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The map label for this gene is mtkB [H]

Identifier: 82702209

GI number: 82702209

Start: 1246468

End: 1247355

Strand: Direct

Name: mtkB [H]

Synonym: Nmul_A1080

Alternate gene names: 82702209

Gene position: 1246468-1247355 (Clockwise)

Preceding gene: 82702208

Following gene: 82702210

Centisome position: 39.14

GC content: 53.6

Gene sequence:

>888_bases
ATGGCGATTTTAATTGATGAGAACACACGGATCATAGTTCAGGGTTTCACCGGCAAGATCGGTACCTTTCATGCACAGGA
TATGATCAATTACGGTTCCAACATTGTCGGCGGCGTAACCCCCGGTAAAGGGGGGCAACAGCATCTGGGGCGACCGGTTT
TCAATACGGTGAAAGAGGCTGTTCAGCAAGTCGGTGCGAATGCAAGCATCGTGTTTGTGCCTCCGGCATTTGCAGCGGAC
TCGATCATGGAAGCAGCCGACGCAGGCATCAAATATTGCGTTGCCATTACTGACGGTATTCCTACGCAGGATATGATGAC
GGTCAAGAACTTTTTACGCCGCTTTCCTATCGAAGAACGCATGGTACTTACCGGGCCGAATTGTGCGGGTACGATCAGCC
CGGGCAGGGCGATGCTGGGAATCATGCCGGGCCATATCTACATGGCGGGTGATGTGGGCGTCGTTGGCCGTTCCGGAACC
TTGGGGTATGAAGCCGCAGATCAAATGCGGACATTGGGCATCGGCATATCGACATCGGTCGGGATTGGCGGAGACCCGAT
TATTGGCAGTTCCCATCGCGATATTCTGGAGAGATTCGAAGAGGATCCCGAAACAAAAGTAGTGGTCATGATTGGAGAAA
TTGGAGGACCCCAGGAAGTCGAGGCAGGCATTTTCTCCAGAGACTACATGAGCAAACCCCTGGTGGCGTATATTGCCGGA
CTGACCGCACCGGAAGGGCGTCGCATGGGACATGCGGGTGCGATTATCTCCGCGGCGGGTGAAAGCGCTGCGGAAAAAGT
TGCCATCTTGAAAGAACTTGGGGTCACCATTTCCCCCACTCCTTCAGCCATAGGGGAGACAGTCGCAAAGGTCTTGGCTA
AGATGTAA

Upstream 100 bases:

>100_bases
CGGTTTGCCGATCATCACAGCGGGAACGCTGGCGGAAGCAGCGGAGAAGGTTGTCCAGGCGCGCAATGGCGCGGTTGCGG
AAGAGTGCAAAGGGATATAA

Downstream 100 bases:

>100_bases
GCGCGCAAAAGATTTTGCCCCGCACTTTTTGAAATGAACGTGGAGGCTTGATATGTCAATCTGGATGACAGCGTTAAAAA
TCATTCCCTGGGGGAATGTG

Product: succinyl-CoA synthetase subunit alpha

Products: NA

Alternate protein names: MTK-alpha; Malate thiokinase; Malyl-CoA synthetase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAILIDENTRIIVQGFTGKIGTFHAQDMINYGSNIVGGVTPGKGGQQHLGRPVFNTVKEAVQQVGANASIVFVPPAFAAD
SIMEAADAGIKYCVAITDGIPTQDMMTVKNFLRRFPIEERMVLTGPNCAGTISPGRAMLGIMPGHIYMAGDVGVVGRSGT
LGYEAADQMRTLGIGISTSVGIGGDPIIGSSHRDILERFEEDPETKVVVMIGEIGGPQEVEAGIFSRDYMSKPLVAYIAG
LTAPEGRRMGHAGAIISAAGESAAEKVAILKELGVTISPTPSAIGETVAKVLAKM

Sequences:

>Translated_295_residues
MAILIDENTRIIVQGFTGKIGTFHAQDMINYGSNIVGGVTPGKGGQQHLGRPVFNTVKEAVQQVGANASIVFVPPAFAAD
SIMEAADAGIKYCVAITDGIPTQDMMTVKNFLRRFPIEERMVLTGPNCAGTISPGRAMLGIMPGHIYMAGDVGVVGRSGT
LGYEAADQMRTLGIGISTSVGIGGDPIIGSSHRDILERFEEDPETKVVVMIGEIGGPQEVEAGIFSRDYMSKPLVAYIAG
LTAPEGRRMGHAGAIISAAGESAAEKVAILKELGVTISPTPSAIGETVAKVLAKM
>Mature_294_residues
AILIDENTRIIVQGFTGKIGTFHAQDMINYGSNIVGGVTPGKGGQQHLGRPVFNTVKEAVQQVGANASIVFVPPAFAADS
IMEAADAGIKYCVAITDGIPTQDMMTVKNFLRRFPIEERMVLTGPNCAGTISPGRAMLGIMPGHIYMAGDVGVVGRSGTL
GYEAADQMRTLGIGISTSVGIGGDPIIGSSHRDILERFEEDPETKVVVMIGEIGGPQEVEAGIFSRDYMSKPLVAYIAGL
TAPEGRRMGHAGAIISAAGESAAEKVAILKELGVTISPTPSAIGETVAKVLAKM

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the succinate/malate CoA ligase alpha subunit family [H]

Homologues:

Organism=Homo sapiens, GI109452591, Length=296, Percent_Identity=53.3783783783784, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI38569423, Length=266, Percent_Identity=30.0751879699248, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI38569421, Length=266, Percent_Identity=30.0751879699248, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1786949, Length=292, Percent_Identity=56.8493150684932, Blast_Score=321, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17553126, Length=294, Percent_Identity=48.9795918367347, Blast_Score=266, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17550194, Length=291, Percent_Identity=48.7972508591065, Blast_Score=263, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17551266, Length=321, Percent_Identity=24.9221183800623, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557344, Length=265, Percent_Identity=25.2830188679245, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324716, Length=301, Percent_Identity=42.8571428571429, Blast_Score=242, Evalue=4e-65,
Organism=Drosophila melanogaster, GI21356559, Length=297, Percent_Identity=47.4747474747475, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI28574296, Length=297, Percent_Identity=46.8013468013468, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI281363475, Length=268, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281363473, Length=268, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281363477, Length=268, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017440
- InterPro:   IPR003781
- InterPro:   IPR005810
- InterPro:   IPR005811
- InterPro:   IPR016040
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: =6.2.1.9 [H]

Molecular weight: Translated: 30771; Mature: 30640

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00399 SUCCINYL_COA_LIG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILIDENTRIIVQGFTGKIGTFHAQDMINYGSNIVGGVTPGKGGQQHLGRPVFNTVKEA
CEEEEECCCEEEEEECCCCCCCCHHHHHHHCCCCEECCCCCCCCCHHHCCCHHHHHHHHH
VQQVGANASIVFVPPAFAADSIMEAADAGIKYCVAITDGIPTQDMMTVKNFLRRFPIEER
HHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCC
MVLTGPNCAGTISPGRAMLGIMPGHIYMAGDVGVVGRSGTLGYEAADQMRTLGIGISTSV
EEEECCCCCCCCCCCCEEEEECCCEEEEECCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
GIGGDPIIGSSHRDILERFEEDPETKVVVMIGEIGGPQEVEAGIFSRDYMSKPLVAYIAG
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHH
LTAPEGRRMGHAGAIISAAGESAAEKVAILKELGVTISPTPSAIGETVAKVLAKM
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AILIDENTRIIVQGFTGKIGTFHAQDMINYGSNIVGGVTPGKGGQQHLGRPVFNTVKEA
EEEEECCCEEEEEECCCCCCCCHHHHHHHCCCCEECCCCCCCCCHHHCCCHHHHHHHHH
VQQVGANASIVFVPPAFAADSIMEAADAGIKYCVAITDGIPTQDMMTVKNFLRRFPIEER
HHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCC
MVLTGPNCAGTISPGRAMLGIMPGHIYMAGDVGVVGRSGTLGYEAADQMRTLGIGISTSV
EEEECCCCCCCCCCCCEEEEECCCEEEEECCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
GIGGDPIIGSSHRDILERFEEDPETKVVVMIGEIGGPQEVEAGIFSRDYMSKPLVAYIAG
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHH
LTAPEGRRMGHAGAIISAAGESAAEKVAILKELGVTISPTPSAIGETVAKVLAKM
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961516 [H]