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The map label for this gene is queA

Identifier: 82703545

GI number: 82703545

Start: 2768362

End: 2769432

Strand: Reverse

Name: queA

Synonym: Nmul_A2430

Alternate gene names: 82703545

Gene position: 2769432-2768362 (Counterclockwise)

Preceding gene: 82703546

Following gene: 82703544

Centisome position: 86.97

GC content: 57.24

Gene sequence:

>1071_bases
ATGAAAATTCAGGATTTCGACTTCGACCTTCCTCCTGAACTCATTGCGCAGTTTCCGGCCGAGAAGCGGGGCAATAGCCG
CATGCTGCATCTCGACGCCACAAGCGGCGCACTGCAGGACAGGATGTTTGCCGACCTTCCATCGTATTTGCGTCCCGGCG
ACGTGATTGTCTTTAACGATACCCGCGTCATCAAAGCGCGCCTGTATGGCGTGAGAGATACCGGCGGCAGGGTCGAGGTC
CTGGTGGAGCGTGTGCTGGACAGCCATTGCGCGCTGGCTGCTATTCGCGCCAGTCATTCACCGAAACCGGGATCGAAGCT
TTTCCTTGCTGGTGCGATAGAGGTCACCGTCCTGTCCCGTGAGCACGGTTTTTATATCCTGCAATTCGAGCACGAGAGTG
GGGTCATGGAGTTGCTGGAACGTTACGGCAATCTGCCTTTGCCTCCCTATATATCGCGCCCCGTGGAAAAATCCGACGAG
CTGCGGTATCAGACCGTTTATGCGGCTCGACCGGGTGCAGTGGCGGCTCCAACGGCGGGACTGCATTTCGATGAGCACAT
GCTGGGGTTGTTGCGCAAAATGGGTGTGGTGATGGCGCATGTGACGCTGCATGTGGGCTCCGGCACTTTCCAGCCGGTGC
GGGTGGAAAATATTGCCGATCACAGGATGCACAGCGAAGTTTTTTATGTCTCTGCGGAAACCGTGGAATGCATTAGGCAT
GCGAAAGCCGAGGGCGGCCGGGTACTGGCAGTGGGCACCACCTCGCTCAGGGCACTGGAGGCCGCGGCAGCATTCGCGGG
CGAGCGGGCGGAAGCGGATAATGGGGATGGCGTTCGGATAAAATGCGGAGCCGGCGAGACCGATATTTTCATTACGCCGG
GTTATCAGTTCCGGGTAGTGGATTGCCTGCTGACCAACTTTCATTTGCCCCGCTCGACCTTGTTAATGCTCGTGTCCGCA
TTCGGTGGCGTCGAAAATATCCGGTGTGCGTATCGGCATGCGATCAAAGAACGCTATCGCTTTTTCAGTTTTGGCGATGC
GATGCTGATCGGAAGGGCAGAGGGGATATAG

Upstream 100 bases:

>100_bases
CGGCAAAGCTTTTCCTGTTCTTTACAGGAGGGGCATTATCACTGAAAACCCCATTTGCGGCAACCCGTAGCGCGATTCCT
GCTGCCGTATAATACGTCCA

Downstream 100 bases:

>100_bases
ATTAAAGCCTGGAATAACACTCCGCGGACAGAATCGTTTGCCTTCAGTCATAGCGCTATCTTCTAAAAAACTGCTTGGTC
TTCTTTCTATAAAACTGCCA

Product: S-adenosylmethionine

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKIQDFDFDLPPELIAQFPAEKRGNSRMLHLDATSGALQDRMFADLPSYLRPGDVIVFNDTRVIKARLYGVRDTGGRVEV
LVERVLDSHCALAAIRASHSPKPGSKLFLAGAIEVTVLSREHGFYILQFEHESGVMELLERYGNLPLPPYISRPVEKSDE
LRYQTVYAARPGAVAAPTAGLHFDEHMLGLLRKMGVVMAHVTLHVGSGTFQPVRVENIADHRMHSEVFYVSAETVECIRH
AKAEGGRVLAVGTTSLRALEAAAAFAGERAEADNGDGVRIKCGAGETDIFITPGYQFRVVDCLLTNFHLPRSTLLMLVSA
FGGVENIRCAYRHAIKERYRFFSFGDAMLIGRAEGI

Sequences:

>Translated_356_residues
MKIQDFDFDLPPELIAQFPAEKRGNSRMLHLDATSGALQDRMFADLPSYLRPGDVIVFNDTRVIKARLYGVRDTGGRVEV
LVERVLDSHCALAAIRASHSPKPGSKLFLAGAIEVTVLSREHGFYILQFEHESGVMELLERYGNLPLPPYISRPVEKSDE
LRYQTVYAARPGAVAAPTAGLHFDEHMLGLLRKMGVVMAHVTLHVGSGTFQPVRVENIADHRMHSEVFYVSAETVECIRH
AKAEGGRVLAVGTTSLRALEAAAAFAGERAEADNGDGVRIKCGAGETDIFITPGYQFRVVDCLLTNFHLPRSTLLMLVSA
FGGVENIRCAYRHAIKERYRFFSFGDAMLIGRAEGI
>Mature_356_residues
MKIQDFDFDLPPELIAQFPAEKRGNSRMLHLDATSGALQDRMFADLPSYLRPGDVIVFNDTRVIKARLYGVRDTGGRVEV
LVERVLDSHCALAAIRASHSPKPGSKLFLAGAIEVTVLSREHGFYILQFEHESGVMELLERYGNLPLPPYISRPVEKSDE
LRYQTVYAARPGAVAAPTAGLHFDEHMLGLLRKMGVVMAHVTLHVGSGTFQPVRVENIADHRMHSEVFYVSAETVECIRH
AKAEGGRVLAVGTTSLRALEAAAAFAGERAEADNGDGVRIKCGAGETDIFITPGYQFRVVDCLLTNFHLPRSTLLMLVSA
FGGVENIRCAYRHAIKERYRFFSFGDAMLIGRAEGI

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=352, Percent_Identity=53.125, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_NITMU (Q2Y6A2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413111.1
- ProteinModelPortal:   Q2Y6A2
- SMR:   Q2Y6A2
- STRING:   Q2Y6A2
- GeneID:   3784124
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2430
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q2Y6A2
- BioCyc:   NMUL323848:NMUL_A2430-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39286; Mature: 39286

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQDFDFDLPPELIAQFPAEKRGNSRMLHLDATSGALQDRMFADLPSYLRPGDVIVFND
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TRVIKARLYGVRDTGGRVEVLVERVLDSHCALAAIRASHSPKPGSKLFLAGAIEVTVLSR
CEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEE
EHGFYILQFEHESGVMELLERYGNLPLPPYISRPVEKSDELRYQTVYAARPGAVAAPTAG
CCCEEEEEEECCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCC
LHFDEHMLGLLRKMGVVMAHVTLHVGSGTFQPVRVENIADHRMHSEVFYVSAETVECIRH
CCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCEEECCHHHHHHCCEEEEEEHHHHHHHHH
AKAEGGRVLAVGTTSLRALEAAAAFAGERAEADNGDGVRIKCGAGETDIFITPGYQFRVV
HHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEH
DCLLTNFHLPRSTLLMLVSAFGGVENIRCAYRHAIKERYRFFSFGDAMLIGRAEGI
HHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCC
>Mature Secondary Structure
MKIQDFDFDLPPELIAQFPAEKRGNSRMLHLDATSGALQDRMFADLPSYLRPGDVIVFND
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TRVIKARLYGVRDTGGRVEVLVERVLDSHCALAAIRASHSPKPGSKLFLAGAIEVTVLSR
CEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEE
EHGFYILQFEHESGVMELLERYGNLPLPPYISRPVEKSDELRYQTVYAARPGAVAAPTAG
CCCEEEEEEECCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCC
LHFDEHMLGLLRKMGVVMAHVTLHVGSGTFQPVRVENIADHRMHSEVFYVSAETVECIRH
CCHHHHHHHHHHHHHHHEEEEEEEECCCCCCCEEECCHHHHHHCCEEEEEEHHHHHHHHH
AKAEGGRVLAVGTTSLRALEAAAAFAGERAEADNGDGVRIKCGAGETDIFITPGYQFRVV
HHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEH
DCLLTNFHLPRSTLLMLVSAFGGVENIRCAYRHAIKERYRFFSFGDAMLIGRAEGI
HHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA