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The map label for this gene is 83309625

Identifier: 83309625

GI number: 83309625

Start: 567742

End: 569199

Strand: Reverse

Name: 83309625

Synonym: amb0526

Alternate gene names: NA

Gene position: 569199-567742 (Counterclockwise)

Preceding gene: 83309626

Following gene: 83309623

Centisome position: 11.46

GC content: 64.81

Gene sequence:

>1458_bases
GTGCCGTCATCGGCAGGCCTGGGAGAGCCCGTCGTAGCCATCCGAGGAGTTACCCCTGACGTGAAAGCGCTCGATCCTAT
CCTGATTTCCGGTCGCGAGGTCCTGCCCCTGGTCGAGGGCGGCAAGGGGATCAACGTCTCCAATGGCGAGAGTTCTGGCG
CCTGGGCGGCTGCCGGCGGCGTCGCCACCTTCTCCGGAGTCAATGCCGATCTCTATGACGAGAACGGCAACCGCATCGAC
ATGGTCTATCACGGCAAGACCAGGACCGAGAAACACCACGAGCTGATCGCCTACGGCATCAGGGGCGGCATCGCCCAGGC
CCGCATCGCCCACGACATCGCCGGCGGCAATGGCCGCATCCACATGAATGTGCTGTGGGAGATGGGCGGCGCCGAGCAGA
TCCTCGAAGGCGTGCTGGACGGCGCCAAGGGCCTGATCCATGGCGTGACCTGCGGTGCCGGCATGCCCTATCGCGTGGCC
GAGATCGCGGCCCGCTATAACGTCTATTACTATCCCATCGTGTCGTCGGCCCGCGCCTTCCGCGCCCTGTGGAAGCGTGC
CTACCACAAGTACGGCGAATTGCTGGGCGGCGTGGTCTACGAGGACCCCTGGCTGGCCGGCGGCCATAACGGCCTGTCCA
ATTCCGAAGACCCGAGGAAGCCCGAGCCCCCCTATGGCCGCGTCGCCGAGCTGCGCAAGACCATGGTGGAATGCGGCCTG
GGCAACGTGCCGATCTTCATGGCCGGCGGCGTCTGGTGGCTGAAGGAATGGGAGGACTGGATCGGCAACCCCGAACTGGG
TCCCATCGCCTTCCAGTACGGCACCCGTCCGCTGCTGACCCAGGAAAGCCCCATCTCCGACGCCTGGAAGCAGCGCCTGC
TGACGCTGAAGCCCGGCGACGTGCTGCTGCACCATTTCAGCCCCACCGGCTTCTACTCCTCGGCGGTGAAGAACGACTTC
ATCAACGAGCTGTGCCAGCGGCTGGACCACCAGATCCCCTATTCCAGCGAATGCGTCGGCGATCACGAGGCCGAGCTGCC
CATCGGCGCCCGCAAGCGCATCGTCTGGGTCACACCCCACGACCGCGACCGGGCGCTGGGCTGGATGAACGAGGGCTTTA
CCGAGGCGCTGCGCACCCCCGATTCGACCCTGATCTTCGTCACCCCCGACAAGGCCGAGGAAATCCGCGTCGATCAGGTC
AAGTGCATGGGCTGCCTGTCCCAGTGCATGTTCTCCAACTGGGCTCAGAACGAGGCCGGGACCACCGGCAAGAAGGCCGA
CCCGCGCAGCTTCTGTATCCAGAAGACCCTGCAGAACATCGGCCATGGCGGCGACGTGGAAAACGAACTGATGTTCGCCG
GCCACAACGCCTATCGCTTCGGCGCCGATCCCTTCTACAAGAACGGCTTCATCCCGACTGTCAAGCAACTGGTGGACCGC
ATCGCCACCGGCGAATAG

Upstream 100 bases:

>100_bases
TCTTGGGCCACTGGGGCGTCGCCGCCGTCTTCGCCGATTTTCCTCATTCGTCATGAGTCCGTAACGTCACTGTGGCGCGG
CACGACAAAGGGCTATATAT

Downstream 100 bases:

>100_bases
GAATTGTCATCCCGAGCGTAGCCGAGGGATCTCGTCCTGGCACGGCGCGTCCAAATTGGCGGCGACGGAGCAAGCGGAGA
TTCCTCGCGACGCTCGGAAT

Product: 2-nitropropane dioxygenase-like protein

Products: acetone; nitrite

Alternate protein names: 2-Nitropropane Dioxygenase; 2-Nitropropane Dioxygenase Family Oxidoreductase; Oxidoreductase 2-Nitropropane Dioxygenase Family; Oxidoreductase; 2-Nitropropane Dioxygenase-Like Protein; 2-Nitropropane Dioxygenase Family; Flavin-Containing Oxidoreductase; Dioxygenase; 2-Nitropropane Dioxygenase Npd; 2-Nitropropane Dioxygenase Family Protein

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MPSSAGLGEPVVAIRGVTPDVKALDPILISGREVLPLVEGGKGINVSNGESSGAWAAAGGVATFSGVNADLYDENGNRID
MVYHGKTRTEKHHELIAYGIRGGIAQARIAHDIAGGNGRIHMNVLWEMGGAEQILEGVLDGAKGLIHGVTCGAGMPYRVA
EIAARYNVYYYPIVSSARAFRALWKRAYHKYGELLGGVVYEDPWLAGGHNGLSNSEDPRKPEPPYGRVAELRKTMVECGL
GNVPIFMAGGVWWLKEWEDWIGNPELGPIAFQYGTRPLLTQESPISDAWKQRLLTLKPGDVLLHHFSPTGFYSSAVKNDF
INELCQRLDHQIPYSSECVGDHEAELPIGARKRIVWVTPHDRDRALGWMNEGFTEALRTPDSTLIFVTPDKAEEIRVDQV
KCMGCLSQCMFSNWAQNEAGTTGKKADPRSFCIQKTLQNIGHGGDVENELMFAGHNAYRFGADPFYKNGFIPTVKQLVDR
IATGE

Sequences:

>Translated_485_residues
MPSSAGLGEPVVAIRGVTPDVKALDPILISGREVLPLVEGGKGINVSNGESSGAWAAAGGVATFSGVNADLYDENGNRID
MVYHGKTRTEKHHELIAYGIRGGIAQARIAHDIAGGNGRIHMNVLWEMGGAEQILEGVLDGAKGLIHGVTCGAGMPYRVA
EIAARYNVYYYPIVSSARAFRALWKRAYHKYGELLGGVVYEDPWLAGGHNGLSNSEDPRKPEPPYGRVAELRKTMVECGL
GNVPIFMAGGVWWLKEWEDWIGNPELGPIAFQYGTRPLLTQESPISDAWKQRLLTLKPGDVLLHHFSPTGFYSSAVKNDF
INELCQRLDHQIPYSSECVGDHEAELPIGARKRIVWVTPHDRDRALGWMNEGFTEALRTPDSTLIFVTPDKAEEIRVDQV
KCMGCLSQCMFSNWAQNEAGTTGKKADPRSFCIQKTLQNIGHGGDVENELMFAGHNAYRFGADPFYKNGFIPTVKQLVDR
IATGE
>Mature_484_residues
PSSAGLGEPVVAIRGVTPDVKALDPILISGREVLPLVEGGKGINVSNGESSGAWAAAGGVATFSGVNADLYDENGNRIDM
VYHGKTRTEKHHELIAYGIRGGIAQARIAHDIAGGNGRIHMNVLWEMGGAEQILEGVLDGAKGLIHGVTCGAGMPYRVAE
IAARYNVYYYPIVSSARAFRALWKRAYHKYGELLGGVVYEDPWLAGGHNGLSNSEDPRKPEPPYGRVAELRKTMVECGLG
NVPIFMAGGVWWLKEWEDWIGNPELGPIAFQYGTRPLLTQESPISDAWKQRLLTLKPGDVLLHHFSPTGFYSSAVKNDFI
NELCQRLDHQIPYSSECVGDHEAELPIGARKRIVWVTPHDRDRALGWMNEGFTEALRTPDSTLIFVTPDKAEEIRVDQVK
CMGCLSQCMFSNWAQNEAGTTGKKADPRSFCIQKTLQNIGHGGDVENELMFAGHNAYRFGADPFYKNGFIPTVKQLVDRI
ATGE

Specific function: Unknown

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.13.11.32

Molecular weight: Translated: 53148; Mature: 53017

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSAGLGEPVVAIRGVTPDVKALDPILISGREVLPLVEGGKGINVSNGESSGAWAAAGG
CCCCCCCCCCEEEEECCCCCHHHCCCEEECCCEEEEEEECCCCCCCCCCCCCCCEEECCC
VATFSGVNADLYDENGNRIDMVYHGKTRTEKHHELIAYGIRGGIAQARIAHDIAGGNGRI
CEEECCCCCEEECCCCCEEEEEECCCCCCCHHHHHHEEHHCCCHHHHHHHHHHCCCCCEE
HMNVLWEMGGAEQILEGVLDGAKGLIHGVTCGAGMPYRVAEIAARYNVYYYPIVSSARAF
EEEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCEEEEEHHHHHHHH
RALWKRAYHKYGELLGGVVYEDPWLAGGHNGLSNSEDPRKPEPPYGRVAELRKTMVECGL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GNVPIFMAGGVWWLKEWEDWIGNPELGPIAFQYGTRPLLTQESPISDAWKQRLLTLKPGD
CCCCEEEECCEEEHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCEEEECCCC
VLLHHFSPTGFYSSAVKNDFINELCQRLDHQIPYSSECVGDHEAELPIGARKRIVWVTPH
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
DRDRALGWMNEGFTEALRTPDSTLIFVTPDKAEEIRVDQVKCMGCLSQCMFSNWAQNEAG
CCCHHHCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TTGKKADPRSFCIQKTLQNIGHGGDVENELMFAGHNAYRFGADPFYKNGFIPTVKQLVDR
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCHHHHHHHHH
IATGE
HHCCC
>Mature Secondary Structure 
PSSAGLGEPVVAIRGVTPDVKALDPILISGREVLPLVEGGKGINVSNGESSGAWAAAGG
CCCCCCCCCEEEEECCCCCHHHCCCEEECCCEEEEEEECCCCCCCCCCCCCCCEEECCC
VATFSGVNADLYDENGNRIDMVYHGKTRTEKHHELIAYGIRGGIAQARIAHDIAGGNGRI
CEEECCCCCEEECCCCCEEEEEECCCCCCCHHHHHHEEHHCCCHHHHHHHHHHCCCCCEE
HMNVLWEMGGAEQILEGVLDGAKGLIHGVTCGAGMPYRVAEIAARYNVYYYPIVSSARAF
EEEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCEEEEEHHHHHHHH
RALWKRAYHKYGELLGGVVYEDPWLAGGHNGLSNSEDPRKPEPPYGRVAELRKTMVECGL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GNVPIFMAGGVWWLKEWEDWIGNPELGPIAFQYGTRPLLTQESPISDAWKQRLLTLKPGD
CCCCEEEECCEEEHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCEEEECCCC
VLLHHFSPTGFYSSAVKNDFINELCQRLDHQIPYSSECVGDHEAELPIGARKRIVWVTPH
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
DRDRALGWMNEGFTEALRTPDSTLIFVTPDKAEEIRVDQVKCMGCLSQCMFSNWAQNEAG
CCCHHHCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TTGKKADPRSFCIQKTLQNIGHGGDVENELMFAGHNAYRFGADPFYKNGFIPTVKQLVDR
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCHHHHHHHHH
IATGE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-nitropropane; O2

Specific reaction: 2 2-nitropropane + O2 = 2 acetone + 2 nitrite

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA