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The map label for this gene is pleD [H]

Identifier: 82702630

GI number: 82702630

Start: 1716741

End: 1717823

Strand: Direct

Name: pleD [H]

Synonym: Nmul_A1501

Alternate gene names: 82702630

Gene position: 1716741-1717823 (Clockwise)

Preceding gene: 82702628

Following gene: 82702640

Centisome position: 53.91

GC content: 50.14

Gene sequence:

>1083_bases
GTGAATACCGAAGCAACGCCCCATTGTGCAGCACTCGGGTTCGACAAAGACCGAATCCGGCAGGCACTTTCCTTGCTGGG
ACTGGCTGCGGATCACAAAGAAGAAGCGGCGGCAATCAACCTGCTCACCGAATTCATCGCTGAAAGAGTCGATAAAATAA
TCAATTCCTGCCTTGCCGTAATTGCGGGCAGCGATGACTTTGAATTGATCGAGAGGAACATCGGTACCGAAGCCTTCAGG
CAGAAATGGAAATATCGCTTGCGATCCTTTGGTAAAAATTTTGCTACTCCAGGTTATTTTGAAGAGCGGCTGGCGCTGGC
TGCTGCTTTCGCGCATGCAAATATCCCTTTGGGAGCCTTGCAGCTTCCTTCTTTTCTCATACAGCAGTCCTTGATAGACA
ATCTGTTGGACAAGTCCGATTTTAATGCCGATAAAACCAGAATACTCCTATGCACTATCCTGAAACTCACTGCACTTGAC
TTGTACATTGCCGCCGAAGGTTATCACCTTCCGGAACTCAATGAGCTGGAAGAAAAGCTGGACAAGCTGCGGGCGGAAGC
ATCGCGCTTGCAGGAAAGAACCTGGACCGACGAGCTGACAGGTGTAATGAACTACAGAAAGCTCATGGAGTCTCTCGAAC
ACCATGTCCAACGAGCGGAGCGTAGAGGCCAAGAACGCAGCTCTTTGTGCCTCATCATGTCGGATCTCGATTTCTTCAAG
AAAATCAACGACACCTACGGCCATATGGTCGGCGATCTGGTGCTACAGCATTTGGCGAGGCGTATAAAATCCGCAACACG
CGACTTCGACATGATCGGCCGGTTCGGCGGGGAGGAGTTCGTCGTTATTATGGCGGACACCGATCTCGAATTGGCAAAGG
TAATTGCCGAGCGCATACGCCTGGGAGTCATGAGCACGCCATTTCATATAAAAAAATTTAACATCCACGTTACCATCAGC
TTGGGCGTAGCAATGCTTAAACCTGGGGAATCCAGAGAATCACTGCTTGAGCGGGCAGATGCGGCAATGTATGAGGCGAA
AAGAAGAGGCCGGAATTGCGTGGTGACTACCAAGGAAATGTAG

Upstream 100 bases:

>100_bases
GACCAATAGTAAGAATAGCAAGCAGGGAGAATAATGCGTTATCCTTTATGCTGCAACCCGCCCCACCTCAGGTTTCCTAC
ACATCAATCGTCGCGAATAA

Downstream 100 bases:

>100_bases
GGGTCCAACAATCATCCGTTCATATTACTGTCCGAACAACACGCGCCCCTCTGATACCCAGGCAATAGCACAGCGAGAAG
TGAGGGACGATGGGGCTACA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MNTEATPHCAALGFDKDRIRQALSLLGLAADHKEEAAAINLLTEFIAERVDKIINSCLAVIAGSDDFELIERNIGTEAFR
QKWKYRLRSFGKNFATPGYFEERLALAAAFAHANIPLGALQLPSFLIQQSLIDNLLDKSDFNADKTRILLCTILKLTALD
LYIAAEGYHLPELNELEEKLDKLRAEASRLQERTWTDELTGVMNYRKLMESLEHHVQRAERRGQERSSLCLIMSDLDFFK
KINDTYGHMVGDLVLQHLARRIKSATRDFDMIGRFGGEEFVVIMADTDLELAKVIAERIRLGVMSTPFHIKKFNIHVTIS
LGVAMLKPGESRESLLERADAAMYEAKRRGRNCVVTTKEM

Sequences:

>Translated_360_residues
MNTEATPHCAALGFDKDRIRQALSLLGLAADHKEEAAAINLLTEFIAERVDKIINSCLAVIAGSDDFELIERNIGTEAFR
QKWKYRLRSFGKNFATPGYFEERLALAAAFAHANIPLGALQLPSFLIQQSLIDNLLDKSDFNADKTRILLCTILKLTALD
LYIAAEGYHLPELNELEEKLDKLRAEASRLQERTWTDELTGVMNYRKLMESLEHHVQRAERRGQERSSLCLIMSDLDFFK
KINDTYGHMVGDLVLQHLARRIKSATRDFDMIGRFGGEEFVVIMADTDLELAKVIAERIRLGVMSTPFHIKKFNIHVTIS
LGVAMLKPGESRESLLERADAAMYEAKRRGRNCVVTTKEM
>Mature_360_residues
MNTEATPHCAALGFDKDRIRQALSLLGLAADHKEEAAAINLLTEFIAERVDKIINSCLAVIAGSDDFELIERNIGTEAFR
QKWKYRLRSFGKNFATPGYFEERLALAAAFAHANIPLGALQLPSFLIQQSLIDNLLDKSDFNADKTRILLCTILKLTALD
LYIAAEGYHLPELNELEEKLDKLRAEASRLQERTWTDELTGVMNYRKLMESLEHHVQRAERRGQERSSLCLIMSDLDFFK
KINDTYGHMVGDLVLQHLARRIKSATRDFDMIGRFGGEEFVVIMADTDLELAKVIAERIRLGVMSTPFHIKKFNIHVTIS
LGVAMLKPGESRESLLERADAAMYEAKRRGRNCVVTTKEM

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=39.375, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=37.8531073446328, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=38.9221556886228, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=38.0681818181818, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=35.2201257861635, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787541, Length=181, Percent_Identity=33.1491712707182, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082007, Length=173, Percent_Identity=38.150289017341, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787802, Length=166, Percent_Identity=33.7349397590361, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081977, Length=174, Percent_Identity=30.4597701149425, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=153, Percent_Identity=32.6797385620915, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.3170731707317, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=30.7262569832402, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=183, Percent_Identity=30.6010928961749, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=32.9268292682927, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40789; Mature: 40789

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEATPHCAALGFDKDRIRQALSLLGLAADHKEEAAAINLLTEFIAERVDKIINSCLAV
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGSDDFELIERNIGTEAFRQKWKYRLRSFGKNFATPGYFEERLALAAAFAHANIPLGAL
HCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
QLPSFLIQQSLIDNLLDKSDFNADKTRILLCTILKLTALDLYIAAEGYHLPELNELEEKL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHH
DKLRAEASRLQERTWTDELTGVMNYRKLMESLEHHVQRAERRGQERSSLCLIMSDLDFFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEHHHHHHHHH
KINDTYGHMVGDLVLQHLARRIKSATRDFDMIGRFGGEEFVVIMADTDLELAKVIAERIR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
LGVMSTPFHIKKFNIHVTISLGVAMLKPGESRESLLERADAAMYEAKRRGRNCVVTTKEM
HCCCCCCCEEEEEEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MNTEATPHCAALGFDKDRIRQALSLLGLAADHKEEAAAINLLTEFIAERVDKIINSCLAV
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGSDDFELIERNIGTEAFRQKWKYRLRSFGKNFATPGYFEERLALAAAFAHANIPLGAL
HCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
QLPSFLIQQSLIDNLLDKSDFNADKTRILLCTILKLTALDLYIAAEGYHLPELNELEEKL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHH
DKLRAEASRLQERTWTDELTGVMNYRKLMESLEHHVQRAERRGQERSSLCLIMSDLDFFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEHHHHHHHHH
KINDTYGHMVGDLVLQHLARRIKSATRDFDMIGRFGGEEFVVIMADTDLELAKVIAERIR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
LGVMSTPFHIKKFNIHVTISLGVAMLKPGESRESLLERADAAMYEAKRRGRNCVVTTKEM
HCCCCCCCEEEEEEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA