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The map label for this gene is metF [H]

Identifier: 82703654

GI number: 82703654

Start: 2907499

End: 2908344

Strand: Reverse

Name: metF [H]

Synonym: Nmul_A2539

Alternate gene names: 82703654

Gene position: 2908344-2907499 (Counterclockwise)

Preceding gene: 82703655

Following gene: 82703649

Centisome position: 91.34

GC content: 54.14

Gene sequence:

>846_bases
ATGGAATCTCAAAAAAAATTTGTTCCCACATTCAGCTTCGAGTTTTTTCCGCCGCAGACTGCGGAAGGAATAGAGAAGCT
GCGCGCTACGCGCAGTCAACTTGCGCAACTTAAGCCGAAATTCTTCTCGGTAACCTTCGGCGCGGGAGGATCGACACGCG
ATCGTACCCTGGAGACTGTGCTCGAGATTCAGGCAGAGGGCCTCATCGCTGCTCCCCATCTTTCCTGTATTGGCGCAACC
CGCGAAAACATCCGCGCCATGCTGGAACAGTACCGCAGCAGCGGCATTCGCCATCTGATTGCGCTACGGGGGGACCTGCC
TTCGGGAATGGCGCAAGGAGGAGAGTTCCGCTATGCCAACGAACTGGTAGCCTTTATTCGCCGCGAGTACGGCAACGCCT
TCCATATCGATGTGGCGTCCTATCCCGAGTACCATCCACAAGCCAGAACTGCTCAGGAAGACTTGAGGAATTTCAAGCGT
AAAATCGAAGCCGGAGCGGACTCGGCCATTACCCAGTATTTTTACAACGCGGATGCCTATTTTGACTTCGTCGATTCATG
TGAGGCGATGGGCATCAATATTCCGGTGGTTCCCGGCATCATGCCAATCAATAGATTTTCCCAGCTTGCCCGATTCTCGG
ATACCTGCGGGGCGGAAATACCGCGCTGGATCAGGAAGCGGCTGGAAGGTTATGGAGATGACACTGCTTCCATACGCGCC
TTCGGCCTGGATGTCGTTACCGATCTGTGCGCTCGACTACTGAATGCCGGTGCACCCGGATTGCATTTCTATACGTTGAA
CTCGGCAGGACTAACGACAACTGTTTGGCAAAGATTGGGGCTGTAG

Upstream 100 bases:

>100_bases
GTACCGTTATTGATTTTTTTCAGGTCTGGATTAGCGCGGTGAAGGAAAGAGGGGATTAGAACACCATCTTTTTACCCTCT
CCGCGCTTTTCCGCTTTGCC

Downstream 100 bases:

>100_bases
TTCACCCCATTTTCAGATCGAATGCTCACGAGAAGCGGGTGAGCGGGCAACGAAGACCGGGCGGCCAGCACGGTGATGGG
GGAGCGAAGACGGCGAACAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MESQKKFVPTFSFEFFPPQTAEGIEKLRATRSQLAQLKPKFFSVTFGAGGSTRDRTLETVLEIQAEGLIAAPHLSCIGAT
RENIRAMLEQYRSSGIRHLIALRGDLPSGMAQGGEFRYANELVAFIRREYGNAFHIDVASYPEYHPQARTAQEDLRNFKR
KIEAGADSAITQYFYNADAYFDFVDSCEAMGINIPVVPGIMPINRFSQLARFSDTCGAEIPRWIRKRLEGYGDDTASIRA
FGLDVVTDLCARLLNAGAPGLHFYTLNSAGLTTTVWQRLGL

Sequences:

>Translated_281_residues
MESQKKFVPTFSFEFFPPQTAEGIEKLRATRSQLAQLKPKFFSVTFGAGGSTRDRTLETVLEIQAEGLIAAPHLSCIGAT
RENIRAMLEQYRSSGIRHLIALRGDLPSGMAQGGEFRYANELVAFIRREYGNAFHIDVASYPEYHPQARTAQEDLRNFKR
KIEAGADSAITQYFYNADAYFDFVDSCEAMGINIPVVPGIMPINRFSQLARFSDTCGAEIPRWIRKRLEGYGDDTASIRA
FGLDVVTDLCARLLNAGAPGLHFYTLNSAGLTTTVWQRLGL
>Mature_281_residues
MESQKKFVPTFSFEFFPPQTAEGIEKLRATRSQLAQLKPKFFSVTFGAGGSTRDRTLETVLEIQAEGLIAAPHLSCIGAT
RENIRAMLEQYRSSGIRHLIALRGDLPSGMAQGGEFRYANELVAFIRREYGNAFHIDVASYPEYHPQARTAQEDLRNFKR
KIEAGADSAITQYFYNADAYFDFVDSCEAMGINIPVVPGIMPINRFSQLARFSDTCGAEIPRWIRKRLEGYGDDTASIRA
FGLDVVTDLCARLLNAGAPGLHFYTLNSAGLTTTVWQRLGL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=290, Percent_Identity=39.3103448275862, Blast_Score=198, Evalue=4e-51,
Organism=Escherichia coli, GI1790377, Length=271, Percent_Identity=42.8044280442804, Blast_Score=238, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI25147441, Length=285, Percent_Identity=34.0350877192982, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25147436, Length=285, Percent_Identity=34.0350877192982, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321313, Length=285, Percent_Identity=34.3859649122807, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6325234, Length=278, Percent_Identity=35.6115107913669, Blast_Score=157, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24662658, Length=222, Percent_Identity=28.8288288288288, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31309; Mature: 31309

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQKKFVPTFSFEFFPPQTAEGIEKLRATRSQLAQLKPKFFSVTFGAGGSTRDRTLETV
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
LEIQAEGLIAAPHLSCIGATRENIRAMLEQYRSSGIRHLIALRGDLPSGMAQGGEFRYAN
HHHHHCCEEECCCHHHHHCCHHHHHHHHHHHHHCCHHEEHHHCCCCCHHHHCCCCCHHHH
ELVAFIRREYGNAFHIDVASYPEYHPQARTAQEDLRNFKRKIEAGADSAITQYFYNADAY
HHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHH
FDFVDSCEAMGINIPVVPGIMPINRFSQLARFSDTCGAEIPRWIRKRLEGYGDDTASIRA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHH
FGLDVVTDLCARLLNAGAPGLHFYTLNSAGLTTTVWQRLGL
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MESQKKFVPTFSFEFFPPQTAEGIEKLRATRSQLAQLKPKFFSVTFGAGGSTRDRTLETV
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
LEIQAEGLIAAPHLSCIGATRENIRAMLEQYRSSGIRHLIALRGDLPSGMAQGGEFRYAN
HHHHHCCEEECCCHHHHHCCHHHHHHHHHHHHHCCHHEEHHHCCCCCHHHHCCCCCHHHH
ELVAFIRREYGNAFHIDVASYPEYHPQARTAQEDLRNFKRKIEAGADSAITQYFYNADAY
HHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHH
FDFVDSCEAMGINIPVVPGIMPINRFSQLARFSDTCGAEIPRWIRKRLEGYGDDTASIRA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHH
FGLDVVTDLCARLLNAGAPGLHFYTLNSAGLTTTVWQRLGL
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]