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The map label for this gene is hisF

Identifier: 82752252

GI number: 82752252

Start: 2705269

End: 2706027

Strand: Reverse

Name: hisF

Synonym: SAB2549c

Alternate gene names: 82752252

Gene position: 2706027-2705269 (Counterclockwise)

Preceding gene: 82752253

Following gene: 82752251

Centisome position: 98.67

GC content: 37.29

Gene sequence:

>759_bases
ATGATTAAAAAACGTATCATTCCATGTTTAGATGTCAAAGATGGTCGTGTCGTTAAAGGGATTCAATTTAAAGGATTAAG
GGATATTGGGAATCCTGTTGATTTAGCAATGTATTACAATGAAGCGGGTGCTGATGAGTTAGTGTTTTTAGACATCTCTA
AGACGGAAGAGGGTCATAGCTTAATGCTAGAAGTGATTGAACAGACAGCGTCACGCTTGTTTATTCCTCTTACTGTAGGA
GGTGGGATTCAAAGTCTCGATGATATTACCCAATTGTTAAATCATGGTGCAGATAAAGTATCATTAAATTCAAGTGCTTT
AAAAAATCCACAGCTCATTAAACAAGCGAGTGATAAATTCGGTAGACAATGCATCTGTATAGCAATTGATAGCTATTATG
ATCCTGAAAGAAAAGCACATTATTGTTGTACGCATGGTGGTAAAAAGATGACAAATATTAAAGTATATGACTGGGTACAG
CAAGTAGAACAGTTAGGCGCAGGTGAGCTCCTCGTTACAAGTATGGGGCATGATGGTATGAAACAAGGATTTGATATTGA
GCATCTTGCAAAAATAAAATCCCTAGTAAATATTCCAATCATTGCTTCTGGTGGTGGTGGCAATGCACAACACTTTGTAG
AATTATTTGATCAGACGGATGTTTCTGCAGGTTTAGCTGCAAGTATATTACATGATCGAGAAACGACGGTTCAATCTATT
AAAGAAGTGATACGGCAAGGGGGTATAGCAGTAAGATGA

Upstream 100 bases:

>100_bases
TTAGACATCAGCAAGATATTCAACGATTAGCGTCGCTAAATGTTCACGCTGCTATTATAGGAAAGGCCGCACATCAAGCA
TCATTTTGGGAGGGGCTTAA

Downstream 100 bases:

>100_bases
CCAATTACAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAATCAAACAAAGCAAGTATTGATGTTG
GGTTATATGAACCAAGAAGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR

Sequences:

>Translated_252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR
>Mature_252_residues
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVG
GGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
KEVIRQGGIAVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=42.578125, Blast_Score=232, Evalue=1e-62,
Organism=Escherichia coli, GI87082028, Length=184, Percent_Identity=27.1739130434783, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=33.2268370607029, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27549; Mature: 27549

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHS
CCCCCCCCCCCCCCCCEEECEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKF
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGM
CCEEEEEEECCCCCCCCCCCEEECCCCCEECEEHHHHHHHHHHHCCCCCEEEEECCCCHH
KQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
HCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHH
KEVIRQGGIAVR
HHHHHHCCCCCC
>Mature Secondary Structure
MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHS
CCCCCCCCCCCCCCCCEEECEEECCHHHCCCCEEEEEEECCCCCCEEEEEEECCCCCHHH
LMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKF
HHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGM
CCEEEEEEECCCCCCCCCCCEEECCCCCEECEEHHHHHHHHHHHCCCCCEEEEECCCCHH
KQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSI
HCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHH
KEVIRQGGIAVR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA