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The map label for this gene is bioF

Identifier: 82702259

GI number: 82702259

Start: 1299418

End: 1300608

Strand: Direct

Name: bioF

Synonym: Nmul_A1130

Alternate gene names: 82702259

Gene position: 1299418-1300608 (Clockwise)

Preceding gene: 82702258

Following gene: 82702260

Centisome position: 40.81

GC content: 56.51

Gene sequence:

>1191_bases
ATGCTCAACGACTTGGATTCAATTCATCAAACGGATGAAGGGCTGCGCGAATACCTGCGCAGCCGTGGAAACCGGAGCTT
GTATCGGTATCGCCTGACACTTGAGAGTCCTCAGGCCCCTCGGATAACGGTAAGCGGTCGTGAATACTTGGCATTTTGCA
GTAATGATTATCTCGGTCTTGCCAATCATCCCGAACTGATTGCAGCGCTTTGCGAAGGCGCGACGCAGTACGGCGTGGGA
GCGGGTACGTCGCATCTCGTCAGCGGACATTCGCGGGCACACCATCTGCTGGAGGAAGCGCTGGCATCCTTTACCCGATT
CCCCCGGGCATTGCTATTTTCCACAGGATACATGGCAAACGCAGGCGTAGTAACAGCGTTGACCGGCCGGGGTGATGCCG
TATTCGGGGACAAACTCAATCATGCCTCCCTCAACGATGCAGCGCTTCTATCGCGCGCGAGGTTGAGTCGTTATCCCCAT
CTGGATCTGGCGACCCTGGAGCGGCAGCTTGCCGCATCTCCAGCCAGACGCAAACTCGTGATATCGGATGCGGTATTCAG
CATGGATGGAGATATCGCACCGCTTCCAGAGTTGCTGGAGTTGTGTGAAAGATACGACGCCTGGTTGCTGCTCGACGATG
CGCACGGATTCGGAGTATTGGGCAATCAGGGGAGGGGGAGTCTGGCGCACTTCAATATCAGTTCGCCGCGCATTATCTAC
ATGGGGACACTCGGCAAAGCGGCGGGTGTATTTGGGGCATTCGTAGCTGCTCAGGAAGAAATCATCGAAACGCTCATCCA
GTGCGCGCGAAGCTACATCTATACTACTGCGACTCCACCCTTCCTGTCTCATGCCTTGCTGAAAAGCCTGGAGCTGATTG
CAGGCGGTGCAGGGCGACGTGAAAAACTGGCGCAACTCACCAAGCTGCTCAAGCAGGAGTGTCATCCGCTGCGGTGGCAA
CTGCTGCCTTCGGATACTCCCATCCAGCCGCTGGTCATGGGAGAGAATGCCGAAGCTCTGCAGGTCAGCGAAGCATTGCG
CCAGAAAGGCATTCTGGTAACCGCCATTCGACCGCCTACCGTGCCCGAGGGGACAGCGCGCCTGCGTATCTCGTTATCCA
GTTCCCATGATATCGAGGATGTTATGGAACTGGGAGCGGCACTGCGGGAAATCGACCGGGATATGGAATGA

Upstream 100 bases:

>100_bases
ACAGGCTGGGGCTGCATTCCATATAGCAGCTTTTCACCTCGATCTTCTTTATTTTCCACTACCCTGCGGCAAATGATCTT
ATCGGGCAACCTCTGCTCGT

Downstream 100 bases:

>100_bases
CTATGGAATGACTGGGCACCTTTACGCCGGATATGACATTCGCCTTGTAAGGGCGACCTCGCGGATACAATGTGGAATGC
TCGATGGGCCAGGTAAGCCT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLNDLDSIHQTDEGLREYLRSRGNRSLYRYRLTLESPQAPRITVSGREYLAFCSNDYLGLANHPELIAALCEGATQYGVG
AGTSHLVSGHSRAHHLLEEALASFTRFPRALLFSTGYMANAGVVTALTGRGDAVFGDKLNHASLNDAALLSRARLSRYPH
LDLATLERQLAASPARRKLVISDAVFSMDGDIAPLPELLELCERYDAWLLLDDAHGFGVLGNQGRGSLAHFNISSPRIIY
MGTLGKAAGVFGAFVAAQEEIIETLIQCARSYIYTTATPPFLSHALLKSLELIAGGAGRREKLAQLTKLLKQECHPLRWQ
LLPSDTPIQPLVMGENAEALQVSEALRQKGILVTAIRPPTVPEGTARLRISLSSSHDIEDVMELGAALREIDRDME

Sequences:

>Translated_396_residues
MLNDLDSIHQTDEGLREYLRSRGNRSLYRYRLTLESPQAPRITVSGREYLAFCSNDYLGLANHPELIAALCEGATQYGVG
AGTSHLVSGHSRAHHLLEEALASFTRFPRALLFSTGYMANAGVVTALTGRGDAVFGDKLNHASLNDAALLSRARLSRYPH
LDLATLERQLAASPARRKLVISDAVFSMDGDIAPLPELLELCERYDAWLLLDDAHGFGVLGNQGRGSLAHFNISSPRIIY
MGTLGKAAGVFGAFVAAQEEIIETLIQCARSYIYTTATPPFLSHALLKSLELIAGGAGRREKLAQLTKLLKQECHPLRWQ
LLPSDTPIQPLVMGENAEALQVSEALRQKGILVTAIRPPTVPEGTARLRISLSSSHDIEDVMELGAALREIDRDME
>Mature_396_residues
MLNDLDSIHQTDEGLREYLRSRGNRSLYRYRLTLESPQAPRITVSGREYLAFCSNDYLGLANHPELIAALCEGATQYGVG
AGTSHLVSGHSRAHHLLEEALASFTRFPRALLFSTGYMANAGVVTALTGRGDAVFGDKLNHASLNDAALLSRARLSRYPH
LDLATLERQLAASPARRKLVISDAVFSMDGDIAPLPELLELCERYDAWLLLDDAHGFGVLGNQGRGSLAHFNISSPRIIY
MGTLGKAAGVFGAFVAAQEEIIETLIQCARSYIYTTATPPFLSHALLKSLELIAGGAGRREKLAQLTKLLKQECHPLRWQ
LLPSDTPIQPLVMGENAEALQVSEALRQKGILVTAIRPPTVPEGTARLRISLSSSHDIEDVMELGAALREIDRDME

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=385, Percent_Identity=34.5454545454545, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI284448556, Length=346, Percent_Identity=35.2601156069364, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI83977442, Length=350, Percent_Identity=32.8571428571429, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI83977444, Length=350, Percent_Identity=32.8571428571429, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI83977440, Length=350, Percent_Identity=32.8571428571429, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4502025, Length=384, Percent_Identity=30.46875, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI40316939, Length=384, Percent_Identity=30.46875, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4758668, Length=358, Percent_Identity=29.0502793296089, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI119220554, Length=364, Percent_Identity=26.9230769230769, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI5454084, Length=291, Percent_Identity=28.5223367697594, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786993, Length=340, Percent_Identity=45.8823529411765, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1790046, Length=374, Percent_Identity=35.0267379679144, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71994529, Length=388, Percent_Identity=30.9278350515464, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI32566772, Length=382, Percent_Identity=26.1780104712042, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17560914, Length=351, Percent_Identity=25.3561253561254, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560912, Length=351, Percent_Identity=25.3561253561254, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982625, Length=277, Percent_Identity=23.826714801444, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71982617, Length=277, Percent_Identity=23.826714801444, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320438, Length=405, Percent_Identity=31.358024691358, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6320267, Length=361, Percent_Identity=27.9778393351801, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323954, Length=298, Percent_Identity=27.5167785234899, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24662918, Length=372, Percent_Identity=33.8709677419355, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17137420, Length=352, Percent_Identity=30.3977272727273, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24653280, Length=321, Percent_Identity=27.4143302180685, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653276, Length=321, Percent_Identity=27.4143302180685, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653278, Length=321, Percent_Identity=27.4143302180685, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17136286, Length=382, Percent_Identity=27.4869109947644, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_NITMU (Q2Y9Y8)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411825.1
- HSSP:   P12998
- ProteinModelPortal:   Q2Y9Y8
- STRING:   Q2Y9Y8
- GeneID:   3784243
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1130
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   NISSPRI
- PhylomeDB:   Q2Y9Y8
- ProtClustDB:   CLSK586188
- BioCyc:   NMUL323848:NMUL_A1130-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 43262; Mature: 43262

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 29-29 BINDING 141-141 BINDING 187-187 BINDING 360-360

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDLDSIHQTDEGLREYLRSRGNRSLYRYRLTLESPQAPRITVSGREYLAFCSNDYLGL
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEECCCCCCC
ANHPELIAALCEGATQYGVGAGTSHLVSGHSRAHHLLEEALASFTRFPRALLFSTGYMAN
CCCHHHHHHHHHCCHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
AGVVTALTGRGDAVFGDKLNHASLNDAALLSRARLSRYPHLDLATLERQLAASPARRKLV
CCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHCEEE
ISDAVFSMDGDIAPLPELLELCERYDAWLLLDDAHGFGVLGNQGRGSLAHFNISSPRIIY
EEHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCEEEEECCCCEEEE
MGTLGKAAGVFGAFVAAQEEIIETLIQCARSYIYTTATPPFLSHALLKSLELIAGGAGRR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCH
EKLAQLTKLLKQECHPLRWQLLPSDTPIQPLVMGENAEALQVSEALRQKGILVTAIRPPT
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCEEEEEECCCC
VPEGTARLRISLSSSHDIEDVMELGAALREIDRDME
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNDLDSIHQTDEGLREYLRSRGNRSLYRYRLTLESPQAPRITVSGREYLAFCSNDYLGL
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEECCCCCCC
ANHPELIAALCEGATQYGVGAGTSHLVSGHSRAHHLLEEALASFTRFPRALLFSTGYMAN
CCCHHHHHHHHHCCHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
AGVVTALTGRGDAVFGDKLNHASLNDAALLSRARLSRYPHLDLATLERQLAASPARRKLV
CCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHCEEE
ISDAVFSMDGDIAPLPELLELCERYDAWLLLDDAHGFGVLGNQGRGSLAHFNISSPRIIY
EEHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCEEEEECCCCEEEE
MGTLGKAAGVFGAFVAAQEEIIETLIQCARSYIYTTATPPFLSHALLKSLELIAGGAGRR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCH
EKLAQLTKLLKQECHPLRWQLLPSDTPIQPLVMGENAEALQVSEALRQKGILVTAIRPPT
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCEEEEEECCCC
VPEGTARLRISLSSSHDIEDVMELGAALREIDRDME
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA