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The map label for this gene is pyrB

Identifier: 82703596

GI number: 82703596

Start: 2832276

End: 2833226

Strand: Direct

Name: pyrB

Synonym: Nmul_A2481

Alternate gene names: 82703596

Gene position: 2832276-2833226 (Clockwise)

Preceding gene: 82703595

Following gene: 82703597

Centisome position: 88.95

GC content: 54.99

Gene sequence:

>951_bases
ATGAGTAATAATCCCCAGCTGAACAAAAGCGGTCAACTGCAGCATCTCTTGACTACCGAGGGACTGCCCGCCTCGATTCT
CCTGAATATTCTCGATACTGCCGAACCGTTCGTCGGGGTCACGGAACGTGACGTCAAGAAAGTGCCGCTGTTGCGCGGAA
AATCCATATTCAACCTGTTCTTCGAACCTAGTACGCGGACACGCACGACATTTGAAATTGCCGCAAAACGGTTGTCTGCC
GATGTCATCAATCTCAACATTGCAGCCTCGTCCCAATCCAAAGGAGAGACACTGCTGGACACAGTGAACAATCTTTCCGC
CATGCATGCCGATATGTTCGTGGTGAGGCATGCTCAGAGCGGCGCTGCACATCTCATTGCGCAGCATGTCCGCTCTGACA
TTCACGTCATCAATGCAGGGGATGGCCGGCACGCGCATCCCACGCAGGCCCTGCTCGACATGTTTACCATTCGTCGCTGC
AAGCGCGACTTTCATAACCTGCGCGTCGCCATAGTGGGCGACATCCTTCATTCCCGGGTGGCCCGCTCGCAGATCCATGC
GTTGACGACGCTCGGGGTACCGGAAGTACGTGTCATCGCACCCAAGACATTGCTGCCCGCCAAGGTCGAACGTCTGGGCG
TGCATGTTTACCATGATATGGCGAAAGGACTGAAGGACGTCGATGTGCTCATGATGCTTCGTCTGCAAAACGAACGCATG
CTCAGCGCGCGCCTTCCGAGCACCGAAGAATATTTCAAATATTACGGGCTCACTCCCGAAAAACTGGCGCTGGCGAAGCC
GGATGCAATCGTTCTGCATCCCGGACCCATGAACCGTGGGGTTGAGATCGATTCGGTCGTAGCCGATGGGAAGCAATCTG
TAATTTTGCCCCAGGTAACATTCGGCATCGCAGTGCGCATGGCGGTAATGTCCATTCTGGCCGGAAGCTGA

Upstream 100 bases:

>100_bases
ATTGGTGCAGCGCTGGATGTACCCCCTGCAAAAATGCTCGCGCTGGAAAAGAAAGCAGATGGAAAGCTGGGCCTGAGCCT
GTACGACAAGCGCCCGGGCA

Downstream 100 bases:

>100_bases
AAGTCATGAACATCGCCATCCGCAATGGTCGCGTCATCGACCCGAAAAACAGCTTCGATCGCGTAACGGACATTTACATC
CAGTCAGGCAAAATCGCCTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSNNPQLNKSGQLQHLLTTEGLPASILLNILDTAEPFVGVTERDVKKVPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSA
DVINLNIAASSQSKGETLLDTVNNLSAMHADMFVVRHAQSGAAHLIAQHVRSDIHVINAGDGRHAHPTQALLDMFTIRRC
KRDFHNLRVAIVGDILHSRVARSQIHALTTLGVPEVRVIAPKTLLPAKVERLGVHVYHDMAKGLKDVDVLMMLRLQNERM
LSARLPSTEEYFKYYGLTPEKLALAKPDAIVLHPGPMNRGVEIDSVVADGKQSVILPQVTFGIAVRMAVMSILAGS

Sequences:

>Translated_316_residues
MSNNPQLNKSGQLQHLLTTEGLPASILLNILDTAEPFVGVTERDVKKVPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSA
DVINLNIAASSQSKGETLLDTVNNLSAMHADMFVVRHAQSGAAHLIAQHVRSDIHVINAGDGRHAHPTQALLDMFTIRRC
KRDFHNLRVAIVGDILHSRVARSQIHALTTLGVPEVRVIAPKTLLPAKVERLGVHVYHDMAKGLKDVDVLMMLRLQNERM
LSARLPSTEEYFKYYGLTPEKLALAKPDAIVLHPGPMNRGVEIDSVVADGKQSVILPQVTFGIAVRMAVMSILAGS
>Mature_315_residues
SNNPQLNKSGQLQHLLTTEGLPASILLNILDTAEPFVGVTERDVKKVPLLRGKSIFNLFFEPSTRTRTTFEIAAKRLSAD
VINLNIAASSQSKGETLLDTVNNLSAMHADMFVVRHAQSGAAHLIAQHVRSDIHVINAGDGRHAHPTQALLDMFTIRRCK
RDFHNLRVAIVGDILHSRVARSQIHALTTLGVPEVRVIAPKTLLPAKVERLGVHVYHDMAKGLKDVDVLMMLRLQNERML
SARLPSTEEYFKYYGLTPEKLALAKPDAIVLHPGPMNRGVEIDSVVADGKQSVILPQVTFGIAVRMAVMSILAGS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=34.6153846153846, Blast_Score=158, Evalue=6e-39,
Organism=Escherichia coli, GI2367364, Length=273, Percent_Identity=35.1648351648352, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1786469, Length=202, Percent_Identity=28.7128712871287, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=292, Percent_Identity=33.9041095890411, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322331, Length=276, Percent_Identity=34.7826086956522, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=35.2380952380952, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_NITMU (Q2Y651)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413162.1
- HSSP:   P0A786
- ProteinModelPortal:   Q2Y651
- SMR:   Q2Y651
- STRING:   Q2Y651
- GeneID:   3784830
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2481
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   NMUL323848:NMUL_A2481-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34727; Mature: 34596

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNPQLNKSGQLQHLLTTEGLPASILLNILDTAEPFVGVTERDVKKVPLLRGKSIFNLF
CCCCCCCCCCCCEEEEHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCEEEEE
FEPSTRTRTTFEIAAKRLSADVINLNIAASSQSKGETLLDTVNNLSAMHADMFVVRHAQS
ECCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCC
GAAHLIAQHVRSDIHVINAGDGRHAHPTQALLDMFTIRRCKRDFHNLRVAIVGDILHSRV
CHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
ARSQIHALTTLGVPEVRVIAPKTLLPAKVERLGVHVYHDMAKGLKDVDVLMMLRLQNERM
HHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHH
LSARLPSTEEYFKYYGLTPEKLALAKPDAIVLHPGPMNRGVEIDSVVADGKQSVILPQVT
HHHCCCCHHHHHHHCCCCHHHHEECCCCEEEECCCCCCCCCCHHHHHCCCCCCEEEHHHH
FGIAVRMAVMSILAGS
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNNPQLNKSGQLQHLLTTEGLPASILLNILDTAEPFVGVTERDVKKVPLLRGKSIFNLF
CCCCCCCCCCCEEEEHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCEEEEE
FEPSTRTRTTFEIAAKRLSADVINLNIAASSQSKGETLLDTVNNLSAMHADMFVVRHAQS
ECCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCC
GAAHLIAQHVRSDIHVINAGDGRHAHPTQALLDMFTIRRCKRDFHNLRVAIVGDILHSRV
CHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
ARSQIHALTTLGVPEVRVIAPKTLLPAKVERLGVHVYHDMAKGLKDVDVLMMLRLQNERM
HHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHH
LSARLPSTEEYFKYYGLTPEKLALAKPDAIVLHPGPMNRGVEIDSVVADGKQSVILPQVT
HHHCCCCHHHHHHHCCCCHHHHEECCCCEEEECCCCCCCCCCHHHHHCCCCCCEEEHHHH
FGIAVRMAVMSILAGS
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA