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The map label for this gene is prfB [H]

Identifier: 82703392

GI number: 82703392

Start: 2585912

End: 2586844

Strand: Direct

Name: prfB [H]

Synonym: Nmul_A2274

Alternate gene names: 82703392

Gene position: 2585912-2586844 (Clockwise)

Preceding gene: 82703391

Following gene: 82703393

Centisome position: 81.21

GC content: 54.66

Gene sequence:

>933_bases
TTGGGGCGCGAAAAAAAAATCCTGGAAAGCGTGGTCATCAACCTGGAAACAATTTCCCGCCAGCTACAGGATGCGCGGGA
GCTGTTCGAGATGGCGGAAGAAGAGGATGACGATGCCACTCTTCAAAGCGTGGCGCGCGACATCAGTCATTTGGAAAAAG
CAGTGTCGGACATGGAATTCCGACGCATGTTTTCCAATCCCATGGATACGAATAACTGCTTCGTGGATATCCAGTCGGGC
TCGGGCGGCACTGAAGCGCAGGACTGGGCTGCCATGTTGGAGCGCATGTACTTGCGTTATTGCGAGCGCCGCGGTTACCA
GGTGGAAGTAATGGAAGAATCGCCGGGAGAAGTGGCGGGAATCAAGAGCGCGAGCCTCAAAATATCGGGGGAGTATGCCT
ATGGCTACCTTCGGACCGAAACCGGCGTTCACCGCCTTGTCCGCAAATCCCCATTCGATTCCGGCAATCGCCGTCACACC
TCGTTTGCAAGCGTATTCGTCTATCCGGAGGTGGATGAAACCATCGAAATCGACATTAACCCGGCGGACCTGCGGGTTGA
TACCTTTCGTGCTTCCGGGGCGGGTGGACAGCACATCAACAAGACTGACTCGGCAGTCCGGATTACACATAACCCTACCG
GTATCGTCGTTCAATGCCAAAGCGGTCGTTCCCAGCATCGTAATCGCGCGGATGCGATGGCGATGCTGAAATCGCGCCTT
TATGAAGCGGAACTGCGCAAACGCAATGAGGAAAAGCAGGCGATAGAAGATACGAAAACCGATATCGGCTGGGGACATCA
GATCCGCTCCTATGTGTTGGACCAGGCGCGCATCAAGGACCTGCGAACGGGGGTGGAAAGCGGCAACACCCAGGCTATCC
TCGACGGGGATCTGGATGATTTTATCGAAGCAAGCTTGAAACAAGGCGTATAA

Upstream 100 bases:

>100_bases
AGGTATCTTTGACTTCGATACCCAGAAAACACGCCTGACCGAGCTTAACCGGCTGACGGAAGATCCCGCTGTCTGGGATG
ACAACAAGCGTGCCCAGGAA

Downstream 100 bases:

>100_bases
AAGGACCGATCGGAGATGGATGAGGCTTTAGAGAAAGATTCGGAGGAAACGAATCGGTTTCAGGCAGAGATCAGGCTCGT
CGAGGAGCGGCGAGCAAAAC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MGREKKILESVVINLETISRQLQDARELFEMAEEEDDDATLQSVARDISHLEKAVSDMEFRRMFSNPMDTNNCFVDIQSG
SGGTEAQDWAAMLERMYLRYCERRGYQVEVMEESPGEVAGIKSASLKISGEYAYGYLRTETGVHRLVRKSPFDSGNRRHT
SFASVFVYPEVDETIEIDINPADLRVDTFRASGAGGQHINKTDSAVRITHNPTGIVVQCQSGRSQHRNRADAMAMLKSRL
YEAELRKRNEEKQAIEDTKTDIGWGHQIRSYVLDQARIKDLRTGVESGNTQAILDGDLDDFIEASLKQGV

Sequences:

>Translated_310_residues
MGREKKILESVVINLETISRQLQDARELFEMAEEEDDDATLQSVARDISHLEKAVSDMEFRRMFSNPMDTNNCFVDIQSG
SGGTEAQDWAAMLERMYLRYCERRGYQVEVMEESPGEVAGIKSASLKISGEYAYGYLRTETGVHRLVRKSPFDSGNRRHT
SFASVFVYPEVDETIEIDINPADLRVDTFRASGAGGQHINKTDSAVRITHNPTGIVVQCQSGRSQHRNRADAMAMLKSRL
YEAELRKRNEEKQAIEDTKTDIGWGHQIRSYVLDQARIKDLRTGVESGNTQAILDGDLDDFIEASLKQGV
>Mature_309_residues
GREKKILESVVINLETISRQLQDARELFEMAEEEDDDATLQSVARDISHLEKAVSDMEFRRMFSNPMDTNNCFVDIQSGS
GGTEAQDWAAMLERMYLRYCERRGYQVEVMEESPGEVAGIKSASLKISGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTS
FASVFVYPEVDETIEIDINPADLRVDTFRASGAGGQHINKTDSAVRITHNPTGIVVQCQSGRSQHRNRADAMAMLKSRLY
EAELRKRNEEKQAIEDTKTDIGWGHQIRSYVLDQARIKDLRTGVESGNTQAILDGDLDDFIEASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=312, Percent_Identity=34.6153846153846, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI34577120, Length=316, Percent_Identity=33.8607594936709, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=35.5450236966825, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI2367172, Length=310, Percent_Identity=64.8387096774194, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=45.3441295546559, Blast_Score=207, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=237, Percent_Identity=31.2236286919831, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=273, Percent_Identity=35.8974358974359, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19921226, Length=227, Percent_Identity=40.9691629955947, Blast_Score=154, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 34979; Mature: 34848

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGREKKILESVVINLETISRQLQDARELFEMAEEEDDDATLQSVARDISHLEKAVSDMEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRMFSNPMDTNNCFVDIQSGSGGTEAQDWAAMLERMYLRYCERRGYQVEVMEESPGEVAG
HHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEC
IKSASLKISGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTSFASVFVYPEVDETIEIDIN
CCCCEEEEECCEEEEEEECHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEECC
PADLRVDTFRASGAGGQHINKTDSAVRITHNPTGIVVQCQSGRSQHRNRADAMAMLKSRL
HHHEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
YEAELRKRNEEKQAIEDTKTDIGWGHQIRSYVLDQARIKDLRTGVESGNTQAILDGDLDD
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
FIEASLKQGV
HHHHHHHHCC
>Mature Secondary Structure 
GREKKILESVVINLETISRQLQDARELFEMAEEEDDDATLQSVARDISHLEKAVSDMEF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRMFSNPMDTNNCFVDIQSGSGGTEAQDWAAMLERMYLRYCERRGYQVEVMEESPGEVAG
HHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEC
IKSASLKISGEYAYGYLRTETGVHRLVRKSPFDSGNRRHTSFASVFVYPEVDETIEIDIN
CCCCEEEEECCEEEEEEECHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEEECC
PADLRVDTFRASGAGGQHINKTDSAVRITHNPTGIVVQCQSGRSQHRNRADAMAMLKSRL
HHHEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
YEAELRKRNEEKQAIEDTKTDIGWGHQIRSYVLDQARIKDLRTGVESGNTQAILDGDLDD
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
FIEASLKQGV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA