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The map label for this gene is hemL

Identifier: 82703742

GI number: 82703742

Start: 3011825

End: 3013102

Strand: Direct

Name: hemL

Synonym: Nmul_A2629

Alternate gene names: 82703742

Gene position: 3011825-3013102 (Clockwise)

Preceding gene: 82703741

Following gene: 82703744

Centisome position: 94.59

GC content: 56.81

Gene sequence:

>1278_bases
ATGTCACGCAACCAGCAATTGTTCGAACAGTCGCAAAAATTCATTCCCGGTGGTGTCAACTCGCCGGTTCGCGCCTTCAA
ATCGGTAGGCGGCACGCCGGTTTTCTTCAGAAAAGGTGAGGGTGCCTATGCCTGGGATGCGGATGACAAGTCCTACATAG
ATTATGTCGGTTCCTGGGGCCCTCTGATTCTCGGGCATGCCCATCCTGAAGTGGTTGATGCGGTCTATGCGGCAGCAAAA
AACGGCTTGACTTTTGGAGCGCCCACCGAGGCGGAACTGGAAATAGCGGAACTGCTGTGCCGACTGGTGCCGTCAATCGA
GCAAGTCAGGCTGGTAAGCTCCGGAACCGAAGCGACCATGAGCGCCATCCGGCTGGCGCGCGGTTATACCGCTCGGAATC
GCATCATCAAGTTTGAAGGCTGTTATCACGGCCACGACGACGCCTTGCTGGTGAAGGCAGGCTCTGGCGCTCTGACTTTC
GGCCATCCGAGTTCTGCCGGCGTCCCTGTTGAAACGGCTTCGAGCACGGTGGTGCTCGATTACAATGATCTCGCCGGTGT
TGAACAGGCTTTCAACCAGTTCGGTGCCGAAATCGCGGCAGTCATTGTCGAGCCTGTTGCGGGCAACATGAATCTTATCG
CGCCTCAGCCCGGCTTCCTGGCGGGTTTGCGCGAATTGTGCACGCGCCACGGCAGCGTGTTGATCTTCGATGAAGTGATG
ACAGGCTTTCGCGTCGGACTGGAGTGTGCCCAGGGGCTTTACGGAATAAAACCCGATCTCACCACACTGGGCAAAGTGAT
TGGCGGCGGTATGCCAATGGCTGCGTTTGGAGGCCGGCGAGAAATCATGCAATGCCTTGCACCAGTAGGAGCCGTCTATC
AAGCGGGTACGCTTTCGGGAAATCCGGTTGCGGTCGCTGCCGGCCTCGCTACATTGAAGCTGGTGCAGGTACCGGGGTTC
TACGACAAACTCGCGGCGCGTACCCGCAAACTTACGGAAGGTCTTGCCGCGGCCGCAGCAAAACAGGGTGTGGTTTTTTG
TGCGGAGGCTGTAGGGGGCATGTTCGGACTCTATTTCCGGGAGAGCGCGCCGAAAAGTTACGCGGAGGTGATGAGCTGTG
ACCGCGAGGCTTTCAATGGTTTTTTCCACGCCATGCTCGAGGAGGGTATTTATTTCGCCCCATCAGCATTTGAAGCCGGA
TTTGTTTCCGCGGCACACGGGGATGCTGAAATCAGCAAAACACTAGCCACCGCTGAAAAAATCTTTGCGCGGGAATAA

Upstream 100 bases:

>100_bases
CCCTGTTTGGCGCACGAGATATCCGTTCCGAGGCCGCCAAGTTCTCCGGATTATTCAAGCGTCAGTCGTTTCACCCCTCA
TTGTCAAGGACCAGTCAATA

Downstream 100 bases:

>100_bases
AAACAGCGGGCAGTTGCTGCTGATAGCGAGGCAGTATCTTCCTCTAAAGATACTGCGCTGCGAATGGGTCAGGCCTGACT
GCTGGAACTGGACCTCGTTC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSRNQQLFEQSQKFIPGGVNSPVRAFKSVGGTPVFFRKGEGAYAWDADDKSYIDYVGSWGPLILGHAHPEVVDAVYAAAK
NGLTFGAPTEAELEIAELLCRLVPSIEQVRLVSSGTEATMSAIRLARGYTARNRIIKFEGCYHGHDDALLVKAGSGALTF
GHPSSAGVPVETASSTVVLDYNDLAGVEQAFNQFGAEIAAVIVEPVAGNMNLIAPQPGFLAGLRELCTRHGSVLIFDEVM
TGFRVGLECAQGLYGIKPDLTTLGKVIGGGMPMAAFGGRREIMQCLAPVGAVYQAGTLSGNPVAVAAGLATLKLVQVPGF
YDKLAARTRKLTEGLAAAAAKQGVVFCAEAVGGMFGLYFRESAPKSYAEVMSCDREAFNGFFHAMLEEGIYFAPSAFEAG
FVSAAHGDAEISKTLATAEKIFARE

Sequences:

>Translated_425_residues
MSRNQQLFEQSQKFIPGGVNSPVRAFKSVGGTPVFFRKGEGAYAWDADDKSYIDYVGSWGPLILGHAHPEVVDAVYAAAK
NGLTFGAPTEAELEIAELLCRLVPSIEQVRLVSSGTEATMSAIRLARGYTARNRIIKFEGCYHGHDDALLVKAGSGALTF
GHPSSAGVPVETASSTVVLDYNDLAGVEQAFNQFGAEIAAVIVEPVAGNMNLIAPQPGFLAGLRELCTRHGSVLIFDEVM
TGFRVGLECAQGLYGIKPDLTTLGKVIGGGMPMAAFGGRREIMQCLAPVGAVYQAGTLSGNPVAVAAGLATLKLVQVPGF
YDKLAARTRKLTEGLAAAAAKQGVVFCAEAVGGMFGLYFRESAPKSYAEVMSCDREAFNGFFHAMLEEGIYFAPSAFEAG
FVSAAHGDAEISKTLATAEKIFARE
>Mature_424_residues
SRNQQLFEQSQKFIPGGVNSPVRAFKSVGGTPVFFRKGEGAYAWDADDKSYIDYVGSWGPLILGHAHPEVVDAVYAAAKN
GLTFGAPTEAELEIAELLCRLVPSIEQVRLVSSGTEATMSAIRLARGYTARNRIIKFEGCYHGHDDALLVKAGSGALTFG
HPSSAGVPVETASSTVVLDYNDLAGVEQAFNQFGAEIAAVIVEPVAGNMNLIAPQPGFLAGLRELCTRHGSVLIFDEVMT
GFRVGLECAQGLYGIKPDLTTLGKVIGGGMPMAAFGGRREIMQCLAPVGAVYQAGTLSGNPVAVAAGLATLKLVQVPGFY
DKLAARTRKLTEGLAAAAAKQGVVFCAEAVGGMFGLYFRESAPKSYAEVMSCDREAFNGFFHAMLEEGIYFAPSAFEAGF
VSAAHGDAEISKTLATAEKIFARE

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=330, Percent_Identity=30.3030303030303, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI24119277, Length=344, Percent_Identity=27.6162790697674, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI37574042, Length=347, Percent_Identity=28.2420749279539, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI284507298, Length=222, Percent_Identity=31.0810810810811, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI226442709, Length=316, Percent_Identity=29.4303797468354, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI226442705, Length=350, Percent_Identity=27.1428571428571, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=59.2941176470588, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789016, Length=379, Percent_Identity=33.245382585752, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789759, Length=307, Percent_Identity=30.6188925081433, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788044, Length=337, Percent_Identity=31.1572700296736, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1787560, Length=341, Percent_Identity=31.3782991202346, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693181, Length=297, Percent_Identity=30.976430976431, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786991, Length=364, Percent_Identity=28.8461538461538, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=377, Percent_Identity=27.0557029177719, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=355, Percent_Identity=26.1971830985915, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=187, Percent_Identity=28.3422459893048, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=321, Percent_Identity=28.3489096573209, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=324, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357415, Length=296, Percent_Identity=29.7297297297297, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_NITMU (Q2Y5Q5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413308.1
- ProteinModelPortal:   Q2Y5Q5
- SMR:   Q2Y5Q5
- STRING:   Q2Y5Q5
- GeneID:   3786332
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2629
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- PhylomeDB:   Q2Y5Q5
- ProtClustDB:   PRK00062
- BioCyc:   NMUL323848:NMUL_A2629-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 44871; Mature: 44740

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNQQLFEQSQKFIPGGVNSPVRAFKSVGGTPVFFRKGEGAYAWDADDKSYIDYVGSWG
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHCCCC
PLILGHAHPEVVDAVYAAAKNGLTFGAPTEAELEIAELLCRLVPSIEQVRLVSSGTEATM
CEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGYTARNRIIKFEGCYHGHDDALLVKAGSGALTFGHPSSAGVPVETASSTVVLD
HHHHHHHCCHHHCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCEECCCCEEEEE
YNDLAGVEQAFNQFGAEIAAVIVEPVAGNMNLIAPQPGFLAGLRELCTRHGSVLIFDEVM
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEHHHH
TGFRVGLECAQGLYGIKPDLTTLGKVIGGGMPMAAFGGRREIMQCLAPVGAVYQAGTLSG
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCC
NPVAVAAGLATLKLVQVPGFYDKLAARTRKLTEGLAAAAAKQGVVFCAEAVGGMFGLYFR
CCCEEEHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHH
ESAPKSYAEVMSCDREAFNGFFHAMLEEGIYFAPSAFEAGFVSAAHGDAEISKTLATAEK
HCCCHHHHHHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
IFARE
HHCCC
>Mature Secondary Structure 
SRNQQLFEQSQKFIPGGVNSPVRAFKSVGGTPVFFRKGEGAYAWDADDKSYIDYVGSWG
CCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHCCCC
PLILGHAHPEVVDAVYAAAKNGLTFGAPTEAELEIAELLCRLVPSIEQVRLVSSGTEATM
CEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGYTARNRIIKFEGCYHGHDDALLVKAGSGALTFGHPSSAGVPVETASSTVVLD
HHHHHHHCCHHHCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCEECCCCEEEEE
YNDLAGVEQAFNQFGAEIAAVIVEPVAGNMNLIAPQPGFLAGLRELCTRHGSVLIFDEVM
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEHHHH
TGFRVGLECAQGLYGIKPDLTTLGKVIGGGMPMAAFGGRREIMQCLAPVGAVYQAGTLSG
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCC
NPVAVAAGLATLKLVQVPGFYDKLAARTRKLTEGLAAAAAKQGVVFCAEAVGGMFGLYFR
CCCEEEHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHH
ESAPKSYAEVMSCDREAFNGFFHAMLEEGIYFAPSAFEAGFVSAAHGDAEISKTLATAEK
HCCCHHHHHHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
IFARE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA