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The map label for this gene is ldh1 [H]

Identifier: 82749948

GI number: 82749948

Start: 225690

End: 226643

Strand: Direct

Name: ldh1 [H]

Synonym: SAB0180

Alternate gene names: 82749948

Gene position: 225690-226643 (Clockwise)

Preceding gene: 82749943

Following gene: 82749950

Centisome position: 8.23

GC content: 37.21

Gene sequence:

>954_bases
ATGAACAAATTTAAAGGGAACAAAGTTGTATTAATTGGTAATGGTGCAGTAGGTTCAAGCTACGCATTTTCATTAGTGAA
CCAAAGCATTGTTGATGAATTAGTCATCATTGATTTAGACACTGAAAAAGTTCGAGGAGATGTTATGGATTTAAAACATG
CCACACCATATTCTCCAACAACAGTTCGTGTGAAAGCTGGCGAATACAGTGATTGCCATGATGCGGATCTAGTTGTCATC
TGTGCTGGTGCTGCACAAAAACCTGGAGAAACACGTTTAGATTTAGTATCTAAAAACTTGAAAATATTCAAATCAATTGT
TGGTGAAGTAATGGCATCAAAATTTGATGGTATTTTCTTGGTAGCTACAAATCCTGTTGATATTTTAGTGTATGCAACAT
GGAAATTCTCTGGTTTACCTAAAGAACGTGTTATAGGTTCTGGTACAATTTTAGACTCTGCACGCTTTAGATTATTGTTA
AGCGAAGCGTTCGATGTTGCGCCACGTAGCGTCGATGCTCAAATTATTGGTGAACATGGTGACACTGAATTACCAGTATG
GTCACACGCTAATATTGCGGGTCAACCTTTGAAGACATTACTTGAACAACGTCCTGAGGGCAAAGCGCAAATTGAACAAA
TTTTTGTTCAAACACGTGATGCAGCATATGATATTATTCAAGCTAAAGGTGCCACTTATTATGGTGTTGCAATGGGATTA
GCTAGAATTACTGAAGCGATTTTCAGAAATGAAGATGCCGTATTGACTGTATCAGCATTATTAGAAGGCGAATATGATGA
AGAAGATGTTTATATTGGTGTTCCAGCAGTCATCAATAGAAACGGTATTCGCAACGTCGTAGAAATCCCATTAAACGACG
AGGAACAAAGCAAGTTCGCACATTCAGCTAAAACATTAAAAGATATTATGGCTGAAGCAGAAGAACTTAAATAA

Upstream 100 bases:

>100_bases
GAGATGTTTATTTGCTTTTGTATCGTCAATAAGCAGCATTAAACTAACATATTTGAAACTACATGTATGTTAGTGAATTA
ATCATAAGGGAGTTTTTGTA

Downstream 100 bases:

>100_bases
CTTTTTATAAAATCTATACCATTCCAAAAATTGTAAAACCTTACCCCAAAAAATTGTATAAAGGGCTATTTGATACAACT
ATATGTGTCGAGTAGCCCTA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 1 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVI
CAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDILVYATWKFSGLPKERVIGSGTILDSARFRLLL
SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK

Sequences:

>Translated_317_residues
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVI
CAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDILVYATWKFSGLPKERVIGSGTILDSARFRLLL
SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK
>Mature_317_residues
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVI
CAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFLVATNPVDILVYATWKFSGLPKERVIGSGTILDSARFRLLL
SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK

Specific function: Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=40.3846153846154, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=40.3846153846154, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI5031857, Length=311, Percent_Identity=39.871382636656, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI260099723, Length=311, Percent_Identity=39.871382636656, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI15082234, Length=309, Percent_Identity=39.1585760517799, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=40.9836065573771, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=40.9836065573771, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI291575128, Length=316, Percent_Identity=37.3417721518987, Blast_Score=237, Evalue=9e-63,
Organism=Homo sapiens, GI4557032, Length=316, Percent_Identity=37.3417721518987, Blast_Score=237, Evalue=9e-63,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=45.8762886597938, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI260099725, Length=194, Percent_Identity=45.8762886597938, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI207028494, Length=214, Percent_Identity=39.7196261682243, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI103472011, Length=308, Percent_Identity=27.5974025974026, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=29.6703296703297, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI21735621, Length=289, Percent_Identity=24.9134948096886, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789632, Length=327, Percent_Identity=26.9113149847095, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=39.3835616438356, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17554310, Length=301, Percent_Identity=26.578073089701, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322765, Length=196, Percent_Identity=30.6122448979592, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17136226, Length=309, Percent_Identity=39.1585760517799, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45550422, Length=326, Percent_Identity=29.7546012269939, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24647881, Length=316, Percent_Identity=28.7974683544304, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24663599, Length=326, Percent_Identity=23.6196319018405, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34598; Mature: 34598

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPT
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHCHHHHCCCCCCCCCE
TVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFL
EEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VATNPVDILVYATWKFSGLPKERVIGSGTILDSARFRLLLSEAFDVAPRSVDAQIIGEHG
EECCCEEEEEEEEEEECCCCHHHEECCCCEEHHHHHHHHHHHHHHCCCCCCCEEEECCCC
DTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
CCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFA
HHHHHHHHCCCCCEEEEEHHHCCCCCCCCEEECCCCEECCCCCCCEEECCCCCCHHHHHH
HSAKTLKDIMAEAEELK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKVRGDVMDLKHATPYSPT
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCHHHHHCHHHHCCCCCCCCCE
TVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKIFKSIVGEVMASKFDGIFL
EEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VATNPVDILVYATWKFSGLPKERVIGSGTILDSARFRLLLSEAFDVAPRSVDAQIIGEHG
EECCCEEEEEEEEEEECCCCHHHEECCCCEEHHHHHHHHHHHHHHCCCCCCCEEEECCCC
DTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQTRDAAYDIIQAKGATYYGVAMGL
CCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
ARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPAVINRNGIRNVVEIPLNDEEQSKFA
HHHHHHHHCCCCCEEEEEHHHCCCCCCCCEEECCCCEECCCCCCCEEECCCCCCHHHHHH
HSAKTLKDIMAEAEELK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA