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The map label for this gene is higB-1 [H]

Identifier: 83309760

GI number: 83309760

Start: 712798

End: 713076

Strand: Reverse

Name: higB-1 [H]

Synonym: amb0661

Alternate gene names: 83309760

Gene position: 713076-712798 (Counterclockwise)

Preceding gene: 83309761

Following gene: 83309759

Centisome position: 14.36

GC content: 58.42

Gene sequence:

>279_bases
ATGATAGCCAGCTTTCACGACAAGGAAACGGAGCGCCTATATCAGGCGGGCACGTCCAAGCGGTTTGCCGCTATCGCCAA
AGCCGCGTTACGCAAGCTGGACATGCTTGATGCGGCTGCCGAGTTGCAAGACCTCTCCTCACCCCCAGGGAACCGCCTTG
AGGCTCTGGAGGGCGACAGGAAGGGCCAACACAGCATCCGTATCAATGACCAGTGGCGGCTGTGTTTTATATGGCGTGAT
GGTGCCGCGCACCGTGTCGAGATCACCGACTACCATTGA

Upstream 100 bases:

>100_bases
TGGGCCGTATGTGGGCCGTTTTTGATTTCCAGAGCGCGACGCAAGCCGCGCAGCCAATGCACCGATTGACGTTATACGTG
GCGCGTTATATCGTTGTCAC

Downstream 100 bases:

>100_bases
GGCAGGATCATGCGCATCAAGACCCATCCCGGTGAAATCCTCCGCGAAGAATTCATGGCCCCCTTGGGGCTGTCGGCCAA
CGCCCTTGCCACGGCGTTAC

Product: plasmid maintenance system killer protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MIASFHDKETERLYQAGTSKRFAAIAKAALRKLDMLDAAAELQDLSSPPGNRLEALEGDRKGQHSIRINDQWRLCFIWRD
GAAHRVEITDYH

Sequences:

>Translated_92_residues
MIASFHDKETERLYQAGTSKRFAAIAKAALRKLDMLDAAAELQDLSSPPGNRLEALEGDRKGQHSIRINDQWRLCFIWRD
GAAHRVEITDYH
>Mature_92_residues
MIASFHDKETERLYQAGTSKRFAAIAKAALRKLDMLDAAAELQDLSSPPGNRLEALEGDRKGQHSIRINDQWRLCFIWRD
GAAHRVEITDYH

Specific function: Inhibits translation by cleavage of mRNA [H]

COG id: COG3549

COG function: function code R; Plasmid maintenance system killer protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007711 [H]

Pfam domain/function: PF05015 Plasmid_killer [H]

EC number: NA

Molecular weight: Translated: 10494; Mature: 10494

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASFHDKETERLYQAGTSKRFAAIAKAALRKLDMLDAAAELQDLSSPPGNRLEALEGDR
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KGQHSIRINDQWRLCFIWRDGAAHRVEITDYH
CCCCEEEECCCEEEEEEECCCCCEEEEEECCC
>Mature Secondary Structure
MIASFHDKETERLYQAGTSKRFAAIAKAALRKLDMLDAAAELQDLSSPPGNRLEALEGDR
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KGQHSIRINDQWRLCFIWRDGAAHRVEITDYH
CCCCEEEECCCEEEEEEECCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]