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The map label for this gene is tgt

Identifier: 82703544

GI number: 82703544

Start: 2767140

End: 2768243

Strand: Reverse

Name: tgt

Synonym: Nmul_A2429

Alternate gene names: 82703544

Gene position: 2768243-2767140 (Counterclockwise)

Preceding gene: 82703545

Following gene: 82703543

Centisome position: 86.94

GC content: 55.98

Gene sequence:

>1104_bases
ATGAAATTTCAATTGCATCGAACTGATGGCCTGGCCCGTCGCGGTACCTTGTCGCTCACGCACGGCGTTGTGGAAACGCC
CGCTTTCATGCCGGTGGGTACCTATGGCGCGGTGAAAACAATGTCACCGGCAGATTTGCAAGTCATCAACGCACATATCG
TGCTTGGCAACACCTTCCACTTGTGGCTGCGCCCCGGTCTCCAGGTCATTGAAGCGCATGGTGGATTGCACCGGTTCATG
GGGTGGGACGGGCCCATACTGACCGATTCCGGTGGGTTCCAGGTATTCAGCCTCGGCGTTTTGAGAAAAATCACGGAGCA
GGGGGTGAAGTTCAAGTCTCCCGTCAACGGTGACACGTGCTTTCTTACGCCCGAGGAATCAATGCGCATCCAGTATGTGC
TCAATTCTGACATCGTCATGATATTCGATGAGTGTACGCCTTATCCTGCGGATGAGCGGGCGGTGGGGGAATCCATGGAG
TTGAGCCTGCGCTGGGCGGAGCGGTCAAGGCAGGCGCATGATGAAGGTGGAAACCGCAATGGGCTGTTCGGTATCGTGCA
GGGAGGGATGTACGAAAAACTGCGCGATGAATCCCTGGCAGGGTTGCTGGACATCGGTTTCGATGGCTATGCAATCGGGG
GACTGTCAGTGGGAGAGCCGAAGGCCGACATGCGGCGTATCCTGAGACATACCGCTCCAGGGCTGCCAGCAGGGAAGCCC
CGATACCTCATGGGGGTAGGTACTCCAGCTGATATCGTGGATGCGGTGGCGCAGGGTATGGACATGTTCGATTGCGTGCT
GCCGACGCGCAATGCGCGCAATGGATGGCTGTATACGCGAACCGGAGTCATCAAGCTGCGCAACAGTCAGTATCGCCTCG
ATACCCGTCCGGCGGATGAACAATGTACCTGCTATACTTGCCGGCATTTCAGCCGCGCTTATCTGCATCATTTGCAGCGA
ACCGGCGAAATTCTGGGTGCGCGCCTCAATACACTGCATAACCTGTATTATTATCAGCAGTTGATGGGCGAAATCCGCAC
TGCAATCGAAAACGGGCAGTTCCAGGAATATGCGCAAGCATTTCAAGCACAAACGGCATCGTGA

Upstream 100 bases:

>100_bases
ACTCCGCGGACAGAATCGTTTGCCTTCAGTCATAGCGCTATCTTCTAAAAAACTGCTTGGTCTTCTTTCTATAAAACTGC
CATATTCAGTAGCTTGACCC

Downstream 100 bases:

>100_bases
TAAAATCACGTTCTTTTTATCCCATTACCGGAGAATGTCATGTTGATTAGTGCAGCCTACGCTCAGGCCACCGCACCTGC
CGCACCGCCCGGAGCCGATT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKFQLHRTDGLARRGTLSLTHGVVETPAFMPVGTYGAVKTMSPADLQVINAHIVLGNTFHLWLRPGLQVIEAHGGLHRFM
GWDGPILTDSGGFQVFSLGVLRKITEQGVKFKSPVNGDTCFLTPEESMRIQYVLNSDIVMIFDECTPYPADERAVGESME
LSLRWAERSRQAHDEGGNRNGLFGIVQGGMYEKLRDESLAGLLDIGFDGYAIGGLSVGEPKADMRRILRHTAPGLPAGKP
RYLMGVGTPADIVDAVAQGMDMFDCVLPTRNARNGWLYTRTGVIKLRNSQYRLDTRPADEQCTCYTCRHFSRAYLHHLQR
TGEILGARLNTLHNLYYYQQLMGEIRTAIENGQFQEYAQAFQAQTAS

Sequences:

>Translated_367_residues
MKFQLHRTDGLARRGTLSLTHGVVETPAFMPVGTYGAVKTMSPADLQVINAHIVLGNTFHLWLRPGLQVIEAHGGLHRFM
GWDGPILTDSGGFQVFSLGVLRKITEQGVKFKSPVNGDTCFLTPEESMRIQYVLNSDIVMIFDECTPYPADERAVGESME
LSLRWAERSRQAHDEGGNRNGLFGIVQGGMYEKLRDESLAGLLDIGFDGYAIGGLSVGEPKADMRRILRHTAPGLPAGKP
RYLMGVGTPADIVDAVAQGMDMFDCVLPTRNARNGWLYTRTGVIKLRNSQYRLDTRPADEQCTCYTCRHFSRAYLHHLQR
TGEILGARLNTLHNLYYYQQLMGEIRTAIENGQFQEYAQAFQAQTAS
>Mature_367_residues
MKFQLHRTDGLARRGTLSLTHGVVETPAFMPVGTYGAVKTMSPADLQVINAHIVLGNTFHLWLRPGLQVIEAHGGLHRFM
GWDGPILTDSGGFQVFSLGVLRKITEQGVKFKSPVNGDTCFLTPEESMRIQYVLNSDIVMIFDECTPYPADERAVGESME
LSLRWAERSRQAHDEGGNRNGLFGIVQGGMYEKLRDESLAGLLDIGFDGYAIGGLSVGEPKADMRRILRHTAPGLPAGKP
RYLMGVGTPADIVDAVAQGMDMFDCVLPTRNARNGWLYTRTGVIKLRNSQYRLDTRPADEQCTCYTCRHFSRAYLHHLQR
TGEILGARLNTLHNLYYYQQLMGEIRTAIENGQFQEYAQAFQAQTAS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=44.2857142857143, Blast_Score=295, Evalue=6e-80,
Organism=Escherichia coli, GI1786607, Length=361, Percent_Identity=65.9279778393352, Blast_Score=516, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=40.8839779005525, Blast_Score=281, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=233, Percent_Identity=25.7510729613734, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920510, Length=353, Percent_Identity=45.042492917847, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21355311, Length=168, Percent_Identity=27.9761904761905, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_NITMU (Q2Y6A3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413110.1
- HSSP:   P28720
- ProteinModelPortal:   Q2Y6A3
- SMR:   Q2Y6A3
- STRING:   Q2Y6A3
- GeneID:   3785521
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2429
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q2Y6A3
- ProtClustDB:   PRK00112
- BioCyc:   NMUL323848:NMUL_A2429-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 40876; Mature: 40876

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01109 RIBOSOMAL_L10

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQLHRTDGLARRGTLSLTHGVVETPAFMPVGTYGAVKTMSPADLQVINAHIVLGNTFH
CCEEEECCCCCCCCCCEEECCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCEEE
LWLRPGLQVIEAHGGLHRFMGWDGPILTDSGGFQVFSLGVLRKITEQGVKFKSPVNGDTC
EEECCCHHEEHHCCCHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHCCCEECCCCCCCEE
FLTPEESMRIQYVLNSDIVMIFDECTPYPADERAVGESMELSLRWAERSRQAHDEGGNRN
EECCCCCCEEEEEECCCEEEEEECCCCCCCCHHHCCCCCEEEEEEHHHHHHHHHCCCCCC
GLFGIVQGGMYEKLRDESLAGLLDIGFDGYAIGGLSVGEPKADMRRILRHTAPGLPAGKP
CEEEEECCCHHHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCC
RYLMGVGTPADIVDAVAQGMDMFDCVLPTRNARNGWLYTRTGVIKLRNSQYRLDTRPADE
CEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEEEEEECCEEEECCCCCCC
QCTCYTCRHFSRAYLHHLQRTGEILGARLNTLHNLYYYQQLMGEIRTAIENGQFQEYAQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FQAQTAS
HHHCCCC
>Mature Secondary Structure
MKFQLHRTDGLARRGTLSLTHGVVETPAFMPVGTYGAVKTMSPADLQVINAHIVLGNTFH
CCEEEECCCCCCCCCCEEECCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCEEE
LWLRPGLQVIEAHGGLHRFMGWDGPILTDSGGFQVFSLGVLRKITEQGVKFKSPVNGDTC
EEECCCHHEEHHCCCHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHCCCEECCCCCCCEE
FLTPEESMRIQYVLNSDIVMIFDECTPYPADERAVGESMELSLRWAERSRQAHDEGGNRN
EECCCCCCEEEEEECCCEEEEEECCCCCCCCHHHCCCCCEEEEEEHHHHHHHHHCCCCCC
GLFGIVQGGMYEKLRDESLAGLLDIGFDGYAIGGLSVGEPKADMRRILRHTAPGLPAGKP
CEEEEECCCHHHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCC
RYLMGVGTPADIVDAVAQGMDMFDCVLPTRNARNGWLYTRTGVIKLRNSQYRLDTRPADE
CEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEEEEEEEECCEEEECCCCCCC
QCTCYTCRHFSRAYLHHLQRTGEILGARLNTLHNLYYYQQLMGEIRTAIENGQFQEYAQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FQAQTAS
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA