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The map label for this gene is hypF [H]

Identifier: 82702792

GI number: 82702792

Start: 1905707

End: 1908019

Strand: Direct

Name: hypF [H]

Synonym: Nmul_A1668

Alternate gene names: 82702792

Gene position: 1905707-1908019 (Clockwise)

Preceding gene: 82702791

Following gene: 82702793

Centisome position: 59.85

GC content: 56.51

Gene sequence:

>2313_bases
ATGAATATGAAAGAGGAGCAAATTACTCGACCGTGTAAACCCGAAGCGCGCCGCTGGCTCATAGGGGGGCGGGTGCAGGG
CGTGGGTTTTCGCCCGTTTGTGTACCGTCTGGCGCAGCGATTCCGCTTGACTGGGCATGTGCAGAATACATCCGGCCAGG
TGGTGATCGAAGCAGCGGGAAAAAAAAAGCTTCTGGATGTGTTTGGCGTCGCACTGCTTGCCGAGGCACCCCCTCTTGCT
CAACCGGAGATGGTGCACGTTGAGAAAATCCCCTACCTCGAAAGGGATGGCTTCGAAATCCTGCAAAGCTCTGACAATTC
GTCAGGGCGCATTCACATTCCTCCGGATTATTTCTTGTGCGATGACTGTAAGCGGGAAATGCAAACACCGGGTGACAGGC
GCTATCGCTACCCCTTCATTAACTGCACCCAGTGCGGTCCCCGCTACACGCTGATCACCAAGCTGCCTTACGATCGTCCT
AATACGGTGATGACAAGCTTCGAATTATGCTCTCAATGCCTGAGAGAGTATGAAGATCCGGGCGACAGGCGTTTTCATGC
CGAACCGGTAGCTTGTCCGACATGTGGCCCACAGCTTTGCCTGTCGGCACCCGAAGGCCGGATTAACGGACCCGCCGCGC
TCACAGCTTCTGTTACCGCCCTGCGGCGAGGTGAGATCGTTGCAGTGAAGGGCATTGGCGGTTATCACCTGATGTGTGAT
GCCCGCAACGAGACAGCGATTGGGCAGTTGCGCACAAACAAGCATCGCCCACATAAACCGCTTGCGCTGATGTTTCCCTG
GTGCGGTGATGATGGACTAGCGCAGGTGCGGGATGAATTGGAGATCGATGCTCAAGGGCAAGCATTACTGCGGGCGCCAG
CGCGGCCCATCGTGCTCTTGCGAAGGCGCAGAGATTCAATGTTGCCTCGATCTATCACCCCCGGGATAAGGGAAATCGGT
GTAATGCTCCCCTATAGTCCCCTGCATCATTTGTTGCTCCATGAACTGGATAAACCGGTAGTGGCCACCTCGGGAAATGT
GAGCGGAGAGCCAGTACTGACCGCTAATGCAGAGGCGGAAACGCGGTTGGCCCGAGTGACTCGGGTTTTTCTTCATCATA
ATCGCCCCATTGCGCGTCCCGCGGATGATCCGGTGGTCCGTGTCATTGCAGGAAAGCCTCGTTTGGTTCGAGTGGGACGC
GGCGTTGGCCCGGTTGAATTCGACCTGCCTGGCCTGTTTGTGCAGCCATTGCTGGCTGTGGGCGGGCATATGAAAAACAG
CATCGCCTTGGGCTGGAATGGGCGGGCCATCCTTTCTCCCCATATCGGGGACCTGGATGCTCCCCGAAGCATGGCAGTAT
TCGAACAGGTCATTTCGGATTTGCAGCGATTGTATGGCGCTTTCCCGCAGGCTATTGTTTGCGATGCCCATAAAGGGTAT
GCCAGCAGCCGCTGGGCGGATCGGCAGGGCTTGCCACTCATTCCCGTCTGGCATCATCATGCCCATGCATCTGCTTTGGC
GGCTGAACATTCGCGAGAACTTACATGGTTGACTTTCACCTGGGATGGCGTCGGCTTGGGGGAAGACAATACCCTTTGGG
GAGGCGAAGCATTGCTTGGTCGGCCAGGAGTCTGGCGCCGAGTCGCCCGCCTGTGTCCTTTCCGGCTACCAGGAGGCGAG
CGGGCAGGCCGGGAACCGTGGCGTTCTGCTGCTGCCCTTTGCTGGGAAAGCGGAATAGAGCCTCCTGCCATGGGAATCCG
CCTGGAACTGCTAAAAAAAGCCTGGAAAAAAGGACTGAATTCGCGCGTCACCACTGCAGGGGGACGTCTTTTTGACGGCG
CTGCCAGCCTGCTCGGGTTACTCAGACATGCGAGTTACGAGGGCCAGGCGGGGATGTATCTGGAAAATATTGCAGACGCC
TGCGATTCAGAAAGCGAGGTGGATGTAATTGATCTGCCTCTTTTCCAAAGTGCTACCGGTTTGCTCACCGCGGACTGGAA
ACCGTTGCTCCCCGCGCTGCTCAATGAGGCGATTCCGGTTGCGCGAAGGGCAATGCAATTTCACTTAAGTCTTGCAAATT
CCATTATGGTCCAAGCGCAACGTATTCATGCATCAACACCGTTTGATCGGGTAGGCCTGACCGGCGGGGTTTTCCAGAAC
AAACTGCTTGCCGAGTTGGCTGTTTCACAACTGACAGCTGCCGGGTTTGAAGCATATTTGCCGGAAAGAACCCCTTGCAA
TGATGGAGGCATTGCGCTGGGTCAATTGGTCGAAGCAGCTGCGGGCCTCGAAGCAGGTGCGCATGGAGGATAG

Upstream 100 bases:

>100_bases
GAGAAGCCAGAATAAGCGGCGCAGGTTTACGGCAAGAGGCTTGGAAGCCGGACGCATTATATCCTGTTCGGTTCTTTTCT
CTCATTTCTCCTGCTGCCCT

Downstream 100 bases:

>100_bases
GCACGTCAGCTTGGCCCATGGCAATGGCGGCAGTTACATGCGGGAGCTGATCGAAACCGTGTTTGCGCGCCATCTTTCCA
ACCCCTTGCTCGATGTACAG

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MNMKEEQITRPCKPEARRWLIGGRVQGVGFRPFVYRLAQRFRLTGHVQNTSGQVVIEAAGKKKLLDVFGVALLAEAPPLA
QPEMVHVEKIPYLERDGFEILQSSDNSSGRIHIPPDYFLCDDCKREMQTPGDRRYRYPFINCTQCGPRYTLITKLPYDRP
NTVMTSFELCSQCLREYEDPGDRRFHAEPVACPTCGPQLCLSAPEGRINGPAALTASVTALRRGEIVAVKGIGGYHLMCD
ARNETAIGQLRTNKHRPHKPLALMFPWCGDDGLAQVRDELEIDAQGQALLRAPARPIVLLRRRRDSMLPRSITPGIREIG
VMLPYSPLHHLLLHELDKPVVATSGNVSGEPVLTANAEAETRLARVTRVFLHHNRPIARPADDPVVRVIAGKPRLVRVGR
GVGPVEFDLPGLFVQPLLAVGGHMKNSIALGWNGRAILSPHIGDLDAPRSMAVFEQVISDLQRLYGAFPQAIVCDAHKGY
ASSRWADRQGLPLIPVWHHHAHASALAAEHSRELTWLTFTWDGVGLGEDNTLWGGEALLGRPGVWRRVARLCPFRLPGGE
RAGREPWRSAAALCWESGIEPPAMGIRLELLKKAWKKGLNSRVTTAGGRLFDGAASLLGLLRHASYEGQAGMYLENIADA
CDSESEVDVIDLPLFQSATGLLTADWKPLLPALLNEAIPVARRAMQFHLSLANSIMVQAQRIHASTPFDRVGLTGGVFQN
KLLAELAVSQLTAAGFEAYLPERTPCNDGGIALGQLVEAAAGLEAGAHGG

Sequences:

>Translated_770_residues
MNMKEEQITRPCKPEARRWLIGGRVQGVGFRPFVYRLAQRFRLTGHVQNTSGQVVIEAAGKKKLLDVFGVALLAEAPPLA
QPEMVHVEKIPYLERDGFEILQSSDNSSGRIHIPPDYFLCDDCKREMQTPGDRRYRYPFINCTQCGPRYTLITKLPYDRP
NTVMTSFELCSQCLREYEDPGDRRFHAEPVACPTCGPQLCLSAPEGRINGPAALTASVTALRRGEIVAVKGIGGYHLMCD
ARNETAIGQLRTNKHRPHKPLALMFPWCGDDGLAQVRDELEIDAQGQALLRAPARPIVLLRRRRDSMLPRSITPGIREIG
VMLPYSPLHHLLLHELDKPVVATSGNVSGEPVLTANAEAETRLARVTRVFLHHNRPIARPADDPVVRVIAGKPRLVRVGR
GVGPVEFDLPGLFVQPLLAVGGHMKNSIALGWNGRAILSPHIGDLDAPRSMAVFEQVISDLQRLYGAFPQAIVCDAHKGY
ASSRWADRQGLPLIPVWHHHAHASALAAEHSRELTWLTFTWDGVGLGEDNTLWGGEALLGRPGVWRRVARLCPFRLPGGE
RAGREPWRSAAALCWESGIEPPAMGIRLELLKKAWKKGLNSRVTTAGGRLFDGAASLLGLLRHASYEGQAGMYLENIADA
CDSESEVDVIDLPLFQSATGLLTADWKPLLPALLNEAIPVARRAMQFHLSLANSIMVQAQRIHASTPFDRVGLTGGVFQN
KLLAELAVSQLTAAGFEAYLPERTPCNDGGIALGQLVEAAAGLEAGAHGG
>Mature_770_residues
MNMKEEQITRPCKPEARRWLIGGRVQGVGFRPFVYRLAQRFRLTGHVQNTSGQVVIEAAGKKKLLDVFGVALLAEAPPLA
QPEMVHVEKIPYLERDGFEILQSSDNSSGRIHIPPDYFLCDDCKREMQTPGDRRYRYPFINCTQCGPRYTLITKLPYDRP
NTVMTSFELCSQCLREYEDPGDRRFHAEPVACPTCGPQLCLSAPEGRINGPAALTASVTALRRGEIVAVKGIGGYHLMCD
ARNETAIGQLRTNKHRPHKPLALMFPWCGDDGLAQVRDELEIDAQGQALLRAPARPIVLLRRRRDSMLPRSITPGIREIG
VMLPYSPLHHLLLHELDKPVVATSGNVSGEPVLTANAEAETRLARVTRVFLHHNRPIARPADDPVVRVIAGKPRLVRVGR
GVGPVEFDLPGLFVQPLLAVGGHMKNSIALGWNGRAILSPHIGDLDAPRSMAVFEQVISDLQRLYGAFPQAIVCDAHKGY
ASSRWADRQGLPLIPVWHHHAHASALAAEHSRELTWLTFTWDGVGLGEDNTLWGGEALLGRPGVWRRVARLCPFRLPGGE
RAGREPWRSAAALCWESGIEPPAMGIRLELLKKAWKKGLNSRVTTAGGRLFDGAASLLGLLRHASYEGQAGMYLENIADA
CDSESEVDVIDLPLFQSATGLLTADWKPLLPALLNEAIPVARRAMQFHLSLANSIMVQAQRIHASTPFDRVGLTGGVFQN
KLLAELAVSQLTAAGFEAYLPERTPCNDGGIALGQLVEAAAGLEAGAHGG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=759, Percent_Identity=40.4479578392622, Blast_Score=431, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 84352; Mature: 84352

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKEEQITRPCKPEARRWLIGGRVQGVGFRPFVYRLAQRFRLTGHVQNTSGQVVIEAAG
CCCCHHHHCCCCCHHHHEEEECCEECCCCCHHHHHHHHHHHHHEEEEECCCCCEEEEECC
KKKLLDVFGVALLAEAPPLAQPEMVHVEKIPYLERDGFEILQSSDNSSGRIHIPPDYFLC
CHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCCCCCCCHHHHHCCCCCCCEEECCCCCEEC
DDCKREMQTPGDRRYRYPFINCTQCGPRYTLITKLPYDRPNTVMTSFELCSQCLREYEDP
HHHHHHHCCCCCCEEECCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
GDRRFHAEPVACPTCGPQLCLSAPEGRINGPAALTASVTALRRGEIVAVKGIGGYHLMCD
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEE
ARNETAIGQLRTNKHRPHKPLALMFPWCGDDGLAQVRDELEIDAQGQALLRAPARPIVLL
CCCCCEEHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHCCCCHHHHH
RRRRDSMLPRSITPGIREIGVMLPYSPLHHLLLHELDKPVVATSGNVSGEPVLTANAEAE
HHHHHHCCCCCCCCCHHHHCEECCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHH
TRLARVTRVFLHHNRPIARPADDPVVRVIAGKPRLVRVGRGVGPVEFDLPGLFVQPLLAV
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCEECCCCHHHHHHHHH
GGHMKNSIALGWNGRAILSPHIGDLDAPRSMAVFEQVISDLQRLYGAFPQAIVCDAHKGY
CCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
ASSRWADRQGLPLIPVWHHHAHASALAAEHSRELTWLTFTWDGVGLGEDNTLWGGEALLG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEECCCHHCC
RPGVWRRVARLCPFRLPGGERAGREPWRSAAALCWESGIEPPAMGIRLELLKKAWKKGLN
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
SRVTTAGGRLFDGAASLLGLLRHASYEGQAGMYLENIADACDSESEVDVIDLPLFQSATG
CCEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHCCCC
LLTADWKPLLPALLNEAIPVARRAMQFHLSLANSIMVQAQRIHASTPFDRVGLTGGVFQN
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHH
KLLAELAVSQLTAAGFEAYLPERTPCNDGGIALGQLVEAAAGLEAGAHGG
HHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNMKEEQITRPCKPEARRWLIGGRVQGVGFRPFVYRLAQRFRLTGHVQNTSGQVVIEAAG
CCCCHHHHCCCCCHHHHEEEECCEECCCCCHHHHHHHHHHHHHEEEEECCCCCEEEEECC
KKKLLDVFGVALLAEAPPLAQPEMVHVEKIPYLERDGFEILQSSDNSSGRIHIPPDYFLC
CHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCCCCCCCHHHHHCCCCCCCEEECCCCCEEC
DDCKREMQTPGDRRYRYPFINCTQCGPRYTLITKLPYDRPNTVMTSFELCSQCLREYEDP
HHHHHHHCCCCCCEEECCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
GDRRFHAEPVACPTCGPQLCLSAPEGRINGPAALTASVTALRRGEIVAVKGIGGYHLMCD
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEE
ARNETAIGQLRTNKHRPHKPLALMFPWCGDDGLAQVRDELEIDAQGQALLRAPARPIVLL
CCCCCEEHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHCCCCHHHHH
RRRRDSMLPRSITPGIREIGVMLPYSPLHHLLLHELDKPVVATSGNVSGEPVLTANAEAE
HHHHHHCCCCCCCCCHHHHCEECCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHH
TRLARVTRVFLHHNRPIARPADDPVVRVIAGKPRLVRVGRGVGPVEFDLPGLFVQPLLAV
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCEECCCCHHHHHHHHH
GGHMKNSIALGWNGRAILSPHIGDLDAPRSMAVFEQVISDLQRLYGAFPQAIVCDAHKGY
CCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
ASSRWADRQGLPLIPVWHHHAHASALAAEHSRELTWLTFTWDGVGLGEDNTLWGGEALLG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEECCCHHCC
RPGVWRRVARLCPFRLPGGERAGREPWRSAAALCWESGIEPPAMGIRLELLKKAWKKGLN
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
SRVTTAGGRLFDGAASLLGLLRHASYEGQAGMYLENIADACDSESEVDVIDLPLFQSATG
CCEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEECCCHHCCCC
LLTADWKPLLPALLNEAIPVARRAMQFHLSLANSIMVQAQRIHASTPFDRVGLTGGVFQN
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHH
KLLAELAVSQLTAAGFEAYLPERTPCNDGGIALGQLVEAAAGLEAGAHGG
HHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]