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The map label for this gene is bioD

Identifier: 82702262

GI number: 82702262

Start: 1302418

End: 1303098

Strand: Direct

Name: bioD

Synonym: Nmul_A1133

Alternate gene names: 82702262

Gene position: 1302418-1303098 (Clockwise)

Preceding gene: 82702261

Following gene: 82702265

Centisome position: 40.9

GC content: 55.51

Gene sequence:

>681_bases
ATGGGAATGGGAAAGGGGTATTTCGTCACCGGGACAGATACCGGCGTGGGTAAAACGCGCGTGACCTGCGCCCTGCTGCA
TGCCTTTGCTGCCACCGGAAAAACTGTGGTGGGCATGAAGCCTGTCGCAGCCGGATGCGAAAACGGGATGTGGCCTGATG
TCGAATTACTGGCTGCTGCAAGCAACATTTCCGTGCAACGCGAGCATATCAATCCTTATGCGCTGGTTCCTCCAATTGCT
CCGCACATTGCTGCAGACAGGGCAGGCATTGAAATCGATCTCGAAGTCATTCGCCAGGCACACCTTGAGCTGAAAAAAAA
AGCGGATATTGTGATCGTGGAAGGAGCAGGAGGATTTCTCGTCCCCTTGAACGATCATGAGGACAGCGTAGCCCTGGTGC
AGGCTTTGGGTCTAGCGGTCCTGCTGGTGGTGGGGATGCGGCTCGGCTGTATCAACCACGCACTGTTGACGGCCCATGCC
GTTCGAGCAGCCCAAATTCCGCTGGCGGGATGGGTGGCAAACCGGATCGATCCGGAAATGGCGGTTTTCAAGGAGAACGT
ACTGGCCCTGGAGCAGCGGCTTGACTGTCCACTGCTGGGTATATTGCCTTACGATCAGAACCATGATGCCAGGGATTTGT
CGTCCTTGCTGGATATTGCAAGAATCGGGATGAGCTCCTAA

Upstream 100 bases:

>100_bases
GCGACTTTTGAAGTGGTGTATGGCCATGCCTGGAAACCGGCGCCCCGAACATCCATTCTTACACCGGAAACACGCCGCCA
CATCGGCCTGGAATAAGGAT

Downstream 100 bases:

>100_bases
TAGAAGTGCGTTCCGCGAAGCTCTGCTGAGGGGCATACCCAAGGCATTACCATTATGCCTTGTGCCCCTGCCTGGCCCAA
GCGGAAATCCTGGGAGCAGA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MGMGKGYFVTGTDTGVGKTRVTCALLHAFAATGKTVVGMKPVAAGCENGMWPDVELLAAASNISVQREHINPYALVPPIA
PHIAADRAGIEIDLEVIRQAHLELKKKADIVIVEGAGGFLVPLNDHEDSVALVQALGLAVLLVVGMRLGCINHALLTAHA
VRAAQIPLAGWVANRIDPEMAVFKENVLALEQRLDCPLLGILPYDQNHDARDLSSLLDIARIGMSS

Sequences:

>Translated_226_residues
MGMGKGYFVTGTDTGVGKTRVTCALLHAFAATGKTVVGMKPVAAGCENGMWPDVELLAAASNISVQREHINPYALVPPIA
PHIAADRAGIEIDLEVIRQAHLELKKKADIVIVEGAGGFLVPLNDHEDSVALVQALGLAVLLVVGMRLGCINHALLTAHA
VRAAQIPLAGWVANRIDPEMAVFKENVLALEQRLDCPLLGILPYDQNHDARDLSSLLDIARIGMSS
>Mature_225_residues
GMGKGYFVTGTDTGVGKTRVTCALLHAFAATGKTVVGMKPVAAGCENGMWPDVELLAAASNISVQREHINPYALVPPIAP
HIAADRAGIEIDLEVIRQAHLELKKKADIVIVEGAGGFLVPLNDHEDSVALVQALGLAVLLVVGMRLGCINHALLTAHAV
RAAQIPLAGWVANRIDPEMAVFKENVLALEQRLDCPLLGILPYDQNHDARDLSSLLDIARIGMSS

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=207, Percent_Identity=43.4782608695652, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI87081944, Length=222, Percent_Identity=41.4414414414414, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_NITMU (Q2Y9Y5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411828.1
- ProteinModelPortal:   Q2Y9Y5
- SMR:   Q2Y9Y5
- STRING:   Q2Y9Y5
- GeneID:   3784246
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1133
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   INPGLSH
- PhylomeDB:   Q2Y9Y5
- ProtClustDB:   CLSK586185
- BioCyc:   NMUL323848:NMUL_A1133-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 23960; Mature: 23829

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMGKGYFVTGTDTGVGKTRVTCALLHAFAATGKTVVGMKPVAAGCENGMWPDVELLAAA
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCHHHHHHH
SNISVQREHINPYALVPPIAPHIAADRAGIEIDLEVIRQAHLELKKKADIVIVEGAGGFL
CCCEEECCCCCCEEECCCCCCHHHCCCCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCEE
VPLNDHEDSVALVQALGLAVLLVVGMRLGCINHALLTAHAVRAAQIPLAGWVANRIDPEM
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHH
AVFKENVLALEQRLDCPLLGILPYDQNHDARDLSSLLDIARIGMSS
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GMGKGYFVTGTDTGVGKTRVTCALLHAFAATGKTVVGMKPVAAGCENGMWPDVELLAAA
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCHHHHHHH
SNISVQREHINPYALVPPIAPHIAADRAGIEIDLEVIRQAHLELKKKADIVIVEGAGGFL
CCCEEECCCCCCEEECCCCCCHHHCCCCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCEE
VPLNDHEDSVALVQALGLAVLLVVGMRLGCINHALLTAHAVRAAQIPLAGWVANRIDPEM
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHH
AVFKENVLALEQRLDCPLLGILPYDQNHDARDLSSLLDIARIGMSS
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA