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The map label for this gene is potB [H]

Identifier: 82702110

GI number: 82702110

Start: 1141099

End: 1141998

Strand: Reverse

Name: potB [H]

Synonym: Nmul_A0981

Alternate gene names: 82702110

Gene position: 1141998-1141099 (Counterclockwise)

Preceding gene: 82702111

Following gene: 82702109

Centisome position: 35.86

GC content: 55.67

Gene sequence:

>900_bases
ATGAAAGAAAAGCAGCTTGCCCGGCTGCTGGTGAGCGGGCCACCCCTATTGTTCCTGATAGTGTTCTTTGTGGCACCCAG
CCTGATCATGATTCTCACCTCGTTTCGCTTTCCGGGTGAATTCGGCGGACTGGCCCCCGTTATCCCGCCTTCGGACCAAG
CGATTGGCGAATATGGATTCACCTCGGACGCATATCAGTTCTTTTTCAGCGACTTCATCTACACGGAGATCTTTCTCAAA
TCGTTCGCGGTTGCGTTCGGCACGACCCTGATCTGCCTCATCCTGGCTTATCCATTGGCAGTACTGATTGCCCGCAGCAG
AAAACACTTGCGCAACCTGATGGTCCTGTTGGTGATACTTCCGTTCGCGAGCAATTTCCTCATTCGCATCTACGCGTGGA
TGATCATTCTCGGCCCGGAATCCGCGCTCAGCCACTTTGTCAACACCCTTCTCGGCGCGTTCGGAATCGCCCCGGTCACG
TTATTATTCTCGCCTTTTGCAGTATTGGTGGGCATGGTATACGTACATCTGCCCTTCATGGTGCTGCCTCTCTATACCAA
TCTGGAGAAGCACGATCCCTCCCTTCTGGATGCGGCACAGGATCTGGGGGCGAATGCCTGGCAACGCTTCTGGCGGGTTA
CCTGGCCGCTGTCCCTGCCGGGCGTATTTTCAGGATCCGCGCTGGTATTCATTCCGGTGCTGGGCATGTTCGCCGTGCCG
GATATATTAGGCGGCACAGGCGATATCCTGATCGGAAACCTGATCAAGGATCAGTTCCTGGGCACTCGCGACTGGCCTTT
CGGTTCTGCATTGTCCATCATGCTGACACTGGCGGTACTATCCGTAGCAGGCTTGACGACATGGTTCGCCCGTTCCGCAA
CCGGCCAGCGGGCGATATAG

Upstream 100 bases:

>100_bases
CTGGCCGGGCCAAGTTTTTCGAGGTGGATGACGAGGTAACAATCTCCTGGCGCCACGACGCGGGCATTTTTCTCAATGAT
CCTGAATGATTAAGGGCGAA

Downstream 100 bases:

>100_bases
CGTGAAGCGCGGCAATCCCTGGTTGTGGTCCGCGGCCGTGCTGGTTTACGCCTTCCTTTATATCCCGCTGATCATCGTAG
TGCTGTACTCGTTCAACGAT

Product: binding-protein dependent transport system inner membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKEKQLARLLVSGPPLLFLIVFFVAPSLIMILTSFRFPGEFGGLAPVIPPSDQAIGEYGFTSDAYQFFFSDFIYTEIFLK
SFAVAFGTTLICLILAYPLAVLIARSRKHLRNLMVLLVILPFASNFLIRIYAWMIILGPESALSHFVNTLLGAFGIAPVT
LLFSPFAVLVGMVYVHLPFMVLPLYTNLEKHDPSLLDAAQDLGANAWQRFWRVTWPLSLPGVFSGSALVFIPVLGMFAVP
DILGGTGDILIGNLIKDQFLGTRDWPFGSALSIMLTLAVLSVAGLTTWFARSATGQRAI

Sequences:

>Translated_299_residues
MKEKQLARLLVSGPPLLFLIVFFVAPSLIMILTSFRFPGEFGGLAPVIPPSDQAIGEYGFTSDAYQFFFSDFIYTEIFLK
SFAVAFGTTLICLILAYPLAVLIARSRKHLRNLMVLLVILPFASNFLIRIYAWMIILGPESALSHFVNTLLGAFGIAPVT
LLFSPFAVLVGMVYVHLPFMVLPLYTNLEKHDPSLLDAAQDLGANAWQRFWRVTWPLSLPGVFSGSALVFIPVLGMFAVP
DILGGTGDILIGNLIKDQFLGTRDWPFGSALSIMLTLAVLSVAGLTTWFARSATGQRAI
>Mature_299_residues
MKEKQLARLLVSGPPLLFLIVFFVAPSLIMILTSFRFPGEFGGLAPVIPPSDQAIGEYGFTSDAYQFFFSDFIYTEIFLK
SFAVAFGTTLICLILAYPLAVLIARSRKHLRNLMVLLVILPFASNFLIRIYAWMIILGPESALSHFVNTLLGAFGIAPVT
LLFSPFAVLVGMVYVHLPFMVLPLYTNLEKHDPSLLDAAQDLGANAWQRFWRVTWPLSLPGVFSGSALVFIPVLGMFAVP
DILGGTGDILIGNLIKDQFLGTRDWPFGSALSIMLTLAVLSVAGLTTWFARSATGQRAI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=281, Percent_Identity=40.5693950177936, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787080, Length=240, Percent_Identity=42.0833333333333, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787713, Length=209, Percent_Identity=32.5358851674641, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32812; Mature: 32812

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKQLARLLVSGPPLLFLIVFFVAPSLIMILTSFRFPGEFGGLAPVIPPSDQAIGEYGF
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
TSDAYQFFFSDFIYTEIFLKSFAVAFGTTLICLILAYPLAVLIARSRKHLRNLMVLLVIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFASNFLIRIYAWMIILGPESALSHFVNTLLGAFGIAPVTLLFSPFAVLVGMVYVHLPFM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLPLYTNLEKHDPSLLDAAQDLGANAWQRFWRVTWPLSLPGVFSGSALVFIPVLGMFAVP
HHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
DILGGTGDILIGNLIKDQFLGTRDWPFGSALSIMLTLAVLSVAGLTTWFARSATGQRAI
HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]