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The map label for this gene is relA [H]

Identifier: 82703620

GI number: 82703620

Start: 2861406

End: 2863595

Strand: Reverse

Name: relA [H]

Synonym: Nmul_A2505

Alternate gene names: 82703620

Gene position: 2863595-2861406 (Counterclockwise)

Preceding gene: 82703621

Following gene: 82703618

Centisome position: 89.93

GC content: 55.39

Gene sequence:

>2190_bases
ATGAGCGCTCCCTCCCTCATTTCCGGAAAAGGGGACGGACCTTTCCGGCTCCATTCCCCGGGTTCGATCTTCTCCCCGAC
AGAAATCGATCTGCTCCGGGAGGCACAGGTTTTTTGTGAACCCCTGTATATGGATAAAAGCCTGCCTAACGGTGAATTGC
TCTCTTCGCATGTAGATGGACTCGCGGCGATTCTCGCGGGACTGCGCGTGGACAGCGATACACTGGCAGCAGGAGTGCTT
CATGCAGTTCCGGAGTATCTGGAGGGGTATGAAGAAGAATTGGCAACCGCTTTTGGTCCAATAGTCGCTCATCTCGTGGA
AGGTGTGAGGCGCGTCGGCCGAATCCAGGTGCTGCGATCTGCAAACAGCACAGACAATATCGGGAATGGCAGTGCTCAGA
TTGAAGCATTGAGGAAGATGCTGCTCGCAATGGCGGAAGACATCCGCGTGGTGATCATCGCCCTGGCCGACCGGTTGCAG
ACGATGCGCTACGTTGCTGCCCGCAACGTTGCCGGAAGATGGGAGGCCGCTGAGGTCACTTTGAATATTTTCGCGCCTCT
CGCCAATCGCCTGGGGTTATGGCAAGTGAAGTGGGAACTGGAGGATCTATCGTTTCGGATCACCGAGCCCGAACGTTATA
AAAAAATCGCCACGTTGCTGGAGGAGACTCGCCGCAGCAGGGAACAATATATCGCGTTGGTGGTTGACAGGTTACAAAAC
GAACTGGGTCACGCGGGGATCAAGGCTCAAATTACAGGACGCCCCAAGCACATTTACAGCATTTACAGAAAAATGAAACG
CAAGGGTGTGGATTTCAAGGAAATCCAGGATACCCGGGCCGTTCGCATTCTCGTGGACGAAGTCAAGGATTGCTACGCGG
CATTGGGATTGGTGCACAACCTCTGGATGCCGATTCCCAAGGAGTTCGACGACTATATTGCCAAGCCGAAAGGCAATGAC
TACCGGTCGTTGCATACTGCAGTAATCGGTCCGGAAGACAAAGTGGTCGAAGTACAGATCCGGACCCATGAGATGCACCG
TCATTCAGAGATGGGTGTCGCCGCGCATTGGCGCTACAAGGAAGGTGCAAAGCGGGACGCGCGGTATGAGGAAAAGATCG
CCTGGTTAAGGCAGATACTGGAGTGGAAGAATGATGTCGAGGATGCGGGAGAACTGGCTGAGCATTTCAAGGTGGCACTG
TTCGAAGATTCGGTCTATGTACTGACGCCGCAAGGAAAGGTGATCGCGCTGCCCAAGGGAGCCACTCCCGTGGATTTTGC
ATACCATGTGCATAGCGACCTGGGGCACCGTTGCCGCGGTGCCAAGGTGGATGGCGTCATGGTGCCCCTCGATTACCCGC
TGCAAAATGCGCAAAGAGTGGAGATCATTTCGACCAAGCAGGGGGGACCCAGCCGCGACTGGCTCAACCCTGCATTGGGT
TATATCAGGAGCCAGCGCGCCCGGGCGAAAGTGAGGCAATGGTTCAGCAGTGAAATGCGTACTGTTTCCCTCACCCAAGC
CCGGGCTTTGGTGGAAAAGGAACTGCAGCGCCATGGCATGACTGCGATGAGCCTGGATAAGCTGGCAGTGGAGTTCAAAT
TTGCCAGACTTGATTCCTTTCTTGCAGCCGTTGCAAGGGGTGATATCAATGCGCGCCAGCTATCGGCATTCTTGAGCGGG
GAGACAGAATCGGGTCTACCCGGCCCCGAAACGCCAGTCATCAAAAAGCAGACTCTCACTTCGAAACCGCAGGGAGACAT
ACTCGTCGTCGGGGTCGACAAGTTGCTCACCGTACTGGCAAAGTGCTGCAAGCCTGTACCCCCTGATCCCATCATTGGTT
TTGTCACCCGTGGGCGGGGAATCACCATCCACAGGGCGAATTGCGCCAATGTGGCCCGGTTGTCAGGAGAAGGCAGGGCG
CGCCTCGTCAGTACCCAATGGGATTTATCCAAAAATGCCAGTTACGCCGTAGAGGTGGAGATTCGGGCGCAGGATCGTCA
AGGATTGCTGAGCGATATTTCGGGTGTTCTGTCGCGCGAAAAAATTGATGTAGTCGCTACCCGCGCCCATAGCGGCGACC
TGAATGCGACCATGCAGTTCGTGATCAAGATTGGCAATGTCGATCAATTGCAGCGTGTTCTCGGGCTGATAGCCGGCGTC
CCCGGCGTCATATCGGCCACGCGCAGATAA

Upstream 100 bases:

>100_bases
CAGCAACGCTTTAACCGGAAATAAAGCAATTATGAAAGGACATGAGTAACATGGGGGCTCAATTGAAAAAGGCCGTTCCA
ATACTCACTACACCCGTTCC

Downstream 100 bases:

>100_bases
ACGTAATTCAAGTTCCGGCAATGTCCTGTCAGCCCTCCGATACTCCGCACAGCAATTGATCGCAACCCGACCAATGGCAG
CAGGTGCGTTCGCAACTCCC

Product: (p)ppGpp synthetase SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MSAPSLISGKGDGPFRLHSPGSIFSPTEIDLLREAQVFCEPLYMDKSLPNGELLSSHVDGLAAILAGLRVDSDTLAAGVL
HAVPEYLEGYEEELATAFGPIVAHLVEGVRRVGRIQVLRSANSTDNIGNGSAQIEALRKMLLAMAEDIRVVIIALADRLQ
TMRYVAARNVAGRWEAAEVTLNIFAPLANRLGLWQVKWELEDLSFRITEPERYKKIATLLEETRRSREQYIALVVDRLQN
ELGHAGIKAQITGRPKHIYSIYRKMKRKGVDFKEIQDTRAVRILVDEVKDCYAALGLVHNLWMPIPKEFDDYIAKPKGND
YRSLHTAVIGPEDKVVEVQIRTHEMHRHSEMGVAAHWRYKEGAKRDARYEEKIAWLRQILEWKNDVEDAGELAEHFKVAL
FEDSVYVLTPQGKVIALPKGATPVDFAYHVHSDLGHRCRGAKVDGVMVPLDYPLQNAQRVEIISTKQGGPSRDWLNPALG
YIRSQRARAKVRQWFSSEMRTVSLTQARALVEKELQRHGMTAMSLDKLAVEFKFARLDSFLAAVARGDINARQLSAFLSG
ETESGLPGPETPVIKKQTLTSKPQGDILVVGVDKLLTVLAKCCKPVPPDPIIGFVTRGRGITIHRANCANVARLSGEGRA
RLVSTQWDLSKNASYAVEVEIRAQDRQGLLSDISGVLSREKIDVVATRAHSGDLNATMQFVIKIGNVDQLQRVLGLIAGV
PGVISATRR

Sequences:

>Translated_729_residues
MSAPSLISGKGDGPFRLHSPGSIFSPTEIDLLREAQVFCEPLYMDKSLPNGELLSSHVDGLAAILAGLRVDSDTLAAGVL
HAVPEYLEGYEEELATAFGPIVAHLVEGVRRVGRIQVLRSANSTDNIGNGSAQIEALRKMLLAMAEDIRVVIIALADRLQ
TMRYVAARNVAGRWEAAEVTLNIFAPLANRLGLWQVKWELEDLSFRITEPERYKKIATLLEETRRSREQYIALVVDRLQN
ELGHAGIKAQITGRPKHIYSIYRKMKRKGVDFKEIQDTRAVRILVDEVKDCYAALGLVHNLWMPIPKEFDDYIAKPKGND
YRSLHTAVIGPEDKVVEVQIRTHEMHRHSEMGVAAHWRYKEGAKRDARYEEKIAWLRQILEWKNDVEDAGELAEHFKVAL
FEDSVYVLTPQGKVIALPKGATPVDFAYHVHSDLGHRCRGAKVDGVMVPLDYPLQNAQRVEIISTKQGGPSRDWLNPALG
YIRSQRARAKVRQWFSSEMRTVSLTQARALVEKELQRHGMTAMSLDKLAVEFKFARLDSFLAAVARGDINARQLSAFLSG
ETESGLPGPETPVIKKQTLTSKPQGDILVVGVDKLLTVLAKCCKPVPPDPIIGFVTRGRGITIHRANCANVARLSGEGRA
RLVSTQWDLSKNASYAVEVEIRAQDRQGLLSDISGVLSREKIDVVATRAHSGDLNATMQFVIKIGNVDQLQRVLGLIAGV
PGVISATRR
>Mature_728_residues
SAPSLISGKGDGPFRLHSPGSIFSPTEIDLLREAQVFCEPLYMDKSLPNGELLSSHVDGLAAILAGLRVDSDTLAAGVLH
AVPEYLEGYEEELATAFGPIVAHLVEGVRRVGRIQVLRSANSTDNIGNGSAQIEALRKMLLAMAEDIRVVIIALADRLQT
MRYVAARNVAGRWEAAEVTLNIFAPLANRLGLWQVKWELEDLSFRITEPERYKKIATLLEETRRSREQYIALVVDRLQNE
LGHAGIKAQITGRPKHIYSIYRKMKRKGVDFKEIQDTRAVRILVDEVKDCYAALGLVHNLWMPIPKEFDDYIAKPKGNDY
RSLHTAVIGPEDKVVEVQIRTHEMHRHSEMGVAAHWRYKEGAKRDARYEEKIAWLRQILEWKNDVEDAGELAEHFKVALF
EDSVYVLTPQGKVIALPKGATPVDFAYHVHSDLGHRCRGAKVDGVMVPLDYPLQNAQRVEIISTKQGGPSRDWLNPALGY
IRSQRARAKVRQWFSSEMRTVSLTQARALVEKELQRHGMTAMSLDKLAVEFKFARLDSFLAAVARGDINARQLSAFLSGE
TESGLPGPETPVIKKQTLTSKPQGDILVVGVDKLLTVLAKCCKPVPPDPIIGFVTRGRGITIHRANCANVARLSGEGRAR
LVSTQWDLSKNASYAVEVEIRAQDRQGLLSDISGVLSREKIDVVATRAHSGDLNATMQFVIKIGNVDQLQRVLGLIAGVP
GVISATRR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=722, Percent_Identity=42.797783933518, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1790082, Length=708, Percent_Identity=34.3220338983051, Blast_Score=420, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 80966; Mature: 80835

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSLISGKGDGPFRLHSPGSIFSPTEIDLLREAQVFCEPLYMDKSLPNGELLSSHVDG
CCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LAAILAGLRVDSDTLAAGVLHAVPEYLEGYEEELATAFGPIVAHLVEGVRRVGRIQVLRS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANSTDNIGNGSAQIEALRKMLLAMAEDIRVVIIALADRLQTMRYVAARNVAGRWEAAEVT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEE
LNIFAPLANRLGLWQVKWELEDLSFRITEPERYKKIATLLEETRRSREQYIALVVDRLQN
HHHHHHHHHCCCEEEEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGHAGIKAQITGRPKHIYSIYRKMKRKGVDFKEIQDTRAVRILVDEVKDCYAALGLVHN
HHCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWMPIPKEFDDYIAKPKGNDYRSLHTAVIGPEDKVVEVQIRTHEMHRHSEMGVAAHWRYK
HCCCCCCHHHHHHCCCCCCCHHHHHHEEECCCCCEEEEEEEHHHHHHHHCCCCEEEEECC
EGAKRDARYEEKIAWLRQILEWKNDVEDAGELAEHFKVALFEDSVYVLTPQGKVIALPKG
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCEEEECCCCCEEEECCC
ATPVDFAYHVHSDLGHRCRGAKVDGVMVPLDYPLQNAQRVEIISTKQGGPSRDWLNPALG
CCCCHHHHHHHHHHCHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
YIRSQRARAKVRQWFSSEMRTVSLTQARALVEKELQRHGMTAMSLDKLAVEFKFARLDSF
HHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LAAVARGDINARQLSAFLSGETESGLPGPETPVIKKQTLTSKPQGDILVVGVDKLLTVLA
HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCEECHHCCCCCCCCEEEEEHHHHHHHHH
KCCKPVPPDPIIGFVTRGRGITIHRANCANVARLSGEGRARLVSTQWDLSKNASYAVEVE
HHCCCCCCCCCEEEEECCCCEEEEECCCCCHHHCCCCCCEEEEEEECCCCCCCCEEEEEE
IRAQDRQGLLSDISGVLSREKIDVVATRAHSGDLNATMQFVIKIGNVDQLQRVLGLIAGV
EECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHEEEEEEEECCHHHHHHHHHHHHCC
PGVISATRR
CHHHHCCCC
>Mature Secondary Structure 
SAPSLISGKGDGPFRLHSPGSIFSPTEIDLLREAQVFCEPLYMDKSLPNGELLSSHVDG
CCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LAAILAGLRVDSDTLAAGVLHAVPEYLEGYEEELATAFGPIVAHLVEGVRRVGRIQVLRS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANSTDNIGNGSAQIEALRKMLLAMAEDIRVVIIALADRLQTMRYVAARNVAGRWEAAEVT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEE
LNIFAPLANRLGLWQVKWELEDLSFRITEPERYKKIATLLEETRRSREQYIALVVDRLQN
HHHHHHHHHCCCEEEEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGHAGIKAQITGRPKHIYSIYRKMKRKGVDFKEIQDTRAVRILVDEVKDCYAALGLVHN
HHCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWMPIPKEFDDYIAKPKGNDYRSLHTAVIGPEDKVVEVQIRTHEMHRHSEMGVAAHWRYK
HCCCCCCHHHHHHCCCCCCCHHHHHHEEECCCCCEEEEEEEHHHHHHHHCCCCEEEEECC
EGAKRDARYEEKIAWLRQILEWKNDVEDAGELAEHFKVALFEDSVYVLTPQGKVIALPKG
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCEEEECCCCCEEEECCC
ATPVDFAYHVHSDLGHRCRGAKVDGVMVPLDYPLQNAQRVEIISTKQGGPSRDWLNPALG
CCCCHHHHHHHHHHCHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
YIRSQRARAKVRQWFSSEMRTVSLTQARALVEKELQRHGMTAMSLDKLAVEFKFARLDSF
HHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LAAVARGDINARQLSAFLSGETESGLPGPETPVIKKQTLTSKPQGDILVVGVDKLLTVLA
HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCEECHHCCCCCCCCEEEEEHHHHHHHHH
KCCKPVPPDPIIGFVTRGRGITIHRANCANVARLSGEGRARLVSTQWDLSKNASYAVEVE
HHCCCCCCCCCEEEEECCCCEEEEECCCCCHHHCCCCCCEEEEEEECCCCCCCCEEEEEE
IRAQDRQGLLSDISGVLSREKIDVVATRAHSGDLNATMQFVIKIGNVDQLQRVLGLIAGV
EECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCHHEEEEEEEECCHHHHHHHHHHHHCC
PGVISATRR
CHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]