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The map label for this gene is brnQ [C]

Identifier: 82750999

GI number: 82750999

Start: 1376961

End: 1378304

Strand: Reverse

Name: brnQ [C]

Synonym: SAB1263c

Alternate gene names: 82750999

Gene position: 1378304-1376961 (Counterclockwise)

Preceding gene: 82751000

Following gene: 82750995

Centisome position: 50.26

GC content: 32.14

Gene sequence:

>1344_bases
ATGAATAAAAATACATGGGTCATTGGGTTTACGCTCTTCGCTATGTTTTTTGGCGCAGGCAATCTAATTTTTCCTCCTAA
TCTCGGATTGGATAGTGGTCAATTTTTCTGGCCCGCCATTTTAGCGTTTGTTCTAACTGGGATTGATTTACCATTATTAG
GTGTGATTGTAGGTGCACTTGATAAAGAAGGATATATTGGCGCATTAAATAAAATTTCACCTAAATTTTCAATATTGTTC
TTAATCATCATTTATTTGACTATAGGGCCACTTTTCGCAATACCTAGAACTGCATCTACATCTTTTGAAATGACAATTAC
ACCAATTATACATAGCAATAGTAGTATCGCTTTATTTATATTTACGATTATCTACTTCATAGTCGTTTTGTATATTTGTT
TAAATCCATCTAAGTTAATCGATCGTATTGGTTCATTATTAACACCATTATTATTGATTACTATTTTAGCGATGATTATT
AAGGGATACTTAGATTTTAGCGGTAATACTGCTGGAAAGGGCAATGAAGCACTATATCATTCTAATTTTTCAAGTTTTGC
TGAAGGCTTTACACAAGGCTATTTAACAATGGATGCCATTGCAGCAATTGCTTTTTCAATGATTGTTGTTAATGCAGTAA
AACTTACAGGTATTACTAAAACAAATCAAATATTCAAACAAACTTTGACTGCTGGTTTAATTGCAGCGGTTGCTTTAATT
TTCATATATATTTCATTAGGTTATATTGGTAATCATATGCCAGTAAGTGACATGACGTTAGATCAATTGAAATCCAAAGA
TCGAAACATTGGGACATATTTATTAACTACAATGGCTTCAACAGGATTTGGTTCATTCGGAAAATATTTATTGGGCATCA
TTGTGGCGTTGGCATGTCTAACTACAGCATGCGGACTTATTGGTGCAGTTTCTGAATATTTCCATAGAATCGTACCTAAA
GTATCATACAAAGCATTTGTATTAGTTTTCATTTTAATGAGTTTTATTATTGCTAACCAAGGTTTAAATGCTGTTATCTC
AATGTCAATTCCGGTATTAAGCATTGTATACCCAGTAGCAATAACTGTTGTGTTATTAATTTTAATTGCCAAATTCATAC
CGACAAAACGCATTTCACAACAAATTCCAGTTATTATCGTATTTATATTGTCGATTTTCAGTGTTATTAGTAAGTTAGGT
TGGCTGAAAATTAACTTTATAGAATCATTGCCTCTAAGAGCGTATTCTTTAGAGTGGTTCCCAGTAGCAATTATTGCAAC
GATATTAGGCTATCTAGTCGGCATATTTGTAAAACAAGATCCAATTAAATATCAACAGGAATAA

Upstream 100 bases:

>100_bases
TCTGCTATGTACAACATATTTCATATAGACTCTTTATTATTTTTTGTGCTAAAATATTTACTCGTAAATTATTTCGTGTT
TTAAAAAAAGGAGTAACTCT

Downstream 100 bases:

>100_bases
CGAATAATATAAAAGGAAGCATCTCCAATTTAATCTTAGGATGCTTCCTTTTTTGTTATATCTAATTCGTAATTTATGAT
TGTTTATTCGGTCCTTTGAT

Product: branched-chain amino acid carrier II protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIDLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNTAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIGAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE

Sequences:

>Translated_447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIDLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNTAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIGAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE
>Mature_447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIDLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNTAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIGAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine), which is coupled to a proton motive force (Potential). Contributes to NaCl tolerance [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=434, Percent_Identity=33.1797235023041, Blast_Score=228, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48845; Mature: 48845

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIDLPLLGVIVGAL
CCCCCEEHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
DKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFI
CCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHH
FTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGYLDFSGNTAGKGNEALYH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACL
HHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TTACGLIGAVSEYFHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWF
HHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCEEECCHH
PVAIIATILGYLVGIFVKQDPIKYQQE
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIDLPLLGVIVGAL
CCCCCEEHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
DKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFI
CCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHH
FTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGYLDFSGNTAGKGNEALYH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACL
HHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TTACGLIGAVSEYFHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWF
HHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCEEECCHH
PVAIIATILGYLVGIFVKQDPIKYQQE
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA