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The map label for this gene is gatA

Identifier: 82751554

GI number: 82751554

Start: 1967534

End: 1968991

Strand: Reverse

Name: gatA

Synonym: SAB1833c

Alternate gene names: 82751554

Gene position: 1968991-1967534 (Counterclockwise)

Preceding gene: 82751555

Following gene: 82751553

Centisome position: 71.79

GC content: 34.02

Gene sequence:

>1458_bases
ATGAGCATTCGCTACGAATCGGTTGAGAATTTATTAACTTTAATAAAAGACAAAAAAATCAAACCATCTGATGTTGTTAA
AGATGTATATGATGCAATTGAAGAGACTGATCCAACAATTAAGTCTTTTCTAGCGCTGGATAAAGAAAATGCAATTAAAA
AAGCGCAAGAATTGGATGAATTACAAGCAAAAGACCAAATGGATGGCAAATTATTTGGTATTCCAATGGGTATAAAAGAT
AACATTATTACAAACGGATTAGAAACAACATGTGCAAGTAAAATGTTAGAAGAATTTGTGCCAATTTACGAATCTACTGT
AATGGAAAAACTACATAATGAGAATGCCGTTTTAATTGGTAAATTAAATATGGATGAGTTTGCAATGGGTGGTTCAACAG
AAACATCTTATTTCAAAAAAACAGTTAACCCATTTGACCATAAAGCAGTACCAGGTGGTTCATCAGGTGGATCTGCAGCA
GCAGTTGCAGCTGGCTTAGTACCATTTAGCTTAGGTTCAGACACAGGTGGTTCAATTAGACAACCGGCTGCATATTGTGG
CGTTGTCGGTATGAAACCAACATACGGTCGTGTATCTCGATTTGGATTAGTTGCTTTTGCATCTTCATTAGACCAAATTG
GTCCATTGACTCGAAATGTAAAAGATAATGCAATCGTATTAGAAGCTATTTCTGGTGCAGATGTTAATGACTCTACAAGT
GCATCTGTTGATGATGTAGACTTTACATCTGAAATTGGTAAAGATATTAAAGGATTAAAAGTTGCATTACCTAAAGAATA
CTTAGGTGAAGGTGTAGCTGATGACGTAAAAGAAGCAGTTCAAAACGCTGTAGAAACTTTAAAATCTTTAGGTGCTGTCG
TTGAGGAAGTATCATTGCCAAATACTAAATTTGGTATTCCATCATATTACGTGATTGCATCATCAGAAGCTTCGTCAAAC
CTTTCTCGTTTTGACGGAATTCGTTATGGTTATCATTCTAAAGAAGCTCATTCATTAGAAGAATTATATAAAATGTCAAG
ATCTGAAGGTTTCGGTAAAGAAGTAAAACGTCGTATTTTCTTAGGTACATTTGCATTAAGTTCAGGTTACTACGATGCTT
ACTATAAAAAATCTCAAAAAGTTAGAACATTGATTAAAAATGACTTTGATAAAGTATTCGAAAATTATGATGTAGTAGTT
GGTCCAACAGCGCCTACAACTGCCTTTAATTTAGGTGAAGAAATTGATGATCCATTAACAATGTATGCCAATGATTTATT
AACAACACCAGTAAACTTAGCTGGATTACCTGGTATTTCTGTTCCTTGTGGACAATCAAATGGCCGACCAATCGGTTTAC
AGTTCATTGGTAAACCATTCGATGAAAAAACGTTATATCGTGTCGCTTATCAATATGAAACACAATACAATTTACATGAC
GTTTATGAAAAATTATAA

Upstream 100 bases:

>100_bases
ATTAAAGGTATTCCGCAAGAATTAGCTTTGAAAAATGCCAAAGAAACAGAAGATGGACAATTTAAAGTGCCTACAATCAT
GAATGAGGAGGACGCGTAAG

Downstream 100 bases:

>100_bases
GGAGTGGAAATCATGCATTTTGAAACAGTTATAGGACTTGAAGTTCACGTAGAGTTAAAAACGGACTCAAAAATGTTTTC
TCCATCACCAGCGCATTTTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSIRYESVENLLTLIKDKKIKPSDVVKDVYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKD
NIITNGLETTCASKMLEEFVPIYESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
ASVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSN
LSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVV
GPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
VYEKL

Sequences:

>Translated_485_residues
MSIRYESVENLLTLIKDKKIKPSDVVKDVYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKD
NIITNGLETTCASKMLEEFVPIYESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
ASVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSN
LSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVV
GPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
VYEKL
>Mature_484_residues
SIRYESVENLLTLIKDKKIKPSDVVKDVYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDN
IITNGLETTCASKMLEEFVPIYESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAA
VAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTSA
SVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNL
SRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVG
PTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHDV
YEKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=38.5858585858586, Blast_Score=322, Evalue=6e-88,
Organism=Homo sapiens, GI195972892, Length=484, Percent_Identity=25.2066115702479, Blast_Score=136, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17543272, Length=429, Percent_Identity=37.995337995338, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=489, Percent_Identity=23.721881390593, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=188, Percent_Identity=34.0425531914894, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=320, Percent_Identity=26.25, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=226, Percent_Identity=29.2035398230088, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=268, Percent_Identity=29.4776119402985, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=268, Percent_Identity=29.4776119402985, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=254, Percent_Identity=27.9527559055118, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=416, Percent_Identity=37.7403846153846, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=451, Percent_Identity=31.2638580931264, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.5918367346939, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=26.2845849802372, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652985, Length=503, Percent_Identity=24.4532803180915, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI19922090, Length=503, Percent_Identity=24.4532803180915, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24652981, Length=503, Percent_Identity=24.4532803180915, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24652983, Length=503, Percent_Identity=24.4532803180915, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=25.9842519685039, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=23.8289205702648, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=23.8289205702648, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550774, Length=254, Percent_Identity=29.9212598425197, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21356731, Length=228, Percent_Identity=28.5087719298246, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=423, Percent_Identity=23.6406619385343, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=423, Percent_Identity=23.6406619385343, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52869; Mature: 52738

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRYESVENLLTLIKDKKIKPSDVVKDVYDAIEETDPTIKSFLALDKENAIKKAQELDE
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHH
LQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEEFVPIYESTVMEKLHNENAVLIG
HHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAAVAAGLVPFSLGSDTGGSIR
ECCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCCCCCCCCC
QPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEEECCCCCCCCCCC
ASVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLP
CCCCCCHHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIF
CCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVGPTAPTTAFNLGEEIDDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCHHCCCHHH
MYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHEECCCCCCHHHHHHHHHHHCCCCCHHH
VYEKL
HHHHC
>Mature Secondary Structure 
SIRYESVENLLTLIKDKKIKPSDVVKDVYDAIEETDPTIKSFLALDKENAIKKAQELDE
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHH
LQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEEFVPIYESTVMEKLHNENAVLIG
HHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAAVAAGLVPFSLGSDTGGSIR
ECCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCCCCCCCCC
QPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEEECCCCCCCCCCC
ASVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLP
CCCCCCHHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIF
CCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVGPTAPTTAFNLGEEIDDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCHHCCCHHH
MYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHEECCCCCCHHHHHHHHHHHCCCCCHHH
VYEKL
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA