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The map label for this gene is usf [H]

Identifier: 82702043

GI number: 82702043

Start: 1041258

End: 1042049

Strand: Direct

Name: usf [H]

Synonym: Nmul_A0914

Alternate gene names: 82702043

Gene position: 1041258-1042049 (Clockwise)

Preceding gene: 82702036

Following gene: 82702045

Centisome position: 32.7

GC content: 55.18

Gene sequence:

>792_bases
ATGAAAAAAATCCTCTCAGCCGCTGCATTGTTTTTCCTGGGTTCGCTGGCCCAGGCTGCCATACAGGGGAAGGAAGTCAC
CTACACCACCAATGGCACCACCCTCAAAGGTTACCTTGTATATGATGACGCAATAACACATAACCACCCTGCGGTGCTGG
TGGTGCATGAATGGTGGGGTCTCGACGATTACGCGCGGAAACGCGCAAAGATGCTGGCGGAACTGGGCTACACCGCGTTG
GCAGTCGACATGTACGGCGAGGGCAGGCAGGCTCATCATCCGGATGAGGCCTCCAGATATTCGGGAGAGCTGAAGAAGAA
TCTGCCCTTGGCGAAAAAACGCTTTGAAGCTGCCCTGAGTTTCCTGCAGAAGCAGGAGAATGTCGATCCGAGGAACATCG
CGGCGCTGGGTTATTGTTTCGGCGGCAGCGTCGCCTTGGAAATGGCTCGCCAAGGTGAAGATTTGAAAGGTGTGGCCAGC
TTTCATGGCGGTCTGGCGACCGAGCACCCGGCTCAAAGGGGCAGGGTGAAGGCGCAGATTATCTCTTTTACCGGCATGGC
TGACCCCATGATACCGGCCGAGCAGGTAGCGGCATTCAAGCAGGAAATGGAGAATGCCGGCGTAAACTACAAGGCAGTTA
CATTTCCGGGCGCCAAGCATAGTTTTACCAATCCCGAGGCCGATGACTACGGGCGCAAATTCAATTTACCGCTCGCTTAT
GACGCGCAAGCTGACAAGGCTTCTTGGAAGGAGACGGAACGCTTCCTGGCCGAGGTATTCAAGGCAAGATGA

Upstream 100 bases:

>100_bases
GATTCCATCGTTCGTTATTTTCGATTTGGGTGGCCCGCAGGCCTCGCCTATACTCACATCATCAATAAATTTAATCAACC
ACCCGCACAATGAGCTCATC

Downstream 100 bases:

>100_bases
TGATGCCCCGAAGTATACTGGTTTTTGTAGTCTCGAATGAGCGAGGGTTGGAAACGCCTGCACGACGTGCGGGACTTATG
CGGCAATCAGGATATTTGCG

Product: dienelactone hydrolase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKKILSAAALFFLGSLAQAAIQGKEVTYTTNGTTLKGYLVYDDAITHNHPAVLVVHEWWGLDDYARKRAKMLAELGYTAL
AVDMYGEGRQAHHPDEASRYSGELKKNLPLAKKRFEAALSFLQKQENVDPRNIAALGYCFGGSVALEMARQGEDLKGVAS
FHGGLATEHPAQRGRVKAQIISFTGMADPMIPAEQVAAFKQEMENAGVNYKAVTFPGAKHSFTNPEADDYGRKFNLPLAY
DAQADKASWKETERFLAEVFKAR

Sequences:

>Translated_263_residues
MKKILSAAALFFLGSLAQAAIQGKEVTYTTNGTTLKGYLVYDDAITHNHPAVLVVHEWWGLDDYARKRAKMLAELGYTAL
AVDMYGEGRQAHHPDEASRYSGELKKNLPLAKKRFEAALSFLQKQENVDPRNIAALGYCFGGSVALEMARQGEDLKGVAS
FHGGLATEHPAQRGRVKAQIISFTGMADPMIPAEQVAAFKQEMENAGVNYKAVTFPGAKHSFTNPEADDYGRKFNLPLAY
DAQADKASWKETERFLAEVFKAR
>Mature_263_residues
MKKILSAAALFFLGSLAQAAIQGKEVTYTTNGTTLKGYLVYDDAITHNHPAVLVVHEWWGLDDYARKRAKMLAELGYTAL
AVDMYGEGRQAHHPDEASRYSGELKKNLPLAKKRFEAALSFLQKQENVDPRNIAALGYCFGGSVALEMARQGEDLKGVAS
FHGGLATEHPAQRGRVKAQIISFTGMADPMIPAEQVAAFKQEMENAGVNYKAVTFPGAKHSFTNPEADDYGRKFNLPLAY
DAQADKASWKETERFLAEVFKAR

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI17536419, Length=269, Percent_Identity=33.457249070632, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17532655, Length=214, Percent_Identity=35.5140186915888, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17532653, Length=223, Percent_Identity=34.5291479820628, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI193204305, Length=213, Percent_Identity=30.5164319248826, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17536415, Length=270, Percent_Identity=28.1481481481481, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534829, Length=151, Percent_Identity=35.0993377483444, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 28977; Mature: 28977

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILSAAALFFLGSLAQAAIQGKEVTYTTNGTTLKGYLVYDDAITHNHPAVLVVHEWWG
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCCCCEEEEEECCCC
LDDYARKRAKMLAELGYTALAVDMYGEGRQAHHPDEASRYSGELKKNLPLAKKRFEAALS
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHCCHHHHCCCHHHHHHHHHHH
FLQKQENVDPRNIAALGYCFGGSVALEMARQGEDLKGVASFHGGLATEHPAQRGRVKAQI
HHHHHCCCCHHHHEEEHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHCCEEEEE
ISFTGMADPMIPAEQVAAFKQEMENAGVNYKAVTFPGAKHSFTNPEADDYGRKFNLPLAY
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHCCCEECCCEEE
DAQADKASWKETERFLAEVFKAR
CCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKILSAAALFFLGSLAQAAIQGKEVTYTTNGTTLKGYLVYDDAITHNHPAVLVVHEWWG
CHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCCCCEEEEEECCCC
LDDYARKRAKMLAELGYTALAVDMYGEGRQAHHPDEASRYSGELKKNLPLAKKRFEAALS
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHCCHHHHCCCHHHHHHHHHHH
FLQKQENVDPRNIAALGYCFGGSVALEMARQGEDLKGVASFHGGLATEHPAQRGRVKAQI
HHHHHCCCCHHHHEEEHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHCCEEEEE
ISFTGMADPMIPAEQVAAFKQEMENAGVNYKAVTFPGAKHSFTNPEADDYGRKFNLPLAY
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHCCCEECCCEEE
DAQADKASWKETERFLAEVFKAR
CCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]