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The map label for this gene is ndhF [H]

Identifier: 82750160

GI number: 82750160

Start: 437880

End: 439364

Strand: Direct

Name: ndhF [H]

Synonym: SAB0402

Alternate gene names: 82750160

Gene position: 437880-439364 (Clockwise)

Preceding gene: 82750159

Following gene: 82750161

Centisome position: 15.97

GC content: 37.1

Gene sequence:

>1485_bases
GTGTTAAGTTTTCAATTGCTATTTTCACTGTTTGTTATTGCGCTTATCATTGCATTGATAAGTGGCTTGTTGTTTTTAGC
ACCAGTTATGCCAATGAGATATATTAAATTACATTTATACATACTAGTCATGCCAGTATTATTTGCAGTCATTGGCTTTT
TCGGTATTCATGGTCAACATGTCTTAGGCCCATTTAAAATAGATCGTTTATCTTGGTTATTAGCTGGCTTTGTAATGGCG
CTTGGCTTTATTATTCAAAAGTTTTCAATGCGATATTTACTAGGTGATCATCATTATAGACATTACTTTCCATTGTTCAC
TGCGATTACGTCGTTTGCATCTTTAGCATGGATGTCTGAAGACTTAAGACTGATGGCACTCTGCTGGGGTATGACATTAT
TATGTTTAACATTGCTGATGAACGTTAATCGTTTTTGGAAAGTGCCACGTGAGTCTGCGAAATTATCAAGCATGACATTT
TTATGTGGTTGGCTTGCATTCGTTGGAGCAATTGTATCTATTTATATTGCAACTGGCGAGTGGCGGGTACCACAACATAT
AGCGCATCCGACATGGTCATTGTTGACGAATGTACTACTTGTATTAGCAGTCATGATACCGGCAGCACAATTTCCATTTC
ATCGATGGTTGATTGAATCTGTAACTGCACCAACGCCAGTATCGGCAATTATGCATGCAGGAATTGTGAATGCAGGTGGT
GTTATTCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCAGT
ATTGTTAGGATCAGGGATAAGTTTGGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCT
TTATGTTAGTTCAATGCGCATTGGGCGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACA
TTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGAT
TGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTGTTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAA
GTGCACTCATTCTAGCATGGTCATTACTTGTATCTTGGAATCAAATGGTAGCCTTTAGTAAAGGGCGTATGGCACGTCTG
GTTGGCATGATTTTGATTTCAATTGTGACATTTATCTACATCATCACACATAATTATTTTTACGATGTATTACAAAATAT
AACAACACATGCGACAACACCGCCTACAATTAGTGTCATCATTAGTGTTGTTATATTAATATTTGGTAGTTTATTAAGTA
TCTGGGTAGCGCGTCATCGATACTCTAAGGCTTTTGCGGTATTGTACGTGTGGTTAGTTAATCTAGGTGAAGCACGCTCG
AAAGCGATAGAAAGTCATCCGAATTATTTGAAGAAGTATTTATAG

Upstream 100 bases:

>100_bases
ATAGTAAATTTTCTGCCATTATGTACAGAATCTACTATTGTAGGTTTTTATTTTTATACATAAGCATTATGCGAGAGATT
AGAAATAAGGAGGTTATGCA

Downstream 100 bases:

>100_bases
GAGGTGAAAGGTATGACAACACAGTTAAATATCAATTTAGTCATTGAAAATGCGAAACGTGTTATTACACCATTATCACC
GATTTCGATTTTTGCAGCAC

Product: NADH dehydrogenase subunit 5

Products: NA

Alternate protein names: NADH dehydrogenase subunit 5 [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMA
LGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
LCGWLAFVGAIVSIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGG
VILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKAT
LFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAFSKGRMARL
VGMILISIVTFIYIITHNYFYDVLQNITTHATTPPTISVIISVVILIFGSLLSIWVARHRYSKAFAVLYVWLVNLGEARS
KAIESHPNYLKKYL

Sequences:

>Translated_494_residues
MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMA
LGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
LCGWLAFVGAIVSIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGG
VILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKAT
LFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAFSKGRMARL
VGMILISIVTFIYIITHNYFYDVLQNITTHATTPPTISVIISVVILIFGSLLSIWVARHRYSKAFAVLYVWLVNLGEARS
KAIESHPNYLKKYL
>Mature_494_residues
MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMA
LGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
LCGWLAFVGAIVSIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGG
VILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKAT
LFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAFSKGRMARL
VGMILISIVTFIYIITHNYFYDVLQNITTHATTPPTISVIISVVILIFGSLLSIWVARHRYSKAFAVLYVWLVNLGEARS
KAIESHPNYLKKYL

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=278, Percent_Identity=28.0575539568345, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788614, Length=405, Percent_Identity=28.641975308642, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788831, Length=154, Percent_Identity=32.4675324675325, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788829, Length=281, Percent_Identity=26.6903914590747, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55335; Mature: 55335

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTFLCGWLAFVGAIVSIYIATGE
HHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGG
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
VILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
LGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FWMSSDRSAYEVLSALILAWSLLVSWNQMVAFSKGRMARLVGMILISIVTFIYIITHNYF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YDVLQNITTHATTPPTISVIISVVILIFGSLLSIWVARHRYSKAFAVLYVWLVNLGEARS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAIESHPNYLKKYL
HHHHCCHHHHHHCC
>Mature Secondary Structure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HHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]