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The map label for this gene is pbpG [H]

Identifier: 83309623

GI number: 83309623

Start: 565137

End: 567263

Strand: Reverse

Name: pbpG [H]

Synonym: amb0524

Alternate gene names: 83309623

Gene position: 567263-565137 (Counterclockwise)

Preceding gene: 83309625

Following gene: 83309621

Centisome position: 11.42

GC content: 68.74

Gene sequence:

>2127_bases
GTGGTAGAAGGCCCCATGGCAGTTCCTCCCTCGCGCCCCAAGGGTGCCGGCAATCCGTTCAAGCTCTCCATCGACCCGGC
CGACCGGCTGTTGCCCAGCGGTTCCGGGGGCGAACCCCCGCCCCCGCGTGAGCCGCCGCCGCCGCGTGGCCGCGCCCCCA
AGGAGCCAAAAAAGCCCAAGGCGCCCAAGAAGGGCGGCTCGGGCAATGGCCGCAAATGGGGCAAGCGCCTGCTGGTCTGG
GGCCTGACCCTGGCCATCTGGATCGGCATCGGCCTGGCCGGCGTGGTGGCTTATTACGCCCTGGACCTGCCCGACATCGA
CCGCATGACCGCCCAGACCAGGCGCCCCAGTGTGGTGTTCCAGTCGGTGGAGGGCGAGATCTTCGCCGCCTATGGCGACC
TCTACGGCGAGCCGCTGGACCTGGGCGAGATGTCGCCCTTCATCGCCCAGGCGGTGCTGGCCACCGAGGACCGGCGCTTC
TACAGCCATTGGGGCGTCGACCCCATCGGCCTTGCCCGCGCCCTATTCGTGAACCTGAGGGCCGGGCACACGGTGCAAGG
CGGCTCGACCATCACCCAGCAGCTGGCCAAGAACCTGTTCCTGACGCCTGACCGCAACATGAAGCGCAAGGTGCAGGAAG
TGCTGATGGCGCTGTGGCTGGAAAAGCGCTTCTCCAAGGAGCAGATCCTGGGGCTTTACCTCAACCGGGTCTATCTCGGG
TCCGGCACCTTCGGCGTGGACGCCGCCGCCAAGCGCTATTTCGACGTCTCGGCCCGCAAGGTCGACGTCTATCAGGCCGC
CGTGCTGGCCGGCTTGCTGAAGGCTCCCTCGCGCTATTCGCCGCTCAACGACCCCGAGGCCTCGCGCAAGCGCACCAGCG
ACGTACTGGCCAACATGGTCAAGGCCGGCTACATCGACCAGAAGACCGCCGACAATGTCCAGGTGACCGGCGCCGCCCAG
CTGGTCCGCCGCCCCATTCCCGCCGGCCGCTATTTCGCCGACTGGCTGATGGCTAATCTCGACCAGTTCGGCGAAGTGGC
CGGCAAGGACATCGTGGTGCACACCACCATGGACATCGCGCTCCAGCGCAAGGTCGAGGCCGACCTCAAGACCATGATGG
CCGGGCCCGGCGCCAAGGCCAACGCCTCCCAGGGCGCCGTGGTGGTGATGAGCCCCGACGGCGCCATCCGGGCGCTGACC
GGCGGCAAGGATTACGACGACAGCCAGTTCAACCGCGCCACCCAGGGGCTGCGCCAGCCCGGCTCGTCGTTCAAGCCCTT
CGTCTATCTCACCGCCATGGAAATGGGCCGCACCCCCGACGACGAGGTGGAGGACAAGCCCATCCGCCTGGGCAACTGGA
GCCCCGGCAATTACACCGGCAAGTATCTCGGCCCCATCACCCTGCGCCAGGCCCTGGCGGAATCGGTCAACACCGTCGCC
GTGCGGCTGGTGGAGGAAGTGGGTCCCGGCCGGGTCATCGCCACGGCCCGCCGGCTGGGCATCACCTCGGACCTGCGCAA
CGACGCCACCCTGGCCCTGGGCACCAGCGAGGTCTCGCTGCTGGAGCTGACCACCGCCTATGCCGCCCTGGCCAATGGCG
GCTATGGGGTGACGTCCTTCGGCGTCTCCCACATCACCGACCCATCCGGCAAAGTGCTGTATCAGCGCCAGGGCGGCGGC
TTCGGCCAGGTGGTGTCACCGGCGGCGCTGTCGCGCATGCACGACATGATGAGCGCCGTCGTCACCCAGGGCTCGGGCAA
GGCCGCCCGCCTGGACCGCCCGGTGGCGGGCAAGACCGGCACCACCCAGGATTACCGCGACGCCTGGTTCATGGGCTTCA
CCGCCGATTACGTGGCCGGCGTGTGGATGGGCAACGACGATTACCGCATCGAGATGAAGAAGGTCACCGGCGGCGGCCTG
CCCGCCCAATTGTGGAAGCAGGTGATGACCGCCGCCCATCGCGGCCTGCCTGCCCGGCCGCTGCGCACCCCGGAGGTTCC
GGCGGAATCCTCCCCAGCCGAAAGCGTCGGCGACTTCGTGGCCGGGGCCGCCCAAGCGGCGGGGGACGCGGCCAAGGGCA
TCGGCGGCGCCATCGACGACCTGCTGAAGGGGATCTTCGGACGGTAA

Upstream 100 bases:

>100_bases
GCAATCGACAGGGTTTCCATGGTTCGGTCCTCGTTAAACATTCAGCCGGGACTCTACATCCAATGAACGGATGTTGCCAG
TTCCGAGCAGCCCCCACCCC

Downstream 100 bases:

>100_bases
TACCAAATCCCGATGAGTTCGGCTTATGGGGATTTGGTTAGGCCCAAGGGGCCGCGCCGCTTATGCGGCGGAAGGCAAGG
ACGCACAGCGTCCGCCCGCC

Product: membrane carboxypeptidase

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MVEGPMAVPPSRPKGAGNPFKLSIDPADRLLPSGSGGEPPPPREPPPPRGRAPKEPKKPKAPKKGGSGNGRKWGKRLLVW
GLTLAIWIGIGLAGVVAYYALDLPDIDRMTAQTRRPSVVFQSVEGEIFAAYGDLYGEPLDLGEMSPFIAQAVLATEDRRF
YSHWGVDPIGLARALFVNLRAGHTVQGGSTITQQLAKNLFLTPDRNMKRKVQEVLMALWLEKRFSKEQILGLYLNRVYLG
SGTFGVDAAAKRYFDVSARKVDVYQAAVLAGLLKAPSRYSPLNDPEASRKRTSDVLANMVKAGYIDQKTADNVQVTGAAQ
LVRRPIPAGRYFADWLMANLDQFGEVAGKDIVVHTTMDIALQRKVEADLKTMMAGPGAKANASQGAVVVMSPDGAIRALT
GGKDYDDSQFNRATQGLRQPGSSFKPFVYLTAMEMGRTPDDEVEDKPIRLGNWSPGNYTGKYLGPITLRQALAESVNTVA
VRLVEEVGPGRVIATARRLGITSDLRNDATLALGTSEVSLLELTTAYAALANGGYGVTSFGVSHITDPSGKVLYQRQGGG
FGQVVSPAALSRMHDMMSAVVTQGSGKAARLDRPVAGKTGTTQDYRDAWFMGFTADYVAGVWMGNDDYRIEMKKVTGGGL
PAQLWKQVMTAAHRGLPARPLRTPEVPAESSPAESVGDFVAGAAQAAGDAAKGIGGAIDDLLKGIFGR

Sequences:

>Translated_708_residues
MVEGPMAVPPSRPKGAGNPFKLSIDPADRLLPSGSGGEPPPPREPPPPRGRAPKEPKKPKAPKKGGSGNGRKWGKRLLVW
GLTLAIWIGIGLAGVVAYYALDLPDIDRMTAQTRRPSVVFQSVEGEIFAAYGDLYGEPLDLGEMSPFIAQAVLATEDRRF
YSHWGVDPIGLARALFVNLRAGHTVQGGSTITQQLAKNLFLTPDRNMKRKVQEVLMALWLEKRFSKEQILGLYLNRVYLG
SGTFGVDAAAKRYFDVSARKVDVYQAAVLAGLLKAPSRYSPLNDPEASRKRTSDVLANMVKAGYIDQKTADNVQVTGAAQ
LVRRPIPAGRYFADWLMANLDQFGEVAGKDIVVHTTMDIALQRKVEADLKTMMAGPGAKANASQGAVVVMSPDGAIRALT
GGKDYDDSQFNRATQGLRQPGSSFKPFVYLTAMEMGRTPDDEVEDKPIRLGNWSPGNYTGKYLGPITLRQALAESVNTVA
VRLVEEVGPGRVIATARRLGITSDLRNDATLALGTSEVSLLELTTAYAALANGGYGVTSFGVSHITDPSGKVLYQRQGGG
FGQVVSPAALSRMHDMMSAVVTQGSGKAARLDRPVAGKTGTTQDYRDAWFMGFTADYVAGVWMGNDDYRIEMKKVTGGGL
PAQLWKQVMTAAHRGLPARPLRTPEVPAESSPAESVGDFVAGAAQAAGDAAKGIGGAIDDLLKGIFGR
>Mature_708_residues
MVEGPMAVPPSRPKGAGNPFKLSIDPADRLLPSGSGGEPPPPREPPPPRGRAPKEPKKPKAPKKGGSGNGRKWGKRLLVW
GLTLAIWIGIGLAGVVAYYALDLPDIDRMTAQTRRPSVVFQSVEGEIFAAYGDLYGEPLDLGEMSPFIAQAVLATEDRRF
YSHWGVDPIGLARALFVNLRAGHTVQGGSTITQQLAKNLFLTPDRNMKRKVQEVLMALWLEKRFSKEQILGLYLNRVYLG
SGTFGVDAAAKRYFDVSARKVDVYQAAVLAGLLKAPSRYSPLNDPEASRKRTSDVLANMVKAGYIDQKTADNVQVTGAAQ
LVRRPIPAGRYFADWLMANLDQFGEVAGKDIVVHTTMDIALQRKVEADLKTMMAGPGAKANASQGAVVVMSPDGAIRALT
GGKDYDDSQFNRATQGLRQPGSSFKPFVYLTAMEMGRTPDDEVEDKPIRLGNWSPGNYTGKYLGPITLRQALAESVNTVA
VRLVEEVGPGRVIATARRLGITSDLRNDATLALGTSEVSLLELTTAYAALANGGYGVTSFGVSHITDPSGKVLYQRQGGG
FGQVVSPAALSRMHDMMSAVVTQGSGKAARLDRPVAGKTGTTQDYRDAWFMGFTADYVAGVWMGNDDYRIEMKKVTGGGL
PAQLWKQVMTAAHRGLPARPLRTPEVPAESSPAESVGDFVAGAAQAAGDAAKGIGGAIDDLLKGIFGR

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1788867, Length=509, Percent_Identity=31.8271119842829, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1786343, Length=519, Percent_Identity=32.9479768786127, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI87082258, Length=305, Percent_Identity=35.4098360655738, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1789601, Length=180, Percent_Identity=37.7777777777778, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 75800; Mature: 75800

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEGPMAVPPSRPKGAGNPFKLSIDPADRLLPSGSGGEPPPPREPPPPRGRAPKEPKKPK
CCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKKGGSGNGRKWGKRLLVWGLTLAIWIGIGLAGVVAYYALDLPDIDRMTAQTRRPSVVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEE
QSVEGEIFAAYGDLYGEPLDLGEMSPFIAQAVLATEDRRFYSHWGVDPIGLARALFVNLR
EECCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHC
AGHTVQGGSTITQQLAKNLFLTPDRNMKRKVQEVLMALWLEKRFSKEQILGLYLNRVYLG
CCCEECCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEC
SGTFGVDAAAKRYFDVSARKVDVYQAAVLAGLLKAPSRYSPLNDPEASRKRTSDVLANMV
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KAGYIDQKTADNVQVTGAAQLVRRPIPAGRYFADWLMANLDQFGEVAGKDIVVHTTMDIA
HHCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
LQRKVEADLKTMMAGPGAKANASQGAVVVMSPDGAIRALTGGKDYDDSQFNRATQGLRQP
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCC
GSSFKPFVYLTAMEMGRTPDDEVEDKPIRLGNWSPGNYTGKYLGPITLRQALAESVNTVA
CCCCCCEEEEEEHHHCCCCCCCCCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHH
VRLVEEVGPGRVIATARRLGITSDLRNDATLALGTSEVSLLELTTAYAALANGGYGVTSF
HHHHHHCCCCCEEHHHHHHCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHC
GVSHITDPSGKVLYQRQGGGFGQVVSPAALSRMHDMMSAVVTQGSGKAARLDRPVAGKTG
CHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCC
TTQDYRDAWFMGFTADYVAGVWMGNDDYRIEMKKVTGGGLPAQLWKQVMTAAHRGLPARP
CCCHHHHHHEECCHHHHHEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
LRTPEVPAESSPAESVGDFVAGAAQAAGDAAKGIGGAIDDLLKGIFGR
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVEGPMAVPPSRPKGAGNPFKLSIDPADRLLPSGSGGEPPPPREPPPPRGRAPKEPKKPK
CCCCCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKKGGSGNGRKWGKRLLVWGLTLAIWIGIGLAGVVAYYALDLPDIDRMTAQTRRPSVVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEE
QSVEGEIFAAYGDLYGEPLDLGEMSPFIAQAVLATEDRRFYSHWGVDPIGLARALFVNLR
EECCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHC
AGHTVQGGSTITQQLAKNLFLTPDRNMKRKVQEVLMALWLEKRFSKEQILGLYLNRVYLG
CCCEECCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEC
SGTFGVDAAAKRYFDVSARKVDVYQAAVLAGLLKAPSRYSPLNDPEASRKRTSDVLANMV
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KAGYIDQKTADNVQVTGAAQLVRRPIPAGRYFADWLMANLDQFGEVAGKDIVVHTTMDIA
HHCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
LQRKVEADLKTMMAGPGAKANASQGAVVVMSPDGAIRALTGGKDYDDSQFNRATQGLRQP
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCC
GSSFKPFVYLTAMEMGRTPDDEVEDKPIRLGNWSPGNYTGKYLGPITLRQALAESVNTVA
CCCCCCEEEEEEHHHCCCCCCCCCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHH
VRLVEEVGPGRVIATARRLGITSDLRNDATLALGTSEVSLLELTTAYAALANGGYGVTSF
HHHHHHCCCCCEEHHHHHHCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHC
GVSHITDPSGKVLYQRQGGGFGQVVSPAALSRMHDMMSAVVTQGSGKAARLDRPVAGKTG
CHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCC
TTQDYRDAWFMGFTADYVAGVWMGNDDYRIEMKKVTGGGLPAQLWKQVMTAAHRGLPARP
CCCHHHHHHEECCHHHHHEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
LRTPEVPAESSPAESVGDFVAGAAQAAGDAAKGIGGAIDDLLKGIFGR
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]