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The map label for this gene is gph [C]

Identifier: 82703485

GI number: 82703485

Start: 2696634

End: 2697317

Strand: Reverse

Name: gph [C]

Synonym: Nmul_A2370

Alternate gene names: 82703485

Gene position: 2697317-2696634 (Counterclockwise)

Preceding gene: 82703486

Following gene: 82703484

Centisome position: 84.71

GC content: 55.41

Gene sequence:

>684_bases
ATGCATTTTCCCCTGCCGATCAAAGCAGTCATGATCGATCTCGACGGCACCTTGCTGGATACCGCGCCTGATCTTGCCAC
CGCTGCCAACATGATGCTGAAGGAACTGGGAAAAGCCGAGCTTCCGCTGGAAACAATCCAATCCTACATCGGCAAAGGGA
TAGAAAAGCTGGTGAAACGTTCGCTCACGGGCGACCTCGATGGCGAGCCCGACTCAGACCTATTGCGGCGCGCAATGCCG
CTGTATGAGCGCAGCTACGAAAAAACATTGTATGTCGATACGCGCGCTTACCCTGGCGTGCGCGAGGGATTGAACGCTTT
GCGTGCAGGCGGCTTCCGGCTCGCCTGCGTGACGAACAAGGCAGAGGCTTTTACCTTGCCGCTGCTTCGTGCGGCGGAAC
TGCTGGACTACTTCGATATCGTGGTTTCCGGGGATAGCTTGCCGAAAAAGAAGCCGGATCCGATGCCTTTGCTGCACGCT
TGTGAACGTTTCGAAATCCAGCCGCATGACATGCTGCTGGTCGGGGATTCGCTAAATGATGCCCAGGCTGCGCGCGCGGC
GGGTTCTCACGTATTTTGTGTTCCCTATGGATACAATGAAGGACGCGACGTGTACGAACTTGATTGCGACGCGATCGTCC
CATCGTTATATGAGGCAACAAAGCTGATCCAGAAATCCTCATGA

Upstream 100 bases:

>100_bases
TCGTCGCCTATACGAACAAGCCTTATTATTTTTTAATGAATATGGATAATACGACGTGAACAAAGGCCTCTCTCCGCTTG
CACGTACTTCTGCTCCTGAT

Downstream 100 bases:

>100_bases
GTAACGGAAAAACGGAAAAGGAAAGATATGCAACCCAGGGATGGTGCTACTGGCACTGCTGGGGTTGGCGTTGGCGGCTC
GATAAATATTGATCCGTTCT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MHFPLPIKAVMIDLDGTLLDTAPDLATAANMMLKELGKAELPLETIQSYIGKGIEKLVKRSLTGDLDGEPDSDLLRRAMP
LYERSYEKTLYVDTRAYPGVREGLNALRAGGFRLACVTNKAEAFTLPLLRAAELLDYFDIVVSGDSLPKKKPDPMPLLHA
CERFEIQPHDMLLVGDSLNDAQAARAAGSHVFCVPYGYNEGRDVYELDCDAIVPSLYEATKLIQKSS

Sequences:

>Translated_227_residues
MHFPLPIKAVMIDLDGTLLDTAPDLATAANMMLKELGKAELPLETIQSYIGKGIEKLVKRSLTGDLDGEPDSDLLRRAMP
LYERSYEKTLYVDTRAYPGVREGLNALRAGGFRLACVTNKAEAFTLPLLRAAELLDYFDIVVSGDSLPKKKPDPMPLLHA
CERFEIQPHDMLLVGDSLNDAQAARAAGSHVFCVPYGYNEGRDVYELDCDAIVPSLYEATKLIQKSS
>Mature_227_residues
MHFPLPIKAVMIDLDGTLLDTAPDLATAANMMLKELGKAELPLETIQSYIGKGIEKLVKRSLTGDLDGEPDSDLLRRAMP
LYERSYEKTLYVDTRAYPGVREGLNALRAGGFRLACVTNKAEAFTLPLLRAAELLDYFDIVVSGDSLPKKKPDPMPLLHA
CERFEIQPHDMLLVGDSLNDAQAARAAGSHVFCVPYGYNEGRDVYELDCDAIVPSLYEATKLIQKSS

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Escherichia coli, GI1789787, Length=232, Percent_Identity=39.2241379310345, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPH_NITMU (Q2Y6G2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413051.1
- ProteinModelPortal:   Q2Y6G2
- SMR:   Q2Y6G2
- STRING:   Q2Y6G2
- GeneID:   3785307
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2370
- eggNOG:   COG0546
- HOGENOM:   HBG742904
- OMA:   DSSNDAQ
- PhylomeDB:   Q2Y6G2
- ProtClustDB:   PRK13222
- BioCyc:   NMUL323848:NMUL_A2370-MONOMER
- HAMAP:   MF_00495
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR01449

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.18

Molecular weight: Translated: 25018; Mature: 25018

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: ACT_SITE 13-13

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFPLPIKAVMIDLDGTLLDTAPDLATAANMMLKELGKAELPLETIQSYIGKGIEKLVKR
CCCCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SLTGDLDGEPDSDLLRRAMPLYERSYEKTLYVDTRAYPGVREGLNALRAGGFRLACVTNK
HHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECC
AEAFTLPLLRAAELLDYFDIVVSGDSLPKKKPDPMPLLHACERFEIQPHDMLLVGDSLND
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCH
AQAARAAGSHVFCVPYGYNEGRDVYELDCDAIVPSLYEATKLIQKSS
HHHHHHCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHFPLPIKAVMIDLDGTLLDTAPDLATAANMMLKELGKAELPLETIQSYIGKGIEKLVKR
CCCCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SLTGDLDGEPDSDLLRRAMPLYERSYEKTLYVDTRAYPGVREGLNALRAGGFRLACVTNK
HHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEECC
AEAFTLPLLRAAELLDYFDIVVSGDSLPKKKPDPMPLLHACERFEIQPHDMLLVGDSLND
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCH
AQAARAAGSHVFCVPYGYNEGRDVYELDCDAIVPSLYEATKLIQKSS
HHHHHHCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA