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The map label for this gene is gatB

Identifier: 82751553

GI number: 82751553

Start: 1966094

End: 1967521

Strand: Reverse

Name: gatB

Synonym: SAB1832c

Alternate gene names: 82751553

Gene position: 1967521-1966094 (Counterclockwise)

Preceding gene: 82751554

Following gene: 82751552

Centisome position: 71.74

GC content: 34.8

Gene sequence:

>1428_bases
ATGCATTTTGAAACAGTTATAGGACTTGAAGTTCACGTAGAGTTAAAAACGGACTCAAAAATGTTTTCTCCATCACCAGC
GCATTTTGGAGCAGAACCTAACTCAAATACAAATGTTATCGACTTAGCATATCCAGGTGTCTTACCAGTTGTTAATAAGC
GTGCAGTAGACTGGGCAATGCGTGCTGCAATGGCACTAAATATGGAAATCGCAACAGAATCTAAGTTTGACCGTAAGAAC
TATTTCTATCCAGATAATCCAAAGGCATATCAAATTTCTCAATTTGATCAACCAATTGGTGAAAATGGATATATCGATAT
CGAAGTCGATGGTGAAACAAAACGAATCGGTATTACTCGTCTTCACATGGAAGAAGATGCTGGTAAGTCAACACATAAAG
GTGAGTATTCATTAGTTGACTTGAACCGTCAAGGTACACCGCTAATTGAAATCGTATCTGAACCAGATATTCGTTCACCT
ATAGAAGCATATGCATATTTAGAAAAATTACGTTCAATTATTCAATACACTGGTGTATCAGACGTTAAGATGGAAGAAGG
ATCTTTACGTTGTGATGCTAACATCTCTTTGCGTCCATATGGTCAAGAAAAATTTGGTACTAAAGCCGAATTGAAAAACT
TAAACTCATTTAACTATGTACGTAAAGGTTTAGAATATGAAGAAAAACGCCAAGAAGAAGAATTGTTAAATGGTGGAGAA
ATCGGACAAGAAACACGTCGATTTGATGAATCTACAGGTAAAACAATTTTAATGCGTGTTAAAGAAGGTTCTGATGATTA
CCGTTACTTCCCAGAGCCTGACATTGTACCTTTATATATTGATGATGCTTGGAAAAAGCGTGTTCGTCAGACAATTCCTG
AATTACCAGATGAGCGTAAAGCTAAGTATGTAAATGAATTAGGTTTACCTGCATACGATGCACACGTATTAACATTGACT
AAAGAAATGTCAGATTTCTTTGAATCAACAATTGAACACGGTGCAGATGTTAAATTGACATCTAACTGGTTAATGGGTGG
CGTAAACGAATATTTAAATAAAAATCAAGTAGAATTATTAGATACTAAATTAACACCAGAAAATTTAGCAGGTATGATTA
AGCTTATCGAAGACGGAACAATGAGCAGTAAAATTGCGAAGAAAGTCTTCCCAGAGTTAGCAGCTAAAGGTGGTAATGCT
AAACAGATTATGGAAGATAATGGCTTAGTTCAAATTTCTGATGAAGCAACACTTCTAAAATTTGTAAATGAAGCATTAGA
CAATAACGAACAATCAGTTGAAGATTACAAAAATGGTAAAGGCAAAGCTATGGGCTTCTTAGTTGGTCAAATTATGAAAG
CGTCTAAAGGTCAAGCTAATCCACAATTAGTAAATCAACTTTTAAAACAAGAATTAGATAAAAGATAA

Upstream 100 bases:

>100_bases
TAAACCATTCGATGAAAAAACGTTATATCGTGTCGCTTATCAATATGAAACACAATACAATTTACATGACGTTTATGAAA
AATTATAAGGAGTGGAAATC

Downstream 100 bases:

>100_bases
TTTTAATCATCAAACGATGAAGATTTAAAAAATAAACCCTTGATTGCTGACTTAGATGCAATCGAGGGTTTATTTATATC
TATAGAAGTCATATTACTTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKN
YFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
IEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLNGGE
IGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKKRVRQTIPELPDERKAKYVNELGLPAYDAHVLTLT
KEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNA
KQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELDKR

Sequences:

>Translated_475_residues
MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKN
YFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
IEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLNGGE
IGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKKRVRQTIPELPDERKAKYVNELGLPAYDAHVLTLT
KEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNA
KQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELDKR
>Mature_475_residues
MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKN
YFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
IEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLNGGE
IGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKKRVRQTIPELPDERKAKYVNELGLPAYDAHVLTLT
KEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNA
KQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELDKR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=36.8209255533199, Blast_Score=308, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI115534094, Length=475, Percent_Identity=30.7368421052632, Blast_Score=217, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=503, Percent_Identity=29.6222664015905, Blast_Score=208, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=32.9938900203666, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53641; Mature: 53641

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAM
CCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
RAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITR
HHHHHHCEEEECCCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCEEEEEECCCCEEEEEEE
LHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSPIEAYAYLEKLRSIIQYTGVS
EEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
DVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLNGGE
CEEECCCCEEECCCCEECCCCCHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCC
IGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKKRVRQTIPELPDERK
CCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCHHH
AKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVELL
HHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEECCHHHCCHHHHCCCCCEEEE
DTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEATLLK
ECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEECCCHHHHH
FVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELDKR
HHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAM
CCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
RAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITR
HHHHHHCEEEECCCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCEEEEEECCCCEEEEEEE
LHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSPIEAYAYLEKLRSIIQYTGVS
EEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
DVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLNGGE
CEEECCCCEEECCCCEECCCCCHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCC
IGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKKRVRQTIPELPDERK
CCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCHHH
AKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVELL
HHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEECCHHHCCHHHHCCCCCEEEE
DTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEATLLK
ECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEECCCHHHHH
FVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELDKR
HHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA