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The map label for this gene is thiE

Identifier: 82703741

GI number: 82703741

Start: 3011121

End: 3011825

Strand: Direct

Name: thiE

Synonym: Nmul_A2628

Alternate gene names: 82703741

Gene position: 3011121-3011825 (Clockwise)

Preceding gene: 82703740

Following gene: 82703742

Centisome position: 94.56

GC content: 54.75

Gene sequence:

>705_bases
ATGAGAACGGTGTCGAGGAACGAAATCAAGCGATGTAGCGCTGCTCCGCGTCGAGAAATAACCGGTCTATATGCGATCAC
ACCAGATACAGGCGATACATCGCATCTGGTCGCGATGACATGGAGGGCGCTTGCCGGAGGTGCGCGACTGGTTCAATACC
GCAGCAAAACGCTGAATGAGGAACTGCACCGGGAACAGGCGCGCTCCCTCGCCCATCTCTGCCGCCGGTTTGACGTTCCC
CTCCTCATCAACGATCATATCGATCTTGCTCTGGAGGTCGGGGCGGATGGAGTGCATCTGGGACGGGAAGATGCACCCAT
TTCTCAAGCCCGGCTTAAACTGGGTCATGAAAAGATCATCGGAATTTCCTGCTACAACGAACTCGAATCTGCCATCGAAG
CCGAGTGCAATGGGGCGGATTACGTCGCTTTCGGGGCTTTTTTCAATTCCGTAACAAAGACCGATACCGTGCCCGCTTCC
ATCGATCTGCTACGGCAGGGAAATCTTGAGATCAGGATTCCCATTGTCGCCATAGGCGGCATCAATAGCGATAATGCCCT
GGAGTTGATCAGCGCCGGCGCGGATGCTGTAGCAGTCAGCAACGCCCTGTTTGGCGCACGAGATATCCGTTCCGAGGCCG
CCAAGTTCTCCGGATTATTCAAGCGTCAGTCGTTTCACCCCTCATTGTCAAGGACCAGTCAATAA

Upstream 100 bases:

>100_bases
GGTGTATATGAAGCGCAGGAATACACCTGGCAATCGCTGAAAGCCGGGTTCAGGCCTGGAATGGGGCAACATCTGCCAGA
CAGGCTATTCTGGGCGCGTG

Downstream 100 bases:

>100_bases
TGTCACGCAACCAGCAATTGTTCGAACAGTCGCAAAAATTCATTCCCGGTGGTGTCAACTCGCCGGTTCGCGCCTTCAAA
TCGGTAGGCGGCACGCCGGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRTVSRNEIKRCSAAPRREITGLYAITPDTGDTSHLVAMTWRALAGGARLVQYRSKTLNEELHREQARSLAHLCRRFDVP
LLINDHIDLALEVGADGVHLGREDAPISQARLKLGHEKIIGISCYNELESAIEAECNGADYVAFGAFFNSVTKTDTVPAS
IDLLRQGNLEIRIPIVAIGGINSDNALELISAGADAVAVSNALFGARDIRSEAAKFSGLFKRQSFHPSLSRTSQ

Sequences:

>Translated_234_residues
MRTVSRNEIKRCSAAPRREITGLYAITPDTGDTSHLVAMTWRALAGGARLVQYRSKTLNEELHREQARSLAHLCRRFDVP
LLINDHIDLALEVGADGVHLGREDAPISQARLKLGHEKIIGISCYNELESAIEAECNGADYVAFGAFFNSVTKTDTVPAS
IDLLRQGNLEIRIPIVAIGGINSDNALELISAGADAVAVSNALFGARDIRSEAAKFSGLFKRQSFHPSLSRTSQ
>Mature_234_residues
MRTVSRNEIKRCSAAPRREITGLYAITPDTGDTSHLVAMTWRALAGGARLVQYRSKTLNEELHREQARSLAHLCRRFDVP
LLINDHIDLALEVGADGVHLGREDAPISQARLKLGHEKIIGISCYNELESAIEAECNGADYVAFGAFFNSVTKTDTVPAS
IDLLRQGNLEIRIPIVAIGGINSDNALELISAGADAVAVSNALFGARDIRSEAAKFSGLFKRQSFHPSLSRTSQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=201, Percent_Identity=30.3482587064677, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325042, Length=177, Percent_Identity=32.2033898305085, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_NITMU (Q2Y5Q6)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413307.1
- ProteinModelPortal:   Q2Y5Q6
- SMR:   Q2Y5Q6
- STRING:   Q2Y5Q6
- GeneID:   3786331
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2628
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   CYNELES
- PhylomeDB:   Q2Y5Q6
- ProtClustDB:   PRK00043
- BioCyc:   NMUL323848:NMUL_A2628-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 25453; Mature: 25453

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTVSRNEIKRCSAAPRREITGLYAITPDTGDTSHLVAMTWRALAGGARLVQYRSKTLNE
CCCCCHHHHHHHCCCCCHHCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
ELHREQARSLAHLCRRFDVPLLINDHIDLALEVGADGVHLGREDAPISQARLKLGHEKII
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEECCCCCCCHHHHHHHCCCHHEE
GISCYNELESAIEAECNGADYVAFGAFFNSVTKTDTVPASIDLLRQGNLEIRIPIVAIGG
EEHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEEEEEC
INSDNALELISAGADAVAVSNALFGARDIRSEAAKFSGLFKRQSFHPSLSRTSQ
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRTVSRNEIKRCSAAPRREITGLYAITPDTGDTSHLVAMTWRALAGGARLVQYRSKTLNE
CCCCCHHHHHHHCCCCCHHCCEEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHH
ELHREQARSLAHLCRRFDVPLLINDHIDLALEVGADGVHLGREDAPISQARLKLGHEKII
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEECCCCCCCHHHHHHHCCCHHEE
GISCYNELESAIEAECNGADYVAFGAFFNSVTKTDTVPASIDLLRQGNLEIRIPIVAIGG
EEHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEEEEEC
INSDNALELISAGADAVAVSNALFGARDIRSEAAKFSGLFKRQSFHPSLSRTSQ
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA