQDAEEVSRSAMQMAVMLDAIDRCAKPVIARVQGAALGMGAGIVSVADMVVAADDSSFSLPEVRAGFPPTLIMPYLAAAM
GTRAMRRYVLSGERFDAREALRLGLIHAVVAGDKLDSARDLMVESCLKGAPKAQGAAKDMLRVVDDSPAGPDLMRYTVAQ
FVDARAGAECREGVLALTEKRKPNWSV

Sequences:

>Translated_267_residues
MSRGGESMTDHIHVKISGAVATVTLRRPELHNALDEQMVGNLAQTFQKLSVAEAVRVVVIEGQGPSFCAGGDIGWTRAML
DQDAEEVSRSAMQMAVMLDAIDRCAKPVIARVQGAALGMGAGIVSVADMVVAADDSSFSLPEVRAGFPPTLIMPYLAAAM
GTRAMRRYVLSGERFDAREALRLGLIHAVVAGDKLDSARDLMVESCLKGAPKAQGAAKDMLRVVDDSPAGPDLMRYTVAQ
FVDARAGAECREGVLALTEKRKPNWSV
>Mature_266_residues
SRGGESMTDHIHVKISGAVATVTLRRPELHNALDEQMVGNLAQTFQKLSVAEAVRVVVIEGQGPSFCAGGDIGWTRAMLD
QDAEEVSRSAMQMAVMLDAIDRCAKPVIARVQGAALGMGAGIVSVADMVVAADDSSFSLPEVRAGFPPTLIMPYLAAAMG
TRAMRRYVLSGERFDAREALRLGLIHAVVAGDKLDSARDLMVESCLKGAPKAQGAAKDMLRVVDDSPAGPDLMRYTVAQF
VDARAGAECREGVLALTEKRKPNWSV

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=26.5625, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI37594471, Length=180, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=26.0416666666667, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=28.4210526315789, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI70995211, Length=255, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=30.2238805970149, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=30.9803921568627, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=25.7575757575758, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=24.1509433962264, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=27.8195488721804, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=29.296875, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=200, Percent_Identity=29, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=202, Percent_Identity=27.7227722772277, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=23.828125, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=28.2258064516129, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=183, Percent_Identity=28.9617486338798, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=27.4611398963731, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129971, Length=282, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653477, Length=282, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=178, Percent_Identity=29.7752808988764, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=27.0676691729323, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24654903, Length=191, Percent_Identity=23.5602094240838, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28426; Mature: 28295

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGGESMTDHIHVKISGAVATVTLRRPELHNALDEQMVGNLAQTFQKLSVAEAVRVVVI
CCCCCCCCCCEEEEEEECEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
EGQGPSFCAGGDIGWTRAMLDQDAEEVSRSAMQMAVMLDAIDRCAKPVIARVQGAALGMG
ECCCCCEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGIVSVADMVVAADDSSFSLPEVRAGFPPTLIMPYLAAAMGTRAMRRYVLSGERFDAREA
HHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LRLGLIHAVVAGDKLDSARDLMVESCLKGAPKAQGAAKDMLRVVDDSPAGPDLMRYTVAQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
FVDARAGAECREGVLALTEKRKPNWSV
HHHHHCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRGGESMTDHIHVKISGAVATVTLRRPELHNALDEQMVGNLAQTFQKLSVAEAVRVVVI
CCCCCCCCCEEEEEEECEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
EGQGPSFCAGGDIGWTRAMLDQDAEEVSRSAMQMAVMLDAIDRCAKPVIARVQGAALGMG
ECCCCCEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGIVSVADMVVAADDSSFSLPEVRAGFPPTLIMPYLAAAMGTRAMRRYVLSGERFDAREA
HHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LRLGLIHAVVAGDKLDSARDLMVESCLKGAPKAQGAAKDMLRVVDDSPAGPDLMRYTVAQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
FVDARAGAECREGVLALTEKRKPNWSV
HHHHHCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA