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The map label for this gene is argH

Identifier: 82703445

GI number: 82703445

Start: 2648715

End: 2650145

Strand: Direct

Name: argH

Synonym: Nmul_A2328

Alternate gene names: 82703445

Gene position: 2648715-2650145 (Clockwise)

Preceding gene: 82703444

Following gene: 82703446

Centisome position: 83.18

GC content: 56.6

Gene sequence:

>1431_bases
ATGACGGCCTCAAAAAGCACGGAAACCCTGCCCGGCGATAACACAAACACATGGTCAGGACGCTTTGATGAACCGGTTTC
AGAACTGGTTCAGCGGTACACAGCGTCGGTCGGTTTCGACAAGCGACTGGCAGAGTACGATATCCAGGGATCTCTCGCAC
ATGCGCGCATGCTCGCTGCGGGTGGCATTATCGGCCCTCACGACCTCGACGCAATCGAACGGGGCCTGAGCCAGATCCGG
CAGGAAATCCAGGGTGGCGGATTTGTCTGGCAGTTGGCTCTGGAGGACGTTCATCTCAATATCGAGAAGCGTCTGACTGC
GCTGATTGGTGACGCCGGGAAACGGCTGCACACGGCACGTTCCCGTAATGATCAAGTAGCGACGGACATACGGCTCTATT
TGCGCGCCTCCATCGACCGGATTACCGCCCTGATCCATGCAATGCAGAAGGCGTTGCTGCATCTGGCGGAGCAGCATGTC
GATACGGTGATGCCCGGCTTCACGCACCTGCAGGTCGCGCAACCCATCGTCTTCGGACACCATCTCATTGCCTATTTCGA
AATGCTGAAACGCGACGTGGAGCGTCTGGCCGACTGCAGAAAGCGCGTGAACAAACTTCCACTGGGAGCAGCCGCGCTGG
CAGGAACAAGCTATTCCGTCGATCGCGCGATGGTAGCGCAGGAACTGGGTTTCGAGGGTGTCTGCGAAAATTCGCTGGAT
GCCGTTTCCGATCGGGATTTTGCCATTGAATTCTGTGCAGCAGCAGCGCTGATCATGACGCATCTGTCGCGCCTGTCCGA
GGAACTCATCTTGTGGATGAGTCCACCTTTCGGATTTATCGACCTGGCTGACCGCTTCTGTACGGGGTCATCCATCATGC
CACAAAAGAAAAACCCGGATGTGCCCGAGCTGGTGCGCGGCAAAACCGGGCGTGTGAACGGACATCTGGTCGCGTTGCTG
ACTCTGATGAAGGCCCAGCCGCTCGCGTACAACAAGGATAACCAGGAAGACAAGGAGCCTTTGTTCGATACGGTAGATAC
ATTGACTGACACGCTGCGCATTTATGCCGATATGATGGCAGGTATACGCGTCAAGGAGACTGCGATGCGGGAAGCGGCGA
AGCGCGGGTACGCTACTGCTACCGATCTTGCCGATTATCTGACAAAAAAAGGATTGCCTTTCCGTGAAGCGCATGAAGCA
GTGGCACAAGCAGTGCGCTTCGCCGAAAAGAACAACCGGGACTTAAGCGCGCTCACCCTGCCAGAGCTACAGCAATTCTC
TCCGTTGATCGAGGACGATATCTTCACTGTGCTGACGCTGGAAGGGTCGTTAAATAGCCGCAATCACATTGGCGGTACTG
CCCCGGTACAGGTTGCCGCCGCTATACGGCGGGCGAGAGAATGGCTATCCGCAACCCCAGCCGCGCAATAG

Upstream 100 bases:

>100_bases
ACGGATGATTTGATTTATACTCGTCACTTTTTTCTGAAGCAACGATAGAAAGTGGTTTTTAAGGACCCCGGTCTTTGCAA
GCCATTCCATCGGGAACATT

Downstream 100 bases:

>100_bases
TCTGAAGCAATTATGCAATCCTGGACAGAAATCGCGGTGCAAATCTCCCATTCCACCGGTACCCCTTTTACCATACAAAC
AGCTTCTCCCATGGGTGGCG

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTASKSTETLPGDNTNTWSGRFDEPVSELVQRYTASVGFDKRLAEYDIQGSLAHARMLAAGGIIGPHDLDAIERGLSQIR
QEIQGGGFVWQLALEDVHLNIEKRLTALIGDAGKRLHTARSRNDQVATDIRLYLRASIDRITALIHAMQKALLHLAEQHV
DTVMPGFTHLQVAQPIVFGHHLIAYFEMLKRDVERLADCRKRVNKLPLGAAALAGTSYSVDRAMVAQELGFEGVCENSLD
AVSDRDFAIEFCAAAALIMTHLSRLSEELILWMSPPFGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALL
TLMKAQPLAYNKDNQEDKEPLFDTVDTLTDTLRIYADMMAGIRVKETAMREAAKRGYATATDLADYLTKKGLPFREAHEA
VAQAVRFAEKNNRDLSALTLPELQQFSPLIEDDIFTVLTLEGSLNSRNHIGGTAPVQVAAAIRRAREWLSATPAAQ

Sequences:

>Translated_476_residues
MTASKSTETLPGDNTNTWSGRFDEPVSELVQRYTASVGFDKRLAEYDIQGSLAHARMLAAGGIIGPHDLDAIERGLSQIR
QEIQGGGFVWQLALEDVHLNIEKRLTALIGDAGKRLHTARSRNDQVATDIRLYLRASIDRITALIHAMQKALLHLAEQHV
DTVMPGFTHLQVAQPIVFGHHLIAYFEMLKRDVERLADCRKRVNKLPLGAAALAGTSYSVDRAMVAQELGFEGVCENSLD
AVSDRDFAIEFCAAAALIMTHLSRLSEELILWMSPPFGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALL
TLMKAQPLAYNKDNQEDKEPLFDTVDTLTDTLRIYADMMAGIRVKETAMREAAKRGYATATDLADYLTKKGLPFREAHEA
VAQAVRFAEKNNRDLSALTLPELQQFSPLIEDDIFTVLTLEGSLNSRNHIGGTAPVQVAAAIRRAREWLSATPAAQ
>Mature_475_residues
TASKSTETLPGDNTNTWSGRFDEPVSELVQRYTASVGFDKRLAEYDIQGSLAHARMLAAGGIIGPHDLDAIERGLSQIRQ
EIQGGGFVWQLALEDVHLNIEKRLTALIGDAGKRLHTARSRNDQVATDIRLYLRASIDRITALIHAMQKALLHLAEQHVD
TVMPGFTHLQVAQPIVFGHHLIAYFEMLKRDVERLADCRKRVNKLPLGAAALAGTSYSVDRAMVAQELGFEGVCENSLDA
VSDRDFAIEFCAAAALIMTHLSRLSEELILWMSPPFGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALLT
LMKAQPLAYNKDNQEDKEPLFDTVDTLTDTLRIYADMMAGIRVKETAMREAAKRGYATATDLADYLTKKGLPFREAHEAV
AQAVRFAEKNNRDLSALTLPELQQFSPLIEDDIFTVLTLEGSLNSRNHIGGTAPVQVAAAIRRAREWLSATPAAQ

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=465, Percent_Identity=41.9354838709677, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI68303542, Length=465, Percent_Identity=41.9354838709677, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI68303547, Length=465, Percent_Identity=40, Blast_Score=351, Evalue=8e-97,
Organism=Homo sapiens, GI68303549, Length=465, Percent_Identity=38.9247311827957, Blast_Score=338, Evalue=8e-93,
Organism=Escherichia coli, GI1790398, Length=446, Percent_Identity=46.1883408071749, Blast_Score=410, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321806, Length=444, Percent_Identity=41.6666666666667, Blast_Score=339, Evalue=7e-94,
Organism=Drosophila melanogaster, GI221473854, Length=454, Percent_Identity=40.0881057268723, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI78706858, Length=454, Percent_Identity=40.0881057268723, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_NITMU (Q2Y6K2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413011.1
- ProteinModelPortal:   Q2Y6K2
- SMR:   Q2Y6K2
- STRING:   Q2Y6K2
- GeneID:   3785318
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2328
- eggNOG:   COG0165
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- PhylomeDB:   Q2Y6K2
- ProtClustDB:   PRK00855
- BioCyc:   NMUL323848:NMUL_A2328-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 52524; Mature: 52392

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASKSTETLPGDNTNTWSGRFDEPVSELVQRYTASVGFDKRLAEYDIQGSLAHARMLAA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
GGIIGPHDLDAIERGLSQIRQEIQGGGFVWQLALEDVHLNIEKRLTALIGDAGKRLHTAR
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRNDQVATDIRLYLRASIDRITALIHAMQKALLHLAEQHVDTVMPGFTHLQVAQPIVFGH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HLIAYFEMLKRDVERLADCRKRVNKLPLGAAALAGTSYSVDRAMVAQELGFEGVCENSLD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHH
AVSDRDFAIEFCAAAALIMTHLSRLSEELILWMSPPFGFIDLADRFCTGSSIMPQKKNPD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCCCCC
VPELVRGKTGRVNGHLVALLTLMKAQPLAYNKDNQEDKEPLFDTVDTLTDTLRIYADMMA
CHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIRVKETAMREAAKRGYATATDLADYLTKKGLPFREAHEAVAQAVRFAEKNNRDLSALTL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECC
PELQQFSPLIEDDIFTVLTLEGSLNSRNHIGGTAPVQVAAAIRRAREWLSATPAAQ
HHHHHHCCHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TASKSTETLPGDNTNTWSGRFDEPVSELVQRYTASVGFDKRLAEYDIQGSLAHARMLAA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
GGIIGPHDLDAIERGLSQIRQEIQGGGFVWQLALEDVHLNIEKRLTALIGDAGKRLHTAR
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRNDQVATDIRLYLRASIDRITALIHAMQKALLHLAEQHVDTVMPGFTHLQVAQPIVFGH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HLIAYFEMLKRDVERLADCRKRVNKLPLGAAALAGTSYSVDRAMVAQELGFEGVCENSLD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHH
AVSDRDFAIEFCAAAALIMTHLSRLSEELILWMSPPFGFIDLADRFCTGSSIMPQKKNPD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCCCCC
VPELVRGKTGRVNGHLVALLTLMKAQPLAYNKDNQEDKEPLFDTVDTLTDTLRIYADMMA
CHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIRVKETAMREAAKRGYATATDLADYLTKKGLPFREAHEAVAQAVRFAEKNNRDLSALTL
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECC
PELQQFSPLIEDDIFTVLTLEGSLNSRNHIGGTAPVQVAAAIRRAREWLSATPAAQ
HHHHHHCCHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA