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The map label for this gene is nadE [H]

Identifier: 82703650

GI number: 82703650

Start: 2901974

End: 2903590

Strand: Direct

Name: nadE [H]

Synonym: Nmul_A2535

Alternate gene names: 82703650

Gene position: 2901974-2903590 (Clockwise)

Preceding gene: 82703647

Following gene: 82703651

Centisome position: 91.14

GC content: 53.12

Gene sequence:

>1617_bases
ATGAAGCTCGCCATTGCTCAGATCAATTGTACAGTCGGCGACCTCGGAGGCAATACCCGAAAGATCCTCGATTACGCCAA
TCAGGCAAAAAATGCGGGAGCCAGACTGGTCGTAACCCCCGAACTCGCCTTGTCCGGATACCCCCCCGAAGACCTGTTAT
TGCGCCACGGATTCCGGCATGCATGCCAGAATGCGCTTACAGAGTTGGCTGGCAAGACAGGCGATATCACCGTTGTAGTC
GGACATCCTCATCTTGCCGCGGATAAACTGTACAACGCTGCATCGGTAATACGCAACGGTAAAATCATCGCTACGTATCT
GAAAAATCTTTTGCCTAACGATAGCGTATTCGATGAACGACGTTACTTCGAACCGGGCTTCCGACCCTGCGTGTTCGAAC
TGGATGGAATCAGATTCGCTATCAACATTTGTCAGGACATCTGGCAGGAAGGTTCCGCCACACGTGCAAAAGAAGGCGGG
GCGGAGGTGCTGCTGGTGCTGAATGCTTCCCCTTATCACATGAACAAACAGGCGTTGCGCTACGATCTGGCTCGGAAGCG
TATCGATGAAACGGGGCTGTCGATCGTATATGCCAATCTTATTGGAGGCCAGGATGAACTAATATTCGATGGTGCATCTT
TCGCCATGAACGCCAGAGGCGACGTGACTCATCAGTTCGATGCGCTCGTGGAAGCACTCGGCATGGTCGAATTGGAGGGG
GCTGCGCCCGTAACGACAGAAATCGCACCGCTTGAAAGTCTGGAAGCGAACGTTTATAAAGCGCTCTGTCTCGGAGTAAA
GGACTATATAGGAAAAAACCGCATTCCTGGCGTATTGCTGGGGCTATCAGGGGGCGTGGATTCGGCGCTCACCATGACGA
TTGCGGCGGATGCCCTGGGTGCAGACAAAGTAAAAGCAGTGATGATGCCCTCACGGTTCACTGCCGACATGAGCTTGCTG
GATGCCCGCACTATGGCGCAAACATTGGGGGTGCGCTATACCGAATTCCCAATTGAACCGGTATTTGGAGAATTCAAGGA
TACGCTTGCCAAAGAATTTGCCGCGTTGCCCCCTTCTGACGTCCCGGATCTTACCGAGGAAAATCTGCAAGCCCGGATAC
GCGGAACTTTGCTGATGGCGTTATCCAATAAATCCGGCGCCATCGTCCTCATCACGGGCAATAAATCCGAAACAGCCGTA
GGCTACTCCACTCTATATGGGGATACGGCAGGGGGCTTTGCCGTCTTGAAAGATGTCACCAAAACCATGGTGTACAAGTT
ATGCCAGTACCGTAACGGGGTAGGAAAAGTTATTCCGGACCGTGTTCTCAGTCGCGCCCCTTCAGCGGAATTGCGTCCCG
ATCAGACTGACCAGGATAGTCTTCCGCCGTATGCAGTTCTGGATGCTATTCTGGAAGAATATGTGGAACATGATATGTCG
CTTGAGGAAATTCTGCGCATGGGCTACGACGAAAGGGATGCGCGCCGCGTCGTGCAACTGATCCGGCAGAATGAATACAA
GCGGCGGCAGTCTCCACCCGGCATTCGCGTGACCTCCCGCGCATTCGGCAAGGACTGGCGTTATCCTATAACAGCGCGGT
ATCAGGACCAGTTTTAG

Upstream 100 bases:

>100_bases
ATTTGGCAATTCCAGCGCTTTTTCCGCCTGGGACTCGTCCCCGGAGTTCGTTCTGGTATCATGTAAGCATAAAAACAGAC
CAGCCCAATTGACCGTCCAG

Downstream 100 bases:

>100_bases
GAGCGATCTGGAAGAAGTGCGCAAAAGCCATGTCAGCGGATTAAAATGGCGGTATGCTGCGAATACCGGAAGCCGCGAGG
AAAAGGCCTCTTATGGCTTT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MKLAIAQINCTVGDLGGNTRKILDYANQAKNAGARLVVTPELALSGYPPEDLLLRHGFRHACQNALTELAGKTGDITVVV
GHPHLAADKLYNAASVIRNGKIIATYLKNLLPNDSVFDERRYFEPGFRPCVFELDGIRFAINICQDIWQEGSATRAKEGG
AEVLLVLNASPYHMNKQALRYDLARKRIDETGLSIVYANLIGGQDELIFDGASFAMNARGDVTHQFDALVEALGMVELEG
AAPVTTEIAPLESLEANVYKALCLGVKDYIGKNRIPGVLLGLSGGVDSALTMTIAADALGADKVKAVMMPSRFTADMSLL
DARTMAQTLGVRYTEFPIEPVFGEFKDTLAKEFAALPPSDVPDLTEENLQARIRGTLLMALSNKSGAIVLITGNKSETAV
GYSTLYGDTAGGFAVLKDVTKTMVYKLCQYRNGVGKVIPDRVLSRAPSAELRPDQTDQDSLPPYAVLDAILEEYVEHDMS
LEEILRMGYDERDARRVVQLIRQNEYKRRQSPPGIRVTSRAFGKDWRYPITARYQDQF

Sequences:

>Translated_538_residues
MKLAIAQINCTVGDLGGNTRKILDYANQAKNAGARLVVTPELALSGYPPEDLLLRHGFRHACQNALTELAGKTGDITVVV
GHPHLAADKLYNAASVIRNGKIIATYLKNLLPNDSVFDERRYFEPGFRPCVFELDGIRFAINICQDIWQEGSATRAKEGG
AEVLLVLNASPYHMNKQALRYDLARKRIDETGLSIVYANLIGGQDELIFDGASFAMNARGDVTHQFDALVEALGMVELEG
AAPVTTEIAPLESLEANVYKALCLGVKDYIGKNRIPGVLLGLSGGVDSALTMTIAADALGADKVKAVMMPSRFTADMSLL
DARTMAQTLGVRYTEFPIEPVFGEFKDTLAKEFAALPPSDVPDLTEENLQARIRGTLLMALSNKSGAIVLITGNKSETAV
GYSTLYGDTAGGFAVLKDVTKTMVYKLCQYRNGVGKVIPDRVLSRAPSAELRPDQTDQDSLPPYAVLDAILEEYVEHDMS
LEEILRMGYDERDARRVVQLIRQNEYKRRQSPPGIRVTSRAFGKDWRYPITARYQDQF
>Mature_538_residues
MKLAIAQINCTVGDLGGNTRKILDYANQAKNAGARLVVTPELALSGYPPEDLLLRHGFRHACQNALTELAGKTGDITVVV
GHPHLAADKLYNAASVIRNGKIIATYLKNLLPNDSVFDERRYFEPGFRPCVFELDGIRFAINICQDIWQEGSATRAKEGG
AEVLLVLNASPYHMNKQALRYDLARKRIDETGLSIVYANLIGGQDELIFDGASFAMNARGDVTHQFDALVEALGMVELEG
AAPVTTEIAPLESLEANVYKALCLGVKDYIGKNRIPGVLLGLSGGVDSALTMTIAADALGADKVKAVMMPSRFTADMSLL
DARTMAQTLGVRYTEFPIEPVFGEFKDTLAKEFAALPPSDVPDLTEENLQARIRGTLLMALSNKSGAIVLITGNKSETAV
GYSTLYGDTAGGFAVLKDVTKTMVYKLCQYRNGVGKVIPDRVLSRAPSAELRPDQTDQDSLPPYAVLDAILEEYVEHDMS
LEEILRMGYDERDARRVVQLIRQNEYKRRQSPPGIRVTSRAFGKDWRYPITARYQDQF

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59017; Mature: 59017

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAIAQINCTVGDLGGNTRKILDYANQAKNAGARLVVTPELALSGYPPEDLLLRHGFRH
CEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCHHHHHHHHHHH
ACQNALTELAGKTGDITVVVGHPHLAADKLYNAASVIRNGKIIATYLKNLLPNDSVFDER
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHH
RYFEPGFRPCVFELDGIRFAINICQDIWQEGSATRAKEGGAEVLLVLNASPYHMNKQALR
HHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHH
YDLARKRIDETGLSIVYANLIGGQDELIFDGASFAMNARGDVTHQFDALVEALGMVELEG
HHHHHHHHHHCCCEEEEEECCCCCCCEEECCCHHEECCCCCCHHHHHHHHHHHCCEEECC
AAPVTTEIAPLESLEANVYKALCLGVKDYIGKNRIPGVLLGLSGGVDSALTMTIAADALG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEHHHHHCC
ADKVKAVMMPSRFTADMSLLDARTMAQTLGVRYTEFPIEPVFGEFKDTLAKEFAALPPSD
CHHHHEEECCCHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCC
VPDLTEENLQARIRGTLLMALSNKSGAIVLITGNKSETAVGYSTLYGDTAGGFAVLKDVT
CCCCCHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCEECHHHHCCCCCCHHHHHHHHH
KTMVYKLCQYRNGVGKVIPDRVLSRAPSAELRPDQTDQDSLPPYAVLDAILEEYVEHDMS
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LEEILRMGYDERDARRVVQLIRQNEYKRRQSPPGIRVTSRAFGKDWRYPITARYQDQF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MKLAIAQINCTVGDLGGNTRKILDYANQAKNAGARLVVTPELALSGYPPEDLLLRHGFRH
CEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCHHHHHHHHHHH
ACQNALTELAGKTGDITVVVGHPHLAADKLYNAASVIRNGKIIATYLKNLLPNDSVFDER
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHH
RYFEPGFRPCVFELDGIRFAINICQDIWQEGSATRAKEGGAEVLLVLNASPYHMNKQALR
HHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHH
YDLARKRIDETGLSIVYANLIGGQDELIFDGASFAMNARGDVTHQFDALVEALGMVELEG
HHHHHHHHHHCCCEEEEEECCCCCCCEEECCCHHEECCCCCCHHHHHHHHHHHCCEEECC
AAPVTTEIAPLESLEANVYKALCLGVKDYIGKNRIPGVLLGLSGGVDSALTMTIAADALG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEHHHHHCC
ADKVKAVMMPSRFTADMSLLDARTMAQTLGVRYTEFPIEPVFGEFKDTLAKEFAALPPSD
CHHHHEEECCCHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCC
VPDLTEENLQARIRGTLLMALSNKSGAIVLITGNKSETAVGYSTLYGDTAGGFAVLKDVT
CCCCCHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCEECHHHHCCCCCCHHHHHHHHH
KTMVYKLCQYRNGVGKVIPDRVLSRAPSAELRPDQTDQDSLPPYAVLDAILEEYVEHDMS
HHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LEEILRMGYDERDARRVVQLIRQNEYKRRQSPPGIRVTSRAFGKDWRYPITARYQDQF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]