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The map label for this gene is priA [H]

Identifier: 82703444

GI number: 82703444

Start: 2646354

End: 2648558

Strand: Direct

Name: priA [H]

Synonym: Nmul_A2327

Alternate gene names: 82703444

Gene position: 2646354-2648558 (Clockwise)

Preceding gene: 82703436

Following gene: 82703445

Centisome position: 83.11

GC content: 55.28

Gene sequence:

>2205_bases
ATGTCCATCATTCGCGTTGCGCTTGATAAGCCTTTGAATACACTTTTCGATTATCTCGGCCCGGCAGGAGTAACAGAGCA
GGATATCGGTTGTCGCGTATTCGTTCCCTTTGGAAAAAGTCGCATGACGGGGGTTATCATGGAGGTAGCCTCTTACTCCG
ACATTTCCCCGGACAAGATCAAGCAAGCCGGTCAGGTATTCCGCGATGTTCCACCCCTCCCCCCGGAGTTGCTCGATTTA
TTCCGTTTTTGCAGCGAGTATTACCACCACCCCCTGGGAGAAGTGGTAATGAATGGCCTACCAATCAGATTGCGCGGCAG
TCAACCTTTTTTCATTCGCACGGCTCCCATCGTTTTTCAATATCGCCTCACGACGAAGGGTCGCGCTGTCGATATATCGG
CCATTCCGGGTCGCAGCATCGCCAAACGTAGACTTCTGGTAAAGCTGAAGGAGTCCGGGATGATCGAAGATGGAGAGGCA
AAGCAATTTTCCAGCCGAACCTCCGAGGCCATGAAGGAGTTCGTCGGTTTGCAATGGGTGGAAGAAATACCTCTTGCGCC
CCCTGTGGTTTCGACGTCTCCGTCTCAAGAAACATCCCCGCTCCCCGTCCTGACCCCCGCGCAGAAAAATGCGATCGATG
CAATAGTCCCGAAAATGGGGGAATTCCATACATGGCTATTGCATGGAGTGACGGGCAGCGGAAAAACGGAGATATACCTG
CAATTGATTTCACTGCTGCTGCGCCAGGCACGACAGACGCTCGTCCTCGTCCCGGAGATCAACCTGACGCCACAATTGGA
GCTCATCTTCCGCGCTCGCTTTCCCGGAGTAAGGCTGGTCAGCCTCCATAGCGGATTAAATCCCACTGAACGCGCCGAAG
GATGGGTCCAGGCGCAGCATGGAGAAGCCGAAATAATACTGGGTACCCGGCTCGCTGTATTTACTCCGCTCCCAAGGCTG
GGAATGATCATCGTGGATGAGGAGCACGATCTCTCATTCAAACAGCAGGAGGGTTTGCGCTACTCGGCGCGGGATGTCGC
TGTCTTTCGTGCAAAGCGCGCCGGAATACCCATAGTATTGGGTTCGGCCACTCCGTCACTCGAAACCTATTACAACGCCA
TTACAGGACGCTATCTCCTGTTGCGCCTGCCCTCGCGCGCTCCCGACAATGCCTCCCTGCCCACGGTGCAGTATATCGAT
ATCGGTAAAATAAAAACCCAGGAGGGCTTTTCCCCTCCGGTCATCGCCGCGCTGGAAAAAGGTCTGGCATCAAACCAGCA
AAGCCTGGTTTTTATAAACCGTCGTGGTTACGCGCCAATTCTGCTATGCAGGGCATGCGGGTGGACAGCGCTCTGCCATC
GCTGCTCTAGCCGTCTGGTTGTCCACTTGCGCGAAAAAAAACTGCGCTGTCACCATTGCGGCCATCAAGCGGAATTTCCC
AGCCTCTGTCCGGAATGCGGGAATCAGGATATTGCTCCTTTTGGTCACGGCACACAACGAATCGAAGCTGCATTGACCCG
GCTTTTTCCCGATGCCCGCATCGCCCGGATAGATCGCGACAGCACGAGGCGGAAAGATTCCTGGCAGCGGATATTGAAAG
ATATTCGTGAGCAACGCGTGGACATACTCGTAGGCACGCAGATTCTCGCGAAAGGACACGATTTTCCAAATCTATCGCTG
GTCAGTATTCTGAATTCCGACGCCTCCCTGTTCAGCACCGATTTCCGCGCATCGGAGCGATTGTTTGCCCAATTGATGCA
GGTAGCCGGACGCGCGGGCCGTTCAGGTACCCCGGGGGAAGTTTTGATTCAAACCCGGTTCCCCGATCACCCGCTCTACC
GCGCCCTGTACCAGCACGATTACGACGCGCTTGCCCGGATTCTGCTGGAAGAGAGGAAAACCGCGCATTTCCCGCCATTC
GTCTATCAGGCTCTATTACGGGCGGAGGCGCCGGCAATCGCTACCACACTCGCCTTTCTTGCCAAGGCGGCAACCTTGGC
AAAAGCGCCACACCAGATTAAGGTATTTGATCCGGTTCCGGCCCAAATGGCGCGGCTGAAGGGAATGGAGCGCGCTTATC
TGCTGGTGCAGTCAAGGTCCAGAAAGAAGCTGCAGGAATTCCTCGGGAACTGGCGCACGAAGCTCGACAGCCTTCCCAGC
CATAAAGTGCGCTGGACGCTGGACATCGACCCGCTGGAATTCTAG

Upstream 100 bases:

>100_bases
GGCCAGTCTGTATCCATAATTTGTACATCCCGCAGGATTCGTATTTCATCCTGACCGCGTTTATCATACCCCTTTCCACC
CCGATCACCGCTGATCTGGC

Downstream 100 bases:

>100_bases
GAATATCTGCATAGGGTTCCTCTGGAACATTCCCGAGGCAGGGCGGCGTCATGGGGACGGATGATTTGATTTATACTCGT
CACTTTTTTCTGAAGCAACG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MSIIRVALDKPLNTLFDYLGPAGVTEQDIGCRVFVPFGKSRMTGVIMEVASYSDISPDKIKQAGQVFRDVPPLPPELLDL
FRFCSEYYHHPLGEVVMNGLPIRLRGSQPFFIRTAPIVFQYRLTTKGRAVDISAIPGRSIAKRRLLVKLKESGMIEDGEA
KQFSSRTSEAMKEFVGLQWVEEIPLAPPVVSTSPSQETSPLPVLTPAQKNAIDAIVPKMGEFHTWLLHGVTGSGKTEIYL
QLISLLLRQARQTLVLVPEINLTPQLELIFRARFPGVRLVSLHSGLNPTERAEGWVQAQHGEAEIILGTRLAVFTPLPRL
GMIIVDEEHDLSFKQQEGLRYSARDVAVFRAKRAGIPIVLGSATPSLETYYNAITGRYLLLRLPSRAPDNASLPTVQYID
IGKIKTQEGFSPPVIAALEKGLASNQQSLVFINRRGYAPILLCRACGWTALCHRCSSRLVVHLREKKLRCHHCGHQAEFP
SLCPECGNQDIAPFGHGTQRIEAALTRLFPDARIARIDRDSTRRKDSWQRILKDIREQRVDILVGTQILAKGHDFPNLSL
VSILNSDASLFSTDFRASERLFAQLMQVAGRAGRSGTPGEVLIQTRFPDHPLYRALYQHDYDALARILLEERKTAHFPPF
VYQALLRAEAPAIATTLAFLAKAATLAKAPHQIKVFDPVPAQMARLKGMERAYLLVQSRSRKKLQEFLGNWRTKLDSLPS
HKVRWTLDIDPLEF

Sequences:

>Translated_734_residues
MSIIRVALDKPLNTLFDYLGPAGVTEQDIGCRVFVPFGKSRMTGVIMEVASYSDISPDKIKQAGQVFRDVPPLPPELLDL
FRFCSEYYHHPLGEVVMNGLPIRLRGSQPFFIRTAPIVFQYRLTTKGRAVDISAIPGRSIAKRRLLVKLKESGMIEDGEA
KQFSSRTSEAMKEFVGLQWVEEIPLAPPVVSTSPSQETSPLPVLTPAQKNAIDAIVPKMGEFHTWLLHGVTGSGKTEIYL
QLISLLLRQARQTLVLVPEINLTPQLELIFRARFPGVRLVSLHSGLNPTERAEGWVQAQHGEAEIILGTRLAVFTPLPRL
GMIIVDEEHDLSFKQQEGLRYSARDVAVFRAKRAGIPIVLGSATPSLETYYNAITGRYLLLRLPSRAPDNASLPTVQYID
IGKIKTQEGFSPPVIAALEKGLASNQQSLVFINRRGYAPILLCRACGWTALCHRCSSRLVVHLREKKLRCHHCGHQAEFP
SLCPECGNQDIAPFGHGTQRIEAALTRLFPDARIARIDRDSTRRKDSWQRILKDIREQRVDILVGTQILAKGHDFPNLSL
VSILNSDASLFSTDFRASERLFAQLMQVAGRAGRSGTPGEVLIQTRFPDHPLYRALYQHDYDALARILLEERKTAHFPPF
VYQALLRAEAPAIATTLAFLAKAATLAKAPHQIKVFDPVPAQMARLKGMERAYLLVQSRSRKKLQEFLGNWRTKLDSLPS
HKVRWTLDIDPLEF
>Mature_733_residues
SIIRVALDKPLNTLFDYLGPAGVTEQDIGCRVFVPFGKSRMTGVIMEVASYSDISPDKIKQAGQVFRDVPPLPPELLDLF
RFCSEYYHHPLGEVVMNGLPIRLRGSQPFFIRTAPIVFQYRLTTKGRAVDISAIPGRSIAKRRLLVKLKESGMIEDGEAK
QFSSRTSEAMKEFVGLQWVEEIPLAPPVVSTSPSQETSPLPVLTPAQKNAIDAIVPKMGEFHTWLLHGVTGSGKTEIYLQ
LISLLLRQARQTLVLVPEINLTPQLELIFRARFPGVRLVSLHSGLNPTERAEGWVQAQHGEAEIILGTRLAVFTPLPRLG
MIIVDEEHDLSFKQQEGLRYSARDVAVFRAKRAGIPIVLGSATPSLETYYNAITGRYLLLRLPSRAPDNASLPTVQYIDI
GKIKTQEGFSPPVIAALEKGLASNQQSLVFINRRGYAPILLCRACGWTALCHRCSSRLVVHLREKKLRCHHCGHQAEFPS
LCPECGNQDIAPFGHGTQRIEAALTRLFPDARIARIDRDSTRRKDSWQRILKDIREQRVDILVGTQILAKGHDFPNLSLV
SILNSDASLFSTDFRASERLFAQLMQVAGRAGRSGTPGEVLIQTRFPDHPLYRALYQHDYDALARILLEERKTAHFPPFV
YQALLRAEAPAIATTLAFLAKAATLAKAPHQIKVFDPVPAQMARLKGMERAYLLVQSRSRKKLQEFLGNWRTKLDSLPSH
KVRWTLDIDPLEF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=745, Percent_Identity=41.6107382550336, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82159; Mature: 82028

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIRVALDKPLNTLFDYLGPAGVTEQDIGCRVFVPFGKSRMTGVIMEVASYSDISPDKI
CCEEEEHHCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHH
KQAGQVFRDVPPLPPELLDLFRFCSEYYHHPLGEVVMNGLPIRLRGSQPFFIRTAPIVFQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCEEEEECCEEEE
YRLTTKGRAVDISAIPGRSIAKRRLLVKLKESGMIEDGEAKQFSSRTSEAMKEFVGLQWV
EEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCHHHH
EEIPLAPPVVSTSPSQETSPLPVLTPAQKNAIDAIVPKMGEFHTWLLHGVTGSGKTEIYL
HHCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCHHEEEEEECCCCCCHHHHH
QLISLLLRQARQTLVLVPEINLTPQLELIFRARFPGVRLVSLHSGLNPTERAEGWVQAQH
HHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHCCCEEECC
GEAEIILGTRLAVFTPLPRLGMIIVDEEHDLSFKQQEGLRYSARDVAVFRAKRAGIPIVL
CCEEEEEECHHHHHCCCCCCCEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCEEE
GSATPSLETYYNAITGRYLLLRLPSRAPDNASLPTVQYIDIGKIKTQEGFSPPVIAALEK
CCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCEEEEEECCCEECCCCCCCHHHHHHHH
GLASNQQSLVFINRRGYAPILLCRACGWTALCHRCSSRLVVHLREKKLRCHHCGHQAEFP
HHCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCCCC
SLCPECGNQDIAPFGHGTQRIEAALTRLFPDARIARIDRDSTRRKDSWQRILKDIREQRV
HHCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHH
DILVGTQILAKGHDFPNLSLVSILNSDASLFSTDFRASERLFAQLMQVAGRAGRSGTPGE
HHHCCHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VLIQTRFPDHPLYRALYQHDYDALARILLEERKTAHFPPFVYQALLRAEAPAIATTLAFL
EEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
AKAATLAKAPHQIKVFDPVPAQMARLKGMERAYLLVQSRSRKKLQEFLGNWRTKLDSLPS
HHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HKVRWTLDIDPLEF
CCEEEEEECCCCCC
>Mature Secondary Structure 
SIIRVALDKPLNTLFDYLGPAGVTEQDIGCRVFVPFGKSRMTGVIMEVASYSDISPDKI
CEEEEHHCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHH
KQAGQVFRDVPPLPPELLDLFRFCSEYYHHPLGEVVMNGLPIRLRGSQPFFIRTAPIVFQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCEEEEECCEEEE
YRLTTKGRAVDISAIPGRSIAKRRLLVKLKESGMIEDGEAKQFSSRTSEAMKEFVGLQWV
EEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCHHHH
EEIPLAPPVVSTSPSQETSPLPVLTPAQKNAIDAIVPKMGEFHTWLLHGVTGSGKTEIYL
HHCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCHHEEEEEECCCCCCHHHHH
QLISLLLRQARQTLVLVPEINLTPQLELIFRARFPGVRLVSLHSGLNPTERAEGWVQAQH
HHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHCCCEEECC
GEAEIILGTRLAVFTPLPRLGMIIVDEEHDLSFKQQEGLRYSARDVAVFRAKRAGIPIVL
CCEEEEEECHHHHHCCCCCCCEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCEEE
GSATPSLETYYNAITGRYLLLRLPSRAPDNASLPTVQYIDIGKIKTQEGFSPPVIAALEK
CCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCEEEEEECCCEECCCCCCCHHHHHHHH
GLASNQQSLVFINRRGYAPILLCRACGWTALCHRCSSRLVVHLREKKLRCHHCGHQAEFP
HHCCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCCCC
SLCPECGNQDIAPFGHGTQRIEAALTRLFPDARIARIDRDSTRRKDSWQRILKDIREQRV
HHCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHH
DILVGTQILAKGHDFPNLSLVSILNSDASLFSTDFRASERLFAQLMQVAGRAGRSGTPGE
HHHCCHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VLIQTRFPDHPLYRALYQHDYDALARILLEERKTAHFPPFVYQALLRAEAPAIATTLAFL
EEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
AKAATLAKAPHQIKVFDPVPAQMARLKGMERAYLLVQSRSRKKLQEFLGNWRTKLDSLPS
HHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HKVRWTLDIDPLEF
CCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]