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The map label for this gene is greB [H]

Identifier: 82702992

GI number: 82702992

Start: 2157406

End: 2158047

Strand: Reverse

Name: greB [H]

Synonym: Nmul_A1871

Alternate gene names: 82702992

Gene position: 2158047-2157406 (Counterclockwise)

Preceding gene: 82702993

Following gene: 82702991

Centisome position: 67.77

GC content: 53.58

Gene sequence:

>642_bases
ATGAGCAAGGCATTTACCAGGGAAAGCGAGTCTGACGACCTGGACGAAGAAGGCAACGGGAATGGCCTGCCATCATTACC
TTCCGGCTCCAAAAATTACATTACTCCGGAGGGGCATAAGCGGCTGATGGACGAGTTTTTGTGGTTGATGAACAGGGAAC
GTCCCCAGGTAACCGCAACGGTTTCCTGGGCTGCCGCGAATGGCGATCGTTCGGAGAACGCGGATTATATCTACGGCAAG
AAGCGCCTGCGCGAAATCGATCGGCGCATTCGTTTTCTCACCCGACGACTGGATATGGCGGAAATCATCGATCCCTCCAT
GCCCCGGGAAGATGAATCGCGGATTTTTTTTGGCGCTACCGTTACCTACATTAACGAGAAAGGCGAGGAGAAGATCGTTT
CCATCGTGGGTGTGAATGAGATCGATACCACCAGGGGCTACATAAGCTGGGTTTCACCACTGGCGCGGACATTGCTCAAA
GCGCGTGAAGGGGATGTGGTGACATTACATGCGCCCGGAGGAACGGAAGAACTGGAAATTCTGGAAGTCAGGTATCAGGC
AATTCCCATGGAACCGTTCAGGATGGGCTCGGGAATGGAGAAGACGATGGCGAACCCTGGCGAGAACCCCAATCCTCAAT
GA

Upstream 100 bases:

>100_bases
CTGGATCTGGCCTGGACCTGCTCAAACAGGAGGCATTGTGCCTGCCTGTCAAGATCGGATTCCGATTGCTGACCGATTGA
ATGAGATTGAATGAGCAAGC

Downstream 100 bases:

>100_bases
AGTTTATTACCTCTCGCGACAATCCCACCTTCAAAGAACTGGTTAAACTGCGGGAATCATCCCGGCAACGGCGAATTGCG
GGACTGACGCTTCTTGACGG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSKAFTRESESDDLDEEGNGNGLPSLPSGSKNYITPEGHKRLMDEFLWLMNRERPQVTATVSWAAANGDRSENADYIYGK
KRLREIDRRIRFLTRRLDMAEIIDPSMPREDESRIFFGATVTYINEKGEEKIVSIVGVNEIDTTRGYISWVSPLARTLLK
AREGDVVTLHAPGGTEELEILEVRYQAIPMEPFRMGSGMEKTMANPGENPNPQ

Sequences:

>Translated_213_residues
MSKAFTRESESDDLDEEGNGNGLPSLPSGSKNYITPEGHKRLMDEFLWLMNRERPQVTATVSWAAANGDRSENADYIYGK
KRLREIDRRIRFLTRRLDMAEIIDPSMPREDESRIFFGATVTYINEKGEEKIVSIVGVNEIDTTRGYISWVSPLARTLLK
AREGDVVTLHAPGGTEELEILEVRYQAIPMEPFRMGSGMEKTMANPGENPNPQ
>Mature_212_residues
SKAFTRESESDDLDEEGNGNGLPSLPSGSKNYITPEGHKRLMDEFLWLMNRERPQVTATVSWAAANGDRSENADYIYGKK
RLREIDRRIRFLTRRLDMAEIIDPSMPREDESRIFFGATVTYINEKGEEKIVSIVGVNEIDTTRGYISWVSPLARTLLKA
REGDVVTLHAPGGTEELEILEVRYQAIPMEPFRMGSGMEKTMANPGENPNPQ

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=46.7105263157895, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=36.3636363636364, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 24097; Mature: 23966

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAFTRESESDDLDEEGNGNGLPSLPSGSKNYITPEGHKRLMDEFLWLMNRERPQVTAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
VSWAAANGDRSENADYIYGKKRLREIDRRIRFLTRRLDMAEIIDPSMPREDESRIFFGAT
EEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEEEEE
VTYINEKGEEKIVSIVGVNEIDTTRGYISWVSPLARTLLKAREGDVVTLHAPGGTEELEI
EEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
LEVRYQAIPMEPFRMGSGMEKTMANPGENPNPQ
EEEHHHHCCCCHHHCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
SKAFTRESESDDLDEEGNGNGLPSLPSGSKNYITPEGHKRLMDEFLWLMNRERPQVTAT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
VSWAAANGDRSENADYIYGKKRLREIDRRIRFLTRRLDMAEIIDPSMPREDESRIFFGAT
EEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEEEEE
VTYINEKGEEKIVSIVGVNEIDTTRGYISWVSPLARTLLKAREGDVVTLHAPGGTEELEI
EEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
LEVRYQAIPMEPFRMGSGMEKTMANPGENPNPQ
EEEHHHHCCCCHHHCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]