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The map label for this gene is ampD [H]

Identifier: 82702935

GI number: 82702935

Start: 2073561

End: 2074106

Strand: Reverse

Name: ampD [H]

Synonym: Nmul_A1812

Alternate gene names: 82702935

Gene position: 2074106-2073561 (Counterclockwise)

Preceding gene: 82702936

Following gene: 82702933

Centisome position: 65.14

GC content: 57.69

Gene sequence:

>546_bases
ATGCAGATCGATGCCACGGGATGTGCGAGGGGCATCCGCTTCATCGCTTCGCCCAATTTCGACGAACGGCCGGGGGGATG
TGCGATTTCACTGCTCGTGATTCACAACATCAGCCTTCCTCCCGATCAGTTTGGCGGAAACGGTGTAGCCGAGTTATTCA
CCAACGAGCTCGATCCGGAAGCCCACCCCTACTACCGCACCATTCACGGCCTCAGGGTTTCCAGTCACTTTTATATTCCC
CGGACAGGAGAGGTCATTCAATTCGTCCCGTGCGAAAAACGCGCCTGGCATGCAGGGGAGTCCTGCTGGCAGGGCAGGAG
CCGGTGCAATGACTTTTCCATCGGTATCGAGCTCGAAGGCAGCGACTCGCAGCCTTTCACCGATTCTCAATACAGAATGC
TGGTCGGGCTCACGCTGGAACTGCAAAAGGCTTATCCCATCGAGCATATTGCAGGGCATTCCGACATTGCCCCGGGTCGC
AAGACCGACCCGGGCCCCTGTTTCGACTGGAAGCGTTACCGTACCGCCCTCGCTGCTTCTCGTTGA

Upstream 100 bases:

>100_bases
ATCGGCGACTGCACCTGCAATCTGAATCGTGAACCCGCCGGTGCAAATGGGTTACAGCCGGCAGAATGACGCGGGCGGCG
TCCGACAGGATGACTGCCTC

Downstream 100 bases:

>100_bases
GTCTTATTTCGAGTCTTGCGCAGTCTCTCCGGTAAGCATCAGAAAGAAGAACCCATCCTGTCATCTGGATGAGGTGCGCG
CTTTTTCCTTGGCCGCGGTA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MQIDATGCARGIRFIASPNFDERPGGCAISLLVIHNISLPPDQFGGNGVAELFTNELDPEAHPYYRTIHGLRVSSHFYIP
RTGEVIQFVPCEKRAWHAGESCWQGRSRCNDFSIGIELEGSDSQPFTDSQYRMLVGLTLELQKAYPIEHIAGHSDIAPGR
KTDPGPCFDWKRYRTALAASR

Sequences:

>Translated_181_residues
MQIDATGCARGIRFIASPNFDERPGGCAISLLVIHNISLPPDQFGGNGVAELFTNELDPEAHPYYRTIHGLRVSSHFYIP
RTGEVIQFVPCEKRAWHAGESCWQGRSRCNDFSIGIELEGSDSQPFTDSQYRMLVGLTLELQKAYPIEHIAGHSDIAPGR
KTDPGPCFDWKRYRTALAASR
>Mature_181_residues
MQIDATGCARGIRFIASPNFDERPGGCAISLLVIHNISLPPDQFGGNGVAELFTNELDPEAHPYYRTIHGLRVSSHFYIP
RTGEVIQFVPCEKRAWHAGESCWQGRSRCNDFSIGIELEGSDSQPFTDSQYRMLVGLTLELQKAYPIEHIAGHSDIAPGR
KTDPGPCFDWKRYRTALAASR

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=174, Percent_Identity=54.0229885057471, Blast_Score=196, Evalue=8e-52,
Organism=Escherichia coli, GI1787092, Length=124, Percent_Identity=39.5161290322581, Blast_Score=87, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20165; Mature: 20165

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIDATGCARGIRFIASPNFDERPGGCAISLLVIHNISLPPDQFGGNGVAELFTNELDPE
CEECCHHHHHHEEEEECCCCCCCCCCEEEEEEEEECCCCCCHHCCCCCHHHHHHCCCCCC
AHPYYRTIHGLRVSSHFYIPRTGEVIQFVPCEKRAWHAGESCWQGRSRCNDFSIGIELEG
CCCHHHHHHCEEECCEEECCCCCCEEEEECCCCHHCCCCHHHHHHHHCCCCEEEEEEEEC
SDSQPFTDSQYRMLVGLTLELQKAYPIEHIAGHSDIAPGRKTDPGPCFDWKRYRTALAAS
CCCCCCCCCCEEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
R
C
>Mature Secondary Structure
MQIDATGCARGIRFIASPNFDERPGGCAISLLVIHNISLPPDQFGGNGVAELFTNELDPE
CEECCHHHHHHEEEEECCCCCCCCCCEEEEEEEEECCCCCCHHCCCCCHHHHHHCCCCCC
AHPYYRTIHGLRVSSHFYIPRTGEVIQFVPCEKRAWHAGESCWQGRSRCNDFSIGIELEG
CCCHHHHHHCEEECCEEECCCCCCEEEEECCCCHHCCCCHHHHHHHHCCCCEEEEEEEEC
SDSQPFTDSQYRMLVGLTLELQKAYPIEHIAGHSDIAPGRKTDPGPCFDWKRYRTALAAS
CCCCCCCCCCEEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]