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The map label for this gene is yafC [H]

Identifier: 83309761

GI number: 83309761

Start: 713495

End: 714388

Strand: Reverse

Name: yafC [H]

Synonym: amb0662

Alternate gene names: 83309761

Gene position: 714388-713495 (Counterclockwise)

Preceding gene: 83309762

Following gene: 83309760

Centisome position: 14.38

GC content: 64.43

Gene sequence:

>894_bases
ATGGACTGGGATAAGCTGAGGGTTTTTCATGCCGTCGCCGAGGCGGGCAGCTTCACCCATGCGGGCGAGGTGCTCAATCT
CAGCCAATCGGCGGTCAGCCGCCAGATCAGTGCGCTGGAGGAAAGCCTTAACCTGCCGCTGTTTCACCGCCATGCCCGCG
GCCTGATCCTCACCGAGCAGGGCGAACTGCTCTACCGCACCGCGCGCGACGTGTTCTCCAAGCTGGCCATGACCGAGGCC
CTGCTGACCGAAAGCCGTGAACGCGCCCAGGGACCGCTGAAGATCACCACCACCGTGGCCTTCGGCTCGCTGTGGCTGAC
GCCGCGCATCAAGGACTTCCTGGACGCCTACCCCGAGATCGCGGTCACCATGGTGCTGTTCGACGGCGAGCTGGACCTGG
CCATGCGCGAGGCCGACGTGGCCATCCGCATGATGCCGCCGCGCCAGCCCGACCTGATCCAGCGGCACCTTTTGTCCATG
AACTACGCCGTCTTCGCCGCGCCCTCGTATATCGAAAAATACGGAATGCCGAAGTCCGCCGACGACCTCGACAACCACCG
CATCATCGTCTATGGCGACGACACCCGGGTCTCGGCCCCGGTGTCCAACGTCAACTGGCTGCTGGAAGCCGGCGCCTCCC
CCGACCGGCCGCGCAAGCCGGTGCTGGAGGTCAACAACATCTACGGCATCTACCGCGCCGTCAAAAGCGGACTGGGCATC
GCCGCCATGCCCGAATACCTGCGCGGCGAGGCCGAGGCGCTGGTGCATATCCTTCCCGAGCTGAAGGGCCCCAAGGTCGA
GACGTATTTCGTCTATCCCGAGGAATTGCGCCATTCCAAGCGCATCACGGTCTTCCGCGACTTCCTGCTCTCCAAGATCG
CCGAATCCGCCTGA

Upstream 100 bases:

>100_bases
CACCGCGCATGGACACACGGGTTAAGTTGCGGCACAGTAGCCGGTATGAAATGGGAGGGCGGAAACCATAATCCGCCGCG
GGGAGGAGACCGGTCGCCAC

Downstream 100 bases:

>100_bases
TCCGGTAAAAACCCTACCCGAAAGCATAAGGCATGCGCCAACCGCATGCCTCTATCCTGCTTTTTGGTGTGGTTTGAGGC
CGCTCCATGCCGTACATCAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDWDKLRVFHAVAEAGSFTHAGEVLNLSQSAVSRQISALEESLNLPLFHRHARGLILTEQGELLYRTARDVFSKLAMTEA
LLTESRERAQGPLKITTTVAFGSLWLTPRIKDFLDAYPEIAVTMVLFDGELDLAMREADVAIRMMPPRQPDLIQRHLLSM
NYAVFAAPSYIEKYGMPKSADDLDNHRIIVYGDDTRVSAPVSNVNWLLEAGASPDRPRKPVLEVNNIYGIYRAVKSGLGI
AAMPEYLRGEAEALVHILPELKGPKVETYFVYPEELRHSKRITVFRDFLLSKIAESA

Sequences:

>Translated_297_residues
MDWDKLRVFHAVAEAGSFTHAGEVLNLSQSAVSRQISALEESLNLPLFHRHARGLILTEQGELLYRTARDVFSKLAMTEA
LLTESRERAQGPLKITTTVAFGSLWLTPRIKDFLDAYPEIAVTMVLFDGELDLAMREADVAIRMMPPRQPDLIQRHLLSM
NYAVFAAPSYIEKYGMPKSADDLDNHRIIVYGDDTRVSAPVSNVNWLLEAGASPDRPRKPVLEVNNIYGIYRAVKSGLGI
AAMPEYLRGEAEALVHILPELKGPKVETYFVYPEELRHSKRITVFRDFLLSKIAESA
>Mature_297_residues
MDWDKLRVFHAVAEAGSFTHAGEVLNLSQSAVSRQISALEESLNLPLFHRHARGLILTEQGELLYRTARDVFSKLAMTEA
LLTESRERAQGPLKITTTVAFGSLWLTPRIKDFLDAYPEIAVTMVLFDGELDLAMREADVAIRMMPPRQPDLIQRHLLSM
NYAVFAAPSYIEKYGMPKSADDLDNHRIIVYGDDTRVSAPVSNVNWLLEAGASPDRPRKPVLEVNNIYGIYRAVKSGLGI
AAMPEYLRGEAEALVHILPELKGPKVETYFVYPEELRHSKRITVFRDFLLSKIAESA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=32.312925170068, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=29.9003322259136, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=28.8659793814433, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=28.3783783783784, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787128, Length=192, Percent_Identity=31.25, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=27.6094276094276, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789173, Length=186, Percent_Identity=34.9462365591398, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=28.4697508896797, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=29.1304347826087, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=26.7441860465116, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786448, Length=115, Percent_Identity=37.3913043478261, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788481, Length=246, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=173, Percent_Identity=27.1676300578035, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33381; Mature: 33381

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWDKLRVFHAVAEAGSFTHAGEVLNLSQSAVSRQISALEESLNLPLFHRHARGLILTEQ
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECC
GELLYRTARDVFSKLAMTEALLTESRERAQGPLKITTTVAFGSLWLTPRIKDFLDAYPEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEECCHHHHHHHCCCCC
AVTMVLFDGELDLAMREADVAIRMMPPRQPDLIQRHLLSMNYAVFAAPSYIEKYGMPKSA
EEEEEEECCCCCEEEHHCCEEEEECCCCCHHHHHHHHHHCCHHEEECHHHHHHCCCCCCC
DDLDNHRIIVYGDDTRVSAPVSNVNWLLEAGASPDRPRKPVLEVNNIYGIYRAVKSGLGI
CCCCCCEEEEEECCCEEECCCCHHHHHEECCCCCCCCCCCEEEHHHHHHHHHHHHHCCCC
AAMPEYLRGEAEALVHILPELKGPKVETYFVYPEELRHSKRITVFRDFLLSKIAESA
CCCHHHHCCHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDWDKLRVFHAVAEAGSFTHAGEVLNLSQSAVSRQISALEESLNLPLFHRHARGLILTEQ
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECC
GELLYRTARDVFSKLAMTEALLTESRERAQGPLKITTTVAFGSLWLTPRIKDFLDAYPEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEECCHHHHHHHCCCCC
AVTMVLFDGELDLAMREADVAIRMMPPRQPDLIQRHLLSMNYAVFAAPSYIEKYGMPKSA
EEEEEEECCCCCEEEHHCCEEEEECCCCCHHHHHHHHHHCCHHEEECHHHHHHCCCCCCC
DDLDNHRIIVYGDDTRVSAPVSNVNWLLEAGASPDRPRKPVLEVNNIYGIYRAVKSGLGI
CCCCCCEEEEEECCCEEECCCCHHHHHEECCCCCCCCCCCEEEHHHHHHHHHHHHHCCCC
AAMPEYLRGEAEALVHILPELKGPKVETYFVYPEELRHSKRITVFRDFLLSKIAESA
CCCHHHHCCHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]