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The map label for this gene is amiC [H]

Identifier: 82703647

GI number: 82703647

Start: 2898467

End: 2899837

Strand: Direct

Name: amiC [H]

Synonym: Nmul_A2532

Alternate gene names: 82703647

Gene position: 2898467-2899837 (Clockwise)

Preceding gene: 82703646

Following gene: 82703650

Centisome position: 91.03

GC content: 55.0

Gene sequence:

>1371_bases
TTGAAGAGGGAGCGCTCACAGTCTGTTCCTTCAGTCTCACAATCCTTGCGCTCAACCCGTTTCTCCTCCATCCGGAACCG
CCTGGCAGCTGGCGCATGCCTCTCGATCACGGCACTCCTGTTATCGTCATCCGCCAGCTCTGTCTGTGCTCAAGAAAAGA
TACGCATCAGTTCCGCCCGTATCTGGCCATCCGAGGAATACACCCGTCTTACGCTTGAATCCGCTTCCGCCATTCGCTAC
ACCCTCACTACGGTAAAAGATCCGGACCGCGTCGTCGTTGACCTTGAGGATGTCTCACTGACACCGGAGCTGAAAAGGCT
GGCAGACAGGATAGATCCTGCAGATCCGCACATTCACGCCGTGCGTTTCGGTCATTTCAAACCAGACATACTGCGGCTGG
TGCTTGACCTGAAAACCGAAGTCGAACCCCAGGCATTTGTCATTCAACCCGTTGGAACGTACGGACATCGACTTGTTCTG
GACATCTACCCTGCTATTCCACCCGATCCGCTCATGGCCTTTCTGAATAACAAGGACAAAGCAGGATACATGCTGCGGGA
TGAACCCCACAATGGGACTCATGTCAGTTCAAGGACTGAAATCGAGACTGTCCGGAGCAATGAAGCGGCAAGAAAAAACA
GGCCGGAAATGATTCGTCTCATCACTGTCGCCATTGATCCCGGACATGGTGGGGAGGACCCGGGCGCGATAAGCCGGCGC
GGTACCAGGGAGAAGAACATCACTCTGGCCATCGCCAGGAAGCTGAAGGAAAAGATAGATAGGGAGCCGAATATGCGCGC
GGCTCTCACCCGTGACGGAGATTATTATATTTCACTGTCGCAGCGGCTGGTAAAAGCACGTCAGCTGAATGCGGATCTGT
TCGTATCGGTGCACGCGGACGCCTTCAACAAGCCCCATGCCCGCGGCTCCTCGGTGTTTGCCCTTTCAGAACGGGGCGCC
ACTTCGGCGGCTGCGGGATGGCTGGCAAGAAAAGAAAACGAGGCGGACCTTGTCGGGGGCGTCAACCTCGATATCCCGGA
CCCTTTTCTCAAGCGGACTTTGCTGGATTTGTCCCAGACGGCAACGATCAACGACAGCCTCAAACTCGGCAAGGCAGTCC
TCTCCAAGATAGGCAATATCAATCATCTGCACAAGGCCGAAGTCGAGCAGGCAGGCTTTGCCGTCCTCAAGTCTCCCGAC
ATTCCATCGATCCTGGTCGAGACGGCATTCATCAGCAATCCGGATGAGGAAAGGAAACTGAGAGACCACGGTTATCAGGA
CAAGCTGGCCTCCGCAATACTTGCGGGTATCAAACATTACTTCGCTGACAACCCTCCCCTCTCGCGTTCGAGAATGGTTT
CCCTCGATTAG

Upstream 100 bases:

>100_bases
AGGTCGCAGAAGGGCGTGATGTTGAAATTCAGGCGGATACGGAGGCCGGCAAACTGTGCGTGAAACGATGGCAAATCTCA
TCGAGGAACAATATGCTGGG

Downstream 100 bases:

>100_bases
CGAATTAGCCTCCGCCTTTTCTTTCCGGGGTTTCTCGCAGTCTCTCCCGCTGCTGCCCGCACCGTTTTGTGCTTCTGGCT
CACCAGCATTTGATTGTCCG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKRERSQSVPSVSQSLRSTRFSSIRNRLAAGACLSITALLLSSSASSVCAQEKIRISSARIWPSEEYTRLTLESASAIRY
TLTTVKDPDRVVVDLEDVSLTPELKRLADRIDPADPHIHAVRFGHFKPDILRLVLDLKTEVEPQAFVIQPVGTYGHRLVL
DIYPAIPPDPLMAFLNNKDKAGYMLRDEPHNGTHVSSRTEIETVRSNEAARKNRPEMIRLITVAIDPGHGGEDPGAISRR
GTREKNITLAIARKLKEKIDREPNMRAALTRDGDYYISLSQRLVKARQLNADLFVSVHADAFNKPHARGSSVFALSERGA
TSAAAGWLARKENEADLVGGVNLDIPDPFLKRTLLDLSQTATINDSLKLGKAVLSKIGNINHLHKAEVEQAGFAVLKSPD
IPSILVETAFISNPDEERKLRDHGYQDKLASAILAGIKHYFADNPPLSRSRMVSLD

Sequences:

>Translated_456_residues
MKRERSQSVPSVSQSLRSTRFSSIRNRLAAGACLSITALLLSSSASSVCAQEKIRISSARIWPSEEYTRLTLESASAIRY
TLTTVKDPDRVVVDLEDVSLTPELKRLADRIDPADPHIHAVRFGHFKPDILRLVLDLKTEVEPQAFVIQPVGTYGHRLVL
DIYPAIPPDPLMAFLNNKDKAGYMLRDEPHNGTHVSSRTEIETVRSNEAARKNRPEMIRLITVAIDPGHGGEDPGAISRR
GTREKNITLAIARKLKEKIDREPNMRAALTRDGDYYISLSQRLVKARQLNADLFVSVHADAFNKPHARGSSVFALSERGA
TSAAAGWLARKENEADLVGGVNLDIPDPFLKRTLLDLSQTATINDSLKLGKAVLSKIGNINHLHKAEVEQAGFAVLKSPD
IPSILVETAFISNPDEERKLRDHGYQDKLASAILAGIKHYFADNPPLSRSRMVSLD
>Mature_456_residues
MKRERSQSVPSVSQSLRSTRFSSIRNRLAAGACLSITALLLSSSASSVCAQEKIRISSARIWPSEEYTRLTLESASAIRY
TLTTVKDPDRVVVDLEDVSLTPELKRLADRIDPADPHIHAVRFGHFKPDILRLVLDLKTEVEPQAFVIQPVGTYGHRLVL
DIYPAIPPDPLMAFLNNKDKAGYMLRDEPHNGTHVSSRTEIETVRSNEAARKNRPEMIRLITVAIDPGHGGEDPGAISRR
GTREKNITLAIARKLKEKIDREPNMRAALTRDGDYYISLSQRLVKARQLNADLFVSVHADAFNKPHARGSSVFALSERGA
TSAAAGWLARKENEADLVGGVNLDIPDPFLKRTLLDLSQTATINDSLKLGKAVLSKIGNINHLHKAEVEQAGFAVLKSPD
IPSILVETAFISNPDEERKLRDHGYQDKLASAILAGIKHYFADNPPLSRSRMVSLD

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=428, Percent_Identity=46.7289719626168, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1788776, Length=219, Percent_Identity=50.2283105022831, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1790611, Length=230, Percent_Identity=46.0869565217391, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 50403; Mature: 50403

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRERSQSVPSVSQSLRSTRFSSIRNRLAAGACLSITALLLSSSASSVCAQEKIRISSAR
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCEE
IWPSEEYTRLTLESASAIRYTLTTVKDPDRVVVDLEDVSLTPELKRLADRIDPADPHIHA
CCCCCCCEEEEECCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEE
VRFGHFKPDILRLVLDLKTEVEPQAFVIQPVGTYGHRLVLDIYPAIPPDPLMAFLNNKDK
EEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCCCC
AGYMLRDEPHNGTHVSSRTEIETVRSNEAARKNRPEMIRLITVAIDPGHGGEDPGAISRR
CCCEEECCCCCCCCCCCCCHHHHHHCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
GTREKNITLAIARKLKEKIDREPNMRAALTRDGDYYISLSQRLVKARQLNADLFVSVHAD
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEEECC
AFNKPHARGSSVFALSERGATSAAAGWLARKENEADLVGGVNLDIPDPFLKRTLLDLSQT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHC
ATINDSLKLGKAVLSKIGNINHLHKAEVEQAGFAVLKSPDIPSILVETAFISNPDEERKL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCHHHHH
RDHGYQDKLASAILAGIKHYFADNPPLSRSRMVSLD
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
>Mature Secondary Structure
MKRERSQSVPSVSQSLRSTRFSSIRNRLAAGACLSITALLLSSSASSVCAQEKIRISSAR
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCEE
IWPSEEYTRLTLESASAIRYTLTTVKDPDRVVVDLEDVSLTPELKRLADRIDPADPHIHA
CCCCCCCEEEEECCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEE
VRFGHFKPDILRLVLDLKTEVEPQAFVIQPVGTYGHRLVLDIYPAIPPDPLMAFLNNKDK
EEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCCCC
AGYMLRDEPHNGTHVSSRTEIETVRSNEAARKNRPEMIRLITVAIDPGHGGEDPGAISRR
CCCEEECCCCCCCCCCCCCHHHHHHCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCC
GTREKNITLAIARKLKEKIDREPNMRAALTRDGDYYISLSQRLVKARQLNADLFVSVHAD
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEEECC
AFNKPHARGSSVFALSERGATSAAAGWLARKENEADLVGGVNLDIPDPFLKRTLLDLSQT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHC
ATINDSLKLGKAVLSKIGNINHLHKAEVEQAGFAVLKSPDIPSILVETAFISNPDEERKL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCHHHHH
RDHGYQDKLASAILAGIKHYFADNPPLSRSRMVSLD
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]