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The map label for this gene is proS

Identifier: 162319780

GI number: 162319780

Start: 3996146

End: 3997648

Strand: Reverse

Name: proS

Synonym: amb3636

Alternate gene names: 162319780

Gene position: 3997648-3996146 (Counterclockwise)

Preceding gene: 83312738

Following gene: 83312733

Centisome position: 80.48

GC content: 66.53

Gene sequence:

>1503_bases
ATGTCCAAGGCCAAGAAGACCGCTGTCACTCCCACCCGCGAGGAGAACTTCCCCGAATGGTACCAGCAGGTGATCAAGGC
CTCCGACATGGCGGAGAATTCCCCCGTGCGCGGCTGCATGGTGATCAAGCCCTGGGGCTACGGCATCTGGGAGGCCATCC
AGCGCGACCTTGACCGCCGCATCAAGGAGACCGGGCACGAGAACTGCTATTTCCCGCTGTTCATCCCGCTGTCCTTCCTG
GAGAAGGAAGCCGCCCATGTGGAGGGCTTCGCCAAGGAGATGGCGGTGGTCACCCACCACCGGCTGGTGGCCGAGGACGG
CAAGCTGGTGCCGTCGGGCAAGCTGGAGGAGCCGCTGGTGGTGCGCCCCACCTCGGAGACCATCATCGGCGACGCCTTCG
CCCGCTGGATTCAGTCCTACCGCGACCTGCCCATGCTGGTGAACCAGTGGGCCAACGTGGTGCGCTGGGAAATGCGGCCG
CGCATCTTCCTGCGCACCGCCGAATTCCTCTGGCAAGAGGGCCACACCGCCCACGCCACGGCCGAGGAGGCCATGGACGA
AACCTTGAAGATGCTGGAAGTTTACCGCGTCATGGCCGAGGAGGTCCTGGCCATGCCGGTGATCGTCGGCGAGAAGCCGG
CCCACGAGCGCTTCCCCGGCGCCGACAAGACCTATTCCATCGAAGCCATGATGCAGGACGGCAAGGCCCTTCAGGCCGGC
ACATCCCACTTCCTGGGCCAGCATTTCTCCAAGGCCCAGAACATCCGCTACCAGACCGCCCAGGGCGGCGAGGAGTTCTG
CTACACCACCTCGTGGGGCGTCTCCACCCGCCTGATCGGCGGCGTGATCATGAGCCATGCCGACGACAACGGCCTGCGGG
TGCCGCCGCGCATCGCCCCCAAGCAGATCGTCTTCGTGCCCATCACCCGGGCCGACGGCGACGAGGCGCTGATCGAGGAC
TTCCTGGCCCCCATCGTCAAGGACCTGTCCGCCCAGAGCTTCGGCGGCGAGCGTCTGCGGGTGCATGTGGACCGCCGTCC
GCTGGCGCCGCCGGAAAAGCGTTGGGAATGGGTGAAGAAGGGCGCTCCCATCATCTGCGAAGTGGGGCCGCGCGACGTGG
CCGGCGGCTCGGTCGCCATGATCCGCCGCGACGGCGAGCTGAAGGGCCAGATCACCCCCAAGGATGAACTGGTCTCCCGC
GCCGCCGCCATCCTGGAGGACATCCAGAAGACCCTGTTTACCCAGGCCGCCACCGCCCTGAAGGAGCGGACGGTGACGGT
GCGGACCCTGGACGACTTCCTGGCCGTCTTCGCCGACGACACCACCTTCGGGCGCAAGTTCGTCCGCGCCCGGTGGTGCG
GCGATTCCGACACCCTGGCCAAGCTGGACGAATACAGCATCACCATCCGCAACGTGCCCTTCGACCAGGACGGCGAGGCG
GGGACCTGCGTGCTGAGCGGACGTCCGGCGACGCAAGAGGTGATCTTCGCCAAGTCCTACTGA

Upstream 100 bases:

>100_bases
CGTGATACGAAACGCAGGAGCGCGGAACCGCTTCCGGCTGGAAAAAGCGGCCGGGATGGGCTATCGGGATGCGTGCTCAC
ATTTCCGGTGGATGAATCCC

Downstream 100 bases:

>100_bases
TCAGGTTGGCGGCACTGTATTCAGGCAATGCGTGTATGCAATTGCTTGAATGCAGGTCGGGGGGCTGTATAATCCAAGAG
CCGCCTTACCTATAACGGGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSKAKKTAVTPTREENFPEWYQQVIKASDMAENSPVRGCMVIKPWGYGIWEAIQRDLDRRIKETGHENCYFPLFIPLSFL
EKEAAHVEGFAKEMAVVTHHRLVAEDGKLVPSGKLEEPLVVRPTSETIIGDAFARWIQSYRDLPMLVNQWANVVRWEMRP
RIFLRTAEFLWQEGHTAHATAEEAMDETLKMLEVYRVMAEEVLAMPVIVGEKPAHERFPGADKTYSIEAMMQDGKALQAG
TSHFLGQHFSKAQNIRYQTAQGGEEFCYTTSWGVSTRLIGGVIMSHADDNGLRVPPRIAPKQIVFVPITRADGDEALIED
FLAPIVKDLSAQSFGGERLRVHVDRRPLAPPEKRWEWVKKGAPIICEVGPRDVAGGSVAMIRRDGELKGQITPKDELVSR
AAAILEDIQKTLFTQAATALKERTVTVRTLDDFLAVFADDTTFGRKFVRARWCGDSDTLAKLDEYSITIRNVPFDQDGEA
GTCVLSGRPATQEVIFAKSY

Sequences:

>Translated_500_residues
MSKAKKTAVTPTREENFPEWYQQVIKASDMAENSPVRGCMVIKPWGYGIWEAIQRDLDRRIKETGHENCYFPLFIPLSFL
EKEAAHVEGFAKEMAVVTHHRLVAEDGKLVPSGKLEEPLVVRPTSETIIGDAFARWIQSYRDLPMLVNQWANVVRWEMRP
RIFLRTAEFLWQEGHTAHATAEEAMDETLKMLEVYRVMAEEVLAMPVIVGEKPAHERFPGADKTYSIEAMMQDGKALQAG
TSHFLGQHFSKAQNIRYQTAQGGEEFCYTTSWGVSTRLIGGVIMSHADDNGLRVPPRIAPKQIVFVPITRADGDEALIED
FLAPIVKDLSAQSFGGERLRVHVDRRPLAPPEKRWEWVKKGAPIICEVGPRDVAGGSVAMIRRDGELKGQITPKDELVSR
AAAILEDIQKTLFTQAATALKERTVTVRTLDDFLAVFADDTTFGRKFVRARWCGDSDTLAKLDEYSITIRNVPFDQDGEA
GTCVLSGRPATQEVIFAKSY
>Mature_499_residues
SKAKKTAVTPTREENFPEWYQQVIKASDMAENSPVRGCMVIKPWGYGIWEAIQRDLDRRIKETGHENCYFPLFIPLSFLE
KEAAHVEGFAKEMAVVTHHRLVAEDGKLVPSGKLEEPLVVRPTSETIIGDAFARWIQSYRDLPMLVNQWANVVRWEMRPR
IFLRTAEFLWQEGHTAHATAEEAMDETLKMLEVYRVMAEEVLAMPVIVGEKPAHERFPGADKTYSIEAMMQDGKALQAGT
SHFLGQHFSKAQNIRYQTAQGGEEFCYTTSWGVSTRLIGGVIMSHADDNGLRVPPRIAPKQIVFVPITRADGDEALIEDF
LAPIVKDLSAQSFGGERLRVHVDRRPLAPPEKRWEWVKKGAPIICEVGPRDVAGGSVAMIRRDGELKGQITPKDELVSRA
AAILEDIQKTLFTQAATALKERTVTVRTLDDFLAVFADDTTFGRKFVRARWCGDSDTLAKLDEYSITIRNVPFDQDGEAG
TCVLSGRPATQEVIFAKSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=527, Percent_Identity=41.1764705882353, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71991342, Length=455, Percent_Identity=36.7032967032967, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI71991346, Length=371, Percent_Identity=40.9703504043127, Blast_Score=301, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6321808, Length=533, Percent_Identity=36.7729831144465, Blast_Score=331, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24649468, Length=462, Percent_Identity=38.3116883116883, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24649466, Length=462, Percent_Identity=38.3116883116883, Blast_Score=316, Evalue=2e-86,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_MAGSA (Q2W136)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422998.2
- ProteinModelPortal:   Q2W136
- SMR:   Q2W136
- STRING:   Q2W136
- GeneID:   3805198
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3636
- NMPDR:   fig|342108.5.peg.3273
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   EGFAKEM
- PhylomeDB:   Q2W136
- ProtClustDB:   PRK08661
- BioCyc:   MMAG342108:AMB3636-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 56166; Mature: 56035

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAKKTAVTPTREENFPEWYQQVIKASDMAENSPVRGCMVIKPWGYGIWEAIQRDLDRR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
IKETGHENCYFPLFIPLSFLEKEAAHVEGFAKEMAVVTHHRLVAEDGKLVPSGKLEEPLV
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
VRPTSETIIGDAFARWIQSYRDLPMLVNQWANVVRWEMRPRIFLRTAEFLWQEGHTAHAT
ECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
AEEAMDETLKMLEVYRVMAEEVLAMPVIVGEKPAHERFPGADKTYSIEAMMQDGKALQAG
HHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEHHHHHHCCCHHHHH
TSHFLGQHFSKAQNIRYQTAQGGEEFCYTTSWGVSTRLIGGVIMSHADDNGLRVPPRIAP
HHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
KQIVFVPITRADGDEALIEDFLAPIVKDLSAQSFGGERLRVHVDRRPLAPPEKRWEWVKK
CEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHC
GAPIICEVGPRDVAGGSVAMIRRDGELKGQITPKDELVSRAAAILEDIQKTLFTQAATAL
CCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KERTVTVRTLDDFLAVFADDTTFGRKFVRARWCGDSDTLAKLDEYSITIRNVPFDQDGEA
HHCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEEEECCCCCCCCC
GTCVLSGRPATQEVIFAKSY
CEEEECCCCCCCEEEEEECC
>Mature Secondary Structure 
SKAKKTAVTPTREENFPEWYQQVIKASDMAENSPVRGCMVIKPWGYGIWEAIQRDLDRR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
IKETGHENCYFPLFIPLSFLEKEAAHVEGFAKEMAVVTHHRLVAEDGKLVPSGKLEEPLV
HHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
VRPTSETIIGDAFARWIQSYRDLPMLVNQWANVVRWEMRPRIFLRTAEFLWQEGHTAHAT
ECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHH
AEEAMDETLKMLEVYRVMAEEVLAMPVIVGEKPAHERFPGADKTYSIEAMMQDGKALQAG
HHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEHHHHHHCCCHHHHH
TSHFLGQHFSKAQNIRYQTAQGGEEFCYTTSWGVSTRLIGGVIMSHADDNGLRVPPRIAP
HHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
KQIVFVPITRADGDEALIEDFLAPIVKDLSAQSFGGERLRVHVDRRPLAPPEKRWEWVKK
CEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHC
GAPIICEVGPRDVAGGSVAMIRRDGELKGQITPKDELVSRAAAILEDIQKTLFTQAATAL
CCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KERTVTVRTLDDFLAVFADDTTFGRKFVRARWCGDSDTLAKLDEYSITIRNVPFDQDGEA
HHCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEEEECCCCCCCCC
GTCVLSGRPATQEVIFAKSY
CEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA