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The map label for this gene is trmD

Identifier: 82750844

GI number: 82750844

Start: 1192339

End: 1193076

Strand: Direct

Name: trmD

Synonym: SAB1104

Alternate gene names: 82750844

Gene position: 1192339-1193076 (Clockwise)

Preceding gene: 82750843

Following gene: 82750845

Centisome position: 43.48

GC content: 35.5

Gene sequence:

>738_bases
ATGAAAATTGATTATTTAACTTTATTTCCTGAAATGTTTGATGGTGTTTTAAATCATTCAATTATGAAACGTGCCCAAGA
AAACAATAAATTACAAATCAATACGGTTAATTTTAGAGATTATGCAATTAACAAGCACAACCAAGTAGATGATTATCCGT
ATGGTGGCGGACAAGGTATGGTGTTAAAGCCTGAACCTGTTTTTAATGCGATGGAAGACTTAGATGTCACAGAACAAACA
CGCGTTATTTTAATGTGTCCACAAGGCGAGCCATTTTCACATCAGAAAGCTGTTGAATTAAGCAAGGCTGACCACATCGT
TTTCATATGCGGACATTATGAAGGTTACGATGAACGTATCCGAACACATCTTGTCACAGATGAAATATCAATGGGTGACT
ATGTTTTAACAGGTGGAGAATTGCCAGCGATGACCATGACTGATGCTATTGTTAGGCTGATTCCAGGTGTTTTAGGTAAT
GAACAGTCACATCAAGACGATTCATTTTCAGATGGGTTATTAGAGTTTCCGCAATATACACGTCCGCGTGAATTTAAGGG
TCTAACAGTTCCAGATGTTTTATTGTCTGGAAATCATGCCAATATTGATGCATGGAGACATGAGCAAAAGTTGATCCGAA
CATATAATAAAAGACCTGACTTAATTGAAAAATATCCATTAACTAATGCAGATAAGCAAATATTAGAAAGATATAAAATA
GGATTGAAAAAAGGTTAG

Upstream 100 bases:

>100_bases
CTAAAGAATATTTGATTCCTTATATTGCTGATGTTGTAAAAGAAGTGGATGTTGAAAATAAAAAAATTATCATCACGCCA
ATGGAAGGATTGTTGGATTA

Downstream 100 bases:

>100_bases
TATTGTGCTAACATAATCTGAGTGTGGAAACACAAAAATCACTATGATCCGCTGCTATATATTTGTCGAGGCAAGAACAT
AGGTTGAAGAGGAGAATTTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQT
RVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
GLKKG

Sequences:

>Translated_245_residues
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQT
RVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
GLKKG
>Mature_245_residues
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQT
RVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
GLKKG

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=43.5684647302905, Blast_Score=217, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28056; Mature: 28056

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCE
VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
EECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCCHHHHH
RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
HHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
CCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHH
GLKKG
CCCCC
>Mature Secondary Structure
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCE
VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
EECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCCHHHHH
RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
HHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
CCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHH
GLKKG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA