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The map label for this gene is pyrC' [H]

Identifier: 82703597

GI number: 82703597

Start: 2833232

End: 2834506

Strand: Direct

Name: pyrC' [H]

Synonym: Nmul_A2482

Alternate gene names: 82703597

Gene position: 2833232-2834506 (Clockwise)

Preceding gene: 82703596

Following gene: 82703598

Centisome position: 88.98

GC content: 59.29

Gene sequence:

>1275_bases
ATGAACATCGCCATCCGCAATGGTCGCGTCATCGACCCGAAAAACAGCTTCGATCGCGTAACGGACATTTACATCCAGTC
AGGCAAAATCGCCTCTCTCGGAGCCGTCCCGCCAGGCTTTGAGGCACGCCGGGAAATAAACGCGCAGGGATTGATCGTGT
GTCCCGGGCTTGTGGATCTGTCGGCCCGGCTACGGGAACCCGGCCTGGAATACAAGGCGACGCTTGAATCGGAAATGGGA
GCCGCGGTGGCGGGGGGTGTTACAAGCCTGGCATGTCCCCCCGATACCGATCCTGTGCTGGACGAACCCGGGCTGGTGGA
AATGCTGAAGTATCGCGCAAGGAGCCGCAATCAGACGCGTGTGTATCCCATTGGAGCGCTTACCCGTGGATTGAGAGGGG
AATGGCTGACGGAGATGGCTGAGCTGCACAGCGCGGGATGCGTTGCATTCGGCCAGTCGGACAGGCCGCTTCCCAACAAC
CGGGTGCTCATGCAGGCAATGCAGTATGCCTCCACCTTCGGGTTTTGCCTGTGGCTGCGTCCCCAGGATGTAAATCTCGC
TGACGGCGGAGTTGCCCACGATGGGGAAGTGGCAACGCGCCTTGGGTTGGCCCCCATTCCCGTGTGCGCCGAAACTGTCG
CCCTGTCCCACATCATCCTGATGGCAAAAGAAACGGGCGCCCGGGTGCACTTGTGCCGTATCTCCAGCGCGGAAGGTGTG
ACCATGACACGCGCCGCCCGCAAGCAGGGATTATCTATTACCTGCGACGTTGCGGCCAATCACGTCCACCTGTCTGAAAT
GGATATCGGTTTCTTCGATTCCAATTGTCATCTGGTGCCACCGTTGAGAAGTCTGGGAGACCGCGACGCCTTGCGCGCAG
GACTGCTGGATGGCACTATAGATGCCATATGCTCCGACCACGCTCCCGTGGACGAGGATGCAAAGCTGCTGCCCTTTGCC
GAGGCCGAGGCCGGCGCAACCGGCCTCGAATTACTCCTGCCGCTTACACTGAAATGGGCGGCGGAAACGAAGCTGCCCCT
GGTAGTCGCACTATCGAAGATCACGAGGGAACCTGCCCGCATCCTTGGGGTGGAAGCCGGCCATCTCACGCCCGGGGGCA
ATGCCGATCTGTGCATTTTCGACCCTGATCATTACTGGACGATTGAAGCGCCGACACTCAAAAGCCAGGGCAAGAACACG
CCATTCCTGGGCTGGGAACTGCAAGGCAAGGTAAAATATACGCTGATCAACGGAAACGTTGTTTACGTGGACTAG

Upstream 100 bases:

>100_bases
TCGTAGCCGATGGGAAGCAATCTGTAATTTTGCCCCAGGTAACATTCGGCATCGCAGTGCGCATGGCGGTAATGTCCATT
CTGGCCGGAAGCTGAAAGTC

Downstream 100 bases:

>100_bases
GGTCGCAACACGGCATGCATTTTCGAGTTCTCTTCTTTCAAACTGACTGAATTTCCATCAATGAACCCTTTGCTCGATTT
TTCCGGATTGCCGCGCTTTG

Product: dihydroorotase

Products: N-carbamoyl-L-aspartate

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNIAIRNGRVIDPKNSFDRVTDIYIQSGKIASLGAVPPGFEARREINAQGLIVCPGLVDLSARLREPGLEYKATLESEMG
AAVAGGVTSLACPPDTDPVLDEPGLVEMLKYRARSRNQTRVYPIGALTRGLRGEWLTEMAELHSAGCVAFGQSDRPLPNN
RVLMQAMQYASTFGFCLWLRPQDVNLADGGVAHDGEVATRLGLAPIPVCAETVALSHIILMAKETGARVHLCRISSAEGV
TMTRAARKQGLSITCDVAANHVHLSEMDIGFFDSNCHLVPPLRSLGDRDALRAGLLDGTIDAICSDHAPVDEDAKLLPFA
EAEAGATGLELLLPLTLKWAAETKLPLVVALSKITREPARILGVEAGHLTPGGNADLCIFDPDHYWTIEAPTLKSQGKNT
PFLGWELQGKVKYTLINGNVVYVD

Sequences:

>Translated_424_residues
MNIAIRNGRVIDPKNSFDRVTDIYIQSGKIASLGAVPPGFEARREINAQGLIVCPGLVDLSARLREPGLEYKATLESEMG
AAVAGGVTSLACPPDTDPVLDEPGLVEMLKYRARSRNQTRVYPIGALTRGLRGEWLTEMAELHSAGCVAFGQSDRPLPNN
RVLMQAMQYASTFGFCLWLRPQDVNLADGGVAHDGEVATRLGLAPIPVCAETVALSHIILMAKETGARVHLCRISSAEGV
TMTRAARKQGLSITCDVAANHVHLSEMDIGFFDSNCHLVPPLRSLGDRDALRAGLLDGTIDAICSDHAPVDEDAKLLPFA
EAEAGATGLELLLPLTLKWAAETKLPLVVALSKITREPARILGVEAGHLTPGGNADLCIFDPDHYWTIEAPTLKSQGKNT
PFLGWELQGKVKYTLINGNVVYVD
>Mature_424_residues
MNIAIRNGRVIDPKNSFDRVTDIYIQSGKIASLGAVPPGFEARREINAQGLIVCPGLVDLSARLREPGLEYKATLESEMG
AAVAGGVTSLACPPDTDPVLDEPGLVEMLKYRARSRNQTRVYPIGALTRGLRGEWLTEMAELHSAGCVAFGQSDRPLPNN
RVLMQAMQYASTFGFCLWLRPQDVNLADGGVAHDGEVATRLGLAPIPVCAETVALSHIILMAKETGARVHLCRISSAEGV
TMTRAARKQGLSITCDVAANHVHLSEMDIGFFDSNCHLVPPLRSLGDRDALRAGLLDGTIDAICSDHAPVDEDAKLLPFA
EAEAGATGLELLLPLTLKWAAETKLPLVVALSKITREPARILGVEAGHLTPGGNADLCIFDPDHYWTIEAPTLKSQGKNT
PFLGWELQGKVKYTLINGNVVYVD

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=410, Percent_Identity=28.0487804878049, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1786722, Length=441, Percent_Identity=25.1700680272109, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082175, Length=453, Percent_Identity=22.9580573951435, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=394, Percent_Identity=25.8883248730964, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322218, Length=413, Percent_Identity=23.4866828087167, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24642586, Length=412, Percent_Identity=25.9708737864078, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18859883, Length=208, Percent_Identity=28.3653846153846, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.3

Molecular weight: Translated: 45586; Mature: 45586

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAIRNGRVIDPKNSFDRVTDIYIQSGKIASLGAVPPGFEARREINAQGLIVCPGLVDL
CEEEECCCEEECCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHCCCCEEEECCCHHHH
SARLREPGLEYKATLESEMGAAVAGGVTSLACPPDTDPVLDEPGLVEMLKYRARSRNQTR
HHHHCCCCCCEEEEHHHHCCCCHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
VYPIGALTRGLRGEWLTEMAELHSAGCVAFGQSDRPLPNNRVLMQAMQYASTFGFCLWLR
EEEHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCEEEEEEC
PQDVNLADGGVAHDGEVATRLGLAPIPVCAETVALSHIILMAKETGARVHLCRISSAEGV
CCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCE
TMTRAARKQGLSITCDVAANHVHLSEMDIGFFDSNCHLVPPLRSLGDRDALRAGLLDGTI
EEEHHHHHCCCEEEEEECCCEEEEEECCCEEECCCCEECCCHHHCCCHHHHHHHCCCCCH
DAICSDHAPVDEDAKLLPFAEAEAGATGLELLLPLTLKWAAETKLPLVVALSKITREPAR
HHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEECCCCCCHHEEEHHHHHCCCHH
ILGVEAGHLTPGGNADLCIFDPDHYWTIEAPTLKSQGKNTPFLGWELQGKVKYTLINGNV
HEEEECCCCCCCCCCCEEEECCCCEEEEECCCHHCCCCCCCEEEEEECCEEEEEEECCEE
VYVD
EEEC
>Mature Secondary Structure
MNIAIRNGRVIDPKNSFDRVTDIYIQSGKIASLGAVPPGFEARREINAQGLIVCPGLVDL
CEEEECCCEEECCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHCCCCEEEECCCHHHH
SARLREPGLEYKATLESEMGAAVAGGVTSLACPPDTDPVLDEPGLVEMLKYRARSRNQTR
HHHHCCCCCCEEEEHHHHCCCCHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
VYPIGALTRGLRGEWLTEMAELHSAGCVAFGQSDRPLPNNRVLMQAMQYASTFGFCLWLR
EEEHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHCEEEEEEC
PQDVNLADGGVAHDGEVATRLGLAPIPVCAETVALSHIILMAKETGARVHLCRISSAEGV
CCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCE
TMTRAARKQGLSITCDVAANHVHLSEMDIGFFDSNCHLVPPLRSLGDRDALRAGLLDGTI
EEEHHHHHCCCEEEEEECCCEEEEEECCCEEECCCCEECCCHHHCCCHHHHHHHCCCCCH
DAICSDHAPVDEDAKLLPFAEAEAGATGLELLLPLTLKWAAETKLPLVVALSKITREPAR
HHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEECCCCCCHHEEEHHHHHCCCHH
ILGVEAGHLTPGGNADLCIFDPDHYWTIEAPTLKSQGKNTPFLGWELQGKVKYTLINGNV
HEEEECCCCCCCCCCCEEEECCCCEEEEECCCHHCCCCCCCEEEEEECCEEEEEEECCEE
VYVD
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; H2O

Specific reaction: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]