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The map label for this gene is metK

Identifier: 82703656

GI number: 82703656

Start: 2909986

End: 2911185

Strand: Reverse

Name: metK

Synonym: Nmul_A2541

Alternate gene names: 82703656

Gene position: 2911185-2909986 (Counterclockwise)

Preceding gene: 82703662

Following gene: 82703655

Centisome position: 91.42

GC content: 55.83

Gene sequence:

>1200_bases
ATGAGCGAATATCTTTTTACCTCCGAATCCGTGTCTGAAGGTCATCCCGATAAGGTGGCCGATCAAATTTCCGATTCCAT
CCTCGACGCCATCCTGGCTCAGGATCCCAATGCACGGGTAGCTTGCGAAACGTTATGCAGTACCGGTCTGATCGTCATGT
CGGGCGAAATCACCACTCAGGCCAATGTGGACTACATGCAGGTCGCGCGCGCCGCGGTCAAGCGCATCGGTTACAACAGT
TCCGATATCGGCTTTGACTATAATACCTGTGCAGTGCTGACTGCTTTCAATAAGCAATCCCCTGATATCGCGCGCGGGGT
CAACCGCACCAAGGAAGAGGAAATGGATCAGGGGGCGGGTGACCAGGGTCTCATGTTCGGCTACGCCTGCGATGAGACGC
CGCAATTGATGCCCATGCCGATCTACTATGCCCACCGGTTGATGGAGCGCCAGGCGGAACTGCGGAAAGACGGACGCCTC
CCGTGGCTGCGTCCGGATGCAAAATCGCAGGTTTCAGTACGTTACCTGGACGGCAAGCCGCAGCGAATCGAGACCGTTGT
CATTTCCACACAACATCACCCGGACATCAGTCACACCGATTTGAGCGAGGCCATTATTGAGGAAGTCATCAAACCCGTGC
TTCCGAAAAAAATGCTGAGTGGCGAAACGCGGTATCTGATCAACCCCACAGGACGTTTCGTAGTGGGCGGCCCCATGGGA
GATTGCGGACTGACCGGACGGAAGATTATCGTCGACAGCTATGGAGGTACCGCGCACCATGGCGGGGGCGCCTTCTCCGG
CAAGGATCCGTCCAAAGTAGATCGTTCCGCTGCTTATGCCGGACGATATGTGGCAAAGAATCTTGTTGCGGCCGGTATCG
CCAGTAGATGCGAGGTACAGATGGCTTATGCGATCGGAGTTGCCCGCCCCGTTTCGCTGATGGTGGACACTTTTGGAACC
GGAAAAATTCCGGACGATAAAATCGTCAAACTGATTGAGCGGCACTTCGATCTGCGTCCGCGCGGCATCATCCACGGCCT
TGATCTGCTGCGTCCCATCTACGAGAAGACAGCTGCCTATGGCCATTTTGGCCGGGACGAACCGGAATTCAGCTGGGAAT
CGACGGATAAGGCTGCACAGTTGCGCGAGGAAGCGGGAATTGAACCCGCGGAAACCGAACCGCTCAGTCTTCAGGCTTAA

Upstream 100 bases:

>100_bases
GAAACATCGGCATGTCAAGCCGTATTGTATTATCCGTCTTCTTAATAAGCGTAGAATTTGGTATTCTACAGACCTTTTAC
GATTAAGCGGGCTGGACAAA

Downstream 100 bases:

>100_bases
GATCACATCACCCACCCTTCCAGACCGAGGAGCGCTGCAACGGGCATTGTCCCGCGAGGCTCGGCAAGGAAGGATCGCTA
CAACGGCGCTCGTTCATTTT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSEYLFTSESVSEGHPDKVADQISDSILDAILAQDPNARVACETLCSTGLIVMSGEITTQANVDYMQVARAAVKRIGYNS
SDIGFDYNTCAVLTAFNKQSPDIARGVNRTKEEEMDQGAGDQGLMFGYACDETPQLMPMPIYYAHRLMERQAELRKDGRL
PWLRPDAKSQVSVRYLDGKPQRIETVVISTQHHPDISHTDLSEAIIEEVIKPVLPKKMLSGETRYLINPTGRFVVGGPMG
DCGLTGRKIIVDSYGGTAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNLVAAGIASRCEVQMAYAIGVARPVSLMVDTFGT
GKIPDDKIVKLIERHFDLRPRGIIHGLDLLRPIYEKTAAYGHFGRDEPEFSWESTDKAAQLREEAGIEPAETEPLSLQA

Sequences:

>Translated_399_residues
MSEYLFTSESVSEGHPDKVADQISDSILDAILAQDPNARVACETLCSTGLIVMSGEITTQANVDYMQVARAAVKRIGYNS
SDIGFDYNTCAVLTAFNKQSPDIARGVNRTKEEEMDQGAGDQGLMFGYACDETPQLMPMPIYYAHRLMERQAELRKDGRL
PWLRPDAKSQVSVRYLDGKPQRIETVVISTQHHPDISHTDLSEAIIEEVIKPVLPKKMLSGETRYLINPTGRFVVGGPMG
DCGLTGRKIIVDSYGGTAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNLVAAGIASRCEVQMAYAIGVARPVSLMVDTFGT
GKIPDDKIVKLIERHFDLRPRGIIHGLDLLRPIYEKTAAYGHFGRDEPEFSWESTDKAAQLREEAGIEPAETEPLSLQA
>Mature_398_residues
SEYLFTSESVSEGHPDKVADQISDSILDAILAQDPNARVACETLCSTGLIVMSGEITTQANVDYMQVARAAVKRIGYNSS
DIGFDYNTCAVLTAFNKQSPDIARGVNRTKEEEMDQGAGDQGLMFGYACDETPQLMPMPIYYAHRLMERQAELRKDGRLP
WLRPDAKSQVSVRYLDGKPQRIETVVISTQHHPDISHTDLSEAIIEEVIKPVLPKKMLSGETRYLINPTGRFVVGGPMGD
CGLTGRKIIVDSYGGTAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNLVAAGIASRCEVQMAYAIGVARPVSLMVDTFGTG
KIPDDKIVKLIERHFDLRPRGIIHGLDLLRPIYEKTAAYGHFGRDEPEFSWESTDKAAQLREEAGIEPAETEPLSLQA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=56.8783068783069, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=57.2192513368984, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=65.7216494845361, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17538494, Length=375, Percent_Identity=57.6, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538498, Length=375, Percent_Identity=57.6, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=57.6315789473684, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551082, Length=376, Percent_Identity=56.3829787234042, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32565905, Length=375, Percent_Identity=52.2666666666667, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=55.1829268292683, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=55.421686746988, Blast_Score=357, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=55.4817275747508, Blast_Score=323, Evalue=9e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=61.4457831325301, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=77.5, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=378, Percent_Identity=58.2010582010582, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6323209, Length=374, Percent_Identity=57.7540106951872, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=55.6149732620321, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=55.6149732620321, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=55.6149732620321, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=55.6149732620321, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=55.6149732620321, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=55.6149732620321, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=55.6149732620321, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=55.6149732620321, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=53.3333333333333, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=61.3445378151261, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_NITMU (Q2Y5Z1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413222.1
- ProteinModelPortal:   Q2Y5Z1
- SMR:   Q2Y5Z1
- STRING:   Q2Y5Z1
- GeneID:   3784046
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2541
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q2Y5Z1
- ProtClustDB:   PRK05250
- BioCyc:   NMUL323848:NMUL_A2541-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43710; Mature: 43579

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYLFTSESVSEGHPDKVADQISDSILDAILAQDPNARVACETLCSTGLIVMSGEITTQ
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCC
ANVDYMQVARAAVKRIGYNSSDIGFDYNTCAVLTAFNKQSPDIARGVNRTKEEEMDQGAG
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHCCCC
DQGLMFGYACDETPQLMPMPIYYAHRLMERQAELRKDGRLPWLRPDAKSQVSVRYLDGKP
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
QRIETVVISTQHHPDISHTDLSEAIIEEVIKPVLPKKMLSGETRYLINPTGRFVVGGPMG
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDSYGGTAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNLVAAGIASRCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
MAYAIGVARPVSLMVDTFGTGKIPDDKIVKLIERHFDLRPRGIIHGLDLLRPIYEKTAAY
EHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GHFGRDEPEFSWESTDKAAQLREEAGIEPAETEPLSLQA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEYLFTSESVSEGHPDKVADQISDSILDAILAQDPNARVACETLCSTGLIVMSGEITTQ
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCC
ANVDYMQVARAAVKRIGYNSSDIGFDYNTCAVLTAFNKQSPDIARGVNRTKEEEMDQGAG
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHCCCC
DQGLMFGYACDETPQLMPMPIYYAHRLMERQAELRKDGRLPWLRPDAKSQVSVRYLDGKP
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
QRIETVVISTQHHPDISHTDLSEAIIEEVIKPVLPKKMLSGETRYLINPTGRFVVGGPMG
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDSYGGTAHHGGGAFSGKDPSKVDRSAAYAGRYVAKNLVAAGIASRCEVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
MAYAIGVARPVSLMVDTFGTGKIPDDKIVKLIERHFDLRPRGIIHGLDLLRPIYEKTAAY
EHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GHFGRDEPEFSWESTDKAAQLREEAGIEPAETEPLSLQA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA