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The map label for this gene is trpE [H]

Identifier: 82703484

GI number: 82703484

Start: 2695012

End: 2696493

Strand: Reverse

Name: trpE [H]

Synonym: Nmul_A2369

Alternate gene names: 82703484

Gene position: 2696493-2695012 (Counterclockwise)

Preceding gene: 82703485

Following gene: 82703480

Centisome position: 84.68

GC content: 57.96

Gene sequence:

>1482_bases
ATGACGGAAGCAGAATTCCATGCTCTGGCGACAAAGGGCTACACCCATATTCCTGTCGTCCTGGAAACCTTTGCCGATCT
TGACACCCCGTTGTCGGTTTATCTGAAGCTGGCAAATGAGCCTTATTCCTATCTGCTGGAATCCGTTCAGGGAGGCGAGC
GTTTTGGACGTTATTCAGTTATCGGCTTACCCGCCCGCAACCGAATCGAAGTGCGCGGCAACGATGTCAGCGTAATCGAC
GGCAGCACCAGCCAGGTATTCCAGTCGGAAGATCCTCTTGCTTTCGTTCAATCCTATCTGGCGCGCTTCAAGGCGGCACC
TTATCCCGGCTTGCCGCGGTTTTGCGGCGGTCTCGCGGGCTACTTCGCGTACGATACGGTACGTTATATCGAGCGCAGGC
TCGCGGGTGGGTGCGCGTACAGGCTGCCTGACACACTGGATACGCCCGACATCCTGCTGCTGGTTTCGGAAGAACTGGCC
GTGGTGGATAACCTTTCCAGCAAGCTCTACCTCATCGTATATGGGGACGCCGCTCTTGAGGGCGCGTACTCCAGCGCCCG
GAAACGGTTGAAAGAACTGTTGGGATCTCTCCGGGAACCGTTACGGATTCCATTGGAAATGCCGTGTGAATCGGGTGCGC
CGGTTTCGCAGTTCGCGGAGGCGGATTTCATCTCGGCAGTAGAACGGGCCAAGCGCTATATTTTTGATGGCGATATCATG
CAGGTGGTGCTATCGCAGCGCACCAGCAAACCGTACGGCGCATCACCGCTCGCGCTGTACCGCGCGTTGCGCAGCCTCAA
TCCTTCTCCCTACATGTTTTATTACCACCTCGGCAGCTTTTACGTGGTGGGAGCCTCGCCCGAAATCCTGGTGCGACTCG
AAGGTGAAACGGTAACGGTACGCCCGATCGCAGGAACCCGTCCCCGGGGCAAGACCTTGGGGGAAGACGCTGCGCTGGCC
ACCGATCTGCTCGCCGACCCGAAAGAGCGGGCGGAGCATGTAATGCTGATGGACCTGGGGCGTAATGATGTAGGACGCGT
GGCGCAGATCGGAACGGTAAAGGTGACAGAGAACATGCGTATCGAGCACTACTCGCACGTCATGCATATTGTTTCCAATG
TCGAAGGCCGGCTCAAGCCCGGCCTCAATGCGATGGATGTGCTGCGCGCCACCTTTCCGGCGGGGACCGTAAGTGGCGCG
CCCAAGGTGCGGGCAATGGAAATCATCGATGAACTGGAAGTCTCGAAGCGTGGAATCTATGCAGGTGCAGTCGGATATCT
TGGATTCAACGGGGATATGGATCTGGCTATCGCCATCCGCACTGGAGTGATCAAGGACGGCAAACTGCATGTCCAGGCGG
GTGCGGGCATCGTTGCCGATTCCGTTCCGCAAAGCGAATGGGTGGAGACGCAAAACAAGGCCAGGGCCCTGCTGCGCGCG
GCGGAGATTGCGGAGAACGGGTTGGACAGCAGGATCGAATGA

Upstream 100 bases:

>100_bases
ATGGTGCTACTGGCACTGCTGGGGTTGGCGTTGGCGGCTCGATAAATATTGATCCGTTCTGCGGTATTTTTTCATTTTCT
GTTTTTTTGGAGTCTGAATC

Downstream 100 bases:

>100_bases
AAACCGGAAATAGCGCTAGCATGAATGCTTGACGTGAGGATGTTACGCGAGCACTTCGCTCCGTGGCGTGGCGAAGAGCG
CGAGGTAATGCTTGCAATCG

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTEAEFHALATKGYTHIPVVLETFADLDTPLSVYLKLANEPYSYLLESVQGGERFGRYSVIGLPARNRIEVRGNDVSVID
GSTSQVFQSEDPLAFVQSYLARFKAAPYPGLPRFCGGLAGYFAYDTVRYIERRLAGGCAYRLPDTLDTPDILLLVSEELA
VVDNLSSKLYLIVYGDAALEGAYSSARKRLKELLGSLREPLRIPLEMPCESGAPVSQFAEADFISAVERAKRYIFDGDIM
QVVLSQRTSKPYGASPLALYRALRSLNPSPYMFYYHLGSFYVVGASPEILVRLEGETVTVRPIAGTRPRGKTLGEDAALA
TDLLADPKERAEHVMLMDLGRNDVGRVAQIGTVKVTENMRIEHYSHVMHIVSNVEGRLKPGLNAMDVLRATFPAGTVSGA
PKVRAMEIIDELEVSKRGIYAGAVGYLGFNGDMDLAIAIRTGVIKDGKLHVQAGAGIVADSVPQSEWVETQNKARALLRA
AEIAENGLDSRIE

Sequences:

>Translated_493_residues
MTEAEFHALATKGYTHIPVVLETFADLDTPLSVYLKLANEPYSYLLESVQGGERFGRYSVIGLPARNRIEVRGNDVSVID
GSTSQVFQSEDPLAFVQSYLARFKAAPYPGLPRFCGGLAGYFAYDTVRYIERRLAGGCAYRLPDTLDTPDILLLVSEELA
VVDNLSSKLYLIVYGDAALEGAYSSARKRLKELLGSLREPLRIPLEMPCESGAPVSQFAEADFISAVERAKRYIFDGDIM
QVVLSQRTSKPYGASPLALYRALRSLNPSPYMFYYHLGSFYVVGASPEILVRLEGETVTVRPIAGTRPRGKTLGEDAALA
TDLLADPKERAEHVMLMDLGRNDVGRVAQIGTVKVTENMRIEHYSHVMHIVSNVEGRLKPGLNAMDVLRATFPAGTVSGA
PKVRAMEIIDELEVSKRGIYAGAVGYLGFNGDMDLAIAIRTGVIKDGKLHVQAGAGIVADSVPQSEWVETQNKARALLRA
AEIAENGLDSRIE
>Mature_492_residues
TEAEFHALATKGYTHIPVVLETFADLDTPLSVYLKLANEPYSYLLESVQGGERFGRYSVIGLPARNRIEVRGNDVSVIDG
STSQVFQSEDPLAFVQSYLARFKAAPYPGLPRFCGGLAGYFAYDTVRYIERRLAGGCAYRLPDTLDTPDILLLVSEELAV
VDNLSSKLYLIVYGDAALEGAYSSARKRLKELLGSLREPLRIPLEMPCESGAPVSQFAEADFISAVERAKRYIFDGDIMQ
VVLSQRTSKPYGASPLALYRALRSLNPSPYMFYYHLGSFYVVGASPEILVRLEGETVTVRPIAGTRPRGKTLGEDAALAT
DLLADPKERAEHVMLMDLGRNDVGRVAQIGTVKVTENMRIEHYSHVMHIVSNVEGRLKPGLNAMDVLRATFPAGTVSGAP
KVRAMEIIDELEVSKRGIYAGAVGYLGFNGDMDLAIAIRTGVIKDGKLHVQAGAGIVADSVPQSEWVETQNKARALLRAA
EIAENGLDSRIE

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=394, Percent_Identity=38.8324873096447, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1788114, Length=471, Percent_Identity=31.6348195329087, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1786809, Length=221, Percent_Identity=30.316742081448, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87082077, Length=266, Percent_Identity=27.4436090225564, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=463, Percent_Identity=40.8207343412527, Blast_Score=313, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6324361, Length=399, Percent_Identity=27.5689223057644, Blast_Score=133, Evalue=7e-32,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 53900; Mature: 53769

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAEFHALATKGYTHIPVVLETFADLDTPLSVYLKLANEPYSYLLESVQGGERFGRYSV
CCCCHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCHHCCCEEE
IGLPARNRIEVRGNDVSVIDGSTSQVFQSEDPLAFVQSYLARFKAAPYPGLPRFCGGLAG
EECCCCCEEEEECCEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
YFAYDTVRYIERRLAGGCAYRLPDTLDTPDILLLVSEELAVVDNLSSKLYLIVYGDAALE
HHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCHHHHHHCCCCCEEEEEECCHHHH
GAYSSARKRLKELLGSLREPLRIPLEMPCESGAPVSQFAEADFISAVERAKRYIFDGDIM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
QVVLSQRTSKPYGASPLALYRALRSLNPSPYMFYYHLGSFYVVGASPEILVRLEGETVTV
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEEEECCCEEEE
RPIAGTRPRGKTLGEDAALATDLLADPKERAEHVMLMDLGRNDVGRVAQIGTVKVTENMR
EECCCCCCCCCCCCCHHHHHHHHHCCCHHHHCEEEEEECCCCCCCCCEECCEEEEECCCC
IEHYSHVMHIVSNVEGRLKPGLNAMDVLRATFPAGTVSGAPKVRAMEIIDELEVSKRGIY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEE
AGAVGYLGFNGDMDLAIAIRTGVIKDGKLHVQAGAGIVADSVPQSEWVETQNKARALLRA
EEEEEEEEECCCCEEEEEEEECCEECCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHH
AEIAENGLDSRIE
HHHHHCCCCCCCC
>Mature Secondary Structure 
TEAEFHALATKGYTHIPVVLETFADLDTPLSVYLKLANEPYSYLLESVQGGERFGRYSV
CCCHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCHHCCCEEE
IGLPARNRIEVRGNDVSVIDGSTSQVFQSEDPLAFVQSYLARFKAAPYPGLPRFCGGLAG
EECCCCCEEEEECCEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
YFAYDTVRYIERRLAGGCAYRLPDTLDTPDILLLVSEELAVVDNLSSKLYLIVYGDAALE
HHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCHHHHHHCCCCCEEEEEECCHHHH
GAYSSARKRLKELLGSLREPLRIPLEMPCESGAPVSQFAEADFISAVERAKRYIFDGDIM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
QVVLSQRTSKPYGASPLALYRALRSLNPSPYMFYYHLGSFYVVGASPEILVRLEGETVTV
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEEEECCCEEEE
RPIAGTRPRGKTLGEDAALATDLLADPKERAEHVMLMDLGRNDVGRVAQIGTVKVTENMR
EECCCCCCCCCCCCCHHHHHHHHHCCCHHHHCEEEEEECCCCCCCCCEECCEEEEECCCC
IEHYSHVMHIVSNVEGRLKPGLNAMDVLRATFPAGTVSGAPKVRAMEIIDELEVSKRGIY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEE
AGAVGYLGFNGDMDLAIAIRTGVIKDGKLHVQAGAGIVADSVPQSEWVETQNKARALLRA
EEEEEEEEECCCCEEEEEEEECCEECCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHH
AEIAENGLDSRIE
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10417653 [H]