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The map label for this gene is hemK [H]

Identifier: 82703753

GI number: 82703753

Start: 3025450

End: 3026310

Strand: Reverse

Name: hemK [H]

Synonym: Nmul_A2640

Alternate gene names: 82703753

Gene position: 3026310-3025450 (Counterclockwise)

Preceding gene: 82703754

Following gene: 82703752

Centisome position: 95.04

GC content: 59.47

Gene sequence:

>861_bases
GTGTCGTCCCTCACCATTTTCGAAGCGCTGGGACAAGCCCGCCGGAACATTGCCGATATCGACGCGCGGATGCTGTTGCA
GTACGTGCTCGACGTAAATCATGCTTACCTTCTTGCTCATCCCGAGCAGAAACTGACCGCTGAGCAGGCGCAGGCGTTTT
ACCTGCTGACAGGCCGGCGCACCCTTGGTGAACCGGTTGCTTATCTGACGGGGAGGCGCGAGTTCTACAGTCTCGAGTTC
AACGTCACGCCTGCGGTACTCGTTCCCCGACCCGAAACCGAATTATTGATCGATCTCGCGCTGGCGCGGATTCCTCCGCA
GCGTCCCTGCAGGATACTTGATCTGGGGACGGGCAGCGGCGCTATCGCGCTGACCCTGGCCACACATCGACCGCTGGCAA
AGGTTACCGCAGTCGATGTTTCAGCCGCCGCGGTCGCCGTTGCCAACGCCAACGCCGCCCGCTTCAGCCTGAATAATGTG
CGTGTTATCGAGGCAGACTGGTTTGAGGGAGTGGCTGGCGAAAGCTTCGATCTGATCGTCTCCAACCCGCCTTATATAGC
GGGGGACGATCCTCATCTGGCCCAGGGCGATCTGCGCTTCGAGCCCCAGATCGCGCTCGTGGCCCCAAGCGAAGGCCTTG
GCTGTATCCGGTTGATCATTGATTCCGCTCCCGCTCATCTGGCGCCCGAGGGGTCACTGCTGCTGGAACACGGTTACGAT
CAGGCGGAAGCTTGCAGATGCCTCCTGAGGGAAGCGGGTTTCAGGGAGGTGTTCTCCCAGCCCGATCTCGCTGGAATAAT
GCGGGTAAGTGGTGGATGGTTTAGGAGTTTCCCGGGAGAAGCTGCTAAAATGCGATATTAA

Upstream 100 bases:

>100_bases
AGCAGATCATGGATGGGGATTTGAACGAGCTTTGCTCGGCCCTGCTGGCCGAGCATCAGGCCGAGCAGTTGGCGGCAATG
GCGGAGTAGATTACCGGCCG

Downstream 100 bases:

>100_bases
TACGATATATCGAAGAATTGCCTCCTCCTGGAGGGCTTTCCCCCGATAATCCGAACCAACGTTTTTTGAGGAGTCAGATA
TGGATACGCAGGAAATCATC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSSLTIFEALGQARRNIADIDARMLLQYVLDVNHAYLLAHPEQKLTAEQAQAFYLLTGRRTLGEPVAYLTGRREFYSLEF
NVTPAVLVPRPETELLIDLALARIPPQRPCRILDLGTGSGAIALTLATHRPLAKVTAVDVSAAAVAVANANAARFSLNNV
RVIEADWFEGVAGESFDLIVSNPPYIAGDDPHLAQGDLRFEPQIALVAPSEGLGCIRLIIDSAPAHLAPEGSLLLEHGYD
QAEACRCLLREAGFREVFSQPDLAGIMRVSGGWFRSFPGEAAKMRY

Sequences:

>Translated_286_residues
MSSLTIFEALGQARRNIADIDARMLLQYVLDVNHAYLLAHPEQKLTAEQAQAFYLLTGRRTLGEPVAYLTGRREFYSLEF
NVTPAVLVPRPETELLIDLALARIPPQRPCRILDLGTGSGAIALTLATHRPLAKVTAVDVSAAAVAVANANAARFSLNNV
RVIEADWFEGVAGESFDLIVSNPPYIAGDDPHLAQGDLRFEPQIALVAPSEGLGCIRLIIDSAPAHLAPEGSLLLEHGYD
QAEACRCLLREAGFREVFSQPDLAGIMRVSGGWFRSFPGEAAKMRY
>Mature_285_residues
SSLTIFEALGQARRNIADIDARMLLQYVLDVNHAYLLAHPEQKLTAEQAQAFYLLTGRRTLGEPVAYLTGRREFYSLEFN
VTPAVLVPRPETELLIDLALARIPPQRPCRILDLGTGSGAIALTLATHRPLAKVTAVDVSAAAVAVANANAARFSLNNVR
VIEADWFEGVAGESFDLIVSNPPYIAGDDPHLAQGDLRFEPQIALVAPSEGLGCIRLIIDSAPAHLAPEGSLLLEHGYDQ
AEACRCLLREAGFREVFSQPDLAGIMRVSGGWFRSFPGEAAKMRY

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=210, Percent_Identity=36.1904761904762, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1787463, Length=254, Percent_Identity=48.8188976377953, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI87082085, Length=175, Percent_Identity=40.5714285714286, Blast_Score=114, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24582226, Length=194, Percent_Identity=31.4432989690722, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31105; Mature: 30974

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLTIFEALGQARRNIADIDARMLLQYVLDVNHAYLLAHPEQKLTAEQAQAFYLLTGRR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCEEEEEECCC
TLGEPVAYLTGRREFYSLEFNVTPAVLVPRPETELLIDLALARIPPQRPCRILDLGTGSG
CCCCCHHHHCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
AIALTLATHRPLAKVTAVDVSAAAVAVANANAARFSLNNVRVIEADWFEGVAGESFDLIV
EEEEEEECCCCCCEEEEEECCHHEEEEECCCCEEEEECCEEEEEECCCCCCCCCCEEEEE
SNPPYIAGDDPHLAQGDLRFEPQIALVAPSEGLGCIRLIIDSAPAHLAPEGSLLLEHGYD
CCCCEECCCCCCCCCCCEEECCEEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCC
QAEACRCLLREAGFREVFSQPDLAGIMRVSGGWFRSFPGEAAKMRY
HHHHHHHHHHHCCHHHHHCCCCHHEEEEECCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SSLTIFEALGQARRNIADIDARMLLQYVLDVNHAYLLAHPEQKLTAEQAQAFYLLTGRR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCEEEEEECCC
TLGEPVAYLTGRREFYSLEFNVTPAVLVPRPETELLIDLALARIPPQRPCRILDLGTGSG
CCCCCHHHHCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
AIALTLATHRPLAKVTAVDVSAAAVAVANANAARFSLNNVRVIEADWFEGVAGESFDLIV
EEEEEEECCCCCCEEEEEECCHHEEEEECCCCEEEEECCEEEEEECCCCCCCCCCEEEEE
SNPPYIAGDDPHLAQGDLRFEPQIALVAPSEGLGCIRLIIDSAPAHLAPEGSLLLEHGYD
CCCCEECCCCCCCCCCCEEECCEEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCC
QAEACRCLLREAGFREVFSQPDLAGIMRVSGGWFRSFPGEAAKMRY
HHHHHHHHHHHCCHHHHHCCCCHHEEEEECCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]