Click here to switch to the map view.

The map label for this gene is bioH

Identifier: 82702260

GI number: 82702260

Start: 1300692

End: 1301483

Strand: Direct

Name: bioH

Synonym: Nmul_A1131

Alternate gene names: 82702260

Gene position: 1300692-1301483 (Clockwise)

Preceding gene: 82702259

Following gene: 82702261

Centisome position: 40.85

GC content: 55.56

Gene sequence:

>792_bases
ATGGGCCAGGTAAGCCTTTACGTCGAATCCTTGGGTGAAGGTCCCGATCTCGTTCTGCTGCATGGGTGGGCCATGCATAG
CGGAATGTGGGGTAGCACCCGGCGATCACTGGCCCAGCATTTCCGCTTGCATCTCGTCGATTTGCCGGGTCATGGATTCA
GCCGGGGAGCCTTACCTTACAAAAGGGGAGAGAAAAATGGTGTCAGCGAGGATATGGTCGAGAGGGTGGTGGAGGTTCTG
CCTCCCGATTGTGTAATTTGTGGATGGTCGCTGGGGGGACAGCTCGCAATCGAACTCGCCTTGCGCGAGCCGGCGCGCGT
GGAGAAAATCATTTTGACTTCCACCACGCCCAGCTTCGTCAAACGCGAGGACTGGCAATGGGGGATGGAGGAATTGACAT
TGAAGGCGTTTGCGGAAAATCTCAGGCGGGACTTCAGTACCACCATGAAGCGGTTTCTTACCCTGCAGGTGAGTGGGGGA
GGGGATGCCGGGAAGGTATTGCCCGAGATGCGGAGACTTTTGTTCGAGCGCAGTGCGCCGGAGCCTGAAGCATTGGAAGC
GGGGTTACAGATCGTGCTTGCGAACGACCTTCGCGGAAAGCTGCGAAACATCGTTCAACCCACTTTGCTGATACACGGCG
AGAACGATGTCATTGCCCATCCCGAGGCAGCGGCATGGATGAAGCAGCAATTTCAGGATGTCGAGCTGGCCATGCTACCG
AATTGCAGTCATGTGCCTTTCTTGTCCTATCCTGATAAATTCATTGCGAATATCGTTCGCTTCGCCAGATGA

Upstream 100 bases:

>100_bases
ACCGGGATATGGAATGACTATGGAATGACTGGGCACCTTTACGCCGGATATGACATTCGCCTTGTAAGGGCGACCTCGCG
GATACAATGTGGAATGCTCG

Downstream 100 bases:

>100_bases
GTTCAAATGAGTGGAAATGAGTCTGTAGTGAATTACGATCACTTCATCGATAAGCGCCGGGTGCGAAAGGCGTTTGAACG
GGCTGCGCCTTTATATGATC

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MGQVSLYVESLGEGPDLVLLHGWAMHSGMWGSTRRSLAQHFRLHLVDLPGHGFSRGALPYKRGEKNGVSEDMVERVVEVL
PPDCVICGWSLGGQLAIELALREPARVEKIILTSTTPSFVKREDWQWGMEELTLKAFAENLRRDFSTTMKRFLTLQVSGG
GDAGKVLPEMRRLLFERSAPEPEALEAGLQIVLANDLRGKLRNIVQPTLLIHGENDVIAHPEAAAWMKQQFQDVELAMLP
NCSHVPFLSYPDKFIANIVRFAR

Sequences:

>Translated_263_residues
MGQVSLYVESLGEGPDLVLLHGWAMHSGMWGSTRRSLAQHFRLHLVDLPGHGFSRGALPYKRGEKNGVSEDMVERVVEVL
PPDCVICGWSLGGQLAIELALREPARVEKIILTSTTPSFVKREDWQWGMEELTLKAFAENLRRDFSTTMKRFLTLQVSGG
GDAGKVLPEMRRLLFERSAPEPEALEAGLQIVLANDLRGKLRNIVQPTLLIHGENDVIAHPEAAAWMKQQFQDVELAMLP
NCSHVPFLSYPDKFIANIVRFAR
>Mature_262_residues
GQVSLYVESLGEGPDLVLLHGWAMHSGMWGSTRRSLAQHFRLHLVDLPGHGFSRGALPYKRGEKNGVSEDMVERVVEVLP
PDCVICGWSLGGQLAIELALREPARVEKIILTSTTPSFVKREDWQWGMEELTLKAFAENLRRDFSTTMKRFLTLQVSGGG
DAGKVLPEMRRLLFERSAPEPEALEAGLQIVLANDLRGKLRNIVQPTLLIHGENDVIAHPEAAAWMKQQFQDVELAMLPN
CSHVPFLSYPDKFIANIVRFAR

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=256, Percent_Identity=32.421875, Blast_Score=144, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_NITMU (Q2Y9Y7)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411826.1
- HSSP:   P13001
- ProteinModelPortal:   Q2Y9Y7
- STRING:   Q2Y9Y7
- GeneID:   3784244
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1131
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   FISHPRE
- PhylomeDB:   Q2Y9Y7
- ProtClustDB:   CLSK586187
- BioCyc:   NMUL323848:NMUL_A1131-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- InterPro:   IPR000639
- PRINTS:   PR00111
- PRINTS:   PR00412
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 29449; Mature: 29318

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: ACT_SITE 90-90 ACT_SITE 216-216 ACT_SITE 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVSLYVESLGEGPDLVLLHGWAMHSGMWGSTRRSLAQHFRLHLVDLPGHGFSRGALPY
CCCCEEEHHHCCCCCCEEEEECHHHHCCCCCHHHHHHHHHHEEEEEECCCCCCCCCCCCC
KRGEKNGVSEDMVERVVEVLPPDCVICGWSLGGQLAIELALREPARVEKIILTSTTPSFV
CCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCHHEEEEEECCCHHHH
KREDWQWGMEELTLKAFAENLRRDFSTTMKRFLTLQVSGGGDAGKVLPEMRRLLFERSAP
HCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCC
EPEALEAGLQIVLANDLRGKLRNIVQPTLLIHGENDVIAHPEAAAWMKQQFQDVELAMLP
CHHHHHHCEEEEEEHHHHHHHHHHCCCEEEEECCCCEEECCHHHHHHHHHHHCCCEEECC
NCSHVPFLSYPDKFIANIVRFAR
CCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GQVSLYVESLGEGPDLVLLHGWAMHSGMWGSTRRSLAQHFRLHLVDLPGHGFSRGALPY
CCCEEEHHHCCCCCCEEEEECHHHHCCCCCHHHHHHHHHHEEEEEECCCCCCCCCCCCC
KRGEKNGVSEDMVERVVEVLPPDCVICGWSLGGQLAIELALREPARVEKIILTSTTPSFV
CCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCHHEEEEEECCCHHHH
KREDWQWGMEELTLKAFAENLRRDFSTTMKRFLTLQVSGGGDAGKVLPEMRRLLFERSAP
HCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCC
EPEALEAGLQIVLANDLRGKLRNIVQPTLLIHGENDVIAHPEAAAWMKQQFQDVELAMLP
CHHHHHHCEEEEEEHHHHHHHHHHCCCEEEEECCCCEEECCHHHHHHHHHHHCCCEEECC
NCSHVPFLSYPDKFIANIVRFAR
CCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA