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The map label for this gene is rihA [H]

Identifier: 82749950

GI number: 82749950

Start: 228854

End: 229789

Strand: Direct

Name: rihA [H]

Synonym: SAB0182

Alternate gene names: 82749950

Gene position: 228854-229789 (Clockwise)

Preceding gene: 82749948

Following gene: 82749951

Centisome position: 8.34

GC content: 39.42

Gene sequence:

>936_bases
ATGAATAGAAAGATTATTATGGATTGTGATCCAGGACACGATGATGCAATAGCATTAATTTTAGCGGGGGCAATTGACAG
TCCACTAGAGATATTAGCTATAACCACAGTCGCAGGTAATCAATCAGTTGACAAGAATACGACAAACGCCTTGAACGTAT
TGGATATTATAGGACGTCAAGATATAGCAGTAGCGAAAGGTGCGGATAGGCCGTTAATTAAACCAGCTGCCTTTGCTTCT
GAAATACATGGGGAATCTGGATTAGATGGTCCGAAACTACCGTCGACACCATCACGTCAAGCAGTTGCAATGCCAGCATC
AGATGTGATTATTAACAAAGTGATGACGAGTGATACACCTGTAACAATTGTAGCGACAGGTCCTCTTACGAATATAGCAA
CGGCATTGATTCGTGAGCCAAGAATCGCTGAGCATATTGAATCTATTACTTTGATGGGTGGTGGTACATTTGGAAATTGG
ACGCCTACAGCAGAATTCAATATTTGGGTAGATGCTGAAGCAGCGAAGCGTGTTTTTGAAAGTGGGATTACTATAAATGT
GTTTGGTTTAGATGTAACACATCAAGTTTTAGCCGACGATCACGTGATTGAACGCTTTGAAAGTATCAATAATCCTGTTG
CACAGTTCGTCGTAGAATTATTGCAATTCTTTAAGAAGACATACAAGACTCACTTTAATATGGACGGTGGTCCAATACAT
GATGCTTGTACGATTTTGTATTTGTTACAGCCAGAATTGTTTACAATGGTACCTGTTAATATCGACATTGAACATCAAAG
TCCACTAACTTATGGCACTATGGCTGTCGATTTAAATCATGTTACAGGTAAGCCTGCCAATGCTTATTTTGCTACAGCAG
TTGATGTTGAAGAAGTGTGGAACTTGATAGATCATAAGTTACGTACATACGAATAA

Upstream 100 bases:

>100_bases
TATGAGAAAGTTAAAACTATTTTTCAAAATAAACATGATTCACCACATAAATAAATATACATAAGCTATCGATAACAACT
TAAGAAAGGTGGATATATAA

Downstream 100 bases:

>100_bases
TAATACTTAATTAAATGGATACAGTTAGCCCTAAGGTGCCTGATTTAAGCGTCCTTAGGGTTTTTGTATTGGATTACAAC
GGTCATGACAATTTGATAAT

Product: inosine-uridine preferring nucleoside hydrolase family protein

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNRKIIMDCDPGHDDAIALILAGAIDSPLEILAITTVAGNQSVDKNTTNALNVLDIIGRQDIAVAKGADRPLIKPAAFAS
EIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTPVTIVATGPLTNIATALIREPRIAEHIESITLMGGGTFGNW
TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE

Sequences:

>Translated_311_residues
MNRKIIMDCDPGHDDAIALILAGAIDSPLEILAITTVAGNQSVDKNTTNALNVLDIIGRQDIAVAKGADRPLIKPAAFAS
EIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTPVTIVATGPLTNIATALIREPRIAEHIESITLMGGGTFGNW
TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE
>Mature_311_residues
MNRKIIMDCDPGHDDAIALILAGAIDSPLEILAITTVAGNQSVDKNTTNALNVLDIIGRQDIAVAKGADRPLIKPAAFAS
EIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTPVTIVATGPLTNIATALIREPRIAEHIESITLMGGGTFGNW
TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=313, Percent_Identity=45.3674121405751, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1788486, Length=311, Percent_Identity=40.8360128617363, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1786213, Length=298, Percent_Identity=38.9261744966443, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=258, Percent_Identity=36.046511627907, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24583915, Length=195, Percent_Identity=31.7948717948718, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641839, Length=201, Percent_Identity=32.3383084577114, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33791; Mature: 33791

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00018 EF_HAND_1 ; PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKIIMDCDPGHDDAIALILAGAIDSPLEILAITTVAGNQSVDKNTTNALNVLDIIGRQ
CCCCEEEECCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCC
DIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTP
CEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCC
VTIVATGPLTNIATALIREPRIAEHIESITLMGGGTFGNWTPTAEFNIWVDAEAAKRVFE
EEEEECCCHHHHHHHHHCCCHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECHHHHHHHH
SGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVW
HHHHEEEEECCCEEEEEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEEEECCHHHHH
NLIDHKLRTYE
HHHHHHHHCCC
>Mature Secondary Structure
MNRKIIMDCDPGHDDAIALILAGAIDSPLEILAITTVAGNQSVDKNTTNALNVLDIIGRQ
CCCCEEEECCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCC
DIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAVAMPASDVIINKVMTSDTP
CEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCC
VTIVATGPLTNIATALIREPRIAEHIESITLMGGGTFGNWTPTAEFNIWVDAEAAKRVFE
EEEEECCCHHHHHHHHHCCCHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECHHHHHHHH
SGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVVELLQFFKKTYKTHFNMDGGPIH
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAVDLNHVTGKPANAYFATAVDVEEVW
HHHHEEEEECCCEEEEEEEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEEEECCHHHHH
NLIDHKLRTYE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA