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The map label for this gene is tilS [H]

Identifier: 82702932

GI number: 82702932

Start: 2069267

End: 2070685

Strand: Reverse

Name: tilS [H]

Synonym: Nmul_A1809

Alternate gene names: 82702932

Gene position: 2070685-2069267 (Counterclockwise)

Preceding gene: 82702933

Following gene: 82702928

Centisome position: 65.03

GC content: 56.8

Gene sequence:

>1419_bases
ATGGCCAGTTCAAGGAAGTCAAAGTCAGATAATATCTCGCGCAGGGCGCAGCATGTATTGCGTACCCAGGTCCCAGCTGG
CGATCACCTGACCATCGCTTTGAGCGGGGGAGTGGATTCGGTGGTGTTGCTGGATCTGCTTGTCCCGCTTGCCTCGCAAA
TGCAACTCCCGCTCTCGGCCGTTCATGTCAACCACGGTATCAGCCCCAATGCAGATAAATGGAGTGAATTCTGCCGGAAT
CTGTGCCAGTTCCGAAACATTCCTCTTGAAATTGCCAGGGTAAAAGTTGCCCAAGGACCCGGTATCAGCCTGGAAGCCGC
GGCACGTGAAGAGCGCTATCGCATCTTTAGGGGTCTTCAAACGGATTATATCGTGCTGGCCCAGCATCTGGATGACCAGG
CGGAAACGCTGCTGCTGCAATTGCTGCGTGGTGCGGGGGTAAAGGGGCTGAGCGCGATGCCGGTCGTGAGGACCGTGCGA
AAAGAAATGGGTGAGACAGTCCCGGTGAACTCCGTCCCTGCACTTGAGGGCAGACCGAGGCTGCTTCGGCCTCTGCTCGA
CGTTTCGCGACGGGAGATCGAAGACTATGCGAGAAAGCATGCCTTGCGGTGGATTACCGATGAAAGCAACGACGAGATAT
CTTTCGGCCGGAATTTTCTGCGTCACGAACTTTTTCCCCTCATGGAAAAGCGCTTCCCCGCTTACCGTACGACTTTCCTG
CGCGCCAGCCGCCATATGGCTGAGGCTTCCGCTTTACTGGATGAATTGGCCGAAGTCGATAGTGCGCGATGTGCAGTACC
CGGAAAACTCCATGTCGAAGATCTGCGGAAACTGGGTTTTCCGCGTGCGCGGAACCTGTTGCGCTATACGCTGGCGCAGC
ATGCCGTCGTACTGCCCAGCACGGTAAAGCTTGAAGAAATCCTGCGGCAGCTGGTCTCCTCGTATTCCGATACAAAACTG
CATGTCGTTTTCGGGAATACGGAAATCCGGTGCTTCAGAGGCCGGGTACATATACGAAAAGCCCAAACGATAGTCGAAGC
GATTCCCGAGGCCCGCTGGCATTTGCCCTGGCGAGGAGAAGAGCAGCTTTTCATTTCCGAGCTTGGCGTAACCCTTCGAT
TTACACGTTCTATGGGCGCCGGGATCGGCTGGCAAAAGATCAACGGGCAACCCGTAACGATCCGTCTGCGCCAGGGGGGG
GAACGGCTGCGTCCAGACTGCAAGCGCCCACGCCGGAGCCTTAAAAACCTGCTGCGGGAAGCATCGCTCCCACCCTGGCA
ACGGCAAAGGTTACCGCTGATATTCAGCGGAGAGCAGCTGGTCTGCGTACCGGGAATAGGCGTGGATTGCGATTTTCAGG
CAGCGGACGACGAACAGGGTCTCGTGGTGGAGTGGCGGCAGGACCTTTTTTCACCCTGA

Upstream 100 bases:

>100_bases
ATGCAAGCCGTAAAGAAAGCCTTGCAGGACTCGCTCAAGCTCCTGCAGGAATATTCCATCCACATGTTGCTGGAGAAACG
CTTTGAGCGGCTGATGGGAT

Downstream 100 bases:

>100_bases
TTTTTGTTCACCACAATTCAAACACCAGCACTTCCGCTTTTTGACCCTGATCGAGAACCAGACGATCTTCGCCGGTAAGC
ATGGCGGCATCTCCCGTTTC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MASSRKSKSDNISRRAQHVLRTQVPAGDHLTIALSGGVDSVVLLDLLVPLASQMQLPLSAVHVNHGISPNADKWSEFCRN
LCQFRNIPLEIARVKVAQGPGISLEAAAREERYRIFRGLQTDYIVLAQHLDDQAETLLLQLLRGAGVKGLSAMPVVRTVR
KEMGETVPVNSVPALEGRPRLLRPLLDVSRREIEDYARKHALRWITDESNDEISFGRNFLRHELFPLMEKRFPAYRTTFL
RASRHMAEASALLDELAEVDSARCAVPGKLHVEDLRKLGFPRARNLLRYTLAQHAVVLPSTVKLEEILRQLVSSYSDTKL
HVVFGNTEIRCFRGRVHIRKAQTIVEAIPEARWHLPWRGEEQLFISELGVTLRFTRSMGAGIGWQKINGQPVTIRLRQGG
ERLRPDCKRPRRSLKNLLREASLPPWQRQRLPLIFSGEQLVCVPGIGVDCDFQAADDEQGLVVEWRQDLFSP

Sequences:

>Translated_472_residues
MASSRKSKSDNISRRAQHVLRTQVPAGDHLTIALSGGVDSVVLLDLLVPLASQMQLPLSAVHVNHGISPNADKWSEFCRN
LCQFRNIPLEIARVKVAQGPGISLEAAAREERYRIFRGLQTDYIVLAQHLDDQAETLLLQLLRGAGVKGLSAMPVVRTVR
KEMGETVPVNSVPALEGRPRLLRPLLDVSRREIEDYARKHALRWITDESNDEISFGRNFLRHELFPLMEKRFPAYRTTFL
RASRHMAEASALLDELAEVDSARCAVPGKLHVEDLRKLGFPRARNLLRYTLAQHAVVLPSTVKLEEILRQLVSSYSDTKL
HVVFGNTEIRCFRGRVHIRKAQTIVEAIPEARWHLPWRGEEQLFISELGVTLRFTRSMGAGIGWQKINGQPVTIRLRQGG
ERLRPDCKRPRRSLKNLLREASLPPWQRQRLPLIFSGEQLVCVPGIGVDCDFQAADDEQGLVVEWRQDLFSP
>Mature_471_residues
ASSRKSKSDNISRRAQHVLRTQVPAGDHLTIALSGGVDSVVLLDLLVPLASQMQLPLSAVHVNHGISPNADKWSEFCRNL
CQFRNIPLEIARVKVAQGPGISLEAAAREERYRIFRGLQTDYIVLAQHLDDQAETLLLQLLRGAGVKGLSAMPVVRTVRK
EMGETVPVNSVPALEGRPRLLRPLLDVSRREIEDYARKHALRWITDESNDEISFGRNFLRHELFPLMEKRFPAYRTTFLR
ASRHMAEASALLDELAEVDSARCAVPGKLHVEDLRKLGFPRARNLLRYTLAQHAVVLPSTVKLEEILRQLVSSYSDTKLH
VVFGNTEIRCFRGRVHIRKAQTIVEAIPEARWHLPWRGEEQLFISELGVTLRFTRSMGAGIGWQKINGQPVTIRLRQGGE
RLRPDCKRPRRSLKNLLREASLPPWQRQRLPLIFSGEQLVCVPGIGVDCDFQAADDEQGLVVEWRQDLFSP

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=465, Percent_Identity=33.7634408602151, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53433; Mature: 53301

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRKSKSDNISRRAQHVLRTQVPAGDHLTIALSGGVDSVVLLDLLVPLASQMQLPLSA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHH
VHVNHGISPNADKWSEFCRNLCQFRNIPLEIARVKVAQGPGISLEAAAREERYRIFRGLQ
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCC
TDYIVLAQHLDDQAETLLLQLLRGAGVKGLSAMPVVRTVRKEMGETVPVNSVPALEGRPR
CCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
LLRPLLDVSRREIEDYARKHALRWITDESNDEISFGRNFLRHELFPLMEKRFPAYRTTFL
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RASRHMAEASALLDELAEVDSARCAVPGKLHVEDLRKLGFPRARNLLRYTLAQHAVVLPS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCEECCC
TVKLEEILRQLVSSYSDTKLHVVFGNTEIRCFRGRVHIRKAQTIVEAIPEARWHLPWRGE
HHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHCHHHCCCCCCCC
EQLFISELGVTLRFTRSMGAGIGWQKINGQPVTIRLRQGGERLRPDCKRPRRSLKNLLRE
CHHHHHHCCCCEEEEHHHCCCCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ASLPPWQRQRLPLIFSGEQLVCVPGIGVDCDFQAADDEQGLVVEWRQDLFSP
CCCCCHHHCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCEEHHHHHHCCCC
>Mature Secondary Structure 
ASSRKSKSDNISRRAQHVLRTQVPAGDHLTIALSGGVDSVVLLDLLVPLASQMQLPLSA
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHH
VHVNHGISPNADKWSEFCRNLCQFRNIPLEIARVKVAQGPGISLEAAAREERYRIFRGLQ
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCC
TDYIVLAQHLDDQAETLLLQLLRGAGVKGLSAMPVVRTVRKEMGETVPVNSVPALEGRPR
CCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
LLRPLLDVSRREIEDYARKHALRWITDESNDEISFGRNFLRHELFPLMEKRFPAYRTTFL
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RASRHMAEASALLDELAEVDSARCAVPGKLHVEDLRKLGFPRARNLLRYTLAQHAVVLPS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCEECCC
TVKLEEILRQLVSSYSDTKLHVVFGNTEIRCFRGRVHIRKAQTIVEAIPEARWHLPWRGE
HHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHCHHHCCCCCCCC
EQLFISELGVTLRFTRSMGAGIGWQKINGQPVTIRLRQGGERLRPDCKRPRRSLKNLLRE
CHHHHHHCCCCEEEEHHHCCCCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ASLPPWQRQRLPLIFSGEQLVCVPGIGVDCDFQAADDEQGLVVEWRQDLFSP
CCCCCHHHCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]