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The map label for this gene is rsmD [H]

Identifier: 82703848

GI number: 82703848

Start: 3140125

End: 3140664

Strand: Reverse

Name: rsmD [H]

Synonym: Nmul_A2735

Alternate gene names: 82703848

Gene position: 3140664-3140125 (Counterclockwise)

Preceding gene: 82703849

Following gene: 82703847

Centisome position: 98.63

GC content: 54.81

Gene sequence:

>540_bases
ATGAGAAATAGAGTTCGAATCATAGGAGGTGAGTGGCGCAGCCGCATCCTGACTTTTCCCAACGATATGGACCTGCGTCC
CACCCCTGACCGGGTGCGCGAGACGGTCTTCAATTGGCTAGGGCAGGATATGAGCCACAAACGCTGCCTCGATCTGTTTG
CAGGCAGCGGCGCAATGGGTTTCGAAGCAGCTTCGCGTGGAGCGGCGGAAGTCGTGATGGTGGAATCCAGCCCTGCGGTA
TTGAAAGCGCTTCGCGCCACCGCCGAGAAACTGGGGGCAACGCGCATTGAACTGGCGGGAATCGATGCCATGAATTTTAT
TGATTCCGATCGGCGCCGCTTCGATGTGATATTTCTCGATCCACCTTATCGTCTCGATTTGTTGCCCAGGCTACTGCCCA
AATTGCACGCACATCTTGCCGATGGAGGAATGGTCTATCTGGAAGACGATTGCCCCGCGGAGTTTGAAGGATGGGCAGTG
TGGCGCAGGGGACATGCAGGAAAGGTGTGCTATCACTTGTTAAGCTCCACGATAAATTAA

Upstream 100 bases:

>100_bases
CCGTGGTGGTGGGTCTTCCGGACACGAGTGGAAAGTAAAAAACTTTTTGCAAGCCGCTTCCTCCTGACAGACGTTTTTCT
CTTTTTTCATCGCATGCACG

Downstream 100 bases:

>100_bases
ATGGATAAAGCGATATACCCCGGTACTTTCGATCCCATTACGCGGGGTCATGAAGATCTGGTGCGACGTGCCTCCGGTTT
GTTCAGGGAGGTGGTGGTAG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MRNRVRIIGGEWRSRILTFPNDMDLRPTPDRVRETVFNWLGQDMSHKRCLDLFAGSGAMGFEAASRGAAEVVMVESSPAV
LKALRATAEKLGATRIELAGIDAMNFIDSDRRRFDVIFLDPPYRLDLLPRLLPKLHAHLADGGMVYLEDDCPAEFEGWAV
WRRGHAGKVCYHLLSSTIN

Sequences:

>Translated_179_residues
MRNRVRIIGGEWRSRILTFPNDMDLRPTPDRVRETVFNWLGQDMSHKRCLDLFAGSGAMGFEAASRGAAEVVMVESSPAV
LKALRATAEKLGATRIELAGIDAMNFIDSDRRRFDVIFLDPPYRLDLLPRLLPKLHAHLADGGMVYLEDDCPAEFEGWAV
WRRGHAGKVCYHLLSSTIN
>Mature_179_residues
MRNRVRIIGGEWRSRILTFPNDMDLRPTPDRVRETVFNWLGQDMSHKRCLDLFAGSGAMGFEAASRGAAEVVMVESSPAV
LKALRATAEKLGATRIELAGIDAMNFIDSDRRRFDVIFLDPPYRLDLLPRLLPKLHAHLADGGMVYLEDDCPAEFEGWAV
WRRGHAGKVCYHLLSSTIN

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=176, Percent_Identity=39.7727272727273, Blast_Score=126, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 20154; Mature: 20154

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRVRIIGGEWRSRILTFPNDMDLRPTPDRVRETVFNWLGQDMSHKRCLDLFAGSGAMG
CCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
FEAASRGAAEVVMVESSPAVLKALRATAEKLGATRIELAGIDAMNFIDSDRRRFDVIFLD
CHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCEEEEEEEC
PPYRLDLLPRLLPKLHAHLADGGMVYLEDDCPAEFEGWAVWRRGHAGKVCYHLLSSTIN
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRNRVRIIGGEWRSRILTFPNDMDLRPTPDRVRETVFNWLGQDMSHKRCLDLFAGSGAMG
CCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
FEAASRGAAEVVMVESSPAVLKALRATAEKLGATRIELAGIDAMNFIDSDRRRFDVIFLD
CHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCEEEEEEEC
PPYRLDLLPRLLPKLHAHLADGGMVYLEDDCPAEFEGWAVWRRGHAGKVCYHLLSSTIN
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]